Abstract:
BACKGROUND:In myocardial cells, relaxation is governed primarily by the sarcoplasmic reticulum (SR) Ca(2+)-ATPase transporting enzyme, which regulates Ca2+ sequestration into the SR. Human and experimental cardiomyopathies are associated with reduced SR Ca(2+)-ATPase activity. METHODS AND RESULTS:To modify intracellular calcium mobilization, we created a recombinant adenovirus designed to over-express the cardiac SR Ca(2+)-ATPase (SERCA2a) under the control of the Rous sarcoma virus (RSV). In neonatal rat myocytes, Ad.RSV.SERCA2a increased the expression of SERCA2a in a concentration-dependent and time-dependent fashion. Enhancement of SR Ca(2+)-ATPase activity was even greater than increases in SERCA2a protein content in cells infected with Ad.RSV.SERCA2a for 48 hours at a multiplicity of infection (MOI) from 0.1 to 10.0 pfu/cell. Intracellular calcium transients measured in the neonatal cells infected with Ad.RSV.SERCA2a were characterized by an abbreviation of the relaxation phase, an increase in peak [Ca2+]i release, and a decrease in resting [Ca2+]i levels. Ad.RSV.SERCA2a also enhanced the contraction of the myocardial cells as detected by shortening measurements. CONCLUSIONS:We found that adenovirus-mediated gene transfer of SR Ca(2+)-ATPase can modify intracellular calcium handling and shortening in myocardial cells. Such vectors should be useful in examining the role of reduced SERCA2a activity in the pathophysiology of heart failure and in developing strategies for gene therapy.
journal_name
Circulationjournal_title
Circulationauthors
Hajjar RJ,Kang JX,Gwathmey JK,Rosenzweig Adoi
10.1161/01.cir.95.2.423subject
Has Abstractpub_date
1997-01-21 00:00:00pages
423-9issue
2eissn
0009-7322issn
1524-4539journal_volume
95pub_type
杂志文章相关文献
CIRCULATION文献大全abstract::Labetalol was administered to six hypertensive subjects in increasing doses for seven days. A decrease in both supine and standing arterial pressure and heart rate was observed with no change in cardiac output and few side effects. Exercise tolerance was unaltered by the drug, but the heart rate and arterial pressure ...
journal_title:Circulation
pub_type: 临床试验,杂志文章
doi:10.1161/01.cir.55.2.370
更新日期:1977-02-01 00:00:00
abstract:BACKGROUND:The identification of sites of dominant activation frequency during atrial fibrillation (AF) in humans and the effect of ablation at these sites have not been reported. METHODS AND RESULTS:Thirty-two patients undergoing AF ablation (19 paroxysmal, 13 permanent) during ongoing arrhythmia were studied. Electr...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.104.517011
更新日期:2005-08-09 00:00:00
abstract::The incidence of valvular heart disease is expected to increase over the next several decades as a large proportion of the US demographic advances into the later decades of life. At the same time, the next several years can be anticipated to bring a broad transition of surgical therapy to minimally invasive (minithora...
journal_title:Circulation
pub_type: 杂志文章,评审
doi:10.1161/CIRCULATIONAHA.107.188525
更新日期:2008-04-01 00:00:00
abstract::In 1948 knowledge of the role of the central nervous system in controlling blood pressure was rudimentary and the possibility that the brain could contribute to hypertension was barely acknowledged. Over the past 35 years the development of new technologies, particularly those of the neurosciences, and their applicati...
journal_title:Circulation
pub_type: 历史文章,杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract:BACKGROUND:Randomized clinical trials have shown that multivessel coronary angioplasty is feasible and provides similar long-term survival as bypass surgery in selected patients. However, the higher need for repeat intervention, in particular, coronary artery bypass graft surgery, remains a problem. The objective of th...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.96.11.3873
更新日期:1997-12-02 00:00:00
abstract:BACKGROUND:The risk of recurrent venous thrombosis is 2-fold higher in men than in women. In contrast, no such sex difference in the risk of first venous thrombosis has been reported. We hypothesized that, for a first event, a risk difference between the sexes is masked by female exposure to reproductive factors (oral ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.113.004768
更新日期:2014-01-07 00:00:00
abstract:BACKGROUND:Transplantation of ex vivo expanded circulating endothelial progenitor cells (EPCs) from peripheral blood mononuclear cells improves the neovascularization after critical ischemia. However, the origin of the endothelial progenitor lineage and its characteristics have not yet been clearly defined. Therefore, ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.CIR.0000096483.29777.50
更新日期:2003-11-18 00:00:00
abstract:BACKGROUND:Right ventricular (RV) function is the major determinant for both functional capacity and survival in patients with pulmonary arterial hypertension (PAH). Despite the recognized clinical importance of preserving RV function, the subcellular mechanisms that govern the transition from a compensated to a decomp...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.120.047626
更新日期:2020-10-13 00:00:00
abstract::Body surface isochrone mapping was performed in 36 normal subjects and in 85 patients with previous myocardial infarction. Eighty-seven unipolar electrocardiograms distributed over the anterior chest and the back were recorded simultaneously. For each lead, activation time was measured as the time from the onset of QR...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.72.4.801
更新日期:1985-10-01 00:00:00
abstract:BACKGROUND:Proto-oncogenes have been implicated in the pathogenesis of gene-mediated myocardial remodeling. In the human heart, however, it has not been clarified whether these proto-oncogenes are related to contractile impairment and structural alteration of the myocardium. The present study is designed to investigate...
journal_title:Circulation
pub_type: 杂志文章
doi:
更新日期:1997-11-04 00:00:00
abstract::Four revolutions are transforming the cardiology of our day. The first is a social revolution. It has resulted in plummeting esteem for the medical profession reflecting disenchantment coupled with the rapid emergence of the recognition that health care is a right rather than a privilege. The second revolution, interv...
journal_title:Circulation
pub_type: 传,历史文章,杂志文章
doi:10.1161/01.cir.87.6.2047
更新日期:1993-06-01 00:00:00
abstract:BACKGROUND:Whether reperfusion can cause necrosis of previously viable myocytes (lethal reperfusion injury) remains controversial. Numerous studies examined the ability of various agents to prevent or limit reperfusion injury, but the results were contradictory. In a single-canine-heart model of ischemia-reperfusion, w...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.91.12.2989
更新日期:1995-06-15 00:00:00
abstract:BACKGROUND:The detection and differentiation of intracardiac masses is still challenging and may include neoplasms and thrombi. The aim of this study was the investigation of a targeted, fibrin-specific contrast agent (EP-2104R) for molecular targeted magnetic resonance imaging (MRI) of left atrial clots. METHODS AND ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.104.529941
更新日期:2005-07-19 00:00:00
abstract::The modulated receptor hypothesis states that sodium channels have a specific receptor for antiarrhythmic drugs. Therefore, two agents that block sodium channels by binding to this receptor are expected to compete for occupancy. Glycylxylidide (GX) is a deethylated metabolite of lidocaine that accumulates in patients ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.78.3.692
更新日期:1988-09-01 00:00:00
abstract:BACKGROUND:Varicose veins are a common problem with no approved medical therapies. Although it is believed that varicose vein pathogenesis is multifactorial, there is limited understanding of the genetic and environmental factors that contribute to their formation. Large-scale studies of risk factors for varicose veins...
journal_title:Circulation
pub_type: 杂志文章,多中心研究
doi:10.1161/CIRCULATIONAHA.118.035584
更新日期:2018-12-18 00:00:00
abstract:BACKGROUND:Pharmacogenetic-guided dosing of warfarin is a promising application of "personalized medicine" but has not been adequately tested in randomized trials. METHODS AND RESULTS:Consenting patients (n=206) being initiated on warfarin were randomized to pharmacogenetic-guided or standard dosing. Buccal swab DNA w...
journal_title:Circulation
pub_type: 杂志文章,随机对照试验
doi:10.1161/CIRCULATIONAHA.107.737312
更新日期:2007-11-27 00:00:00
abstract:BACKGROUND:We investigated the association between trainees performing supervised operations and late outcomes of patients undergoing cardiac surgery. METHODS AND RESULTS:Data were prospectively collected on patients who underwent coronary artery bypass graft surgery, aortic valve replacement, or a combination of thes...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.107.756379
更新日期:2008-09-30 00:00:00
abstract:BACKGROUND:In cardiac arrest, use of percutaneous cardiopulmonary bypass support (PCPS) may lead to left ventricular loading, with deleterious effects on the myocardium, and is often accompanied by an increase in pulmonary artery pressure. The present study was designed to assess the potential of artificially induced p...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.91.10.2664
更新日期:1995-05-15 00:00:00
abstract:BACKGROUND:Perivascular adipose tissue (PVAT) surrounds most vessels and shares common features with brown adipose tissue (BAT). Although adaptive thermogenesis in BAT increases energy expenditure and is beneficial for metabolic diseases, little is known about the role of PVAT in vascular diseases such as atheroscleros...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.112.104489
更新日期:2012-08-28 00:00:00
abstract:BACKGROUND:Each month, the number of transplant candidates added to the waiting list exceeds the number of transplantations performed, and many outpatients deteriorate to require transplantation urgently. The current list of 2400 candidates and the average wait of 8 months continue to increase. METHODS AND RESULTS:To ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.89.1.450
更新日期:1994-01-01 00:00:00
abstract::The importance of pulmonary artery size and the value of a standardized pulmonary artery index (PAI) in predicting outcome after Fontan's repair have previously been reported and questioned. We retrospectively reviewed 29 patients undergoing modified Fontan repair at The Children's Hospital of Philadelphia to examine ...
journal_title:Circulation
pub_type: 杂志文章
doi:
更新日期:1989-09-01 00:00:00
abstract:BACKGROUND:Mild oxidation of LDL enhances its atherogenic potential and induces a synergistic interaction with serotonin (5HT) on vascular smooth muscle cell (VSMC) proliferation. Because of its complex chemical nature, the mitogenic components of mildly oxidized LDL (moxLDL) remain unclear. METHODS AND RESULTS:We exa...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.103.10.1440
更新日期:2001-03-13 00:00:00
abstract:BACKGROUND:Phosphatidylserine-expressing microparticles circulate in blood with a short half-life of <10 minutes. We tested the role of an endothelium-derived phosphatidylserine-binding opsonin, developmental endothelial locus-1 (Del-1), in the uptake of platelet microparticles. METHODS AND RESULTS:Cultured human umbi...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.111.068833
更新日期:2012-04-03 00:00:00
abstract:BACKGROUND:Previous studies found that percutaneous coronary intervention (PCI) does not improve outcome compared with medical therapy (MT) in patients with stable coronary artery disease, but PCI was guided by angiography alone. FAME 2 trial (Fractional Flow Reserve Versus Angiography for Multivessel Evaluation) compa...
journal_title:Circulation
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1161/CIRCULATIONAHA.117.031907
更新日期:2018-01-30 00:00:00
abstract:BACKGROUND:Vascular smooth muscle cell (VMSC) proliferation is an essential component of myointimal hyperplasia, which is implicated in the failure of 30% to 50% of vascular interventions, such as coronary angioplasty and peripheral vein grafting. We have shown that cells derived from stenotic lesions in infrainguinal ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.97.25.2506
更新日期:1998-06-30 00:00:00
abstract:OBJECTIVE:To assess the outcome of 3 different surgical approaches for treatment of acute aortic dissection type A (AADA). METHODS AND RESULTS:Between October 1990 and October 2003, we operated on 295 patients (pts) for AADA. Follow-up was complete for 257 pts (87%). Supracommissural replacement (SCR) of the ascending...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.CIR.0000138948.14144.d6
更新日期:2004-09-14 00:00:00
abstract:BACKGROUND:Dual antiplatelet therapy in older versus younger patients with acute coronary syndromes is understudied. Low-dose prasugrel (5 mg/d) is recommended for younger, lower-body-weight patients and elderly patients with acute coronary syndromes to mitigate the bleeding risk of standard-dose prasugrel (10 mg/d). ...
journal_title:Circulation
pub_type: 杂志文章,随机对照试验
doi:10.1161/CIRCULATIONAHA.113.002303
更新日期:2013-08-20 00:00:00
abstract:BACKGROUND:Chemokines play an essential role in regulating the infiltration of leukocytes into allografts in experimental models. Little is known of their expression or function after human cardiac transplantation. METHODS AND RESULTS:We analyzed 169 sequential human endomyocardial biopsies by immunocytochemistry for ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/hc4601.098010
更新日期:2001-11-20 00:00:00
abstract:BACKGROUND:Left ventricular noncompaction is a cardiomyopathy characterized by excessive trabeculation of the left ventricle, progressive myocardial dysfunction, and early mortality. Left ventricular noncompaction has a heterogeneous clinical presentation that includes arrhythmia and sudden cardiac death. METHODS AND ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.113.002511
更新日期:2013-06-04 00:00:00
abstract:BACKGROUND:The safety and effectiveness of the MitraClip device (Abbott Vascular, Menlo Park, CA) is being evaluated in the Endovascular Valve Edge-to-Edge Repair Study (EVEREST) clinical studies. The healing response after device implantation has not previously been characterized in humans. METHODS AND RESULTS:A tota...
journal_title:Circulation
pub_type: 杂志文章,随机对照试验
doi:10.1161/CIRCULATIONAHA.110.978130
更新日期:2011-04-05 00:00:00