The replica technique used to study superficial corneal epithelium in vivo.

Abstract:

:We used 2.15 to 2.3% collodion in amyl acetate to form a replica of the corneal surface epithelium in vivo in seven rabbit eyes and ten human eyes. When it was peeled off the replica removed superficial epithelial cells in patches. The corneal epithelium regenerated within one week. Repeated applications at weekly intervals for three months produced no harmful long-term effects in five rabbit corneas as evaluated with slit-lamp biomicroscopy, histological methods, and vital staining of the endothelium. In ten human eyes, the technique was harmless and chemically cauterized the dendritic herpetic ulcers. We studied the replicas and cells by conventional histological methods and electron microscopy. The preservation of cells was fairly good even for ultrastructural study.

journal_name

Am J Ophthalmol

authors

Missotten L,Maudgal PC

doi

10.1016/0002-9394(77)90333-6

subject

Has Abstract

pub_date

1977-07-01 00:00:00

pages

104-11

issue

1

eissn

0002-9394

issn

1879-1891

pii

0002-9394(77)90333-6

journal_volume

84

pub_type

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