Abstract：
PURPOSE:To use micro-ribonucleic acid (microRNA) profiles in the vitreous for differential diagnosis of primary vitreoretinal lymphoma and uveitis. DESIGN:Prospective cross-sectional study. METHODS:This prospective cross-sectional study included 17 diffuse large B-cell primary vitreoretinal lymphoma and 12 uveitis patients. The supernatant of ocular fluid was subjected to total RNA extraction, followed by complementary deoxyribonucleic acid (cDNA) synthesis. Selected samples (primary vitreoretinal lymphoma, n = 3; uveitis, n = 3) were arrayed by a real-time polymerase chain reaction (RT-PCR)-based microRNA panel that detects 168 human mature microRNAs. The markers promising in distinct levels between uveitis and lymphoma were further tested for in all the other 23 samples by individual RT-PCR analysis. RESULTS:Of 168 microRNAs in the array, 66.5% were detectable with consistent higher microRNA-484, microRNA-197, and microRNA-132 in the primary vitreoretinal lymphoma vitreous and higher microRNA-155, microRNA-200c, and microRNA-22* in the uveitic ocular fluids. The results were normalized by different combinations of 7 control microRNAs (microRNA-103, microRNA-191, microRNA-42-5p, microRNA-16, microRNA-425, microRNA-93, and microRNA-451). After optimization, normalization against microRNA-16 was equally as reliable as the average of the 7 control microRNAs. Individual assays of all samples supported the pattern yielded from the array analysis. But only microRNA-155 was significantly higher in the uveitic vitreous compared to that with lymphoma. CONCLUSIONS:Mature microRNAs are detectable in ocular fluid samples. Primary vitreoretinal B-cell lymphoma and uveitis might be characterized by distinct microRNA signatures. Quantification of ocular microRNA-155 might be helpful in the differential diagnosis of these 2 diseases.
journal_name
Am J Ophthalmoljournal_title
American journal of ophthalmologyauthors
Tuo J,Shen D,Yang HH,Chan CCdoi
10.1016/j.ajo.2013.12.014subject
Has Abstractpub_date
2014-03-01 00:00:00pages
728-34issue
3eissn
0002-9394issn
1879-1891pii
S0002-9394(13)00784-8journal_volume
157pub_type
杂志文章abstract：PURPOSE:To determine whether treatment of macular retinoblastoma with chemotherapy and laser hyperthermia can lead to satisfactory visual acuity. METHODS:A child with unilateral macular retinoblastoma was treated with chemotherapy and laser hyperthermia. Visual function was assessed one year after treatment. RESULTS:...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(14)75441-8
更新日期：1996-05-01 00:00:00
abstract：PURPOSE:To investigate the relationship between abnormal contrast sensitivity and abnormal color vision among human immunodeficiency virus (HIV)-infected individuals who have normal visual acuity and to seek host factors that predict these abnormalities. DESIGN:Noninterventional, cross-sectional study. METHODS:We eva...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
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更新日期：2006-08-01 00:00:00
abstract：PURPOSE:To determine whether patients can guess their intraocular pressure (IOP). DESIGN:Patient survey. METHODS:We asked consecutive patients to guess their IOP and then indicate the IOP and the symptoms that allowed them to guess. RESULTS:Of 132 patients, 22 (17%) believed they could guess their IOP, usually based...
journal_title：American journal of ophthalmology
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更新日期：2004-02-01 00:00:00
abstract：:Two patients underwent vitrectomy with the use of intraocular gas. In one patient the cornea decompensated because of lens-cornea contact and in the second patient a surgical technique devised to protect the cornea from lens contact was used successfully. The technique consisted of injecting a smaller gas bubble into ...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(80)90284-6
更新日期：1980-05-01 00:00:00
abstract：:Four patients, two men 25 and 43 years old and two women 20 and 40 years old, had complete monocular temporal hemianopsias, suggesting that it may be a more common functional deficit than has been recognized. The initial symptoms were visual loss and headache, and eye pain also occurred. Correct diagnosis is possible ...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(86)90600-8
更新日期：1986-02-15 00:00:00
abstract：PURPOSE:To assess the efficacy and safety of adjunctive daunorubicin during vitrectomy surgery in eyes with idiopathic proliferative vitreoretinopathy (PVR). METHODS:Two hundred eighty-six eyes (286 patients) with stage C2 (Retina Society Classification, 1983) or more advanced preoperative PVR in which surgery with si...
journal_title：American journal of ophthalmology
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s0002-9394(98)00115-9
更新日期：1998-10-01 00:00:00
abstract：:A 61-year-old white woman, who had progressive arthritis over a two-year period, developed bilateral peripheral corneal degeneration. The superior cornea thinned to a thickness of approximately two thirds of normal. She died suddenly in the hospital and a primary occlusive vasculitis of the anterior ciliary arteries w...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
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更新日期：1978-03-01 00:00:00
abstract：:We performed a histologic study to support our clinical observations on the mechanisms responsible for traction retinal detachment after a penetrating injury in the rhesus monkey eye. The monkey eyes (40 eyes; 40 monkeys) were characterized by intraocular fibrosis with the formation of a cyclitic membrane and epiretin...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(79)90469-0
更新日期：1979-08-01 00:00:00
abstract：PURPOSE:To describe a case of severe dry eye syndrome in a child. METHODS:Observational case report. The authors describe a 10-year-old male with severe dry eyes who was profoundly disabled by pain and photophobia despite aggressive conventional therapy. Lacrimal gland histology was consistent with the primary Sjögren...
journal_title：American journal of ophthalmology
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更新日期：2001-12-01 00:00:00
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journal_title：American journal of ophthalmology
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更新日期：1979-07-01 00:00:00
abstract：PURPOSE:To assess the risks of vitrectomy for the removal of primary and secondary vitreous opacities. DESIGN:Retrospective, nonrandomized, interventional case series. METHODS:We reviewed the results of 116 consecutive cases of vitrectomy for vitreous floaters. Eighty-six cases were primary and 30 cases were secondar...
journal_title：American journal of ophthalmology
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更新日期：2011-06-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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abstract：PURPOSE:To evaluate the relationship between rates of change on frequency doubling technology (FDT) perimetry and longitudinal changes in quality of life (QoL) of glaucoma patients. DESIGN:Prospective observational cohort study. METHODS:One hundred fifty-two subjects (127 glaucoma and 25 healthy) were followed for an...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2015.04.007
更新日期：2015-07-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.ajo.2008.01.022
更新日期：2008-06-01 00:00:00
abstract：PURPOSE/METHODS:A 74-year-old woman had multiple tumors including a retinal mass. Her medical history included cutaneous sebaceous adenomas, adenocarcinoma of the breast, and colonic adenocarcinoma. Her involved eye became painful and was enucleated. RESULTS/CONCLUSIONS:Examination of the enucleated eye disclosed meta...
journal_title：American journal of ophthalmology
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更新日期：1995-08-01 00:00:00
abstract：PURPOSE:To determine the regional differences in the test-retest variability of topographic measurements of the optic nerve head obtained with a confocal scanning laser retina tomograph. METHODS:Three 10-degree topographic images centered on the optic disk of 20 individuals (20 eyes), five normal subjects, seven glauc...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(14)72656-x
更新日期：1995-10-01 00:00:00
abstract：PURPOSE:To identify clinical and microbiologic factors influencing the prevalence and visual outcomes in endophthalmitis after deadly-weapon-related open-globe injuries. METHODS:In a retrospective study of 228 eyes of 212 patients with deadly-weapon-related open-globe injuries, clinical and microbiologic factors influ...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(01)01320-4
更新日期：2002-01-01 00:00:00
abstract：PURPOSE:This clinical case report demonstrates blepharoptosis-induced corneal steepening and its subsequent resolution after blepharoptosis surgery. METHODS:A 62-year-old man complaining of blurred vision without apparent cause on clinical examination underwent keratometry and computerized corneal topography, which re...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
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更新日期：2000-08-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章,多中心研究
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(75)90075-6
更新日期：1975-02-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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更新日期：2007-09-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章,多中心研究,随机对照试验
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更新日期：2010-04-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2010.03.024
更新日期：2010-09-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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更新日期：1975-02-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(01)01179-5
更新日期：2001-11-01 00:00:00
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journal_title：American journal of ophthalmology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.ajo.2019.05.030
更新日期：2019-11-01 00:00:00
abstract：PURPOSE:To compare the longitudinal vault changes after implantation of a posterior chamber phakic intraocular lens (pIOL) (Visian implantable contact lens) with (ICL V4c) and without (ICL V4) a central artificial hole for moderate to high myopia in Chinese eyes. DESIGN:Retrospective case series. METHODS:This study c...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2016.09.016
更新日期：2016-12-01 00:00:00
abstract：:Because determination of the differential light threshold of a visual field is a psychophysiologic test, the results can be subject to variation. This scatter measured during a single examination is usually called the short-term fluctuation. Increases of the short-term fluctuation were thought to occur primarily in ab...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(89)90667-3
更新日期：1989-04-15 00:00:00
abstract：PURPOSE:To update the biomicroscopic classification and anatomic interpretations of the stages of development of age-related macular hole and provide explanations for the remarkable recovery of visual acuity that occurs in some patients after vitreous surgery. METHODS:Recent biomicroscopic observations of various stag...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(14)72781-3
更新日期：1995-06-01 00:00:00
