Respiratory syncytial virus-induced cytokine production by neonatal macrophages.

Abstract:

:The induction of immunoregulatory cytokines IL-1 beta, IL-6, IL-12, tumour necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) was studied with neonatal (cord blood) monocyte-derived macrophages (MDM) after in vitro infection with respiratory syncytial virus (RSV). The expression of mRNAs for these cytokines in RSV-infected MDM was examined by reverse transcriptase-polymerase chain reaction (RT-PCR). The activities of these cytokines were assayed by ELISA. Significant increase of expression of mRNA for IL-6, IL-12, TNF-alpha and IFN-gamma occurred within 2 h after infection and decreased within 6 h after infection. At 20 h after infection the MDM produced and secreted moderate levels of IL-6 and TNF-alpha; however, no IL-12 and IFN-gamma activities were detected. Moderate IL-1 beta mRNA was expressed before RSV infection, and its expression increased at 2 h after infection. However, no detectable IL-1 beta was secreted in culture fluids. These observations suggest that RSV-infected neonatal macrophages produce and secrete IL-6 and TNF-alpha quickly during the eclipse phase of RSV infection and therefore may play a prominent role in the initiation of the immune response to RSV.

journal_name

Clin Exp Immunol

authors

Tsutsumi H,Matsuda K,Sone S,Takeuchi R,Chiba S

doi

10.1046/j.1365-2249.1996.d01-874.x

subject

Has Abstract

pub_date

1996-12-01 00:00:00

pages

442-6

issue

3

eissn

0009-9104

issn

1365-2249

journal_volume

106

pub_type

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