Abstract:
:Dexras1, a newly identified member of the Ras superfamily of proteins, was discovered in AtT-20 corticotrope cells because its expression was induced in response to glucocorticoids (dexamethasone; Dex). As yet, the function of Dexras1 is unknown, but its rapid induction in response to glucocorticoids suggests the possibility that it may be involved in negative feedback regulation of corticotropin secretion. To better understand the control of Dexras1 expression, possible effects of other steroid hormones on its expression were studied in both AtT-20 cells and in mouse pituitaries. AtT-20 cells were treated with each of 6 steroids [aldosterone, corticosterone (Cort), Dex, beta-estradiol (E(2)), progesterone and testosterone] for 2 h. Dexras1 expression was assessed using both reverse transcription polymerase chain reaction (RT-PCR) and Northern analysis. Expression of the gene was only induced in response to glucocorticoid treatment (Dex or Cort). The 6 steroids were also injected into mice, pituitaries were harvested and total RNA was obtained for RT-PCR analysis. Surprisingly, treatment with E(2), not only injection of glucocorticoids, induced Dexras1 expression in mouse pituitary. Other steroids were without effect. The results suggest that in AtT-20 corticotropes, Dexras1 expression is only induced by glucocorticoid-type steroids. In pituitary glands of mice, the gene's expression is also responsive to E(2). We conclude that either Dexras1 expression in corticotropes from normal mice is regulated differently from that in AtT-20 cells, or that Dexras1 is also expressed in other pituitary cells than corticotropes.
journal_name
Neuroendocrinologyjournal_title
Neuroendocrinologyauthors
Brogan MD,Behrend EN,Kemppainen RJdoi
10.1159/000054691subject
Has Abstractpub_date
2001-10-01 00:00:00pages
244-50issue
4eissn
0028-3835issn
1423-0194pii
54691journal_volume
74pub_type
杂志文章abstract::The capability of the anterior pituitary gland to secrete growth hormone (GH) in response to an intravenous injection of growth hormone-releasing factor, hpGRF-44, was evaluated in male rats ranging from 22 days to 24 months of age. The increase in GH concentrations following a submaximal dose of hpGRF-44 (0.5 microgr...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123594
更新日期:1983-12-01 00:00:00
abstract::The incidence of obesity is rapidly escalating and has reached epidemic proportions. In all species, including rodents, humans, and sheep, there is large variation in the degree of weight gain across individuals in response to an obesogenic environment. This individual variation is, at least in part, determined by inn...
journal_title:Neuroendocrinology
pub_type: 杂志文章,评审
doi:10.1159/000455232
更新日期:2017-01-01 00:00:00
abstract::Recent evidence suggests that the free amino-terminal fragment of procalcitonin (N-PCT) plays a role in the central control of feeding behavior and energy homeostasis. However, little is known about the mechanisms through which N-PCT works. Here we report that intracerebroventricular administration of N-PCT to free-fe...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000155137
更新日期:2008-01-01 00:00:00
abstract::Pit-1 expression has been reported to be cell-type-specific in the adult human pituitary and in human pituitary adenomas. In contrast, studies of rodent fetal adenohypophysial development as well as mature rodent glands have indicated that the pit-1 mRNA is ubiquitously expressed and the protein is under translational...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126975
更新日期:1996-04-01 00:00:00
abstract::Immunoreactive thyroid-stimulating hormone (IR-TSH) has been detected in the hypothalamus and is released in vitro by a calcium-dependent mechanism when the tissue is depolarized. Recently, immunocytochemical studies have revealed that IR-TSH is present in thyrotropes in the pars tuberalis. Therefore, because these th...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124149
更新日期:1985-07-01 00:00:00
abstract::Sexual behavior and elevation of gonadal steroids were induced in both male and female New Zealand white rabbits following injection of synthetic beta (1-24) ACTH into the lateral cerebral ventricle. In blood assays collected by cardiac puncture, testosterone, progesterone and corticosterone were all increased above b...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000122441
更新日期:1975-01-01 00:00:00
abstract::The effects of adrenalectomy (ADX) and dexamethasone (DEX) treatment on brain dopamine (DA) receptors of ovariectomized (OVX) rats were investigated by autoradiography using binding of the D1 and D2 antagonists ligands [3H]SCH 23390 and [3H]spiperone, respectively. Fourteen days after ADX, D1 receptors decreased in th...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126158
更新日期:1992-04-01 00:00:00
abstract::In addition to its traditional role as a circulating vasoactive peptide, vasopressin (VP) has been shown to play significant roles in central cardiovascular processing. The recent description of VP receptors within the subfornical organ (SFO) has suggested this circumventricular organ (CVO) as a potential locus for fe...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000127230
更新日期:1997-08-01 00:00:00
abstract::The role of endogenous corticoids in fever responses caused by recombinant murine interleukin (IL)-1 beta and IL-6 was studied in adult male Wistar rats. Adrenalectomy diminished the development of fever after intracerebroventricular (icv) injection of these ILs and lowered body temperature. Intraperitoneal administra...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126682
更新日期:1994-04-01 00:00:00
abstract::The neurotransmitter and andrenoceptors involved in the inhibition of luteinizing hormone (LH) release induced by stimulating the locus ceruleus nucleus (LC) have been characterized in ovariectomized, estrogen-primed rats in which the release of LH was triggered by electrical stimulation of the medial preoptic area. B...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123858
更新日期:1984-01-01 00:00:00
abstract::Bromocriptine or other dopamine agonists are usually effective for the treatment of prolactin-secreting adenomas. Five to 18% of prolactinomas, however, do not respond to such therapy. We have shown previously that such resistance to bromocriptine correlates with reduced binding to the D2 receptor subtype of dopamine,...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126764
更新日期:1994-09-01 00:00:00
abstract::Pituitary adenylate cyclase-activating polypeptide (PACAP) receptors were characterized and their function investigated in mouse pituitary neurointermediate lobe explants. We show that mouse neurointermediate lobes can be maintained for up to 1 month in defined medium. After 8 days in culture, these explants are devoi...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000127091
更新日期:1996-07-01 00:00:00
abstract::Previous studies have shown that the hypothalamic concentration of pro-opiomelanocortin (POMC) mRNA and several POMC-derived peptides increases in the rat 4 weeks after orchiectomy and that this increase can be prevented by testosterone replacement. In this study, we have examined the short-term effects of orchiectomy...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000125638
更新日期:1990-11-01 00:00:00
abstract::VIP stimulated prolactin secretion from incubated rat hemipituitaries. Under the same conditions, the secretion of GH, LH, FSH was not affected. The stimulation of prolactin was dose-dependent, with an apparent affinity of VIP of 10.9 +/- 3.1 nM and a maximal stimulation of 57.7 +/- 4.2%. Secretin, a structurally rela...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123074
更新日期:1980-09-01 00:00:00
abstract::High affinity binding of 3H-flunitrazepam (FNZP) to crude membrane preparations of bovine pineal membranes was examined by a rapid filtration procedure through Whatman GFB paper. At 0 degrees C binding reached equilibrium in about 20 min. Scatchard analysis of data at equilibrium revealed a single population of bindin...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123533
更新日期:1983-08-01 00:00:00
abstract:BACKGROUND:The outcomes of bariatric surgery are very irregular and mostly unpredictable. The search for variables of predictive value is encouraged to help preventing therapeutic failures. OBJECTIVE:We aimed to confirm the hypothesis that preexisting eating behaviors could predict neuroendocrine and metabolic outcome...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000500687
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Manipulating thyroid hormones has been shown to influence learning and memory. Although a large body of literature is available on the effects of thyroid hormone deficiency on learning and memory functions during developmental or adult-onset hypothyroidism, electrophysiological findings are limited. This lim...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000328513
更新日期:2011-01-01 00:00:00
abstract::A significant increase of cell multiplication in inoculated ascitic and solid tumors was demonstrated in both DBA/2 and C57BL/6 mice as well as in Wistar rats after radiofrequency lesions in the median hypothalamus (ventromedial and dorsomedial nuclei; part of arcuate nucleus). The following tests were performed: mito...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124479
更新日期:1986-01-01 00:00:00
abstract::Growth hormone-releasing hormone (GHRH) stimulates GH secretion in man and the hormonal response is specific. The attenuation of GH response to bolus GHRH after prior exposure of GHRH of up to 24 h was not demonstrated in normal or GH-deficient subjects after more prolonged exposure. This suggests that the partial los...
journal_title:Neuroendocrinology
pub_type: 杂志文章,评审
doi:10.1159/000125793
更新日期:1991-01-01 00:00:00
abstract::Blood samples were removed via chronic intra-atrial cannulae every 15 min in female rats during the estrous cycle, the last week of pregnancy, parturition and suckling. Growth hormone (GH) secretion during the estrous cycle is characterized by episodic release, occurring approximately once hourly. The surges in GH inc...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000122525
更新日期:1976-01-01 00:00:00
abstract::Dehydration is a classic homeostatic stressor in rats that leads to a series of endocrine responses including stimulation of the hypothalamo-pituitary-adrenal (HPA) axis. During the last decade, it has been well established that perinatal food restriction is associated with the onset of diseases in adults. Our previou...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000078098
更新日期:2004-01-01 00:00:00
abstract::Pituitary oxytocin (OT) secretion is inversely related to saline consumption in several experimental models of sodium appetite in rats. Because systemic OT administration does not inhibit sodium appetite, release of OT as a neurotransmitter within the brain, coincident with its secretion from the pituitary, may be rel...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126236
更新日期:1992-08-01 00:00:00
abstract::The sleep-wakefulness pattern and brain protein levels were determined in rats for 3 h following these conditions: administration of an inhibitor of protein synthesis (anisomycin), administration of several doses of rat growth hormone (GH) or thyrotropin, and administration of a combination of anisomycin + GH or thyro...
journal_title:Neuroendocrinology
pub_type: 杂志文章,评审
doi:10.1159/000122377
更新日期:1975-01-01 00:00:00
abstract::The hypothalamic GnRH pulse generator activity, which was assessed by the characteristic increases in the multiple unit activity (MUA) specifically associated with the pulsatile LH secretion (MUA volleys), was monitored throughout the estrous cycle of the female goat. Blood samples were collected daily for analysis of...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000127051
更新日期:1995-12-01 00:00:00
abstract::Arginine-vasopressin (AVP) is regarded as a potent stimulator of pituitary adrenocorticotropin (ACTH) secretion and participates therefore in the regulation of the hypothalamic-pituitary-adrenal (HPA) axis function in concert with the physiological activator of the axis, hypothalamic corticotropin-releasing hormone (C...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126787
更新日期:1994-11-01 00:00:00
abstract::The recent description of infertility in humans with loss-of-function mutations in genes for neurokinin B (NKB) or its receptor (NK3R) has focused attention on the importance of this tachykinin in the control of GnRH secretion. In a number of species, NKB neurons in the arcuate nucleus also produce two other neuropept...
journal_title:Neuroendocrinology
pub_type: 杂志文章,评审
doi:10.1159/000355285
更新日期:2014-01-01 00:00:00
abstract::The growth hormone secretagogue receptor subtype 1a (GHSR-1a) is involved in biological actions of ghrelin by triggering intracellular second messengers coupled to heterotrimeric G-protein complex involving Galpha(q/11). Adenosine is a partial agonist of the GHSR-1a, binding to a binding pocket distinct from the one d...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000076042
更新日期:2004-01-01 00:00:00
abstract::A patch-clamp study was conducted on cultured frog pituitary melanotrophs, in order to investigate the effects of adrenaline on the electrical activity of these cells. In the whole-cell configuration, adrenaline (1 microM) caused hyperpolarization that was accompanied by a fall in membrane input resistance and a block...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126633
更新日期:1994-01-01 00:00:00
abstract::Structural and functional impairment of the skeletal system remains an important cause of morbidity and disability in patients with Cushing's syndrome (CS). Glucocorticoid (GC) excess inhibits bone formation and calcium absorption from the gut, increases bone resorption, and alters the secretion of gonadotropin and gr...
journal_title:Neuroendocrinology
pub_type: 杂志文章,评审
doi:10.1159/000314298
更新日期:2010-01-01 00:00:00
abstract::Adenylate cyclase activity was studied in anterior pituitary homogenates from young adult (6 months) and old (20 and 24 months) male rats. Basal, NaF-, GTP-, 5'-guanylimidodiphosphate (Gpp(NH)p)- and forskolin-stimulated activities were similar in the three groups, implying that the stimulatory guanyl nucleotide regul...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124682
更新日期:1986-01-01 00:00:00