Adrenaline induces hyperpolarization in frog pituitary melanotrophs through activation of potassium channels.

Abstract:

:A patch-clamp study was conducted on cultured frog pituitary melanotrophs, in order to investigate the effects of adrenaline on the electrical activity of these cells. In the whole-cell configuration, adrenaline (1 microM) caused hyperpolarization that was accompanied by a fall in membrane input resistance and a blockage of spontaneous action potentials. Under voltage clamp, adrenaline elicited a net-outward current. The hyperpolarization became undetectable at a command voltage of -100 mV which corresponded to the equilibrium potential of potassium ions. The effect of adrenaline on membrane potential and spontaneous activity was blocked by the alpha 2-adrenergic receptor antagonist yohimbine (1-10 microM) but could not be mimicked by the alpha 2-adrenergic agonist clonidine (1-10 microM). In the cell-attached configuration, exposure of the extra-patch membrane to adrenaline increased the occurrence of single-channel currents with a slope conductance of 100 pS. The deduced reversal potential of these currents corresponded to the equilibrium potential of potassium ions. These results suggest that frog melanotrophs display an alpha 2-adrenergic receptor subtype coupled to potassium channels involved in hyperpolarization.

journal_name

Neuroendocrinology

journal_title

Neuroendocrinology

authors

Valentijn JA,Vaudry H,Cazin L

doi

10.1159/000126633

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

20-8

issue

1

eissn

0028-3835

issn

1423-0194

journal_volume

59

pub_type

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