A new model for random screening inhibitors of vascular endothelial growth factor receptor 1 kinase.

abstract：AIM:To identify the genetic variants of angiotensin II type 2 receptor (AT2R) gene in a Chinese population and to determine whether the AT2R gene polymorphisms are associated with essential hypertension (EH). METHODS:The detection of single nucleotide polymorphisms (SNPs) was performed in 19 subjects by a direct DNA s...

journal_title：Acta pharmacologica Sinica

authors： Zhang Y,Zhang KX,Wang GL,Huang W,Zhu DL

更新日期：2003-11-01 00:00:00

abstract：AIM:To study and establish a proteome reference map and regulation network of neonatal rat cardiomyocyte. METHODS:Cultured cardiomyocytes of neonatal rats were used. All proteins expressed in the cardiomyocytes were separated and identified by two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assist...

journal_title：Acta pharmacologica Sinica

authors： Li ZJ,Liu N,Han QD,Zhang YY

更新日期：2011-09-01 00:00:00

abstract：AIM:The metabolites of naproxen produced by Cunninghamella species were isolated and identified, and further to compare the similarities between microbial transformation and mammalian metabolism. METHODS:Naproxen was transformed by three strains of Cunninghammella species (Cunninghamella blakeslesna AS 3.153, Cunningh...

journal_title：Acta pharmacologica Sinica

authors： Zhong DF,Sun L,Liu L,Huang HH

更新日期：2003-05-01 00:00:00

abstract：AIM:To investigate the mechanism of action of a potent analgesic, (+/-)-meptazinol. METHODS:The structures of meptazinol enantiomers were compared with opioid pharmacophore and tramadol. RESULTS:Neither enantiomer of meptazinol fitted any patterns among the opioid pharmacophore and tramadol, although they did share s...

journal_title：Acta pharmacologica Sinica

authors： Li W,Hao JL,Tang Y,Chen Y,Qiu ZB

更新日期：2005-03-01 00:00:00

abstract：AIM:To investigate modulation of antagonist and agonist binding to adenosine A1 receptors by MgCl2 and 5 -guanylimidodiphosphate (Gpp(NH)p) using rat brain membranes and the A1 antagonist [3H]-8-cyclopentyl-1,3-dipropylxanthine ([3H]DPCPX) and the A1 agonist [3H]-2-chloro-N6-cyclopentyladenosine ([3H]CCPA). METHODS:Pa...

journal_title：Acta pharmacologica Sinica

authors： Finlayson K,Maemoto T,Butcher SP,Sharkey J,Olverman HJ

更新日期：2003-08-01 00:00:00

abstract：:Central nervous system (CNS) disorders represent a broad spectrum of brain ailments with short- and long-term disabilities, and nanomedicine-based approaches provide a new therapeutic approach to treating CNS disorders. A variety of potential drugs have been discovered to treat several neuronal disorders; however, the...

journal_title：Acta pharmacologica Sinica

authors： Hanif S,Muhammad P,Chesworth R,Rehman FU,Qian RJ,Zheng M,Shi BY

更新日期：2020-07-01 00:00:00

abstract：:The chemokine receptor CCR5 is an important anti-HIV (human immunodeficiency virus) drug target owning to its pivotal role in HIV-1 viral entry as a co-receptor. Here, we present a 2.9 Å resolution crystal structure of CCR5 bound to PF-232798, a second-generation oral CCR5 antagonist currently in phase II clinical tri...

journal_title：Acta pharmacologica Sinica

authors： Zhu Y,Zhao YL,Li J,Liu H,Zhao Q,Wu BL,Yang ZL

更新日期：2019-04-01 00:00:00

abstract：AIM:To examine whether the cell growth inhibitory effect of the combination of baicalin and baicalein is related to apoptosis. Moreover, to determine whether the expression of some apoptosis-related proteins is regulated by the ERK/p38 MAPK pathway. METHODS:Cell viability was measured using a 3-(4,5-dimethylthiazol-2-...

journal_title：Acta pharmacologica Sinica

authors： Zhou QM,Wang S,Zhang H,Lu YY,Wang XF,Motoo Y,Su SB

更新日期：2009-12-01 00:00:00

abstract：:Specific chromosome aberrations are frequently detected during the development of hepatocellular carcinoma. Molecular cytogenetic approaches such as comparative genomic hybridization and loss of heterozygosity analyses have provided fruitful information on changes in HCC cases at the genomic level. Mapping of chromoso...

journal_title：Acta pharmacologica Sinica

authors： Lau SH,Guan XY

更新日期：2005-06-01 00:00:00

abstract：AIM:Fibroblast growth factor 7 (FGF7) is involved in a number of physiological and pathological processes, including lung disease. However, relatively little is known about the effect of FGF7 gene polymorphisms on chronic obstructive pulmonary disease (COPD) susceptibility. This study aimed to investigate the associati...

journal_title：Acta pharmacologica Sinica

authors： Xu SC,Kuang JY,Liu J,Ma CL,Feng YL,Su ZG

更新日期：2012-08-01 00:00:00

abstract：:Cardiovascular diseases (CVD) are the leading cause of morbidity and mortality in the world. Although considerable progress has been made in the diagnosis, treatment and prognosis of CVD, there is still a critical need for novel diagnostic biomarkers and new therapeutic interventions to decrease the incidence of this ...

journal_title：Acta pharmacologica Sinica

authors： Zhou SS,Jin JP,Wang JQ,Zhang ZG,Freedman JH,Zheng Y,Cai L

更新日期：2018-07-01 00:00:00

abstract：AIM:To determine the active ingredient of Niuchangchih (Antrodia camphorata) responsible for its anti-inflammatory effects and the relevant molecular mechanisms. METHODS:Five major antcins (A, B, C, H, and K) were isolated from fruiting bodies of Niuchangchih. Structural similarity between the antcins and 2 glucocorti...

journal_title：Acta pharmacologica Sinica

authors： Chen YC,Liu YL,Li FY,Chang CI,Wang SY,Lee KY,Li SL,Chen YP,Jinn TR,Tzen JT

更新日期：2011-07-01 00:00:00

abstract：:It was reported that antituberculosis medicines could induce liver damage via oxidative stress. In this study, we investigated the effects of rifampicin (RFP) on the membrane expression of multidrug resistance-associated protein 2 (MRP2) and the relationship between oxidative stress and RFP-induced endocytosis of MRP2...

journal_title：Acta pharmacologica Sinica

authors： Xu BY,Tang XD,Chen J,Wu HB,Chen WS,Chen L

更新日期：2020-01-01 00:00:00

abstract：AIM:To investigate the effects of onychin on the proliferation of cultured rat artery vascular smooth muscle cells (VSMCs) in the presence of 10% new-born calf serum (NCS). METHODS:Rat VSMCs were incubated with onychin 150 micromol/L or genistein 10 micromol/L in the presence of 10% NCS for 24 h. The proliferation of ...

journal_title：Acta pharmacologica Sinica

authors： Yang M,Huang HL,Zhu BY,Tuo QH,Liao DF

更新日期：2005-02-01 00:00:00

abstract：AIM:To study the changes of phospholipase D (PLD) activity of actively sensitized rat peritoneal mast cells (RPMC) in degranulation. METHODS:Degranulation of RPMC was determined by measurement of beta-hexosaminidase release. PLD activity assay was carried out by measurement of PLD product, choline, with chemiluminesce...

journal_title：Acta pharmacologica Sinica

authors： Lu YB,Wu M,Zhou HL

更新日期：2004-01-01 00:00:00

abstract：AIM:To study wound healing activity of the human placental extract (HPE) in rats. METHODS:Full thickness wounds were inflicted on depilated dorsum of Charles foster rats with 8 mm Acu-punch biopsy. The HPE was applied both at topical and im routes (2.5 mL/kg). Effects were compared on the basis of physical criteria, b...

journal_title：Acta pharmacologica Sinica

authors： Biswas TK,Auddy B,Bhattacharya NP,Bhattacharya S,Mukherjee B

更新日期：2001-12-01 00:00:00

abstract：:Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as neural apoptosis regulated convertase (NARC1), is a key modulator of cholesterol metabolism. PCSK9 increases the serum concentration of low-density lipoprotein cholesterol by escorting low-density lipoprotein receptors (LDLRs) from the membrane of he...

journal_title：Acta pharmacologica Sinica

authors： He NY,Li Q,Wu CY,Ren Z,Gao Y,Pan LH,Wang MM,Wen HY,Jiang ZS,Tang ZH,Liu LS

更新日期：2017-03-01 00:00:00

abstract：AIM:To test the hypothesis that PI3K/Akt/eNOS signaling has a protective role in a murine model of ventilation associated lung injury (VALI) through down-regulation of p38 MAPK signaling. METHODS:Male C57BL/J6 (wild-type, WT) or eNOS knockout mice (eNOS(-/-)) were exposed to mechanical ventilation (MV) with low (LV(T)...

journal_title：Acta pharmacologica Sinica

authors： Peng XQ,Damarla M,Skirball J,Nonas S,Wang XY,Han EJ,Hasan EJ,Cao X,Boueiz A,Damico R,Tuder RM,Sciuto AM,Anderson DR,Garcia JG,Kass DA,Hassoun PM,Zhang JT

更新日期：2010-02-01 00:00:00

abstract：AIM:We have shown that a combination of ligustrazine and berberine produces more effective inhibition on platelet activation and inflammatory reactions in rat acute myocardial infarction compared with either agent alone. In this study we evaluated the beneficial effects of a combination of ligustrazine and berberine in...

journal_title：Acta pharmacologica Sinica

authors： Zhang Y,Ma XJ,Guo CY,Wang MM,Kou N,Qu H,Mao HM,Shi DZ

更新日期：2016-04-01 00:00:00

abstract：AIM:To explore the action mechanism of antidepressants. METHODS:The PC12 cell proliferation was detected by flow cytometry. The proliferation of hippocampal progenitor cells and level of brain-derived neurotrophic factor (BDNF) were measured by immunohistochemistry. RESULTS:Treatment with N-methylaspartate (NMDA) 600...

journal_title：Acta pharmacologica Sinica

authors： Li YF,Zhang YZ,Liu YQ,Wang HL,Yuan L,Luo ZP

更新日期：2004-11-01 00:00:00

abstract：:Korean Panax ginseng C A Meyer is mainly used to maintain the homeostasis of the body, and the pharmacological efficacy of Korean ginseng identified by modern science includes improved brain function, pain-relieving effects, preventive effects against tumors as well as anti-tumor activity, enhanced immune system funct...

journal_title：Acta pharmacologica Sinica

authors： Choi KT

更新日期：2008-09-01 00:00:00

abstract：AIM:To study the expressions of vascular endothelial growth factor (VEG F), angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2), Tie-1, and Tie-2 in C57BL/6 mouse brain after permanent focal cerebral ischemia. METHODS:The mRNA levels of VEGF, Ang-1, Ang-2, Tie-1, and Tie-2 were measured by semiquantitative reverse transcri...

journal_title：Acta pharmacologica Sinica

authors： Wang RG,Zhu XZ

更新日期：2002-05-01 00:00:00

abstract：AIM:In order to elucidate the molecular mechanism underlying the cardioprotection afforded by schisandrin B (Sch B), the effect of Sch B treatment on the sensitivity of mitochondria to Ca2+-stimulated permeability transition (PT) was investigated in rat hearts under normal and ischemia-reperfusion (I-R) conditions. RE...

journal_title：Acta pharmacologica Sinica

authors： Chiu PY,Leung HY,Siu AH,Poon MK,Ko KM

更新日期：2007-10-01 00:00:00

abstract：AIM:To investigate the mechanism of brain interleukin-1 beta (IL-1 beta) in reserpine-induced behavioral depression in rats. METHODS:Porsult swim test was used in the measurement of depressive behavior and ELISA was used in measurement of brain IL-1 beta. RESULTS:Intraperitoneal injection of reserpine (0, 4, 6, and 8...

journal_title：Acta pharmacologica Sinica

authors： Huang QJ,Jiang H,Hao XL,Minor TR

更新日期：2004-03-01 00:00:00

abstract：AIM:To develop a robust, cell-based, high-content screening (HCS) assay based on receptor internalization for the identification of novel modulators of the epidermal growth factor receptor (EGFR). METHODS:Agonist-induced receptor internalization is part of the signaling cascade of EGFR. Fluorescent-tagged epidermal gr...

journal_title：Acta pharmacologica Sinica

authors： Wang J,Xie X

更新日期：2007-10-01 00:00:00

abstract：:Autophagy is a cell self-digestion process via lysosomes that clears "cellular waste", including aberrantly modified proteins or protein aggregates and damaged organelles. Therefore, autophagy is considered a protein and organelle quality control mechanism that maintains normal cellular homeostasis. Dysfunctional auto...

journal_title：Acta pharmacologica Sinica

authors： He LQ,Lu JH,Yue ZY

更新日期：2013-05-01 00:00:00

abstract：:Cardiac amyloidosis (CA) comprises a heterogeneous group of medical conditions affecting the myocardium. It presents with proteinaceous infiltration with variable degrees of severity, prevalence and evolution. Despite this heterogeneity, erroneous protein folding is the common pathophysiologic process, yielding the fo...

journal_title：Acta pharmacologica Sinica

authors： Luciani M,Troncone L,Monte FD

更新日期：2018-07-01 00:00:00

abstract：AIM:To investigate the ability of an antisense RNA eukaryotic expression plasmid pcDNA3.1/survivin in down-regulating the expression level of survivin mRNA and survivin protein and reversed multidrug resistance (MDR) in adriamycin-resistant HL-60/ADR cell line. METHODS:The expression of survivin mRNA was measured by R...

journal_title：Acta pharmacologica Sinica

authors： Wang L,Zhang GM,Feng ZH

更新日期：2003-12-01 00:00:00

abstract：:Aspirin (ASA) is a cardioprotective drug with anti-cardiac fibrosis action in vivo. This study was aimed to clarify the anti-cardiac fibrosis action of ASA and the underlying mechanisms. Two heart injury models (injection of isoproterenol and ligation of the left anterior descending branch) were used in mice to induce...

journal_title：Acta pharmacologica Sinica

authors： Liu PP,Liu HH,Sun SH,Shi XX,Yang WC,Su GH,Zhao J

更新日期：2017-04-01 00:00:00

abstract：AIM:G protein-coupled inwardly rectifying potassium channels (GIRK) are important for neuronal signaling and membrane excitability. In the present study, we intend to find whether GIRK channels express functionally in adult rat dorsal root ganglion (DRG) neurons. METHODS:We used RT-PCR to detect mRNA for 4 subunits of...

journal_title：Acta pharmacologica Sinica

authors： Gao XF,Zhang HL,You ZD,Lu CL,He C

更新日期：2007-02-01 00:00:00