Comparison of effects of MgCl2 and Gpp(NH)p on antagonist and agonist radioligand binding to adenosine A1 receptors.

abstract：AIM:To examine the in vitro antitumor activity of resveratrol against multiple myeloma (MM) cell lines (RPMI 8226, U266, and KM3), and the mechanisms involved. METHODS:The growth inhibition of resveratrol was determined by 3-(4, 5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The effect of resve...

journal_title：Acta pharmacologica Sinica

authors： Sun CY,Hu Y,Guo T,Wang HF,Zhang XP,He WJ,Tan H

更新日期：2006-11-01 00:00:00

abstract：:An early intervention using biomarkers to predict acute myocardial infarction (AMI) will effectively reduce global heart attack incidence, particularly among high-risk patients with type 2 diabetes mellitus (T2DM). This study attempted to identify potential biomarkers by detecting changes in the levels of plasma prote...

journal_title：Acta pharmacologica Sinica

authors： Rahim MAA,Rahim ZHA,Ahmad WAW,Bakri MM,Ismail MD,Hashim OH

更新日期：2018-07-01 00:00:00

abstract：:Over half of patients with BRCA1-deficient cancers do not respond to treatment with poly(ADP-ribose) polymerase (PARP) inhibitors. In this study, we report that a combination of 53BP1 and BRCA1 may serve as a biomarker of PARP inhibitor sensitivity. Based on the mRNA levels of four homologous recombination repair (HR)...

journal_title：Acta pharmacologica Sinica

authors： Yang ZM,Liao XM,Chen Y,Shen YY,Yang XY,Su Y,Sun YM,Gao YL,Ding J,Zhang A,He JX,Miao ZH

更新日期：2017-07-01 00:00:00

abstract：AIM:Hydrogen peroxide (H2O2) is produced during liver transplantation. Ischemia/reperfusion induces oxidation and causes intracellular Ca2+ overload, which harms liver cells. Our goal was to determine the precise mechanisms of these processes. METHODS:Hepatocytes were extracted from rats. Intracellular Ca2+ concentrat...

journal_title：Acta pharmacologica Sinica

authors： Sato H,Takeo T,Liu Q,Nakano K,Osanai T,Suga S,Wakui M,Wu J

更新日期：2009-01-01 00:00:00

abstract：AIM:To study the effects of tanshinone IIA (TIIA) on lipopolysaccharide (LPS)-induced acute lung injury in mice and the underlying mechanisms. METHODS:Mice were injected with LPS (10 mg/kg, i.p.), then treated with TIIA (10 mg/kg, i.p.). Seven hours after LPS injection, the lungs were collected for histological study....

journal_title：Acta pharmacologica Sinica

authors： Xu M,Cao FL,Zhang YF,Shan L,Jiang XL,An XJ,Xu W,Liu XZ,Wang XY

更新日期：2015-02-01 00:00:00

abstract：AIM:To isolate the G4 and G1 molecular forms of acetylcholinesterase in rat cortex for interpreting the therapeutical effect of the AChE inhibitor drugs. METHODS:Size exclusion chromatography and ultracentrifugation were used to isolate and ascertain the G4 and G1 molecular forms of AChE. RESULTS:After size exclusion...

journal_title：Acta pharmacologica Sinica

authors： Zhao Q,Tang XC

更新日期：2002-02-01 00:00:00

abstract：AIM:To Study the interaction of macrophage colony-stimulating factor (M-CSF) and interleukin-10 (IL-10) in productions of IL-12 and IL-18 and expressions of CD14, CD23, and CD64 by human monocytes. METHODS:Purified adherent human monocytes were cultured with M-CSF or IL-10 alone, or with M-CSF+IL-10 and 2-3d later, th...

journal_title：Acta pharmacologica Sinica

authors： Ji XH,Yao T,Qin JC,Wang SK,Wang HJ,Yao K

更新日期：2004-10-01 00:00:00

abstract：AIM:To investigate whether nimesulide could suppress tumor growth and induce apoptosis in implanted hepatoma mice and to explore the molecular mechanisms. METHODS:Male mice received nimesulide 10 mg/kg, 20 mg/kg, and 40 mg/kg ig daily for 21 d. Electron microscopy (EM), flow cytometry (FCM), DNA ladder, radioimmunoass...

journal_title：Acta pharmacologica Sinica

authors： Li XH,Li JJ,Zhang HW,Sun P,Zhang YL,Cai SH,Ren XD

更新日期：2003-10-01 00:00:00

abstract：AIM:Abrus agglutinin (AGG) from the seeds of Indian medicinal plant Abrus precatorius belongs to the class II ribosome inactivating protein family. In this study we investigated the anticancer effects of AGG against human hepatocellular carcinoma in vitro and in vivo. METHODS:Cell proliferation, DNA fragmentation, Ann...

journal_title：Acta pharmacologica Sinica

authors： Mukhopadhyay S,Panda PK,Das DN,Sinha N,Behera B,Maiti TK,Bhutia SK

更新日期：2014-06-01 00:00:00

abstract：:The hypnotic agent propofol is effective for the induction and maintenance of anesthesia. However, recent studies have shown that propofol administration is related to arrhythmias. Propofol displays both pro- and anti-arrhythmic effects in a concentration-dependent manner. Data indicate that propofol can convert supra...

journal_title：Acta pharmacologica Sinica

authors： Liu Q,Kong AL,Chen R,Qian C,Liu SW,Sun BG,Wang LX,Song LS,Hong J

更新日期：2011-06-01 00:00:00

abstract：AIM:To determine the active ingredient of Niuchangchih (Antrodia camphorata) responsible for its anti-inflammatory effects and the relevant molecular mechanisms. METHODS:Five major antcins (A, B, C, H, and K) were isolated from fruiting bodies of Niuchangchih. Structural similarity between the antcins and 2 glucocorti...

journal_title：Acta pharmacologica Sinica

authors： Chen YC,Liu YL,Li FY,Chang CI,Wang SY,Lee KY,Li SL,Chen YP,Jinn TR,Tzen JT

更新日期：2011-07-01 00:00:00

abstract：AIM:To study the stereoselectivity in pharmacokinetics of the enantiomers of trans-tramadol (trans-T) and its active metabolite, trans-O-demethyltramadol (M1) in human subjects. METHODS:Trans-T hydrochloride sustained-release tablets were taken orally by 12 healthy male volunteers. After a multiple dosage schedule, th...

journal_title：Acta pharmacologica Sinica

authors： Liu HC,Liu TJ,Yang YY,Hou YN

更新日期：2001-01-01 00:00:00

abstract：AIM:Probucol, an anti-hyperlipidemic drug, has been reported to exert antitumor activities at various stages of tumor initiation, promotion and progression. In this study we examined whether the drug affected glioma cell growth in vitro and the underlying mechanisms. METHODS:Human glioma U87 and glioblastoma SF295 cel...

journal_title：Acta pharmacologica Sinica

authors： Jiang YS,Lei JA,Feng F,Liang QM,Wang FR

更新日期：2014-12-01 00:00:00

abstract：AIM:To examine the role of cholinergic neurons in the nucleus raphe magnus (NRM) in noxious heat stimulation and in the effects of morphine-induced antinociception by rats. METHODS:After the cholinergic neuron selective toxin, AF64A, was microinjected into the NRM, we examined changes in the antinociceptive threshold ...

journal_title：Acta pharmacologica Sinica

authors： Abe K,Ishida K,Kato M,Shigenaga T,Taguchi K,Miyatake T

更新日期：2002-11-01 00:00:00

abstract：:Cysteine-rich peptides from the venom of cone snails (Conus) target a wide variety of different ion channels. One family of conopeptides, the alpha-conotoxins, specifically target different isoforms of nicotinic acetylcholine receptors (nAChRs) found both in the neuromuscular junction and central nervous system. This ...

journal_title：Acta pharmacologica Sinica

authors： Azam L,McIntosh JM

更新日期：2009-06-01 00:00:00

abstract：:Pre-B-cell leukemia transcription factor 3 (PBX3) is a member of the PBX family and contains a highly conserved homologous domain. PBX3 is involved in the progression of gastric cancer, colorectal cancer, and prostate cancer; however, the detailed mechanism by which it promotes tumor growth remains to be elucidated. H...

journal_title：Acta pharmacologica Sinica

authors： Li WF,Herkilini A,Tang Y,Huang P,Song GB,Miyagishi M,Kasim V,Wu SR

更新日期：2021-02-01 00:00:00

abstract：:Astragaloside IV (AsIV) is an active saponin extracted from Astragalus membranaceus, which has shown cardioprotective effects in a number of experimental animals. In this study we investigated the molecular mechanisms by which AsIV attenuated the myocardial ischemia reperfusion (MI/R)-induced injury in vitro and in vi...

journal_title：Acta pharmacologica Sinica

authors： Yin B,Hou XW,Lu ML

更新日期：2019-05-01 00:00:00

abstract：AIM:Based on the structural analysis to reveal the mechanism of ligand binding to beta-secretase and the specificity of each binding sub-site. METHODS:Molecular dynamics was used to simulate on the ligand free beta-secretase and ligand bound beta-secretase. The trajectories were analyzed using the essential dynamics, ...

journal_title：Acta pharmacologica Sinica

authors： Xiong B,Huang XQ,Shen LL,Shen JH,Luo XM,Shen X,Jiang HL,Chen KX

更新日期：2004-06-01 00:00:00

abstract：AIM:To investigate the expression of advanced glycation end products (AGEs) and their receptor RAGE in the livers and blood vessels of rats with non-alcoholic steatohepatitis (NASH) and the effect of pentoxifylline (PTX) on liver and artery function in rats with NASH. METHODS:Sprague-Dawley rats were fed a high-fat di...

journal_title：Acta pharmacologica Sinica

authors： Wu J,Zhao MY,Zheng H,Zhang H,Jiang Y

更新日期：2010-10-01 00:00:00

abstract：:KCNQ1 channel is a member of the voltage-gated potassium channel KQT-like subfamily. The KCNQ1 gene has recently been identified as a susceptibility locus for type 2 diabetes mellitus (T2DM). In the present study, we examined the effects of KCNQ1 variants on the therapeutic response to modified-release gliclazide (gli...

journal_title：Acta pharmacologica Sinica

authors： Li Q,Tang TT,Jiang F,Zhang R,Chen M,Yin J,Bao YQ,Cheng X,Hu C,Jia WP

更新日期：2017-01-01 00:00:00

abstract：AIM:To investigate the effects and underlying mechanisms of 118, a novel derivative of mycophenolic acid, in a murine allogeneic skin graft model. METHODS:Skin grafts were conducted by grafting BALB/c donor tail skin into C57BL/6 skin beds (allograft) or by grafting female C57BL/6 donor tail skin into female C57BL/6 s...

journal_title：Acta pharmacologica Sinica

authors： Kong FY,Chen W,He SJ,Lin ZM,Li X,Zhang XH,Yang XQ,Zhu FH,Tong XK,Zhou Y,Tang W,Duan WH,Zuo JP

更新日期：2013-07-01 00:00:00

abstract：AIM:Omi is an ATP-independent serine protease that is necessary for neuronal function and survival. The aim of this study was to investigate the role of protease Omi in regulating differentiation of mouse neuroblastoma cells and to identify the substrate of Omi involved in this process. METHODS:Mouse neuroblastoma N2a...

journal_title：Acta pharmacologica Sinica

authors： Ma Q,Hu QS,Xu RJ,Zhen XC,Wang GH

更新日期：2015-08-01 00:00:00

abstract：AIM:To examine the therapeutic effects and underlying mechanisms of DZ2002, a reversible S-adenosyl-L-homocysteine hydrolase (SAHH) inhibitor, on lupus-prone female NZB×NZW F1 (NZB/W F1) mice. METHODS:Female NZB/W F1 mice were treated orally with DZ2002 (0.5 mg·kg(-1)·d(-1)) for 11 weeks, and the proteinuria level and...

journal_title：Acta pharmacologica Sinica

authors： He SJ,Lin ZM,Wu YW,Bai BX,Yang XQ,He PL,Zhu FH,Tang W,Zuo JP

更新日期：2014-02-01 00:00:00

abstract：AIM:The aim of the present study was to explore the mechanism for the Ca2+- dependent inactivation of the canonical transient receptor potential (TRPC) 7 channel expressed in human embryonic kidney 293 cells. METHOD:The whole-cell patch-clamp technique was used in the study. RESULTS:With Ca2+-free external solution, ...

journal_title：Acta pharmacologica Sinica

authors： Zhang H,Inoue R,Shi J,Jin XH,Li YQ

更新日期：2008-01-01 00:00:00

abstract：:Liver fibrosis is excessive accumulation of extracellular matrix proteins that results from various chronic liver diseases. Hepatic stellate cells (HSCs) play an essential role in the pathogenesis of liver fibrosis. Danggui Buxue Tang (DBT) is a classic formula of Chinese traditional medicine. We previously showed tha...

journal_title：Acta pharmacologica Sinica

authors： Guo T,Liu ZL,Zhao Q,Zhao ZM,Liu CH

更新日期：2018-09-01 00:00:00

abstract：AIM:To investigate the mechanisms underlying the isomer-selective distribution of 3-n-butylphthalide (NBP) hydroxylated metabolites, 3-hydroxy-NBP (3-OH-NBP) and 10-hydroxy-NBP (10-OH-NBP), across the blood brain barrier (BBB). METHODS:After oral administration of NBP (20 mg/kg) to rats, the pharmacokinetics of two ma...

journal_title：Acta pharmacologica Sinica

authors： Diao XX,Zhong K,Li XL,Zhong DF,Chen XY

更新日期：2015-12-01 00:00:00

abstract：AIM:Matrine is an alkaloid from Sophora alopecuroides L, which has shown a variety of pharmacological activities and potential therapeutic value in cardiovascular diseases. In this study we examined the protective effects of matrine against diabetic cardiomyopathy (DCM) in rats. METHODS:Male SD rats were injected with...

journal_title：Acta pharmacologica Sinica

authors： Liu ZW,Wang JK,Qiu C,Guan GC,Liu XH,Li SJ,Deng ZR

更新日期：2015-03-01 00:00:00

abstract：:Monoclonal antibodies are believed to be magic bullets and hold great potential for lots of biological process. About 100 μg of mAb109 was expressed in 5 × 106 cells after 10 days' immunization. 64Cu-NOTA-mAb109 was synthesized with the specific activity of 0.74 MBq/μg and high in vitro stability. The binding affinity...

journal_title：Acta pharmacologica Sinica

authors： Zhu H,Liu TL,Liu CH,Wang J,Zhang H,Dong B,Shen J,Zhao CK,Li ZF,Cheng Z,Yang Z

更新日期：2020-01-01 00:00:00

abstract：AIM:To examine the stimulatory effect of bacille Calmette-Gu rin (BCG) cell wall components on human beta-defensin-1 (hBD-1) gene expression and analyze the response element in the 5'-flanking region of the gene. METHODS:BCG cell wall proteins were fractionated by Sephadex G-150 chromatography. Using reverse-transcrip...

journal_title：Acta pharmacologica Sinica

authors： Zhu BD,Feng Y,Huang N,Wu Q,Wang BY

更新日期：2003-09-01 00:00:00

abstract：AIM:To study the effect of new antihepatitis drug, bicyclol, on the metabolism and hepatotoxicity of aflatoxin B1 (AFB1) in rats. METHODS:Rats were given bicyclol 300 mg/kg/d ig for 3 d and then injected ip with AFB1 1.5 mg/kg. Liver damages were examined 16 h after ip AFB1. The in vitro metabolism of AFB1 by bicyclol...

journal_title：Acta pharmacologica Sinica

authors： Lu H,Li Y

更新日期：2002-10-01 00:00:00