Isolation of acetylcholinesterase G4 and G1 molecular isoforms from rat cortex.

abstract：AIM:To investigate the proliferation of vascular smooth muscle cells (VSMC) affected by ginsenoside Rg1 and further explore the molecular mechanism of ginsenoside Rg1 using proteomics. METHODS:The proliferation of VSMC was measured by MTS assay kit and flow cytometry. Proteomic alterations were analyzed using two-dime...

journal_title：Acta pharmacologica Sinica

authors： Ma ZC,Gao Y,Wang YG,Tan HL,Xiao CR,Wang SQ

更新日期：2006-08-01 00:00:00

abstract：AIM:To examine the protective effect of propofol in renal ischemia/reperfusion (I/R) injury and the role of heme oxygenase-1 (HO-1) in this process. METHODS:Sprague-Dawley rats were randomly divided into 3 groups: (i) sham-operated group; (ii) I/R group; and (iii) propofol group. Bilateral renal warm ischemia for 45 m...

journal_title：Acta pharmacologica Sinica

authors： Wang HH,Zhou HY,Chen CC,Zhang XL,Cheng G

更新日期：2007-08-01 00:00:00

abstract：AIM:To discover novel cell division cycle 25 (CDC25) B inhibitors and elucidate the mechanisms of inhibition in cancer cells. METHODS:Cell growth inhibition was detected by MTT assay, the cell cycle was analyzed by flow cytometry, and protein expression and phosphorylation was examined by Western blot analysis. RESUL...

journal_title：Acta pharmacologica Sinica

authors： Zhou YB,Feng X,Wang LN,Du JQ,Zhou YY,Yu HP,Zang Y,Li JY,Li J

更新日期：2009-09-01 00:00:00

abstract：AIM:To model the three-dimensional structure and investigate the interaction mechanism of the proprotein convertase furin/kexin and their inhibitors (eglin c mutants). METHODS:The three-dimensional complex structures of furin/kexin with its inhibitors, eglin c mutants, were generated by modeller program using the newl...

journal_title：Acta pharmacologica Sinica

authors： Cai XH,Zhang Q,Ding DF

更新日期：2004-12-01 00:00:00

abstract：AIM:G protein-coupled inwardly rectifying potassium channels (GIRK) are important for neuronal signaling and membrane excitability. In the present study, we intend to find whether GIRK channels express functionally in adult rat dorsal root ganglion (DRG) neurons. METHODS:We used RT-PCR to detect mRNA for 4 subunits of...

journal_title：Acta pharmacologica Sinica

authors： Gao XF,Zhang HL,You ZD,Lu CL,He C

更新日期：2007-02-01 00:00:00

abstract：AIM:Toxicity of retinol (vitamin A) has been previously associated with apoptosis and/or cell malignant transformation. Thus, we investigated the pathways involved in the induction of proliferation, deformation and proliferative focus formation by retinol in cultured Sertoli cells of rats. METHODS:Sertoli cells were i...

journal_title：Acta pharmacologica Sinica

authors： Gelain DP,Pasquali MA,Caregnato FF,Castro MA,Moreira JC

更新日期：2012-04-01 00:00:00

abstract：AIM:To evaluate the protective effects of apocynin on "two-hit" injury in rats. METHODS:"Two-hit" injury model of rat was induced by hemorrhagic shock (40 mmHg for 45 min) followed by iv administration of lipopolysaccharide (LPS, 150 microg/kg). Rats were randomized into seven groups: Sham, LPS, hemorrhage, hemorrhage...

journal_title：Acta pharmacologica Sinica

authors： Zhou R,Hu DY,Liu LM,Zhou XW

更新日期：2002-11-01 00:00:00

abstract：AIM:To evaluate the effect of inhaled formoterol-budesonide on airway remodeling in adult patients with moderate asthma. METHODS:Thirty asthmatic patients and thirty control subjects were enrolled. Asthmatic subjects used inhaled Symbicort 4.5/160 μg twice daily for one year. The effect of formoterol-budesonide on air...

journal_title：Acta pharmacologica Sinica

authors： Wang K,Liu CT,Wu YH,Feng YL,Bai HL,Ma ES,Wen FQ

更新日期：2011-01-01 00:00:00

abstract：:Binding of a neurotransmitter to its ionotropic receptor opens a distantly located ion channel, a process termed allosteric activation. Here we review recent advances in the molecular mechanism by which the cys-loop receptors are activated with emphasis on the best studied nicotinic acetylcholine receptors (nAChRs). W...

journal_title：Acta pharmacologica Sinica

authors： Chang YC,Wu W,Zhang JL,Huang Y

更新日期：2009-06-01 00:00:00

abstract：AIM:To investigate the metabolic characteristics of cytochrome P-450 C YP2A6 in human liver microsomes. METHODS:Cytochrome P-450 enzyme activities were measured by biochemical assays. Xenobiotics were employed to observe their effects on CYP2A6 in vitro. The kinetics of coumarin 7-hydroxylase was determined, and the c...

journal_title：Acta pharmacologica Sinica

authors： Xia XY,Peng RX,Yu JP,Wang H,Wang J

更新日期：2002-05-01 00:00:00

abstract：:Kushen (Radix Sophorae Flavescentis) has a long history of use for the treatment of tumors, inflammation and other diseases in traditional Chinese medicine. Compound Kushen Injection (CKI) is a mixture of natural compounds extracted from Kushen and Baituling (Rhizoma Smilacis Glabrae). The main principles of CKI are m...

journal_title：Acta pharmacologica Sinica

authors： Wang W,You RL,Qin WJ,Hai LN,Fang MJ,Huang GH,Kang RX,Li MH,Qiao YF,Li JW,Li AP

更新日期：2015-06-01 00:00:00

abstract：AIM:To investigate whether sodium ferulate (SF) can protect cortical neurons from glutamate-induced neurotoxicity and the mechanisms responsible for this protection. METHODS:Cultured cortical neurons were incubated with 50 micromol/L glutamate for either 30 min or 24 h, with or without pre-incubation with SF (100, 200...

journal_title：Acta pharmacologica Sinica

authors： Jin Y,Yan EZ,Fan Y,Guo XL,Zhao YJ,Zong ZH,Liu Z

更新日期：2007-12-01 00:00:00

abstract：AIM:To investigate whether IL-12p40 plays a crucial role in regulating islet allograft rejection in a streptozotocin (STZ)-induced diabetes mouse model. METHODS:C57BL/6 and IL-12p40 gene knockout mice were selected as recipient mice, to which the diabetes was induced with a treatment of STZ (150-200 mg/kg) by a single...

journal_title：Acta pharmacologica Sinica

authors： Bi EG,Shi W,Zou J,Hao ZH,Li ZH,Cai D,Zhang HQ,Sun B

更新日期：2006-08-01 00:00:00

abstract：:Embryonic stem (ES) cells and their differentiated progeny offer tremendous potential for regenerative medicine, even in the field of drug discovery. There is an urgent need for clinically relevant assays that make use of ES cells because of their rich biological utility. Attention has been focused on small molecules ...

journal_title：Acta pharmacologica Sinica

authors： Lou YJ,Liang XG

更新日期：2011-02-01 00:00:00

abstract：AIM:A discrimination analysis has been explored for the probabilistic classification of healthy versus ovarian cancer serum samples using proteomics data from mass spectrometry (MS). METHODS:The method employs data normalization, clustering, and a linear discriminant analysis on surface-enhanced laser desorption ioniz...

journal_title：Acta pharmacologica Sinica

authors： Hong YJ,Wang XD,Shen D,Zeng S

更新日期：2008-10-01 00:00:00

abstract：AIM:Breviscapine isolated from the Chinese herb Erigeron breviscapus (Vant) Hand-Mazz is widely used to treat cardiovascular and cerebrovascular diseases. The aim of this study was to improve the pharmacokinetic profiles of breviscapine using nanostructured lipid carrier based on an ionic complex formation. METHODS:Br...

journal_title：Acta pharmacologica Sinica

authors： Li M,Zheng Y,Shan FY,Zhou J,Gong T,Zhang ZR

更新日期：2013-08-01 00:00:00

abstract：:In an effort to develop effective alternative strategies that increase the therapeutic efficacy and minimize the systemic toxicity of chemotherapeutic agents, more efforts are being directed towards the investigation of dietary supplements and other phytotherapeutic agents for their synergistic efficacy in combination...

journal_title：Acta pharmacologica Sinica

authors： Raina K,Agarwal R

更新日期：2007-09-01 00:00:00

abstract：:Recent evidence shows that high glucose levels recruit carbohydrate response element-binding protein, which binds the promoter of thioredoxin-interacting protein (txnip), thereby regulating its expression in β-cells. Overexpression of txnip not only induces β-cell apoptosis but also reduces insulin production. Thus, t...

journal_title：Acta pharmacologica Sinica

authors： Li T,Lin GY,Zhong L,Zhou Y,Wang J,Zhu Y,Feng Y,Cai XQ,Liu Q,Nosjean O,Boutin JA,Renard P,Yang DH,Wang MW

更新日期：2017-07-01 00:00:00

abstract：AIM:To study the effects of tetrandrine (Tet) on extracellular Ca2+ influx and intracellular Ca2+ release in cultured calf basilar artery smooth muscle cells. METHODS:Free intracellular calcium was examined by a system of measurement of AR-CM-MIC, using Fura 2-AM as a fluorescent indicator. RESULTS:In the presence of...

journal_title：Acta pharmacologica Sinica

authors： Wang B,Xiao JG

更新日期：2002-12-01 00:00:00

abstract：AIM:To study the protective effect of the polypeptide isolated from Chlamys farreri (PCF) on hairless mice skin damaged by ultraviolet A. METHODS:Enzymes and malondialdehyde (MDA) were determined by biochemical methods; the expressions of Bcl-2 protein and NOS protein were examined by immunohistochemical technique. Th...

journal_title：Acta pharmacologica Sinica

authors： Wang CB,Yao RY,Liu ZT,Zhong WZ,Liu XP,Wang YJ

更新日期：2002-09-01 00:00:00

abstract：AIM:To explore the anticancer effects and the molecular mechanisms of gambogic acid (GA) on Jurkat cells. METHODS:Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Annexin V-fluorescein-isothiocyanate/propidium iodide, DNA defragmentation, and comet assay were used to d...

journal_title：Acta pharmacologica Sinica

authors： Wang Y,Chen Y,Chen Z,Wu Q,Ke WJ,Wu QL

更新日期：2008-03-01 00:00:00

abstract：AIM:Based on the structural analysis to reveal the mechanism of ligand binding to beta-secretase and the specificity of each binding sub-site. METHODS:Molecular dynamics was used to simulate on the ligand free beta-secretase and ligand bound beta-secretase. The trajectories were analyzed using the essential dynamics, ...

journal_title：Acta pharmacologica Sinica

authors： Xiong B,Huang XQ,Shen LL,Shen JH,Luo XM,Shen X,Jiang HL,Chen KX

更新日期：2004-06-01 00:00:00

abstract：:For follicular lymphoma (FL) with grade 1/2, the complete response (CR) rate of the first-line R-CHOP treatment was significantly low. In this study, we assessed the rationality of the administration of rituximab for FL patients with grade 1/2 based on concentration-response relationship analyses. Thus, we conducted a...

journal_title：Acta pharmacologica Sinica

authors： Liu S,Huang H,Chen RX,Wang Z,Guan YP,Peng C,Fang XJ,Chen ZJ,Guan SX,Zhu X,Ren QG,Yao YY,Huang HB,Huang M,Wang XD,Lin TY

更新日期：2020-07-31 00:00:00

abstract：AIM:To examine the role of atorvastatin on volume-overload-induced heart failure and to test the hypothesis that atorvastatin inhibits MMP-2 and 9 expression in heart failure with non-ischemic etiology. METHODS:Arteriovenous (AV) fistula-treated rats were administered with atorvastatin (3 mg/kg/d) or vehicle for 17 we...

journal_title：Acta pharmacologica Sinica

authors： Cheng G,Xu G,Cai HW,Wang HH,Bao XF

更新日期：2007-04-01 00:00:00

abstract：:Oral administration of curcumin has been shown to inhibit pulmonary fibrosis (PF) despite its extremely low bioavailability. In this study, we investigated the mechanisms underlying the anti-PF effect of curcumin in focus on intestinal endocrine. In bleomycin- and SiO2-treated mice, curcumin (75, 150 mg· kg-1 per day)...

journal_title：Acta pharmacologica Sinica

authors： Miao YM,Zhang YJ,Qiao SM,Xia YF,Wei ZF,Dai Y

更新日期：2020-07-21 00:00:00

abstract：AIM:The role of caveolin-1 (Cav-1) in angiogenesis remains poorly understood. The endothelial nitric oxide (NO) synthase (eNOS), a caveolin-interacting protein, was demonstrated to play a predominant role in vascular endothelial growth factor (VEGF) -induced angiogenesis. The purpose of our study was to examine the rol...

journal_title：Acta pharmacologica Sinica

authors： Pan YM,Yao YZ,Zhu ZH,Sun XT,Qiu YD,Ding YT

更新日期：2006-12-01 00:00:00

abstract：AIM:To investigate the effect of epigallocatechin gallate (EGCG) on angiotensin II (Ang II)-induced stress fiber formation and hyperpermeability in endothelial cells. METHODS:Human umbilical vein endothelial cells (HUVECs) were treated with Ang II in the absence or presence of EGCG or mitogen-activated protein kinases...

journal_title：Acta pharmacologica Sinica

authors： Yang D,Liu J,Tian C,Zeng Y,Zheng YH,Fang Q,Li HH

更新日期：2010-10-01 00:00:00

abstract：:In drug design and discovery, binding affinity and selectivity are two basic properties of a drug candidate. Opioid receptors (ORs) are the main targets of strong analgesics. Like some other class A members of G-protein-coupled receptors (GPCRs), ORs exhibit complex selectivity on their ligands. The diversity of bindi...

journal_title：Acta pharmacologica Sinica

authors： Cheng JX,Cheng T,Li WH,Liu GX,Zhu WL,Tang Y

更新日期：2018-03-01 00:00:00

abstract：:Recent evidence shows that raddeanin A (RA), an oleanane-type triterpenoid saponin extracted from Anemone raddeana Regel, exerts remarkable cytotoxicity against cancer cells in vitro and in vivo. In addition, RA has also been found to activate autophagy in human gastric cancer cells. In this study, we investigated the...

journal_title：Acta pharmacologica Sinica

authors： Guan YD,Jiang SL,Yu P,Wen M,Zhang Y,Xiao SS,Xu XJ,Cheng Y

更新日期：2018-04-01 00:00:00

abstract：:Resibufogenin (RBG) is a single compound isolated from Chansu, a traditional Chinese medicine obtained from the skin venom gland of the toad. Formulations of Chansu have been widely applied in China, Japan, and other Asian countries for a long time and are currently used as alternative medicines. However, there have b...

journal_title：Acta pharmacologica Sinica

authors： Xie JT,Dey L,Wu JA,Lowell TK,Yuan CS

更新日期：2001-04-01 00:00:00