Discrimination analysis of mass spectrometry proteomics for ovarian cancer detection.

abstract：:Parkinson's disease (PD) is a common neurodegenerative disease characterized by motor impairment and progressive loss of dopamine (DA) neurons. At present, the acute application of neurotoxic drugs such as 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 6-hydroxydopamine (6-OHDA) are commonly used to simulate ...

journal_title：Acta pharmacologica Sinica

authors： Guo CH,Cao T,Zheng LT,Waddington JL,Zhen XC

更新日期：2020-04-01 00:00:00

abstract：AIM:To examine the in vitro antitumor activity of resveratrol against multiple myeloma (MM) cell lines (RPMI 8226, U266, and KM3), and the mechanisms involved. METHODS:The growth inhibition of resveratrol was determined by 3-(4, 5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The effect of resve...

journal_title：Acta pharmacologica Sinica

authors： Sun CY,Hu Y,Guo T,Wang HF,Zhang XP,He WJ,Tan H

更新日期：2006-11-01 00:00:00

abstract：AIM:To make a LQT3 model (one form of the long QT syndromes) and to investigate the effect of mexiletine on LQT3. METHODS:Sea anemone toxin (ATX II) was used to produce the LQT3 model. The Effect of mexiletine on LQT3 was performed on single Na channel, action potential, and electrocardiography in guinea pigs. RESULT...

journal_title：Acta pharmacologica Sinica

authors： Wang HW,Zheng YQ,Yang ZF,Li CZ,Liu YM

更新日期：2003-04-01 00:00:00

abstract：AIM:To evaluate the effect of C-C chemokine receptor 3 (CCR3) blockade on pulmonary inflammation and mucus production in allergic mice. METHODS:We used the synthetic peptide of the CCR3 NH2-terminal as the immunizing antigen and generated murine monoclonal antibody against the human CCR3. In addition, the generated an...

journal_title：Acta pharmacologica Sinica

authors： Wang K,Shen HH,Li W,Huang HQ

更新日期：2007-11-01 00:00:00

abstract：AIM:To search for novel effective P-glycoprotein (P-gp) reversal agents in the blood-brain barrier (BBB). METHODS:Using rhodamine123 (Rh123) to examine the functional activity of P-gp in cultured bovine brain capillary endothelial cells (BCEC) and screen various principles on P-gp modulation in BBB. RESULTS:All of te...

journal_title：Acta pharmacologica Sinica

authors： He L,Liu GQ

更新日期：2002-07-01 00:00:00

abstract：AIM:To investigate whether myosin light chain kinase (MLCK) contributed to the high proliferative ability of breast cancer cells. METHODS:Soft agar colony formation on the MCF-7 and LM-MCF-7 cell lines was determined. The cell cycles of MCF-7 and LM-MCF-7 were detected using flow cytometry analysis. Western blot analy...

journal_title：Acta pharmacologica Sinica

authors： Cui WJ,Liu Y,Zhou XL,Wang FZ,Zhang XD,Ye LH

更新日期：2010-06-01 00:00:00

abstract：:The signal transducer and activator of transcription 3 (STAT3) plays a critical role in platelet functions. This study sought to understand the effects of the STAT3 inhibitor SC99 on platelet activation and aggregation. Immunoblotting assays were applied to measure the effects of SC99 on the STAT3 signaling pathway. A...

journal_title：Acta pharmacologica Sinica

authors： Xu Z,Xu YJ,Hao YN,Ren LJ,Zhang ZB,Xu X,Cao BY,Dai KS,Zhu L,Fang Q,Kong Y,Mao XL

更新日期：2017-05-01 00:00:00

abstract：AIM:Our previous study indicated that there are two types of Ca2+ release events seen in intact mouse bladder tissue. In this study our aim is to investigate the mechanism that underlies the phenomena of Ca2+ release in smooth muscle. METHODS:Single cells were isolated and tissue segments were prepared by cutting the ...

journal_title：Acta pharmacologica Sinica

authors： Wang M,Chen Z,Xing Y,Zhang X,Dong XZ,Ji GJ

更新日期：2006-07-01 00:00:00

abstract：AIM:To investigate the role of c-Jun N-terminal protein kinase 1 and 2 (JNK1/2) and the main signal pathway for its activation in hydrogen peroxide (H(2)O(2)) induced apoptotic-like cortical cell death. METHODS:Using the model of oxidative stress induced by H(2)O(2), the expression and diphosphorylation of JNK1/2 was ...

journal_title：Acta pharmacologica Sinica

authors： Wang W,Gao C,Hou XY,Liu Y,Zong YY,Zhang GY

更新日期：2004-05-01 00:00:00

abstract：AIM:To evaluate the nasal absorption enhancement of insulin by sodium deoxycholate (SDC) in combination with cyclodextrins (CD). METHODS:The concentration of glucose in blood was measured. Scanning electron microscopy technique was used to investigate the effect of enhancers on the nasal mucocilia. The effect of SDC i...

journal_title：Acta pharmacologica Sinica

authors： Zhang Y,Jiang XG,Yao J

更新日期：2001-11-01 00:00:00

abstract：AIM:To develop and validate a novel precolumn derivatization method for the quantitative determination and pharmacokinetic application of acetylshikonin in macaque monkeys by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). METHODS:2-Mercaptoethanol was added to the blood sample a...

journal_title：Acta pharmacologica Sinica

authors： Sun DX,Tian HF,Meng ZY,Du A,Yuan D,Gu RL,Wu ZN,Dou GF

更新日期：2008-12-01 00:00:00

abstract：AIM:Experimental studies found that cinacalcet (CINA) markedly attenuated vascular calcification in uremic rats, but its underlying mechanisms are still largely unknown. Recent evidence have demonstrated that endothelial cells (ECs) participate in ectopic calcification in part by mediating endothelial-to-mesenchymal tr...

journal_title：Acta pharmacologica Sinica

authors： Wu M,Tang RN,Liu H,Pan MM,Liu BC

更新日期：2016-11-01 00:00:00

abstract：AIM:To investigate the effect of 7-hydroxystaurosporine (UCN-01), a selective protein kinase C (PKC) inhibitor, on cell growth, migration, and invasion in invasive human glioblastoma U-87MG cells. METHODS:PKC activity was determined based on the PKC-catalyzed transfer of the (32)P-phosphate group from [g-(32)P]ATP int...

journal_title：Acta pharmacologica Sinica

authors： Meng QH,Zhou LX,Luo JL,Cao JP,Tong J,Fan SJ

更新日期：2005-04-01 00:00:00

abstract：AIM:To investigate the effects of the cardiotonic steroid, ouabain, on cardiac differentiation of murine embyronic stem cells (mESCs). METHODS:Cardiac differentiation of murine ESCs was enhanced by standard hanging drop method in the presence of ouabain (20 μmol/L) for 7 d. The dissociated ES derived cardiomyocytes we...

journal_title：Acta pharmacologica Sinica

authors： Lee YK,Ng KM,Lai WH,Man C,Lieu DK,Lau CP,Tse HF,Siu CW

更新日期：2011-01-01 00:00:00

abstract：AIM:To study whether urotensin II (UII), a potent vasoconstrictive peptide, is involved in the development of cardiac hypertrophy and fibrogenesis of rats induced by isoproterenol (ISO). METHODS:Thirty male Wistar rats were randomly divided into 3 groups. Group 1 was the healthy control group, group 2 was the ISO grou...

journal_title：Acta pharmacologica Sinica

authors： Zhang YG,Li YG,Liu BG,Wei RH,Wang DM,Tan XR,Bu DF,Pang YZ,Tang CS

更新日期：2007-01-01 00:00:00

abstract：AIM:To develop a high-throughput real-time assay based on molecular beacons to monitor the integrase 3'-processing reaction in vitro and apply it to inhibitor screening. METHODS:The recombinant human immunodeficiency virus (HIV)-1 integrase (IN) is incubated with a 38 mer oligonucleotide substrate, a sequence identica...

journal_title：Acta pharmacologica Sinica

authors： He HQ,Ma XH,Liu B,Zhang XY,Chen WZ,Wang CX,Cheng SH

更新日期：2007-06-01 00:00:00

abstract：AIM:Endothelium-dependent relaxations to certain neurohumoral substances are mediated by pertussis toxin-sensitive Gi/o protein. Our experiments were designed to determine the role, if any, of pertussis toxin-sensitive G-proteins in relaxations attributed to endothelium-derived hyperpolarizing factor (EDHF). METHODS:P...

journal_title：Acta pharmacologica Sinica

authors： Ng KF,Leung SW,Man RY,Vanhoutte PM

更新日期：2008-12-01 00:00:00

abstract：:Saw palmetto extract (SPE), an extract from the ripe berries of the American dwarf palm, has been widely used as a therapeutic remedy for urinary dysfunction due to benign prostatic hyperplasia (BPH) in Europe. Numerous mechanisms of action have been proposed for SPE, including the inhibition of 5alpha-reductase. Toda...

journal_title：Acta pharmacologica Sinica

authors： Suzuki M,Ito Y,Fujino T,Abe M,Umegaki K,Onoue S,Noguchi H,Yamada S

更新日期：2009-03-01 00:00:00

abstract：AIM:To investigate the influence of picroside II on hepatocyte apoptosis and its mechanism. METHODS:Morphological changes and quantification of apoptotic cells were determined under transmission electron microscopy and flow cytometry respectively. DNA fragmentation was visualized by agarose gel electrophoresis. Semi-q...

journal_title：Acta pharmacologica Sinica

authors： Gao H,Zhou YW

更新日期：2005-06-01 00:00:00

abstract：:Oral administration of curcumin has been shown to inhibit pulmonary fibrosis (PF) despite its extremely low bioavailability. In this study, we investigated the mechanisms underlying the anti-PF effect of curcumin in focus on intestinal endocrine. In bleomycin- and SiO2-treated mice, curcumin (75, 150 mg· kg-1 per day)...

journal_title：Acta pharmacologica Sinica

authors： Miao YM,Zhang YJ,Qiao SM,Xia YF,Wei ZF,Dai Y

更新日期：2020-07-21 00:00:00

abstract：AIM:To investigate the influences of betulinic acid (BA), a triterpenoid isolated from birch bark, on neuroinflammatory mediators involved in the pathogenesis of multiple sclerosis and experimental autoimmune encephalomyelitis in vitro. METHODS:Encephalitogenic T cells were prepared from draining lymph nodes and spina...

journal_title：Acta pharmacologica Sinica

authors： Blaževski J,Petković F,Momčilović M,Paschke R,Kaluđerović GN,Mostarica Stojković M,Miljković D

更新日期：2013-03-01 00:00:00

abstract：:Breast cancer resistance protein (BCRP) is one of ATP-binding cassette (ABC) transporters in brain microvessel endothelial cells that transport their substrates from brain to blood, thus limiting substrates to crossing into brain through blood-brain barrier. Our previous works show that bile duct ligation (BDL) impair...

journal_title：Acta pharmacologica Sinica

authors： Qin YY,Xu P,Wu T,Qian CQ,Fan YL,Gen DH,Zhu L,Kong WM,Yang HY,Xu F,Yang YT,Liu L,Liu XD

更新日期：2020-02-01 00:00:00

abstract：AIM:To investigate the antidepressant-like effects of a novel 5-HT3 receptor antagonist N-(benzo[d]thiazol-2-yl)-3-methoxyquinoxalin-2-carboxamide (6z) in acute and chronic murine models of depression. METHODS:5-HT3 receptor antagonism was examined in guinea pig ileum in vitro. A tail suspension test (TST) was used as...

journal_title：Acta pharmacologica Sinica

authors： Gupta D,Radhakrishnan M,Kurhe Y,Thangaraj D,Prabhakar V,Kanade P

更新日期：2014-12-01 00:00:00

abstract：AIM:To study the effects of 3-n-butylphthalide (NBP) on the TREK-1 channel expressed in Chinese hamster ovary (CHO) cells. METHODS:Whole-cell patch-clamp recording was used to record TREK-1 channel currents. The effects of varying doses of l-NBP on TREK-1 currents were also observed. Current-clamp recordings were perf...

journal_title：Acta pharmacologica Sinica

authors： Ji XC,Zhao WH,Cao DX,Shi QQ,Wang XL

更新日期：2011-02-01 00:00:00

abstract：:Binding of a neurotransmitter to its ionotropic receptor opens a distantly located ion channel, a process termed allosteric activation. Here we review recent advances in the molecular mechanism by which the cys-loop receptors are activated with emphasis on the best studied nicotinic acetylcholine receptors (nAChRs). W...

journal_title：Acta pharmacologica Sinica

authors： Chang YC,Wu W,Zhang JL,Huang Y

更新日期：2009-06-01 00:00:00

abstract：:Resibufogenin (RBG) is a single compound isolated from Chansu, a traditional Chinese medicine obtained from the skin venom gland of the toad. Formulations of Chansu have been widely applied in China, Japan, and other Asian countries for a long time and are currently used as alternative medicines. However, there have b...

journal_title：Acta pharmacologica Sinica

authors： Xie JT,Dey L,Wu JA,Lowell TK,Yuan CS

更新日期：2001-04-01 00:00:00

abstract：AIM:To investigate the molecular mechanism and signaling pathway by which fenoterol, a beta(2)-adrenergic receptor (beta(2)-AR) agonist, produces anti-inflammatory effects. METHODS:THP-1, a monocytic cell line, was used to explore the mechanism of beta(2)-AR stimulation in LPS-induced secretion of inflammatory cytokin...

journal_title：Acta pharmacologica Sinica

authors： Wang W,Xu M,Zhang YY,He B

更新日期：2009-11-01 00:00:00

abstract：AIM:To examine the effect of curcumin on oxidative DNA damage and cell apoptosis and injury caused by the reaction of methylglyoxal(MG) with amino acids. METHODS:We used DNA strand breaks to examine the effect of curcumin on oxidative DNA damage. In addition, reactive oxygen species(ROS) formation occurs in MG-treated...

journal_title：Acta pharmacologica Sinica

authors： Chan WH,Wu HJ

更新日期：2006-09-01 00:00:00

abstract：:As the most powerful antigen-presenting cell type, dendritic cells (DCs) can induce potent antigen-specific immune responses in vivo, hence becoming optimal cell population for vaccination purposes. DCs can be derived ex vivo in quantity and manipulated extensively to be endowed with adequate immune-stimulating capaci...

journal_title：Acta pharmacologica Sinica

authors： Gu YZ,Zhao X,Song XR

更新日期：2020-07-01 00:00:00

abstract：AIM:To evaluate the pharmacokinetic characteristics of L-valyl-ara-C, a peptidomimetic prodrug of ara-C. METHODS:After the synthesis of L-valyl-ara-C, the in vitro stability of L-valyl-ara-C was examined in various biological media. Plasma pharmacokinetic profiles of ara-C and L-valyl-ara-C were also evaluated in rats...

journal_title：Acta pharmacologica Sinica

authors： Cheon EP,Han HK

更新日期：2007-02-01 00:00:00