Human C-C chemokine receptor 3 monoclonal antibody inhibits pulmonary inflammation in allergic mice.

abstract：AIM:To investigate the effect of aluminum (Al) on high voltage-dependent calcium current (I(HVA)) and its modulation by ginkgolide B (Gin B). METHODS:The whole-cell, patch-clamp technique was used to record IHVA from acutely isolated hippocampal CA1 pyramydal neurons in rats. RESULTS:Al 0.1 mmol/L (low concentration)...

journal_title：Acta pharmacologica Sinica

authors： Chen L,Liu CJ,Tang M,Li A,Hu XW,Du YM,Shen JJ,Lu YL,Heschler J

更新日期：2005-05-01 00:00:00

abstract：AIM:To investigate the mechanism of action of a potent analgesic, (+/-)-meptazinol. METHODS:The structures of meptazinol enantiomers were compared with opioid pharmacophore and tramadol. RESULTS:Neither enantiomer of meptazinol fitted any patterns among the opioid pharmacophore and tramadol, although they did share s...

journal_title：Acta pharmacologica Sinica

authors： Li W,Hao JL,Tang Y,Chen Y,Qiu ZB

更新日期：2005-03-01 00:00:00

abstract：AIM:Diabetic nephropathy is one of the major complications of diabetes and the major cause of end-stage renal disease. In this study we investigated the insulin deficiency (ID) induced changes in renal mesangial cells (MCs) and in the kidney of STZ-induced diabetic rats. METHODS:Cultured rat renal MCs were incubated i...

journal_title：Acta pharmacologica Sinica

authors： Kong YL,Shen Y,Ni J,Shao DC,Miao NJ,Xu JL,Zhou L,Xue H,Zhang W,Wang XX,Lu LM

更新日期：2016-02-01 00:00:00

abstract：AIM:To investigate the pharmacokinetic profile and tissue distribution of a novel phosphodiesterase type 5 inhibitor, 5-ethyl-2-{5-[4-(2-hydroxy-ethyl)-piperazine-1-sulfonyl]-2-propoxy-phenyl}-7-propyl-3,5-dihydro-pyrrolo(3,2-d)pyrimidin-4-one (SK-3530), in rats after administration of the (14)C-labeled compound. METH...

journal_title：Acta pharmacologica Sinica

authors： Yoo HH,Kim NS,Im GJ,Kim DH

更新日期：2007-08-01 00:00:00

abstract：:Central nervous system (CNS) disorders represent a broad spectrum of brain ailments with short- and long-term disabilities, and nanomedicine-based approaches provide a new therapeutic approach to treating CNS disorders. A variety of potential drugs have been discovered to treat several neuronal disorders; however, the...

journal_title：Acta pharmacologica Sinica

authors： Hanif S,Muhammad P,Chesworth R,Rehman FU,Qian RJ,Zheng M,Shi BY

更新日期：2020-07-01 00:00:00

abstract：:Parkinson's disease (PD) is a common neurodegenerative disease characterized by motor impairment and progressive loss of dopamine (DA) neurons. At present, the acute application of neurotoxic drugs such as 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 6-hydroxydopamine (6-OHDA) are commonly used to simulate ...

journal_title：Acta pharmacologica Sinica

authors： Guo CH,Cao T,Zheng LT,Waddington JL,Zhen XC

更新日期：2020-04-01 00:00:00

abstract：AIM:To discover novel ligands of estrogen receptor (ER) β using pharmacophore mapping and structure-based screening. METHODS:A computer-aided strategy combining pharmacophore mapping and structure-based screening was used to screen the Maybridge and Enamine databases. Yeast two-hybrid (Y2H) assay was used to detect th...

journal_title：Acta pharmacologica Sinica

authors： Chen L,Wu D,Bian HP,Kuang GL,Jiang J,Li WH,Liu GX,Zou SE,Huang J,Tang Y

更新日期：2014-10-01 00:00:00

abstract：AIM:To investigate the effect of tricyclodecane-9-yl-xanthogenate (D609) on cell differentiation in vascular endothelial cells (VECs) and marrow stromal cells (MSCs). METHODS:Morphological changes were observed under phase contrast microscope. Electron microscope and immunostaining were used for VECs identification. T...

journal_title：Acta pharmacologica Sinica

authors： Wang N,Du CQ,Wang SS,Xie K,Zhang SL,Miao JY

更新日期：2004-04-01 00:00:00

abstract：AIM:To examine the protective effect of propofol in renal ischemia/reperfusion (I/R) injury and the role of heme oxygenase-1 (HO-1) in this process. METHODS:Sprague-Dawley rats were randomly divided into 3 groups: (i) sham-operated group; (ii) I/R group; and (iii) propofol group. Bilateral renal warm ischemia for 45 m...

journal_title：Acta pharmacologica Sinica

authors： Wang HH,Zhou HY,Chen CC,Zhang XL,Cheng G

更新日期：2007-08-01 00:00:00

abstract：AIM:To express dopamine D1 receptor in baculovirus-Sf9 cell system, and to investigate the effects of l-12-chloroscoulerine (l-CSL) on the recombinant D1 receptor (D1R). METHODS:The recombinant baculovirus, Autographa californica nuclear polyhedrosis virus bearing D1R (AcNPV- D1R) was generated, and then was used to p...

journal_title：Acta pharmacologica Sinica

authors： He Y,Jin WQ,Shen QX,Chen XJ,Jin GZ

更新日期：2003-03-01 00:00:00

abstract：AIM:The feasibility of inducing endocrine pancreatic differentiation of embryonic stem (ES) cells has been well documented. However, whether ES cells possess the potential for exocrine pancreatic differentiation requires further exploration. Here, we investigated whether sodium butyrate and glucocorticoids were conduci...

journal_title：Acta pharmacologica Sinica

authors： Ren M,Yan L,Shang CZ,Cao J,Li FP,Li JY,Cheng H,Min J

更新日期：2009-09-01 00:00:00

abstract：AIM:To investigate the influence of trimetazidine, which is known to be an antioxidant and modulator of metabolism, on cardiac function and the development of diabetic cardiomyopathy in db/db mouse. METHODS:Trimetazidine was administered to db/db mice for eight weeks. Cardiac function was measured by inserting a Milla...

journal_title：Acta pharmacologica Sinica

authors： Li YJ,Wang PH,Chen C,Zou MH,Wang DW

更新日期：2010-05-01 00:00:00

abstract：AIM:To acquire the specific RNA aptamers inhibiting human red blood cell (RBC) acetylcholinesterase (AChE). METHODS:Systematic evolution of ligands by exponential enrichment (SELEX) aptamer against human red blood cell membrane AChE was selected by microtiter plate method in vitro. The specifity binding to AChE was de...

journal_title：Acta pharmacologica Sinica

authors： Zhang XM,Shao NS,Chi MG,Sun MJ

更新日期：2003-07-01 00:00:00

abstract：:Retinoid X receptor-α (RXRα), a unique member of the nuclear receptor superfamily, represents an intriguing and unusual target for pharmacologic interventions and therapeutic applications in cancer, metabolic disorders and neurodegenerative diseases. Despite the fact that the RXR-based drug Targretin (bexarotene) is c...

journal_title：Acta pharmacologica Sinica

authors： Zhang XK,Su Y,Chen L,Chen F,Liu J,Zhou H

更新日期：2015-01-01 00:00:00

abstract：AIM:To examine whether selective serotonin reuptake inhibitor citalopram interacts with Kv1.5, one of the cardiovascular-specific Kv channel isoforms. METHODS:The interaction between citalopram and Kv1.5 expressed in Chinese hamster ovary cells was studied using the whole-cell patch-clamp technique. RESULTS:Citalopra...

journal_title：Acta pharmacologica Sinica

authors： Lee HM,Hahn SJ,Choi BH

更新日期：2010-04-01 00:00:00

abstract：AIM:The non-neuronal acetylcholine system (NNAS) in endothelial cells participates in modulating endothelial function, vascular tone, angiogenesis and inflammation, thus plays a critical role in cardiovascular diseases. In this study, we used a proteomic approach to study potential downstream receptor-effectors of NNAS...

journal_title：Acta pharmacologica Sinica

authors： Zhang YY,Shen W,Zhang LC,Pan ZY,Long CL,Cui WY,Zhang YF,Wang H

更新日期：2014-09-01 00:00:00

abstract：AIM:To develop and validate a novel precolumn derivatization method for the quantitative determination and pharmacokinetic application of acetylshikonin in macaque monkeys by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). METHODS:2-Mercaptoethanol was added to the blood sample a...

journal_title：Acta pharmacologica Sinica

authors： Sun DX,Tian HF,Meng ZY,Du A,Yuan D,Gu RL,Wu ZN,Dou GF

更新日期：2008-12-01 00:00:00

abstract：AIM:To study the association between APOE polymorphisms and cerebral infarction through a case-control study among the Chinese Han population. METHODS:First-ever cerebral infarction patients (n=226) whose ages ranged from 40 to 60 years old were recruited from Department of Neurology, Zhongshan Hospital, Shanghai, and...

journal_title：Acta pharmacologica Sinica

authors： Jin ZQ,Fan YS,Ding J,Chen M,Fan W,Zhang GJ,Zhang BH,Yu SJ,Zhang YS,Ji WF,Zhang JG

更新日期：2004-03-01 00:00:00

abstract：:The fibroblast growth factor receptors (FGFRs) are increasingly considered attractive targets for therapeutic cancer intervention due to their roles in tumor metastasis and angiogenesis. Here, we identified a new selective FGFR inhibitor, C11, and assessed its antitumor activities. C11 was a selective FGFR1 inhibitor ...

journal_title：Acta pharmacologica Sinica

authors： Chen Z,Tong LJ,Tang BY,Liu HY,Wang X,Zhang T,Cao XW,Chen Y,Li HL,Qian XH,Xu YF,Xie H,Ding J

更新日期：2019-06-01 00:00:00

abstract：:Astragaloside IV (AsIV) is an active saponin extracted from Astragalus membranaceus, which has shown cardioprotective effects in a number of experimental animals. In this study we investigated the molecular mechanisms by which AsIV attenuated the myocardial ischemia reperfusion (MI/R)-induced injury in vitro and in vi...

journal_title：Acta pharmacologica Sinica

authors： Yin B,Hou XW,Lu ML

更新日期：2019-05-01 00:00:00

abstract：AIM:To investigate the influence of picroside II on hepatocyte apoptosis and its mechanism. METHODS:Morphological changes and quantification of apoptotic cells were determined under transmission electron microscopy and flow cytometry respectively. DNA fragmentation was visualized by agarose gel electrophoresis. Semi-q...

journal_title：Acta pharmacologica Sinica

authors： Gao H,Zhou YW

更新日期：2005-06-01 00:00:00

abstract：:Autophagy is a cell self-digestion process via lysosomes that clears "cellular waste", including aberrantly modified proteins or protein aggregates and damaged organelles. Therefore, autophagy is considered a protein and organelle quality control mechanism that maintains normal cellular homeostasis. Dysfunctional auto...

journal_title：Acta pharmacologica Sinica

authors： He LQ,Lu JH,Yue ZY

更新日期：2013-05-01 00:00:00

abstract：AIM:To investigate the effects of 3,4,4'-trihydroxy-trans-stilbene (3,4,4'-THS), an analogue of resveratrol, on human non-small-cell lung cancer (NSCLC) cells in vitro. METHODS:Cell viability of NSCLC A549 cells was determined by MTT assay. Cell apoptosis was evaluated using flow cytometry and TUNEL assay. Cell necros...

journal_title：Acta pharmacologica Sinica

authors： Zhang L,Dai F,Sheng PL,Chen ZQ,Xu QP,Guo YQ

更新日期：2015-10-01 00:00:00

abstract：:Aflibercept, as a soluble decoy vascular endothelial growth factor receptor, Which has been used as a first-line monotherapy for cancers. Aflibercept often causes cardiovascular toxicities including hypertension, but the mechanisms underlying aflibercept-induced hypertension remain unknown. In this study we investigat...

journal_title：Acta pharmacologica Sinica

authors： Dong ZC,Wu MM,Zhang YL,Wang QS,Liang C,Yan X,Zou LX,Chen C,Han X,Zhang B,Zhang ZR

更新日期：2020-12-10 00:00:00

abstract：AIM:To study the changes of phospholipase D (PLD) activity of actively sensitized rat peritoneal mast cells (RPMC) in degranulation. METHODS:Degranulation of RPMC was determined by measurement of beta-hexosaminidase release. PLD activity assay was carried out by measurement of PLD product, choline, with chemiluminesce...

journal_title：Acta pharmacologica Sinica

authors： Lu YB,Wu M,Zhou HL

更新日期：2004-01-01 00:00:00

abstract：AIM:alpha2 nAChR subunit mRNA expression in mice is most intense in the olfactory bulbs and interpeduncular nucleus. We aimed to investigate the properties of alpha2* nAChRs in these mouse brain regions. METHODS:alpha2 nAChR subunit-null mutant mice were engineered. Pharmacological and immunoprecipitation studies were...

journal_title：Acta pharmacologica Sinica

authors： Whiteaker P,Wilking JA,Brown RW,Brennan RJ,Collins AC,Lindstrom JM,Boulter J

更新日期：2009-06-01 00:00:00

abstract：AIM:To investigate the metabolic characteristics of cytochrome P-450 C YP2A6 in human liver microsomes. METHODS:Cytochrome P-450 enzyme activities were measured by biochemical assays. Xenobiotics were employed to observe their effects on CYP2A6 in vitro. The kinetics of coumarin 7-hydroxylase was determined, and the c...

journal_title：Acta pharmacologica Sinica

authors： Xia XY,Peng RX,Yu JP,Wang H,Wang J

更新日期：2002-05-01 00:00:00

abstract：:Recent evidence shows that high glucose levels recruit carbohydrate response element-binding protein, which binds the promoter of thioredoxin-interacting protein (txnip), thereby regulating its expression in β-cells. Overexpression of txnip not only induces β-cell apoptosis but also reduces insulin production. Thus, t...

journal_title：Acta pharmacologica Sinica

authors： Li T,Lin GY,Zhong L,Zhou Y,Wang J,Zhu Y,Feng Y,Cai XQ,Liu Q,Nosjean O,Boutin JA,Renard P,Yang DH,Wang MW

更新日期：2017-07-01 00:00:00

abstract：AIM:To examine if steroid-like compounds found in many Chinese medicinal products conventionally used for the promotion of blood circulation may act as active components via the same molecular mechanism triggered by cardiac glycosides, such as ouabain. METHODS:The inhibitory potency of ouabain and the identified stero...

journal_title：Acta pharmacologica Sinica

authors： Chen RJ,Chung TY,Li FY,Yang WH,Jinn TR,Tzen JT

更新日期：2010-06-01 00:00:00

abstract：:Anlotinib is a new oral tyrosine kinase inhibitor; this study was designed to characterize its pharmacokinetics and disposition. Anlotinib was evaluated in rats, tumor-bearing mice, and dogs and also assessed in vitro to characterize its pharmacokinetics and disposition and drug interaction potential. Samples were ana...

journal_title：Acta pharmacologica Sinica

authors： Zhong CC,Chen F,Yang JL,Jia WW,Li L,Cheng C,Du FF,Zhang SP,Xie CY,Zhang NT,Olaleye OE,Wang FQ,Xu F,Lou LG,Chen DY,Niu W,Li C

更新日期：2018-06-01 00:00:00