Effects of various principles from Chinese herbal medicine on rhodamine123 accumulation in brain capillary endothelial cells.

abstract：AIM:To examine the anti-cancer effects of chamaejasmenin B and neochamaejasmin C, two biflavonones isolated from the root of Stellera chamaejasme L (known as the traditional Chinese herb Rui Xiang Lang Du) in vitro. METHODS:Human liver carcinoma cell lines (HepG2 and SMMC-7721), a human non-small cell lung cancer cell...

journal_title：Acta pharmacologica Sinica

authors： Zhang C,Zhou SS,Feng LY,Zhang DY,Lin NM,Zhang LH,Pan JP,Wang JB,Li J

更新日期：2013-02-01 00:00:00

abstract：:Monoclonal antibodies are believed to be magic bullets and hold great potential for lots of biological process. About 100 μg of mAb109 was expressed in 5 × 106 cells after 10 days' immunization. 64Cu-NOTA-mAb109 was synthesized with the specific activity of 0.74 MBq/μg and high in vitro stability. The binding affinity...

journal_title：Acta pharmacologica Sinica

authors： Zhu H,Liu TL,Liu CH,Wang J,Zhang H,Dong B,Shen J,Zhao CK,Li ZF,Cheng Z,Yang Z

更新日期：2020-01-01 00:00:00

abstract：AIM:To determine the role of dietary low histamine on the seizure development of pentylenetetrazol (PTZ)-induced kindling in rats. METHODS:After 14 d of feeding on a low histamine diet (LH, containing 0.145 mumol/g of histamine), the rats were chemically kindled by repeated intraperitoneal injection of a subconvulsant...

journal_title：Acta pharmacologica Sinica

authors： Jin CL,Sakurai E,Kiso Y,Luo JH,Yanai K,Chen Z

更新日期：2005-04-01 00:00:00

abstract：:Abnormal growth of the intimal layer of blood vessels (neointima formation) contributes to the progression of atherosclerosis and in-stent restenosis. Recent evidence shows that the 18-kDa translocator protein (TSPO), a mitochondrial membrane protein, is involved in diverse cardiovascular diseases. In this study we in...

journal_title：Acta pharmacologica Sinica

authors： Wu LP,Gong ZF,Wang H,Zhou ZS,Zhang MM,Liu C,Ren HM,Yang J,Han Y,Zeng CY

更新日期：2020-01-01 00:00:00

abstract：:Diabetic nephropathy (DN) is characterized by sterile inflammation with continuous injury and loss of renal inherent parenchyma cells. Podocyte is an essential early injury target in DN. The injury and loss of podocytes are closely associated with proteinuria, the early symptom of renal injury in DN. However, the exac...

journal_title：Acta pharmacologica Sinica

authors： Cheng Q,Pan J,Zhou ZL,Yin F,Xie HY,Chen PP,Li JY,Zheng PQ,Zhou L,Zhang W,Liu J,Lu LM

更新日期：2020-09-23 00:00:00

abstract：:Lappaconitine (LA) has been widely used for postoperative and cancer pain control. LA exhibits excellent analgesic activity with a longer effective time than common local anesthetics such as tetracaine and bupivacaine. However, the mechanisms underlying the featured analgesic activity of LA remain largely unknown. Her...

journal_title：Acta pharmacologica Sinica

authors： Li YF,Zheng YM,Yu Y,Gan Y,Gao ZB

更新日期：2019-04-01 00:00:00

abstract：AIM:To investigate the effects of angiotensin II (Ang II) on corticotropin-releasing hormone (CRH) and argipressin (AVP) release from median eminence (ME) of hypothalamus during acute hypoxia in rats. METHODS:Simulated hypoxia was performed in a hypobaric chamber. CRH and AVP were determined by radioimmunoassay (RIA)....

journal_title：Acta pharmacologica Sinica

authors： Wu Y,Du JZ

更新日期：2000-11-01 00:00:00

abstract：AIM:To develop a high-throughput real-time assay based on molecular beacons to monitor the integrase 3'-processing reaction in vitro and apply it to inhibitor screening. METHODS:The recombinant human immunodeficiency virus (HIV)-1 integrase (IN) is incubated with a 38 mer oligonucleotide substrate, a sequence identica...

journal_title：Acta pharmacologica Sinica

authors： He HQ,Ma XH,Liu B,Zhang XY,Chen WZ,Wang CX,Cheng SH

更新日期：2007-06-01 00:00:00

abstract：AIM:To study the effects of 3-n-butylphthalide (NBP) on the TREK-1 channel expressed in Chinese hamster ovary (CHO) cells. METHODS:Whole-cell patch-clamp recording was used to record TREK-1 channel currents. The effects of varying doses of l-NBP on TREK-1 currents were also observed. Current-clamp recordings were perf...

journal_title：Acta pharmacologica Sinica

authors： Ji XC,Zhao WH,Cao DX,Shi QQ,Wang XL

更新日期：2011-02-01 00:00:00

abstract：:Cocaine is one of the most abused illicit drugs worldwide. It is well known that the dopamine (DA) transporter is its major target; but cocaine also acts on other targets including nicotinic acetylcholine receptors (nAChRs). In this study, we investigated the effects of cocaine on a special subtype of neuronal nAChR, ...

journal_title：Acta pharmacologica Sinica

authors： Ma ZG,Jiang N,Huang YB,Ma XK,Brek Eaton J,Gao M,Chang YC,Lukas RJ,Whiteaker P,Neisewander J,Wu J

更新日期：2020-02-01 00:00:00

abstract：:Herbal and dietary supplements (HDS)-induced liver injury has been a great concern all over the world. Polygonum multiflorum Thunb., a well-known Chinese herbal medicine, is recently drawn increasing attention because of its hepatotoxicity. According to the clinical and experimental studies, P. multiflorum-induced liv...

journal_title：Acta pharmacologica Sinica

authors： Rao T,Liu YT,Zeng XC,Li CP,Ou-Yang DS

更新日期：2021-01-01 00:00:00

abstract：AIM:Radiation-induced brain injury (RIBI) is the most common and severe adverse effect induced by cranial radiation therapy (CRT). In the present study, we examined the effects of the traditional Chinese medicine Shenqi Fuzheng Injection (SFI) on RIBI in mice, and explored the underlying mechanisms. METHODS:C57BL/6J m...

journal_title：Acta pharmacologica Sinica

authors： Zhang J,Tong F,Cai Q,Chen LJ,Dong JH,Wu G,Dong XR

更新日期：2015-11-01 00:00:00

abstract：:Alternative splicing is a key process of multi-exonic gene expression during pre-mRNA maturation. In this process, particular exons of a gene will be included within or excluded from the final matured mRNA, and the resulting transcripts generate diverse protein isoforms. Recent evidence demonstrates that approximately...

journal_title：Acta pharmacologica Sinica

authors： Le KQ,Prabhakar BS,Hong WJ,Li LC

更新日期：2015-10-01 00:00:00

abstract：AIM:To study the association between APOE polymorphisms and cerebral infarction through a case-control study among the Chinese Han population. METHODS:First-ever cerebral infarction patients (n=226) whose ages ranged from 40 to 60 years old were recruited from Department of Neurology, Zhongshan Hospital, Shanghai, and...

journal_title：Acta pharmacologica Sinica

authors： Jin ZQ,Fan YS,Ding J,Chen M,Fan W,Zhang GJ,Zhang BH,Yu SJ,Zhang YS,Ji WF,Zhang JG

更新日期：2004-03-01 00:00:00

abstract：AIM:To investigate the ability of an antisense RNA eukaryotic expression plasmid pcDNA3.1/survivin in down-regulating the expression level of survivin mRNA and survivin protein and reversed multidrug resistance (MDR) in adriamycin-resistant HL-60/ADR cell line. METHODS:The expression of survivin mRNA was measured by R...

journal_title：Acta pharmacologica Sinica

authors： Wang L,Zhang GM,Feng ZH

更新日期：2003-12-01 00:00:00

abstract：AIM:To investigate the effect of melittin (Mel) on papillary muscles of guinea pigs. METHODS:Contraction of papillary muscles were examined by conventional method and action potentials (AP) were recorded by standard glass microelectrode technique. RESULTS:Mel (0.5, 3 micromol/L) significantly increased the contractil...

journal_title：Acta pharmacologica Sinica

authors： Zhang YL,Yang S,Shi NC,Jiang MH

更新日期：2001-08-01 00:00:00

abstract：AIM:To investigate the mechanism of brain interleukin-1 beta (IL-1 beta) in reserpine-induced behavioral depression in rats. METHODS:Porsult swim test was used in the measurement of depressive behavior and ELISA was used in measurement of brain IL-1 beta. RESULTS:Intraperitoneal injection of reserpine (0, 4, 6, and 8...

journal_title：Acta pharmacologica Sinica

authors： Huang QJ,Jiang H,Hao XL,Minor TR

更新日期：2004-03-01 00:00:00

abstract：AIM:To evaluation the doxorubicin (DOX)-loaded pH-sensitive polymeric micelle release from tumor blood vessels into tumor interstitium using an animal vessel visibility model, the so-called dorsal skin-fold window chamber model. METHODS:DOX-loaded pH-sensitive polyHis-b-PEG micelles and DOX-loaded pH-insensitive PLLA-...

journal_title：Acta pharmacologica Sinica

authors： Jin ZH,Jin MJ,Jiang CG,Yin XZ,Jin SX,Quan XQ,Gao ZG

更新日期：2014-06-01 00:00:00

abstract：AIM:To develop a robust, cell-based, high-content screening (HCS) assay based on receptor internalization for the identification of novel modulators of the epidermal growth factor receptor (EGFR). METHODS:Agonist-induced receptor internalization is part of the signaling cascade of EGFR. Fluorescent-tagged epidermal gr...

journal_title：Acta pharmacologica Sinica

authors： Wang J,Xie X

更新日期：2007-10-01 00:00:00

abstract：AIM:To express dopamine D1 receptor in baculovirus-Sf9 cell system, and to investigate the effects of l-12-chloroscoulerine (l-CSL) on the recombinant D1 receptor (D1R). METHODS:The recombinant baculovirus, Autographa californica nuclear polyhedrosis virus bearing D1R (AcNPV- D1R) was generated, and then was used to p...

journal_title：Acta pharmacologica Sinica

authors： He Y,Jin WQ,Shen QX,Chen XJ,Jin GZ

更新日期：2003-03-01 00:00:00

abstract：:Aflibercept, as a soluble decoy vascular endothelial growth factor receptor, Which has been used as a first-line monotherapy for cancers. Aflibercept often causes cardiovascular toxicities including hypertension, but the mechanisms underlying aflibercept-induced hypertension remain unknown. In this study we investigat...

journal_title：Acta pharmacologica Sinica

authors： Dong ZC,Wu MM,Zhang YL,Wang QS,Liang C,Yan X,Zou LX,Chen C,Han X,Zhang B,Zhang ZR

更新日期：2020-12-10 00:00:00

abstract：:In an effort to develop effective alternative strategies that increase the therapeutic efficacy and minimize the systemic toxicity of chemotherapeutic agents, more efforts are being directed towards the investigation of dietary supplements and other phytotherapeutic agents for their synergistic efficacy in combination...

journal_title：Acta pharmacologica Sinica

authors： Raina K,Agarwal R

更新日期：2007-09-01 00:00:00

abstract：AIM:To investigate the effects of onychin on the proliferation of cultured rat artery vascular smooth muscle cells (VSMCs) in the presence of 10% new-born calf serum (NCS). METHODS:Rat VSMCs were incubated with onychin 150 micromol/L or genistein 10 micromol/L in the presence of 10% NCS for 24 h. The proliferation of ...

journal_title：Acta pharmacologica Sinica

authors： Yang M,Huang HL,Zhu BY,Tuo QH,Liao DF

更新日期：2005-02-01 00:00:00

abstract：:It was reported that antituberculosis medicines could induce liver damage via oxidative stress. In this study, we investigated the effects of rifampicin (RFP) on the membrane expression of multidrug resistance-associated protein 2 (MRP2) and the relationship between oxidative stress and RFP-induced endocytosis of MRP2...

journal_title：Acta pharmacologica Sinica

authors： Xu BY,Tang XD,Chen J,Wu HB,Chen WS,Chen L

更新日期：2020-01-01 00:00:00

abstract：:Cysteine-rich peptides from the venom of cone snails (Conus) target a wide variety of different ion channels. One family of conopeptides, the alpha-conotoxins, specifically target different isoforms of nicotinic acetylcholine receptors (nAChRs) found both in the neuromuscular junction and central nervous system. This ...

journal_title：Acta pharmacologica Sinica

authors： Azam L,McIntosh JM

更新日期：2009-06-01 00:00:00

abstract：:Embryonic stem (ES) cells and their differentiated progeny offer tremendous potential for regenerative medicine, even in the field of drug discovery. There is an urgent need for clinically relevant assays that make use of ES cells because of their rich biological utility. Attention has been focused on small molecules ...

journal_title：Acta pharmacologica Sinica

authors： Lou YJ,Liang XG

更新日期：2011-02-01 00:00:00

abstract：:Human T-cell lymphotropic virus type 1 (HTLV-1) is the etiologic agent of adult T-cell leukemia/lymphoma (ATL), a rapidly progressing clonal malignancy of CD4+ T lymphocytes. Exploring the host-HTLV-1 interactions and the molecular mechanisms underlying HTLV-1-mediated tumorigenesis is critical for developing efficien...

journal_title：Acta pharmacologica Sinica

authors： Zhang LL,Wei JY,Wang L,Huang SL,Chen JL

更新日期：2017-08-01 00:00:00

abstract：:Etimicin (ETM), a fourth-generation aminoglycosides (AGs), is now widely clinically used in China due to its high efficacy and low toxicity. However, the mechanisms underlying its low nephrotoxicity and ototoxicity remain unclear. In the present study we compared the antibacterial and toxicity profiles of etimicin, ge...

journal_title：Acta pharmacologica Sinica

authors： Yao L,Zhang JW,Chen B,Cai MM,Feng D,Wang QZ,Wang XY,Sun JG,Zheng YW,Wang GJ,Zhou F

更新日期：2020-06-01 00:00:00

abstract：AIM:To investigate the effects of resveratrol on interleukin-1beta (IL-1beta) induced catabolism in bone marrow mesenchymal stem cell (MSC) derived chondrocytes cultured on chitosan-gelatin scaffolds (CGS). METHODS:The chondrogenesis of alginate-encapsulated MSCs was evaluated by toluidine blue staining, RT-PCR, and i...

journal_title：Acta pharmacologica Sinica

authors： Lei M,Liu SQ,Liu YL

更新日期：2008-11-01 00:00:00

abstract：AIM:Based on the structural analysis to reveal the mechanism of ligand binding to beta-secretase and the specificity of each binding sub-site. METHODS:Molecular dynamics was used to simulate on the ligand free beta-secretase and ligand bound beta-secretase. The trajectories were analyzed using the essential dynamics, ...

journal_title：Acta pharmacologica Sinica

authors： Xiong B,Huang XQ,Shen LL,Shen JH,Luo XM,Shen X,Jiang HL,Chen KX

更新日期：2004-06-01 00:00:00