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Expression of angiopoietin-2 and vascular endothelial growth factor in mice cerebral cortex after permanent focal cerebral ischemia.

Abstract：

AIM:To study the expressions of vascular endothelial growth factor (VEG F), angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2), Tie-1, and Tie-2 in C57BL/6 mouse brain after permanent focal cerebral ischemia. METHODS:The mRNA levels of VEGF, Ang-1, Ang-2, Tie-1, and Tie-2 were measured by semiquantitative reverse transcription polymerase chain reaction (RT-PCR). The protein express ions of VEGF and Ang-2 were determined by immunohistochemistry. RESULTS:Low mRNA levels of VEGF, Ang-1, Ang-2, Tie-1, and Tie-2 were constitutively expressed in the normal cortex of mouse. After middle cerebral artery occlusion (MCAO), the expressions of VEGF, Ang-2, and Tie-2 mRNA were dramatically increased in the infarcted cortex and the elevation was remained through 7 d of ischemia. However, the levels of Ang-1 and Tie-1 mRNA were unchanged in the infarcted cortex. Immunoreactivities of Ang-2 or VEGF were hardly observed in the normal cortex. Ang-2 protein was evidently detected in the infarct core 8 h after MCAO and in t he perifocal area 1 d after MCAO. Expression of VEGF protein was elevated in the infarct core 2 h after MCAO and in the perifocal area 1 d after MCAO. Immunoreaction was restricted to endothelial cells and glial-like cells within the infarct core and perifocal area. CONCLUSION:The expressions of An g-2 and VEGF are induced after focal cerebral ischemia, which may contribute to the angiogenic response in the cortex of ischemic brain.

journal_name

Acta Pharmacol Sin

journal_title

Acta pharmacologica Sinica

authors

Wang RG,Zhu XZ

subject

Has Abstract

pub_date

2002-05-01 00:00:00

pages

405-11

issue

5

eissn

1671-4083

issn

1745-7254

journal_volume

23

pub_type

杂志文章

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