当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Different pathways of inhibitory effects of wortmannin on exocytosis are revealed by video-enhanced light microscope.

Different pathways of inhibitory effects of wortmannin on exocytosis are revealed by video-enhanced light microscope.

Abstract：

:Exocytosis of secretory granules, including histamine, in rat basophilic leukemia (RBL-2H3) cells, which exhibit Ca(2+)-dependent secretion of granules when stimulated with antigen or Ca(2+)-ionophore (A23187), was observed under a video-enhanced light microscope. Exocytotic events of individual granules including fusion, extrusion, and membrane retrieval were visualized in individual cells stimulated with antigen or A23187. Exocytosis of granules stimulated with A23187 showed two peaks in its time courses. The earlier one of the peaks was inhibited by wortmannin ( > 100 nM), as an inhibitor of myosin light chain kinase (MLCK), and the other was not. Exocytosis by antigen-stimulation, however, showed only one peak, which was inhibited by low concentration of wortmannin ( < 50 nM) as an inhibitor of phosphatidylinositol 3-kinase (PI3-kinase). These results indicate that quantitative analysis of exocytosis visualized by video-enhanced light microscope reveals two different pathways, through P13-kinase and MLCK, of inhibitory effects on exocytosis by wortmannin in RBL-2H3 cells.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Ozawa K,Masujima T,Ikeda K,Kodama Y,Nonomura Y

doi

10.1006/bbrc.1996.0729

subject

Has Abstract

pub_date

1996-05-15 00:00:00

pages

243-8

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(96)90729-9

journal_volume

222

pub_type

杂志文章

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