Abstract：
PURPOSE:To determine whether alterations of p53, a tumor suppressor gene, were present in uveal melanoma, and to characterize further the nature of those changes. METHODS:Immunohistochemical analysis with a monoclonal antibody was used to determine whether alterations of p53 were present in 35 enucleated archival uveal melanomas. Further characterization was done by comparing the p53 gene and cell cycling status by using bromodeoxyuridine staining. The alterations in p53 were characterized using polymerase chain reaction single-strand conformational polymorphism analysis and sequencing. RESULTS:Four of 35 uveal melanomas showed low levels (0.5% to 5.0%) of positive immunostaining for altered p53 in tumor cell nuclei using monoclonal antibody DO-7. These four tumors had the three highest and the 14th highest bromodeoxyuridine labeling indices, ranging from 1.3% to 7.0%. Polymerase chain reaction single-strand conformational polymorphism analysis of p53 exons 5 to 8 was performed on three p53-positive and six p53-negative tumors, and no altered motility shifts were detected. Sequencing of one of the positive staining specimens confirmed no mutations in exons 5 through 8 in the p53 gene. Double immunohistochemical labeling for both bromodeoxyuridine and p53 in one tumor showed that most of p53-positive cells were in S phase. CONCLUSIONS:Mutation of p53 is an uncommon event in uveal melanomas. Nuclear accumulation of p53 protein was found in three of the four tumors with the highest levels of cell cycling.
journal_name
Am J Ophthalmoljournal_title
American journal of ophthalmologyauthors
Kishore K,Ghazvini S,Char DH,Kroll S,Selle Jdoi
10.1016/s0002-9394(14)75431-5subject
Has Abstractpub_date
1996-05-01 00:00:00pages
561-7issue
5eissn
0002-9394issn
1879-1891pii
S0002-9394(14)75431-5journal_volume
121pub_type
杂志文章abstract：:Amyloid fibrils occurring in primary and myeloma-associated (AL), secondary (AA), and certain neuropathic hereditary forms of systemic amyloidosis can be distinguished biochemically or immunohistologically as being composed of immunoglobulin light chain, protein AA, or prealbumin respectively. All types of systemic an...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(87)90357-6
更新日期：1984-08-15 00:00:00
abstract：PURPOSE:We sought to assess the effects of exposure to secondhand smoke (SHS) on peripapillary retinal nerve fiber layer (p-RNFL) thickness in children. DESIGN:Cross-sectional study. METHODS:Children 6-8 years of age were consecutively recruited from the population-based Hong Kong Children Eye Study. All participants...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2020.10.016
更新日期：2020-10-29 00:00:00
abstract：:A 62-year-old woman developed a recurrent mucinous sweat gland adenocarcinoma of the eyelid. The clinical characteristics of this rare, primary adnexal neoplasm of skin in our patient were, to our knowledge, unique. The tumor mimicked a lacrimal sac tumor and had orbital extension demonstrated preoperatively by comput...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(81)90767-4
更新日期：1981-08-01 00:00:00
abstract：PURPOSE:To assess the optic nerve head (ONH) by optical coherence tomography (OCT), confocal scanning laser ophthalmoscopy (CSLO), and the retinal nerve fiber layer (RNFL) by OCT and scanning laser polarimetry (GDx) in highly myopic subjects. DESIGN:Observational cross-sectional study. METHODS:Thirty-five eyes of hig...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2006.05.022
更新日期：2006-11-01 00:00:00
abstract：:A 20-month-old boy with a ciliary body tumor presented with two white flocculi floating in the anterior chamber of his left eye. This material was examined by electron microscopy. Both the clinical appearance of the tumor and the ultrastructural findings suggested the diagnosis of medulloepithelioma. The flocculi cont...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(75)90089-6
更新日期：1975-02-01 00:00:00
abstract：PURPOSE:To assess the accuracy with which the Keratron keratoscope (Optikon 2000, Rome, Italy) measured astigmatic test surfaces by a profile reconstruction algorithm within a plane geometry model and to discriminate between error caused by the model and error caused by other factors. METHODS:Height was reported by th...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(14)70633-6
更新日期：1996-06-01 00:00:00
abstract：PURPOSE:Congenital macular lesions attributed to toxoplasmosis may limit potential visual acuity. The appearance and location of these scars may cause physicians to overlook associated amblyopia. This study reviews the visual outcomes and benefits of amblyopia therapy in children with foveal toxoplasmosis scars. DESIG...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2020.01.034
更新日期：2020-06-01 00:00:00
abstract：PURPOSE:To describe a case of bone marrow transplantation retinopathy in a patient in the absence of prior radiation therapy. METHODS:Case report. RESULTS:Bilateral scotomata developed in a 26-year-old woman because of a retinal microvasculopathy two years after receiving an autologous bone marrow transplantation for...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(14)72023-9
更新日期：1996-08-01 00:00:00
abstract：PURPOSE:To characterize perifoveal intraretinal cavities observed around full-thickness macular holes (MH) using en face optical coherence tomography and to establish correlations with histology of human and primate maculae. DESIGN:Retrospective nonconsecutive observational case series. METHODS:Macular en face scans ...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2015.02.013
更新日期：2015-06-01 00:00:00
abstract：PURPOSE:To report the results and complications of an aspiration cutter-assisted anterior segment tumor biopsy performed under viscoelastic. DESIGN:Retrospective, consecutive, interventional case series. METHODS:Fifty-five patients with anterior segment tumors underwent aspiration cutter-assisted biopsy at a single c...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2011.04.032
更新日期：2011-11-01 00:00:00
abstract：PURPOSE:To report a patient with uveal effusion and intermittent angle-closure glaucoma associated with primary pulmonary hypertension. METHODS:Observational case report. RESULTS:A 78-year-old woman with primary pulmonary hypertension noticed pain and decreased vision in her right eye. Ocular examination disclosed ex...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(02)02090-1
更新日期：2003-05-01 00:00:00
abstract：PURPOSE:To report a case of porphyria cutanea tarda presenting as cicatricial conjunctivitis. DESIGN:Observational study. METHODS:A 31-year-old man presented with bilateral inferior symblepharon, superior tarsal conjunctival scarring and concretions, and recurrent conjunctival and episcleral injection. RESULTS:Four ...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(02)01623-9
更新日期：2002-10-01 00:00:00
abstract：:A 14-year-old boy was stabbed above the left eye and had subsequent loss of both retinal and choroidal perfusion to the posterior segment. Ophthalmoscopic examination ten days after the injury disclosed intense nerve fiber layer edema, most prominent in the posterior pole, and absence of a cherry-red spot. Intravenous...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(79)90666-4
更新日期：1979-10-01 00:00:00
abstract：PURPOSE:In previous studies of fungal keratitis (FK) from temperate countries, yeasts were the predominant isolates, with ocular surface disease (OSD) being the leading risk factor. Since the 2005-2006 outbreak of contact lens (CL)-associated Fusarium keratitis, there may have been a rise in CL-associated filamentary F...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2016.05.021
更新日期：2016-08-01 00:00:00
abstract：:Forty-three patients who initially presented with Krikenberg's spindles but with no glaucomatous field defects were observed for an average of 5.8 years in an attempt to evaluate the prognostic implication of spindle presence. Twenty-two of the patients had been followed for a minimum of five years. Analysis of the st...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(75)90075-6
更新日期：1975-02-01 00:00:00
abstract：PURPOSE:To determine the morphologic changes in human corneas over time following radiofrequency-based conductive keratoplasty (CK) treatment. DESIGN:Prospective, observational case series. METHODS:In a single-center study six human corneas of six patients with localized peripheral keratoconus underwent CK treatment ...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2005.06.027
更新日期：2005-12-01 00:00:00
abstract：:We performed visual contrast sensitivity testing with Regan's low-contrast letter charts on 30 patients with optic neuropathies and good Snellen visual acuity. Results of this testing were compared with color vision and the presence of an afferent pupillary defect. Regan's low-contrast letter charts were found to be 9...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(88)90176-6
更新日期：1988-02-15 00:00:00
abstract：:In a postmortem study of 57 diabetic patients, 19 of 23 patients with blood glucose levels greater than 200 mg/100 ml in the 72-hour period before death showed the characteristic histologic findings of "lacy" vacuolation of the iris pigment epithelium as well as vacuolation of the renal tubules (Armanni-Ebstein nephro...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(75)90749-7
更新日期：1975-05-01 00:00:00
abstract：PURPOSE:To report the use of optical coherence tomography (OCT) for evaluation of diffuse diabetic macular edema (DME) before and after vitrectomy. DESIGN:Interventional case series. METHODS:A retrospective study was made of 15 consecutive eyes of 13 patients that had vitrectomy for diffuse DME and OCT preoperatively...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(02)01837-8
更新日期：2003-02-01 00:00:00
abstract：PURPOSE:To report a patient with ocular ischemic syndrome due to an internal carotid artery stenosis in whom we confirmed improved retinal blood flow noninvasively after carotid endarterectomy. DESIGN:Observational case report. METHODS:Retinal flowmetry. RESULTS:In a 72-year-old hypertensive man with a transient isc...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(02)01531-3
更新日期：2002-08-01 00:00:00
abstract：PURPOSE:To report the successful use of topical cysteamine formulated from the oral preparation in the treatment of severe photophobia from corneal crystal deposition in cystinosis. DESIGN:Interventional case report. METHODS:Retrospective chart review. RESULTS:An 8-year-old boy with nephropathic cystinosis was exper...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2004.05.005
更新日期：2004-10-01 00:00:00
abstract：:We investigated albumin and IgG concentrations in the aqueous humor of 41 patients before intracapsular cataract extraction (primary samples) and at the end of surgery (secondary samples). Albumin and IgG concentrations were determined in all samples by means of a quantitative fluoroimmunoassay. A short-term topical a...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(14)71914-2
更新日期：1983-12-01 00:00:00
abstract：:A patient who used contact lenses and had a history of blunt trauma developed vaccinia keratouveitis after accidental ocular autoinoculation from a recent vaccination site. Corneal and conjunctival cultures were taken for bacteria, fungi, Acanthamoeba, and viruses. Viral-like cytopathic effects became evident in tissu...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(14)70008-x
更新日期：1994-04-15 00:00:00
abstract：PURPOSE:To assess the potential effect of intravitreal bevacizumab (IVB) (Avastin) on retinal function and anatomic recovery in eyes with choroidal neovascularization attributable to pathological myopia (mCNV). DESIGN:Retrospective case series. METHODS:setting: Institutional. patient population: Sixty-three eyes of 6...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2008.07.017
更新日期：2009-01-01 00:00:00
abstract：PURPOSE:To investigate long-term responsiveness of patients with severe uveitis associated with Behçet disease to interferon alpha2a (IFN-alpha2a). DESIGN:Retrospective study. METHODS:Thirty-two patients with sight-threatening uveitis associated with Behçet disease who relapsed despite corticosteroids and immunosuppr...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2008.08.038
更新日期：2008-12-01 00:00:00
abstract：PURPOSE:To determine whether central fusion and distance stereoacuity are useful as objective measures in assessing the need for and success of surgery for intermittent exotropia (X[T]). METHODS:A prospective, institutional, clinical trial was conducted of 26 consecutive patients with X(T) who were undergoing strabism...
journal_title：American journal of ophthalmology
pub_type： 临床试验,杂志文章
doi：10.1016/s0002-9394(99)00079-3
更新日期：1999-08-01 00:00:00
abstract：:We measured lower eyelid length in 288 healthy subjects to establish a norm and then in 95 patients with complaints of intractable photophobia, foreign-body sensation, chronic tearing, or chronic recurrent conjunctivitis. The difference in length was statistically significant (34.25 +/- 2.43 mm for healthy men vs 36.5...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(83)90279-9
更新日期：1983-04-01 00:00:00
abstract：PURPOSE:To study the glistening formation in various hydrophobic-acrylic intraocular lens (IOL) models, and to evaluate the effect of glistenings on light scattering in these IOLs. DESIGN:Laboratory investigation. METHODS:The susceptibility of the hydrophobic-acrylic material to develop glistenings was evaluated in 6...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2018.08.032
更新日期：2018-12-01 00:00:00
abstract：PURPOSE:To assess trends in prevalence of diagnosed ocular disease and use of eye care services in the Veterans Affairs (VA) health care system. DESIGN:Prevalence study. METHODS:We performed a retrospective study of all eligible veterans in the VA Capitol Health Care Network from 2007 to 2011. The VA database was use...
journal_title：American journal of ophthalmology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.ajo.2016.09.030
更新日期：2017-01-01 00:00:00
abstract：PURPOSE:To examine the influence of corneal allograft rejection on the survival of penetrating corneal transplantation, to review the status of conventional therapies to improve graft survival, and to consider prospects for alternative approaches to reduce the impact of rejection. DESIGN:Perspective, including prospec...
journal_title：American journal of ophthalmology
pub_type： 杂志文章,评审
doi：10.1016/j.ajo.2005.07.024
更新日期：2005-12-01 00:00:00
