Abstract：
:We examined the relationship between p53 levels before and after irradiation, radiation-induced cell cycle delays, apoptotic cell death and radiosensitivity in a panel of eight human tumour cell lines. The cell lines differed widely in their clonogenic survival after radiation, (surviving fraction at 2 Gy: SF2=0.18-0.82). Constitutive p53 protein levels varied from 2.2 +/- 0.4 to 6.3 +/- 0.3 optical density units (OD) per 10(6) cells. p53 after irradiation (6 Gy) also varied between the cell lines, ranging from no induction to a 1.6-fold increase in p53 levels 4 h after treatment. p53 function was also assessed by G1 cell cycle arrest after irradiation. The cellular response to radiation, measured as G0/G1 arrest, and the induction of apoptosis were in good agreement. However, a trace amount of DNA ladder formation was found in two cell lines lacking G1 arrest. Overall cellular radiosensitivity correlated well with the level of radiation-induced G1 arrest (correlation coefficient r=0.856; P=0.0067), with p53 constitutive levels (r=0.874, P=0.0046), and with p53 protein fold induction (r=-0.882, P=0.0038). Our data suggest that (1) the constitutive p53 level, (2) G1 arrest after irradiation, or (3) the p53 protein response to radiation may be good predictive tests for radiosensitivity in some cell types.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Siles E,Villalobos M,Valenzuela MT,Núñez MI,Gordon A,McMillan TJ,Pedraza V,Ruiz de Almodóvar JMdoi
10.1038/bjc.1996.101subject
Has Abstractpub_date
1996-03-01 00:00:00pages
581-8issue
5eissn
0007-0920issn
1532-1827journal_volume
73pub_type
杂志文章abstract：:The normal-lymphocyte-transfer test has been advocated as a method of assessing the immuno-competence of lymphocytes from patients with breast and large-bowel cancer. Evidence is presented in this paper that the methodology is subject to many uncontrollable errors, that the reaction is the result of multiple factors, ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1978.219
更新日期：1978-09-01 00:00:00
abstract：:The 62 kDa protein product of the c-myc oncogene (p62 c-myc) is thought to be involved in the control of normal cellular proliferation and differentiation. We have measured oncoprotein levels using a flow cytometric assay in 141 operable breast cancers and have correlated levels with prognostic variables, patient surv...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1989.337
更新日期：1989-11-01 00:00:00
abstract：:Comfrey is a rat liver toxin and carcinogen that has been used as a vegetable and herbal remedy by humans. In order to evaluate the mechanisms underlying its carcinogenicity, we examined the mutagenicity of comfrey in the transgenic Big Blue rat model. Our results indicate that comfrey is mutagenic in rat liver and th...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6602420
更新日期：2005-03-14 00:00:00
abstract：:The acute toxicity, pharmacokinetics and hypoxic cytotoxicity of RSU-1069 were investigated using the subcutaneous (sc) rat 9L tumour model. The pharmacokinetics were studied after i.p. injection of RSU-1069 (20 mg kg-1 or 100 mg kg-1). For both doses, the elimination of RSU-1069 followed first-order kinetics in both ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1991.116
更新日期：1991-04-01 00:00:00
abstract：:HuR, a ubiquitously expressed member of the Hu protein family that binds and stabilizes an AU-rich element (ARE)-containing mRNAs, is known to shuttle between the nucleus and the cytoplasm via several export pathways. When normal cells were treated with heat shock, HuR was exported to the cytoplasm in a chromosome mai...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6605084
更新日期：2009-06-16 00:00:00
abstract：BACKGROUND:In the coBRIM study, cobimetinib plus vemurafenib (C+V) significantly improved survival outcomes vs placebo and vemurafenib (P+V) in patients with advanced/metastatic BRAFV600-mutated melanoma. An analysis of health-related quality of life (HRQOL) from coBRIM is reported. METHODS:Patients completing the Eur...
journal_title：British journal of cancer
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1038/bjc.2017.488
更新日期：2018-03-20 00:00:00
abstract：:This single-centre phase I trial was designed to determine the maximum tolerated dose of irinotecan and the recommended dose to use in combination with a fixed dose of 5-fluorouracil (5-FU) administered as a protracted venous infusion, for the first-line treatment of metastatic colorectal cancer (CRC). Tolerability an...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章
doi：10.1038/sj.bjc.6602173
更新日期：2004-10-18 00:00:00
abstract：:The author Sara Lonardi was incorrectly listed as Sara Leonardi. The correct name is listed above. ...
journal_title：British journal of cancer
pub_type： 杂志文章,已发布勘误
doi：10.1038/s41416-018-0067-0
更新日期：2018-06-01 00:00:00
abstract：BACKGROUND:The integrin-binding protein osteopontin is strongly associated with tumour development, yet is an abundant dietary component as a constituent of human and bovine milk. Therefore, we tested the effect of orally administered osteopontin (o-OPN) on the development of subcutaneous tumours in mice. METHODS:Bovi...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2014.10
更新日期：2014-03-04 00:00:00
abstract：BACKGROUND:Multiple early gastric cancers (EGCs) may develop in 6-14% of patients even after achieving curative endoscopic submucosal dissection (ESD); however, a useful biomarker for predicting recurrence is not available. The present study investigated whether the expression of CD44 variant 9 (CD44v9), a functional c...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.314
更新日期：2013-07-23 00:00:00
abstract：:Increased 2'-deoxy-2'-[18F]fluoro-D-glucose (FDG) uptake is the most commonly used marker for positron emission tomography in oncology. However, a proliferation tracer such as 3'-deoxy-3'-[18F]fluorothymidine (FLT) might be more specific for cancer. 3'-deoxy-3'-[18F]fluorothymidine uptake is dependent on thymidine kin...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604523
更新日期：2008-08-05 00:00:00
abstract：:Solid tumours are often highly acidic compared to normal tissue, and tumour extracellular acidosis contributes to multiple aspects of cancer progression. Now, Anemone et al. in this issue of the British Journal of Cancer provide in vivo evidence that the degree to which various breast cancer cell lines acidify their e...
journal_title：British journal of cancer
pub_type： 社论
doi：10.1038/s41416-020-01171-2
更新日期：2021-01-01 00:00:00
abstract：:The growth of s.c. Walker 256 carcinoma was found to be independent of secondary growths induced by i.v. injection. Tumour cells injected i.v. lodged mainly in the lungs, with small clusters of cells in the lymph nodes. The rate of cellular proliferation of these secondary growths of Walker carcinoma was significantly...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1978.12
更新日期：1978-01-01 00:00:00
abstract：:Two hundred and thirty five patients with osteosarcoma, aged less than 40 years, and treated by amputation or radiotherapy, were entered in a randomised trial of two forms of adjuvant chemotherapy. A two drug regimen, namely vincristine 2 mg m-2 (maximum 2mg) followed by methotrexate 200 mgm-2, given every three weeks...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1038/bjc.1986.81
更新日期：1986-04-01 00:00:00
abstract：:Protein kinase C (PKC) has a critical role in several signal transduction pathways, and is involved in renal cancer pathogenesis. Bryostatin-1 modulates PKC activity and has antitumour effects in preclinical studies. We conducted a multicentre phase II clinical trial in patients with advanced renal cancer to determine...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1038/sj.bjc.6601321
更新日期：2003-10-20 00:00:00
abstract：BACKGROUND:We evaluated the association between smoking status and thyroid cancer risk and whether this association is mediated by body mass index (BMI) and thyroid-stimulating hormone (TSH). METHODS:We performed a cohort study of 96,855 Korean adults who were followed annually or biennially for a median of 5.9 years....
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/s41416-018-0224-5
更新日期：2018-08-01 00:00:00
abstract：:Oestrogen receptor protein (ER) was detected in 9 of 11 samples of malignant breast tissue and 8 of 9 samples of normal breast tissue. Levels of cytosolic ER (ERc) in malignant breast were 21-1102 fmol mg-1 soluble protein (Kd 1.8 X 10(-9)-3.1 X 10(-8) mol l-1) and those of nucleosolic ER (ERn), 13-526 fmol mg-1 solub...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1986.196
更新日期：1986-09-01 00:00:00
abstract：BACKGROUND:Systemic inflammatory response (SIR) is important in the relationship between the tumour, the host, and outcome in cancer patients. However, limited data exist regarding the prognostic significance of SIR in bladder cancer. We investigate the utility of pretreatment SIR in patients with urothelial carcinoma ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2014.631
更新日期：2015-02-03 00:00:00
abstract：:Fifty-two previously untreated patients with advanced non-small-cell lung cancer (NSCLC) were treated on a 14 day cycle with cisplatin (60 mg m-2 i.v.) and vindesine (3 mg m-2 i.v.) on day 1, followed by a 3 day continuous infusion of 5-fluorouracil (800 mg m-2 day-1) starting on day 8. An overall response rate of 40....
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章
doi：10.1038/bjc.1996.211
更新日期：1996-05-01 00:00:00
abstract：:Aminoglutethimide without glucocorticoid has been shown to be a clinically effective treatment for postmenopausal breast cancer in low dosage (250 mg day-1). The mechanism of action of this approach is thought to be the inhibition of peripheral aromatase, the enzyme which converts androstenedione to oestrone. The acti...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1985.144
更新日期：1985-07-01 00:00:00
abstract：BACKGROUND:Patients whose colorectal cancer is treated after an emergency admission tend to have late-stage cancer and a poor prognosis. We identified risk factors for an emergency admission by linking data from the National Bowel Cancer Audit (NBCA) and the English Hospital Episode Statistics (HES), an administrative ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2014.300
更新日期：2014-07-29 00:00:00
abstract：:The in vitro deamination, cytotoxicity, cellular drug uptake, distribution and cellular pharmacology in HL-60 cells of N4-hexadecyl-1-beta-D-arabinofuranosylcytosine (NHAC), a lipophilic derivative of arabinofuranosylcytosine (ara-C), were studied. Compared with ara-C, NHAC in liposomal formulations was highly resista...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1995.185
更新日期：1995-05-01 00:00:00
abstract：:Our recent genome-wide allelotyping analysis of gallbladder carcinoma identified 3p, 8p, 9q and 22q as chromosomal regions with frequent loss of heterozygosity. The present study was undertaken to more precisely identify the presence and location of regions of frequent allele loss involving those chromosomes in gallbl...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600490
更新日期：2002-08-12 00:00:00
abstract：:The level of expression of the multidrug resistance-associated protein (MRP1) in a panel of human ovarian carcinoma cell lines and their variants with acquired cisplatin resistance was determined using Western blotting. No overexpression of MRP1 was detected in any of the cell lines. In addition, we have transfected t...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1998.461
更新日期：1998-07-01 00:00:00
abstract：:Pancreatic tumours are scirrhous, avascular tumours, suggesting that they may produce angiogenesis inhibitors that suppress the growth of the vasculature to the tumour and metastases. We have sought evidence for the angiogenesis inhibitor, endostatin, in normal and cancerous pancreatic tissue. Using Western blotting, ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6602234
更新日期：2005-01-17 00:00:00
abstract：BACKGROUND:Thymidine phosphorylase (TP) is often overexpressed in tumours and has a role in tumour aggressiveness and angiogenesis. Here, we determined whether TP increased tumour invasion and whether TP-expressing cancer cells stimulated angiogenesis. METHODS:Angiogenesis was studied by exposing endothelial cells (HU...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2011.74
更新日期：2011-03-29 00:00:00
abstract：:Differentiation-inducing ability of gamma-radiation, N,N-dimethylformamide and their combination has been tested on human rhabdomyosarcoma RMZ-RC2 clone cells. Ionising radiation at 2-5 Gy doses induced a more differentiated morphology, with the appearance of an increased proportion of multinuclear myotube-like cells,...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1992.107
更新日期：1992-04-01 00:00:00
abstract：:Hybrid Capture II (HC-II) is a commercial human papillomavirus (HPV) detection test designed to detect 18 HPV types divided into high-risk and low-risk groups. We have tested 1647 scrapes from 1518 unselected women attending routine cytological screening by this assay for the detection of histologically proven high-gr...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6690523
更新日期：1999-07-01 00:00:00
abstract：:Imatinib mesilate (Glivec), Novartis Pharmaceuticals) is a novel therapy for the treatment of chronic myeloid leukaemia (CML). We evaluated the cost-effectiveness of imatinib (600 mg daily) when used for the treatment of patients in advanced stages of CML (accelerated phase and blast crisis) against conventional thera...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601151
更新日期：2003-08-18 00:00:00
abstract：BACKGROUND:A major molecular pathway of genetic instability in cancer is DNA mismatch repair deficiency. High-level microsatellite instability (MSI-H) is currently the best predictor of responsiveness towards immune checkpoint blockade. Data about the prevalence of high-level microsatellite instability in cholangiocarc...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/s41416-018-0199-2
更新日期：2019-01-01 00:00:00
