Abstract：
:DNA methylation is known to be related to the regulation of gene expression. DNA methylation patterns are modified in tumors compared to normal tissue, and in some cases, these variations are linked to cancer progression. Molecular biology techniques are generally used to evaluate DNA methylation status. We describe here a simple and fast immunofluorescent method to quantitate in situ DNA methylation using an image analyser and a CCD camera. Quantification of relative methylation levels in interphase nuclei and metaphase chromosomes was performed on digital images of two types of cells with known methylation levels. The results from image cytometry corresponded to those obtained by molecular studies. Advantages of this approach are that all the DNA of a cell may be examined rather than a limited restriction sequence, and that it may be done on a cell by cell basis rather than on a heterogenous population. In addition, with this method, changes in methylation patterns during tumor progression could be followed and eventually used as a marker for prognosis.
journal_name
Bull Cancerjournal_title
Bulletin du cancerauthors
Veilleux C,Bernardino J,Gibaud A,Niveleau A,Malfoy B,Dutrillaux B,Bourgeois CAsubject
Has Abstractpub_date
1995-11-01 00:00:00pages
939-45issue
11eissn
0007-4551issn
1769-6917pii
0007-4551(96)80024-5journal_volume
82pub_type
杂志文章abstract：BACKGROUND:Depression is a sustainable state of deep sadness and abatement. It is frequently identified in patients with cancer and often undervalued by oncologists. The reduced PRIME-MD questionnaire is a questionnaire of 2 items validated in French. However, it has been neither translated nor validated in French. The...
journal_title：Bulletin du cancer
pub_type： 杂志文章
doi：10.1016/j.bulcan.2015.09.008
更新日期：2016-02-01 00:00:00
abstract：:The evidence that genetic factors are of some importance in the etiology of cancer originates has been determined by genetic epidemiology, molecular biology, cytogenetics. Genetic counselling is one of the possible strategies for controlling cancer through genetics. Genetic counselling in this field more often concern...
journal_title：Bulletin du cancer
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Since the 1980's, women treated for breast cancer are reporting moderate cognitive impairments during and after chemotherapy. These cognitive impairments may be related to chemotherapy but also to hormone therapy, psychological distress, fatigue... This work is a critical review of the literature, which reports types ...
journal_title：Bulletin du cancer
pub_type： 杂志文章,评审
doi：10.1684/bdc.2008.0818
更新日期：2009-02-01 00:00:00
abstract：:In 2002, new developments in anti-angiogenic strategies encompassed two main aspects. Firstly, essential improvements were made in the field of methodology : novel techniques permitted to measure directly on patients the biological effects induced by anti-angiogenic treatments in the course of clinical trials. Secondl...
journal_title：Bulletin du cancer
pub_type： 杂志文章,评审
doi：
更新日期：2003-01-01 00:00:00
abstract：:The diet of the cancer patient is a major focus of prevention and treatment strategy of the recent plans that fight against cancer. It is sometimes reduced to a rapid series of more or less general advice, often interfered by other sources of information, more or less conventional. In this pathological situation where...
journal_title：Bulletin du cancer
pub_type： 杂志文章,评审
doi：10.1684/bdc.2014.1905
更新日期：2014-03-01 00:00:00
abstract：:The great majority of breast cancer cases are not associated with a mutated gene of high penetrance such as BRCA1, BRCA2 and TP53. Genes of low penetrance, frequently mutated in the general population, might play an important role in breast cancer development. The ATM gene, which encodes the ATM protein, mutated in th...
journal_title：Bulletin du cancer
pub_type： 杂志文章,评审
doi：
更新日期：2001-07-01 00:00:00
abstract：:Thymidine kinase (TK) was assayed in the cytosol of 210 primary breast cancers to assess its predictive value for response to first line chemotherapy and hormonotherapy. We performed correlations between TK and the other prognosis factors of breast cancer. TK activity is correlated with SBR grading and estrogen recept...
journal_title：Bulletin du cancer
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:Stereotactic radiation therapy, consisting in irradiating the tumor with a high dose per fraction, has a therapeutic potential because of excellent local control. This technique requires a high accuracy level in order to minimize the risk of normal tissue toxicity. Initially used for cerebral localization, the stereot...
journal_title：Bulletin du cancer
pub_type： 杂志文章,评审
doi：10.1684/bdc.2010.1144
更新日期：2010-07-01 00:00:00
abstract：BACKGROUND:Transmission of oncogenetic information (TOI) by probands to their families is of major importance to organize medical prevention in his family. Little is known about the difficulties that the proband faces when he tries to endorse his "duty to warn". METHODS:To characterize the barriers to TOI, a survey wa...
journal_title：Bulletin du cancer
pub_type： 杂志文章
doi：10.1016/j.bulcan.2014.09.001
更新日期：2015-02-01 00:00:00
abstract：:The radiobiological properties of the hadrons (neutrons, protons, carbon ions) led to their therapeutic use in sarcomas, as a referent therapy or as an alternative to photon therapy. An extensive review of the literature has been conducted to assess the present indications and the perspectives for hadrontherapy. Compa...
journal_title：Bulletin du cancer
pub_type： 杂志文章,评审
doi：10.1684/bdc.2010.1125
更新日期：2010-06-01 00:00:00
abstract：:From a series of 89 clinically inflammatory breast cancers, the authors settle a subgroup of tumors, the infiltrative ductal carcinomas with a pleomorphic structure. They have better histological and hormonal prognostic criteria than the other carcinomas, especially the infiltrative ductal carcinomas with an atypical ...
journal_title：Bulletin du cancer
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:The natural history of breast diseases has changed overtime and successive therapeutic strategies have been adapted accordingly. Recently, biological findings on geno- and phenotypic characteristics of tumor cells offer new basis for the development of treatments that target homogenous and various subtypes of breast c...
journal_title：Bulletin du cancer
pub_type： 杂志文章,评审
doi：10.1684/bdc.2013.1805
更新日期：2013-09-01 00:00:00
abstract：:Melanoma of the female genital tract is a rare location (less than 2% of melanomas all sites combined). These cancers have a very poor prognosis, due to the delay in diagnosis. Vulvar location is about 1% of melanomas then the vaginal location, uterine and ovarian. There is no consensus to date regarding their care, d...
journal_title：Bulletin du cancer
pub_type： 杂志文章,评审
doi：10.1684/bdc.2013.1881
更新日期：2014-01-01 00:00:00
abstract：:Urothelial tumors are very rare in children (to date, only about 150 cases have been reported worlwide). Only 20% occur before the age of ten. The aim of this study is to specify the clinicopathologic features of urothelial tumor in young patients, which require a slightly different approach to treatment. On the basis...
journal_title：Bulletin du cancer
pub_type： 杂志文章,评审
doi：10.1016/j.bulcan.2016.11.018
更新日期：2017-02-01 00:00:00
abstract：:Many complementary and alternatives medicines are offered to patients with cancer. Among them, herbal medicines have a substantial place. These plants are mainly used to reduce adverse effects of anticancer treatments and for specific anticancer properties. Our review shows that only few clinical data support medicina...
journal_title：Bulletin du cancer
pub_type： 杂志文章,评审
doi：10.1684/bdc.2013.1741
更新日期：2013-05-01 00:00:00
abstract：:Bladder tumours are frequent with around 10,000 new cases each year in France. Less than 500 of these cases are not transitional cell carcinoma, the most frequent pathological aspect. The identification of these pathological patterns requires an initial biopsy through transurethral resection. Sarcomatoid, squamous and...
journal_title：Bulletin du cancer
pub_type： 杂志文章
doi：10.1684/bdc.2010.1097
更新日期：2010-01-01 00:00:00
abstract：:Image guided radiation therapy (IGRT) is a major technical innovation of radiotherapy. It allows locating the tumor under the linear accelerator just before the irradiation, by direct visualization (3D mode soft tissue) or indirect visualization (2D mode and radio-opaque markers). The technical implementation of IGRT ...
journal_title：Bulletin du cancer
pub_type： 杂志文章
doi：10.1684/bdc.2010.1140
更新日期：2010-07-01 00:00:00
abstract：:CD44 gene products are potential markers of aggressiveness in different tumor models, a result which prompted us to study clinical neuroblastoma (NB) specimens. CD44 expression was determined by immunostaining of 52 NB with a monoclonal antibody (J173) directed against an epitope common to all CD44 isoforms. All tumor...
journal_title：Bulletin du cancer
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Malnutrition is common in oncology. However, it is often detected too late and nutritional support is sub-optimal. The patient's opinion, although often sought in therapeutic decisions in oncology, does not appear to be frequently taken into account in dietetic management. In NutriCancer2012 study, we interviewed pati...
journal_title：Bulletin du cancer
pub_type： 杂志文章
doi：10.1016/j.bulcan.2017.09.014
更新日期：2017-11-01 00:00:00
abstract：:EXPRESSION III was designed to evaluate the information, needs and expectations of patients with ovarian cancer in different European countries. This abstract focuses on specific results from French OC patients. Two hundred and fifty-seven patients filled a 27-item questionnaire during a medical visit. Median age rang...
journal_title：Bulletin du cancer
pub_type： 杂志文章
doi：10.1016/j.bulcan.2014.09.004
更新日期：2015-03-01 00:00:00
abstract：INTRODUCTION:Breast cancer of young women has medico-psychological issues, particularly in the perspective of "after cancer" period. The objective of the study was to analyze the changes in management, in a decade including the publication of Cancer Plans I and II. METHODS:This retrospective unicentric study involved ...
journal_title：Bulletin du cancer
pub_type： 杂志文章
doi：10.1016/S0007-4551(20)30049-7
更新日期：2019-12-01 00:00:00
abstract：:The evolution of a high grade glioblastoma of a patient undergoing radio-therapy has been analysed by considering a mathematical model which simulates the brain tumour growth within a two-dimensional domain defined by the brain and ventricles geometry. This simulated behaviour was compared with morphological data obta...
journal_title：Bulletin du cancer
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
abstract：:During their life, cells are exposed to a wide variety of extracellular stimuli and have to develop appropriate biological responses. Signal transduction from the plasma membrane, which is in contact with the extracellular environment, to the nucleus, where gene expression is achieved, thus represents a fundamental pr...
journal_title：Bulletin du cancer
pub_type： 杂志文章,评审
doi：
更新日期：1998-06-01 00:00:00
abstract：:Sentinel lymph node (SLN) biopsy is fast becoming the standard for testing lymph node involvement in many institutions. However questions remain as to stage underestimation. The aim of this study was to analyse this specific risk in a retrospective study. Between 1975 to 1999, 1,636 patients underwent a breast cancer ...
journal_title：Bulletin du cancer
pub_type： 临床试验,杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：:Since the results of bone marrow transplantation for acute leukemia in relapse have been poor (less than 20% long term survival), an attempt of bone marrow transplantation for acute leukemia in remission has been performed since 1976 by the Seattle Group. The results are very encouraging. In ANL transplanted in first ...
journal_title：Bulletin du cancer
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:Patients with advanced cancer often experience with physical impairment and loss of autonomy sometimes preceding inexorable death. It is that can emerge Suicidal ideation occasionally associated with suicidal attempt can arise in this particular context but also following the initial diagnostic talk and during all the...
journal_title：Bulletin du cancer
pub_type： 杂志文章
doi：
更新日期：2006-07-01 00:00:00
abstract：:The organization of the management of pain and other symptoms all along the cancer disease, of psychological support and palliative care is a complex question that does not correspond to any perfectly established model, both in France and abroad. Different structures are implied in there care and coexist with an insuf...
journal_title：Bulletin du cancer
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
abstract：:The schooling of young people with cancer poses various problems, similar to those experienced by disabled or chronically ill children. Published data on this subject in the medical literature comes mainly from studies carried out in the USA and the UK. Cancer and its treatment can cause both direct (learning difficul...
journal_title：Bulletin du cancer
pub_type： 杂志文章,评审
doi：
更新日期：2007-04-01 00:00:00
abstract：:Basal-like carcinomas represent 10 to 15% of invasive breast carcinomas and have been identified from gene expression studies. Morphologically, these tumors are undifferentiated histopronostic grade 3 carcinomas, identified in clinical practice according to their immunophenotype "triple zero" (estrogen, progesterone a...
journal_title：Bulletin du cancer
pub_type： 杂志文章
doi：10.1684/bdc.2010.1062
更新日期：2010-03-01 00:00:00
abstract：:Among genito-urinary tumors, gemcitabine is indicated only for the treatment of locally advanced and metastatic urothelial cancer. The combination of gemcitabine and cisplatin is a new standard in this disease. Gemcitabine shows activity in refractory germ cell tumors. In salvage treatment its role is ongoing evaluati...
journal_title：Bulletin du cancer
pub_type： 杂志文章,评审
doi：
更新日期：2007-01-01 00:00:00
