Expression of activated gelatinase in human invasive breast carcinoma.


:The expression of both 92- and 72-kDa gelatinases has been studied in 20 samples of human breast carcinoma by the technique of gelatin zymography. This technique allowed the relative amount of each gelatinase to be determined in small samples of tissue (< 10 mg). More importantly, active and latent forms of the two gelatinases were resolved. Two samples (10-20 mg) were cut from each piece of tumour in order to monitor the variability of gelatinase distribution within that section of tumour. The 72-kDa latent progelatinase was present in 15 of the 20 tumours, with trace amounts in two others. The 62-kDa activated form of this gelatinase was detected in all 15 of the tumours in which the latent form was present. The 92-kDa latent progelatinase was present in 11 of the 20 tumours, with trace amounts in four others. However, the 82-kDa activated form of this gelatinase was only clearly detected in two tumours, although three others showed the presence of trace amounts. The ratio of active to latent forms of the 72-kDa gelatinase ranged from 0.9 to 3.6. There were no marked correlations between gelatinase expression and established staging and prognostic markers. Analysis of three samples of fibroadenoma revealed only very low levels of gelatinase expression. On the basis of these results, activation of the 72-kDa progelatinase appears to be a more common event in invasive breast carcinoma than activation of the 92-kDa progelatinase. However, neither proteinase showed a correlation with metastatic progression, as measured by lymph node involvement.


Clin Exp Metastasis


Brown PD,Bloxidge RE,Anderson E,Howell A




Has Abstract


1993-03-01 00:00:00












  • CD44 expression and hyaluronic acid binding of malignant glioma cells.

    abstract::The mechanisms leading to rapid invasive growth of malignant gliomas are poorly understood. Expression of the hyaluronic acid (HA) receptor CD44 and adhesion to HA are involved in invasive properties. Our previous studies have shown that malignant glioma cells are able to adhere to extracellular HA. Here we investigat...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Knüpfer MM,Poppenborg H,Hotfilder M,Kühnel K,Wolff JE,Domula M

    更新日期:1999-02-01 00:00:00

  • Metastatic potential of human CX-1 colon adenocarcinoma cells is dependent on the expression of sialosyl Le(a) antigen.

    abstract::Several lines of evidence indicate that sialosyl Le(a), tumor-associated carbohydrate antigen present on human colon carcinoma cells, is involved in formation of metastases. To study the role of this carbohydrate structure in development of metastases, we have used the clone of human colon carcinoma CX-1 cells transfe...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Opolski A,Laskowska A,Madej J,Wietrzyk J,Kłopocki A,Radzikowski C,Ugorski M

    更新日期:1998-11-01 00:00:00

  • Multiple effects of transfection with interleukin 2 and/or interferon gamma on the behavior of mouse T lymphoma cells.

    abstract::We have previously reported that transfection of mouse interferon gamma (IFN-gamma) in H-2Kk-positive BW variants (BW-Sp3) abolishes tumorigenicity and reduces metastatic capacity. To further increase the immunogenicity of BW-Sp3 cells, the gene for human interleukin 2 (huIL-2) was transfected in these cancer cells. S...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Geldhof AB,Van Ginderachter J,Vandersteen P,Raes G,De Baetselier P

    更新日期:1998-07-01 00:00:00

  • Angiogenesis and the role of epigenetics in metastasis.

    abstract::The major obstacle to devising effective ways to treat cancer is its heterogeneity and genetic instability. It was originally postulated that targeting the process of tumor angiogenesis could circumvent this problem, as it involves genetically stable epigenetically controlled host stroma. Thus, anti-angiogenic approac...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Coomber BL,Yu JL,Fathers KE,Plumb C,Rak JW

    更新日期:2003-01-01 00:00:00

  • SCUBE3 regulation of early lung cancer angiogenesis and metastatic progression.

    abstract::Signal peptide-CUB-EGF-like domain-containing protein 3 (SCUBE3) is strongly expressed in extremely invasive lung carcinoma. We showed in our previous study that SCUBE3 triggers the transforming growth factor-β pathway and subsequently promotes tumor angiogenesis and the epithelial-mesenchymal transition (EMT). Howeve...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Chou CH,Cheng YF,Siow TY,Kumar A,Peck K,Chang C

    更新日期:2013-08-01 00:00:00

  • Repetitive and site-specific molecular staging of prostate cancer using nested reverse transcriptase polymerase chain reaction for prostate specific antigen and prostate specific membrane antigen.

    abstract::We performed repetitive molecular staging using, nested rt-PCR for PSA and PSM at the peripheral blood (PB) and bone marrow (BM) of patients with prostate cancer (Pr.Ca) and benign prostate hyperplasia (BPH) after transrectal ultrasonography-guided biopsy (TRUS-B; 6-9 biopsies/patient), Pr.Ca patients after radical pr...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Koutsilieris M,Lembessis P,Luu-The V,Sourla A

    更新日期:1999-01-01 00:00:00

  • Bosentan inhibits tumor vascularization and bone metastasis in an immunocompetent skin-fold chamber model of breast carcinoma cell metastasis.

    abstract::Angiogenic factors including endothelin-1 (ET-1) play a key role in the progression of breast metastases to bone. We investigated the impact of ET-1 on the development of bone metastases in an immunocompetent murine skin-fold chamber model. Murine mammary carcinoma 4T1 was injected in a skin-fold chamber implanted on ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Dréau D,Karaa A,Culberson C,Wyan H,McKillop IH,Clemens MG

    更新日期:2006-01-01 00:00:00

  • The efficacy of Tilmanocept in sentinel lymph mode mapping and identification in breast cancer patients: a comparative review and meta-analysis of the ⁹⁹mTc-labeled nanocolloid human serum albumin standard of care.

    abstract::Sentinel lymph node (SLN) mapping is common, however question remains as to what the ideal imaging agent is and how such an agent might provide reliable and stable localization of SLNs. (99m)Tc-labeled nanocolloid human serum albumin (Nanocoll) is the most commonly used radio-labeled colloid in Europe and remains the ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,meta分析,评审


    authors: Tokin CA,Cope FO,Metz WL,Blue MS,Potter BM,Abbruzzese BC,Hartman RD,Joy MT,King DW,Christman LA,Vera DR,Wallace AM

    更新日期:2012-10-01 00:00:00

  • Astrocyte-induced Reelin expression drives proliferation of Her2+ breast cancer metastases.

    abstract::Breast cancer metastasis to the brain develops after a clinical latency of years to even decades, suggesting that colonization of the brain is the most challenging step of the metastatic cascade. However, the underlying mechanisms used by breast cancer cells to successfully colonize the brain's microenvironment remain...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Jandial R,Choy C,Levy DM,Chen MY,Ansari KI

    更新日期:2017-02-01 00:00:00

  • An examination of the effects of hypoxia, acidosis, and glucose starvation on the expression of metastasis-associated genes in murine tumor cells.

    abstract::Tumor cells exposed to a growth stress such as low pH, glucose starvation and hypoxia have been shown to exhibit a transient increase in experimental metastatic potential, particularly when allowed to recover under normal growth conditions for a period of 24-48 h. In this study we examined whether this increase in met...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Jang A,Hill RP

    更新日期:1997-09-01 00:00:00

  • S100A11 is a potential prognostic marker for clear cell renal cell carcinoma.

    abstract::Clear cell renal cell carcinoma (ccRCC) is one of few cancers with rising incidence in North America. The prognosis of ccRCC is variable and difficult to predict. Stratification of patients according to disease aggressiveness can significantly improve patient management. We investigated the expression of the S100A11 p...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Gabril M,Girgis H,Scorilas A,Rotondo F,Wala S,Bjarnason GA,Ding Q,Evans A,Tawedrous E,Pasic M,Finelli A,Al-Haddad S,Yousef GM

    更新日期:2016-01-01 00:00:00

  • Metrazol enhances brain penetration and therapeutic efficacy of some anticancer agents: implications for brain metastases.

    abstract::The concurrent administration of Metrazol (60 mg/kg, i.v.) to anaesthetized rats enhances the cerebral penetration of the anticancer agent razoxane. Such an enhancement leads to an increase in the therapeutic efficacy of razoxane against intracerebrally sequestered L1210 leukaemia cells in mice. The combination of Met...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Greig N,Newell D,Hellmann K

    更新日期:1984-01-01 00:00:00

  • Predictors of prognosis of synchronous brain metastases in small-cell lung cancer patients.

    abstract::Patients with small cell lung cancer (SCLC) are more likely to have synchronous brain metastasis (SBM) at the time of diagnosis than patients with any other extracranial primary malignancy. We sought to identify which factors predicted an increased risk of SBM in SCLC as well as which factors affected the prognosis of...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Reddy SP,Dowell JE,Pan E

    更新日期:2020-08-01 00:00:00

  • Ras suppressor-1 promotes apoptosis in breast cancer cells by inhibiting PINCH-1 and activating p53-upregulated-modulator of apoptosis (PUMA); verification from metastatic breast cancer human samples.

    abstract::Metastasis, responsible for most deaths from breast cancer (BC), is a multistep process leading to cancer cell spread. Extracellular matrix (ECM)-related adhesion and apoptosis resistance play pivotal role in metastasis. Ras suppressor-1 (RSU-1) localizes to cell-ECM adhesions and binds to pro-survival adhesion protei...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Giotopoulou N,Valiakou V,Papanikolaou V,Dubos S,Athanassiou E,Tsezou A,Zacharia LC,Gkretsi V

    更新日期:2015-03-01 00:00:00

  • Transcriptional regulation of osteopontin and the metastatic phenotype: evidence for a Ras-activated enhancer in the human OPN promoter.

    abstract::Elevated osteopontin (OPN) transcription often correlates with increased metastatic potential of transformed cells, and in several model systems OPN--whether produced by the tumor cells or by stromal cells - has been shown to enhance metastatic ability. Sequence elements in the OPN promoter have been identified on the...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Denhardt DT,Mistretta D,Chambers AF,Krishna S,Porter JF,Raghuram S,Rittling SR

    更新日期:2003-01-01 00:00:00

  • Editorial: Therapy-induced metastasis.

    abstract::The idea for this Special Issue originated from our recent review in Nature Reviews Clinical Oncology entitled "Does the mobilization of circulating tumour cells during cancer therapy cause metastasis?" Martin et al. (Nat Rev Clin Oncol 14:32-44, 2017). While preparing this review, it became evident that an overwhelmi...

    journal_title:Clinical & experimental metastasis

    pub_type: 社论


    authors: Martin OA,Anderson RL

    更新日期:2018-04-01 00:00:00

  • Skeletal impact of 17β-estradiol in T cell-deficient mice: age-dependent bone effects and osteosarcoma formation.

    abstract::Estrogen (E2)-dependent ER+ breast cancer, the most common breast cancer subtype, is also the most likely to metastasize to bone and form osteolytic lesions. However, ER+ breast cancer bone metastasis human xenograft models in nude mice are rarely studied due to complexities associated with distinguishing possible tum...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Cheng JN,Frye JB,Whitman SA,Funk JL

    更新日期:2020-04-01 00:00:00

  • Histopathologic analysis of bone marrow and bone metastasis in murine neuroblastoma.

    abstract::To elucidate the development of bone metastasis in human neuroblastoma, bone marrow and bone metastases were analysed histologically in a hematogeneous metastasis model of murine neuroblastoma. The bone marrow metastasis occurred initially in the bone marrow sinusoid where tumor cells adhered and extravasated to bone ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Yoshino K,Tanabe M,Ohnuma N,Takahashi H

    更新日期:1996-10-01 00:00:00

  • Establishment of highly metastatic tumor clones derived from a skin squamous cell carcinoma (SqC-NH), D-1 and F-3, with distinct features of pulmonary metastases.

    abstract::We have established two highly metastatic tumor clones, D-1 and F-3, which have the distinct features of undergoing pulmonary metastases. Both clones were derived from a skin squamous cell carcinoma (SqC-NH), which had spontaneously occurred in DS-Nh mice. F-3 was morphologically spindle-shaped in tumor mass and adher...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Maekawa R,Kishimoto Y,Sato K,Homma M

    更新日期:1993-07-01 00:00:00

  • Lymphatic metastasis in breast cancer: importance and new insights into cellular and molecular mechanisms.

    abstract::Lymph node metastasis is the main prognosis factor in a number of malignancies, including breast carcinomas. The means by which lymph node metastases arise is not fully understood, and many questions remain about their importance in the further spread of breast cancer. Nevertheless, a number of key cellular and molecu...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Eccles S,Paon L,Sleeman J

    更新日期:2007-01-01 00:00:00

  • The protein kinase C inhibitor, H7, inhibits tumor cell invasion and metastasis in mouse melanoma via suppression of ERK1/2.

    abstract::Protein kinase C (PKC) has been shown to be a signal transducer during tumorigenesis, tumor cell invasion, and metastasis. Recent studies have reported that the PKC inhibitor, 7-hydroxystaurosporine, inhibits tumor cell invasion. However, the molecular mechanisms of this inhibition of invasion and metastasis are not w...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Tsubaki M,Matsuoka H,Yamamoto C,Kato C,Ogaki M,Satou T,Itoh T,Kusunoki T,Tanimori Y,Nishida S

    更新日期:2007-01-01 00:00:00

  • Relation of matrilysin messenger RNA expression with invasive activity in human gastric cancer.

    abstract::Matrilysin is a member of the matrix metalloproteinase gene family which is believed to play an important role in tumor progression. Expression of matrilysin mRNA was examined by reverse transcription-polymerase chain reaction combined with Southern blot analysis in 46 human primary gastric cancers. Overexpression of ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Senota A,Itoh F,Yamamoto H,Adachi Y,Hinoda Y,Imai K

    更新日期:1998-05-01 00:00:00

  • Alterations in Cx43 and OB-cadherin affect breast cancer cell metastatic potential.

    abstract::Emerging evidence suggests that gap junctional intercellular communication (GJIC) and expression of connexins (Cx) contribute to the metastatic potential of breast cancer cells. To more directly address this, an aggressive bone metastasis breast cancer cell line, MDA-MET (MET), was stably transfected with human Cx43 c...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Li Z,Zhou Z,Donahue HJ

    更新日期:2008-01-01 00:00:00

  • Circulating microRNAs in head and neck cancer: a scoping review of methods.

    abstract::Circulating microRNAs have been described as head and neck cancer biomarkers in multiple anatomical subsites including the oral cavity, nasopharynx, larynx, salivary glands and the skin. While there is an expanding volume of published literature showing the significance of individual or panels of microRNAs, the clinic...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Dharmawardana N,Ooi EH,Woods C,Hussey D

    更新日期:2019-06-01 00:00:00

  • Leptin utilizes Jun N-terminal kinases to stimulate the invasion of MCF-7 breast cancer cells.

    abstract::In breast tumors, high levels of leptin have been associated with increased incidence of breast cancer metastasis. Breast cancer metastasis is directly associated with breast cancer cell invasion. However, whether leptin could augment breast cancer cell invasion is not known. Here we showed that leptin increased the i...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: McMurtry V,Simeone AM,Nieves-Alicea R,Tari AM

    更新日期:2009-01-01 00:00:00

  • SURVIVIN as a marker for quiescent-breast cancer stem cells-An intermediate, adherent, pre-requisite phase of breast cancer metastasis.

    abstract::Cancer stem cells drive the metastatic cascade by undergoing epithelial to mesenchymal transition (EMT) and again mesenchymal to epithelial transition (MET). Using multiple breast cancer cell lines including cigarette smoke induced breast cancer cells and tumor derived primary cells from patient sample; we developed a...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Siddharth S,Das S,Nayak A,Kundu CN

    更新日期:2016-10-01 00:00:00

  • Is adjuvant treatment with vinblastine effective in reducing the occurrence of distant metastasis in limited squamous cell lung cancer? A randomized study.

    abstract::In order to study the usefulness of treatment with vinblastine (VLB) in the prevention of cancer metastasis in squamous cell lung cancer, 50 patients with locoregional disease were randomized to receive either locoregional RT alone (group A) or a weekly intravenous bolus injection of VLB (6 mg/m2) concurrently with an...

    journal_title:Clinical & experimental metastasis

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Schallier DC,De Neve WJ,De Greve JL,Van Belle SP,De Wasch GJ,Dotremont G,Storme GA

    更新日期:1988-01-01 00:00:00

  • Comparative studies between nude and scid mice on the growth and metastatic behavior of xenografted human tumors.

    abstract::The growth and metastatic behavior of three human tumor cell lines and a human colon carcinoma previously passaged in vivo were compared between nude mice and scid mice after xenotransplantation. The three human tumor lines included a bladder carcinoma (T24B), a melanoma (RPMI 7931) and a lacZ gene-transduced breast c...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Xie X,Brünner N,Jensen G,Albrectsen J,Gotthardsen B,Rygaard J

    更新日期:1992-05-01 00:00:00

  • The clinicopathological evaluation of the breast cancer patients with brain metastases: predictors of survival.

    abstract::We aimed to define the clinicopathologic characteristics of breast cancer (BC) patients with brain metastasis (BM) and to investigate the effect of these parameters on survival. Seventy-nine patients diagnosed with BC and symptomatic BM between 1995 and 2011 were retrospectively evaluated. The relationship between cli...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Tarhan MO,Demir L,Somali I,Yigit S,Erten C,Alacacioglu A,Ellidokuz H,Seseogullari O,Kucukzeybek Y,Can A,Dirican A,Bayoglu V,Akyol M

    更新日期:2013-02-01 00:00:00

  • Growth and metastasis of hypermotile, hyperinvasive cancer cells selected in vitro by rapid locomotion under various conditions.

    abstract::Cancer cells selected from a cultured murine fibrosarcoma by rapid migration through micropore membranes moved considerably faster through such membranes and invaded biological tissues much more efficiently than did the unselected parent cells. The present data show that populations of cells selected by unstimulated m...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Grimstad IA

    更新日期:1988-07-01 00:00:00