Adenosine alters glucose use during ischemia and reperfusion in isolated rat hearts.


BACKGROUND:Adenosine possesses marked cardioprotective properties, but the mechanisms for this beneficial effect are unclear. The objective of this study was to determine the effect of adenosine given before ischemia or at reperfusion on mechanical function, glucose oxidation, glycolysis, and metabolite levels in isolated, paced (280 beats per minute) working rat hearts. METHODS AND RESULTS:Hearts were perfused with Krebs-Henseleit buffer containing 11 mM glucose, 1.2 mM palmitate, and 500 microU.mL-1 insulin at an 11.5 mm Hg left atrial preload and 80 mm Hg aortic afterload. Adenosine (100 microM) pretreatment or adenosine (100 microM) at reperfusion markedly increased the recovery of mechanical function (from 44% to 81% and 96%, respectively) after 60 minutes of low-flow ischemia (coronary flow, 0.5 mL.min-1). Glucose oxidation (mumol.min-1 x g dry wt-1) was inhibited during ischemia (from 0.44 +/- 0.04 to 0.12 +/- 0.01), and this was not altered by adenosine (100 microM). During reperfusion, glucose oxidation recovered (to 0.38 +/- 0.02) and adenosine (100 microM), given at reperfusion, further increased glucose oxidation (to 0.52 +/- 0.06). The rate of glycolysis (mumol.min-1 x g dry wt-1), which was unaffected by ischemia per se, was inhibited by adenosine pretreatment (from 4.7 +/- 0.3 to 2.6 +/- 0.3). During reperfusion, glycolysis was also inhibited by adenosine relative to control (3.9 +/- 0.8) either when present during ischemia (2.6 +/- 0.6) or during reperfusion (1.4 +/- 0.4). These effects of adenosine on glucose metabolism reduced the calculated rate of H+ production attributable to glucose metabolism during the ischemic and reperfusion periods. Tissue lactate levels (mumol.g dry wt-1), which increased during ischemia (from 9.3 +/- 1.1 to 87.4 +/- 10.3) and then declined during reperfusion (to 26.2 +/- 3.7), were depressed further by adenosine pretreatment (to 19.7 +/- 4.1) and by adenosine at reperfusion (to 13.6 +/- 2.1). ATP levels (mumol.g dry wt-1), which were depressed by ischemia (from 18.1 +/- 1.1 to 10.6 +/- 1.3) and tended to be further depressed during reperfusion (to 7.1 +/- 0.7), were increased by adenosine pretreatment (to 14.1 +/- 1.2) and by adenosine at reperfusion (to 15.6 +/- 2.4). CONCLUSIONS:The effects of adenosine on glucose metabolism that would tend to decrease cellular acidosis and hence, Ca2+ overload, may explain the beneficial effects of adenosine on mechanical function observed in these hearts during reperfusion after ischemia.






Finegan BA,Lopaschuk GD,Coulson CS,Clanachan AS




Has Abstract


1993-03-01 00:00:00












  • Reduction in cardiovascular events during pravastatin therapy. Pooled analysis of clinical events of the Pravastatin Atherosclerosis Intervention Program.

    abstract:BACKGROUND:It has been documented that the HMG coenzyme A reductase inhibitors, or statins, can decrease cardiovascular events and mortality in patients with clinical coronary disease and moderately to severely elevated lipid levels. Additional data are required to demonstrate a reduction of vascular events in coronary...


    pub_type: 杂志文章,meta分析


    authors: Byington RP,Jukema JW,Salonen JT,Pitt B,Bruschke AV,Hoen H,Furberg CD,Mancini GB

    更新日期:1995-11-01 00:00:00

  • Ventricular aneurysms complicating coxsackievirus group B, types 1 and 4 murine myocarditis.

    abstract::Suckling Swiss Webster mice were inoculated with 10(4)TCD50 of coxsackieviruses, group B types 1 or 4. Virulent necrotizing myocarditis resulted in 185 infected mice. Of the latter group, three (14.3%) nurslings on the 17th and 23rd day after inoculations had left ventricular aneurysms postmortem. None of 61 concurren...


    pub_type: 杂志文章


    authors: El-Khatib MR,Chason JL,Lerner AM

    更新日期:1979-02-01 00:00:00

  • Influence of the pericardium and ventricular interdependence on left ventricular diastolic and systolic function in patients with heart failure.

    abstract::The pericardium, because of the nature of its pressure-volume relation, can influence left ventricular diastolic and systolic pump function. During an acute increase in heart size that results in the stretching of the pericardium, the elastic properties of the pericardium restrict further cardiac dilatation and curtai...


    pub_type: 杂志文章


    authors: Janicki JS

    更新日期:1990-02-01 00:00:00

  • Survival and function of bioengineered cardiac grafts.

    abstract:INTRODUCTION:Patients with congenital heart disease frequently require graft material for repair of cardiac defects. However, currently available grafts lack growth potential and are noncontractile and thrombogenic. We have developed a viable cardiac graft that contracts spontaneously in tissue culture by seeding cells...


    pub_type: 杂志文章


    authors: Li RK,Jia ZQ,Weisel RD,Mickle DA,Choi A,Yau TM

    更新日期:1999-11-09 00:00:00

  • Placental Growth Factor as an Indicator of Maternal Cardiovascular Risk After Pregnancy.

    abstract:BACKGROUND:Angiogenic placental growth factor (PlGF) concentrations rise during pregnancy, peaking at the end of midpregnancy. Low PlGF concentrations during pregnancy are associated with pregnancy complications with recognized later-life cardiovascular risk. We hypothesized that low PlGF concentrations, especially in ...


    pub_type: 杂志文章


    authors: Benschop L,Schalekamp-Timmermans S,Broere-Brown ZA,Roeters van Lennep JE,Jaddoe VWV,Roos-Hesselink JW,Ikram MK,Steegers EAP,Roberts JM,Gandley RE

    更新日期:2019-04-02 00:00:00

  • Concordance for coronary risk factors among spouses.

    abstract::Values for several coronary risk factors, including systolic and diastolic blood pressure, serum cholesterol, triglycerides, blood glucose, uric acid, hemoglobin, weight, vitral capacity and cigarette smoking have been found to be similar among spouses in the Framingham Study. However, longitudinal analyses show that...


    pub_type: 杂志文章


    authors: Sackett DL,Anderson GD,Milner R,Feinleib M,Kannel WB

    更新日期:1975-10-01 00:00:00

  • Site-dependent intra-atrial conduction delay. Relationship to initiation of atrial fibrillation.

    abstract:BACKGROUND:Atrial dysrhythmia patients have exaggerated intra-atrial conduction delays and prolonged relative refractoriness in response to atrial premature depolarizations (APDs). Furthermore, atrial fibrillation (AF) is more readily inducible by APDs from the high right atrium (HRA) than the coronary sinus (CS). In t...


    pub_type: 杂志文章


    authors: Papageorgiou P,Monahan K,Boyle NG,Seifert MJ,Beswick P,Zebede J,Epstein LM,Josephson ME

    更新日期:1996-08-01 00:00:00

  • Mechanism of ST elevation and ventricular arrhythmias in an experimental Brugada syndrome model.

    abstract:BACKGROUND:Although phase 2 reentry is said to be responsible for initiation of ventricular tachycardia (VT) in Brugada syndrome, information about the activation sequence during VT is limited. METHODS AND RESULTS:We developed an experimental Brugada syndrome model using a canine isolated right ventricular preparation...


    pub_type: 杂志文章


    authors: Kimura M,Kobayashi T,Owada S,Ashikaga K,Higuma T,Sasaki S,Iwasa A,Motomura S,Okumura K

    更新日期:2004-01-06 00:00:00

  • Serum antibodies against Chlamydia pneumoniae outer membrane protein cross-react with the heavy chain of immunoglobulin in the wall of abdominal aortic aneurysms.

    abstract:BACKGROUND:Chlamydia pneumoniae (Cp) has been demonstrated in arteries and abdominal aortic aneurysms (AAAs). However, the validity of the methods used is questioned, and antibiotic treatment trials have thus far shown disappointing results. Nevertheless, antibodies against the Cp outer membrane proteins (OMPs) have be...


    pub_type: 杂志文章


    authors: Lindholt JS,Støvring J,Østergaard L,Urbonavicius S,Henneberg EW,Honoré B,Vorum H

    更新日期:2004-05-04 00:00:00

  • Randomized COMparison of platelet inhibition with abciximab, tiRofiban and eptifibatide during percutaneous coronary intervention in acute coronary syndromes: the COMPARE trial. Comparison Of Measurements of Platelet aggregation with Aggrastat, Reopro, an

    abstract:BACKGROUND:The relative anti-aggregatory effects of currently prescribed platelet glycoprotein IIb/IIIa receptor antagonists during and after percutaneous coronary intervention for acute coronary syndromes have not been established. METHODS AND RESULTS:We randomized 70 acute coronary syndrome patients undergoing percu...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Batchelor WB,Tolleson TR,Huang Y,Larsen RL,Mantell RM,Dillard P,Davidian M,Zhang D,Cantor WJ,Sketch MH Jr,Ohman EM,Zidar JP,Gretler D,DiBattiste PM,Tcheng JE,Califf RM,Harrington RA

    更新日期:2002-09-17 00:00:00

  • Presence of tissue factor pathway inhibitor in human atherosclerotic plaques is associated with reduced tissue factor activity.

    abstract:BACKGROUND:Plaque disruption and exposure of subendothelial procoagulants such as tissue factor (TF) to circulating factor VII/VIIa (FVII/VIIa) lead to intravascular thrombosis. Tissue factor pathway inhibitor (TFPI) is an endogenous inhibitor of TF-induced coagulation that binds to factor Xa and the TF-FVIIa catalytic...


    pub_type: 杂志文章


    authors: Caplice NM,Mueske CS,Kleppe LS,Simari RD

    更新日期:1998-09-15 00:00:00

  • Attenuation of exercise-induced myocardial ischemia in dogs with recruitment of coronary vasodilator reserve by nifedipine.

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    pub_type: 杂志文章


    authors: Heusch G,Guth BD,Seitelberger R,Ross J Jr

    更新日期:1987-02-01 00:00:00

  • Why is off-pump coronary surgery uncommon in Canada? Results of a population-based survey of Canadian heart surgeons.

    abstract:BACKGROUND:Off-pump coronary artery bypass (OPCAB) is proposed to improve clinical outcomes and decrease resource use. However, off-pump surgery is not widely used in Canada. The purpose of this study was to determine the current use of OPCAB in Canada and determine why surgeons have not adopted this technique. METHOD...


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    更新日期:2004-09-14 00:00:00

  • B-type natriuretic peptide and ischemia in patients with stable coronary disease: data from the Heart and Soul study.

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    pub_type: 杂志文章


    authors: Bibbins-Domingo K,Ansari M,Schiller NB,Massie B,Whooley MA

    更新日期:2003-12-16 00:00:00

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    pub_type: 杂志文章


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    更新日期:2006-07-04 00:00:00

  • Visceral adiposity and the prevalence of hypertension in Japanese Americans.

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    pub_type: 杂志文章


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    更新日期:2003-10-07 00:00:00

  • Longitudinal myocardial function assessed by tissue velocity, strain, and strain rate tissue Doppler echocardiography in patients with AL (primary) cardiac amyloidosis.

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    pub_type: 临床试验,杂志文章


    authors: Koyama J,Ray-Sequin PA,Falk RH

    更新日期:2003-05-20 00:00:00

  • Correlation of the width of the QRS complex with the pathologic anatomy of the cardiac conduction system in patients with chronic complete atrioventricular block.

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    pub_type: 杂志文章


    authors: Ohkawa S,Hackel DB,Ideker RE

    更新日期:1981-04-01 00:00:00

  • Ion channel remodeling is related to intraoperative atrial effective refractory periods in patients with paroxysmal and persistent atrial fibrillation.

    abstract:BACKGROUND:Sustained shortening of the atrial effective refractory period (AERP), probably due to reduction in the L-type calcium current, is a major factor in the initiation and maintenance of atrial fibrillation (AF). We investigated underlying molecular changes by studying the relation between gene expression of the...


    pub_type: 杂志文章


    authors: Brundel BJ,Van Gelder IC,Henning RH,Tieleman RG,Tuinenburg AE,Wietses M,Grandjean JG,Van Gilst WH,Crijns HJ

    更新日期:2001-02-06 00:00:00

  • Hypoxia/reoxygenation stimulates intracellular calcium oscillations in human aortic endothelial cells.

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    pub_type: 杂志文章


    authors: Hu Q,Ziegelstein RC

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  • Hospital delays in reperfusion for ST-elevation myocardial infarction: implications when selecting a reperfusion strategy.

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    pub_type: 杂志文章


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  • Thrombolytic and pharmacokinetic properties of chimeric tissue-type and urokinase-type plasminogen activators.

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    pub_type: 杂志文章


    authors: Collen D,Lu HR,Lijnen HR,Nelles L,Stassen JM

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  • Eliminating bradyarrhythmias after orthotopic heart transplantation.

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    pub_type: 杂志文章


    authors: Rothman SA,Jeevanandam V,Combs WG,Furukawa S,Hsia HH,Eisen HJ,Buxton AE,Miller JM

    更新日期:1996-11-01 00:00:00

  • Cardiac allograft vasculopathy: a review.

    abstract::Cardiac allograft vasculopathy (CAV) remains a troublesome long-term complication of heart transplantation. It is manifested by a unique and unusually accelerated form of coronary disease affecting both intramural and epicardial coronary arteries and veins.CAV is characterized by vascular injury induced by a variety o...


    pub_type: 杂志文章,评审


    authors: Weis M,von Scheidt W

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    pub_type: 杂志文章


    authors: Moss J,Fahmy NR,Sunder N,Beaven MA

    更新日期:1981-01-01 00:00:00

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    authors: Moliterno DJ,Lange RA,Meidell RS,Willard JE,Leffert CC,Gerard RD,Boerwinkle E,Hobbs HH,Hillis LD

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    pub_type: 杂志文章


    authors: Nicolini FA,Mehta JL,Nichols WW,Saldeen TG,Grant M

    更新日期:1990-03-01 00:00:00

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    pub_type: 杂志文章


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    更新日期:1997-10-21 00:00:00

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    pub_type: 杂志文章


    authors: St John Sutton MG,Raichlen JS,Reichek N,Huff DS

    更新日期:1984-12-01 00:00:00