Abstract:
:Fluorescent retroviral envelope (Env) proteins were developed for direct visualization of viral particles. By fusing the enhanced green fluorescent protein (eGFP) to the N terminus of the amphotropic 4070A envelope protein, extracellular presentation of eGFP was achieved. Viruses incorporated the modified Env protein and efficiently infected cells. We used the GFP-tagged viruses for staining retrovirus receptor-positive cells, thereby circumventing indirect labeling techniques. By generating cells which conditionally expressed the GFP-tagged Env protein, we could confirm an inverse correlation between retroviral Env expression and infectivity (superinfection). eGFP-tagged virus particles are suitable for monitoring the dynamics of virus-cell interactions.
journal_name
J Viroljournal_title
Journal of virologyauthors
Spitzer D,Dittmar KE,Rohde M,Hauser H,Wirth Ddoi
10.1128/jvi.77.10.6070-6075.2003subject
Has Abstractpub_date
2003-05-01 00:00:00pages
6070-5issue
10eissn
0022-538Xissn
1098-5514journal_volume
77pub_type
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