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Electrotransfer at MR imaging: tool for optimization of gene transfer protocols--feasibility study in mice.

Abstract：

PURPOSE:To test, by using an electrotransfer protocol for the transfection of skeletal muscle with naked plasmid complementary DNA, whether in vivo magnetic resonance (MR) imaging can help delineate either the spatial extent of the electric field when contrast agent is injected intraperitoneally or the transfection area when contrast agent is injected locally. MATERIALS AND METHODS:Three groups of five mice each were examined at 4 T. Gadopentetate dimeglumine was injected intraperitoneally before electroporation in group 1 and after electroporation in group 2. In group 3, gadopentetate dimeglumine was coinjected in situ with plasmid pCMV-beta Gal in the gastrocnemius muscle before electroporation. MR imaging and muscle preparation for histologic examination were performed 3 days later. On T1-weighted images, increase of muscle signal intensity was determined in regions of interest (ROIs) of treated legs and compared with contralateral ROIs. Comparison of signal intensity increase between groups 1 and 2 was performed with Kruskal-Wallis test. RESULTS:In groups 1 and 3, T1-weighted images of treated muscle showed zones of strongly increased signal intensity. In corresponding ROIs of groups 1, 2, and 3, the mean T1-weighted signal intensity increase at day 3 was 1.64 +/- 0.20 (SD), 1.16 +/- 0.06, and 1.58 +/- 0.17, respectively. The difference between groups 1 and 2 (ie, gadopentetate dimeglumine injected before and after electrotransfer) was significant (P <.001) both without and with correction for T2 variation (1.47 +/- 0.19 and 1.04 +/- 0.09, respectively). In group 3, after in situ coinjection of gadopentetate dimeglumine and plasmid, the area of increased signal intensity revealed at ex vivo MR imaging of the muscle showed a reasonable concordance with the transfected area revealed with beta-galactosidase on histologic sections. CONCLUSION:In vivo and ex vivo results indicate that atraumatic visualization of the permeabilized and transfected area is possible.

journal_name

Radiology

journal_title

Radiology

authors

Paturneau-Jouas M,Parzy E,Vidal G,Carlier PG,Wary C,Vilquin JT,de Kerviler E,Schwartz K,Leroy-Willig A

doi

10.1148/radiol.2283020482

subject

Has Abstract

pub_date

2003-09-01 00:00:00

pages

768-75

issue

eissn

0033-8419

issn

1527-1315

pii

2283020482

journal_volume

228

pub_type

杂志文章

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Electrotransfer at MR imaging: tool for optimization of gene transfer protocols--feasibility study in mice.