Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens.

Abstract:

:The present report describes an alternative method for in vitro detection of HIV-1-specific antibody secretion in 24h of culture employing as stimulant of peripheral blood mononuclear cells the disrupted inactivated whole virus adsorbed onto microwells in a commercial ELISA kit plates. The results obtained from this technique have showed high sensitivity and specificity since it was capable of detecting HIV-1 infection early after birth. There were neither false-positivity nor false-negativity when blood samples obtained from HIV-1 seronegative asymptomatic individuals, and HIV-1 seropositive adult patients were analyzed. This rapid, low cost, simple, highly sensitive and specific assay can be extremely useful for early diagnosis of pediatric HIV infection.

journal_name

Mem Inst Oswaldo Cruz

authors

Caterino-de-Araujo A

doi

10.1590/s0074-02761992000200012

subject

Has Abstract

pub_date

1992-04-01 00:00:00

pages

239-47

issue

2

eissn

0074-0276

issn

1678-8060

pii

S0074-02761992000200012

journal_volume

87

pub_type

杂志文章
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    pub_type: 杂志文章,评审

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    pub_type: 杂志文章

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    更新日期:1987-01-01 00:00:00

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    pub_type: 杂志文章

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    更新日期:2007-09-01 00:00:00

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