Abstract：
:Several studies on biotin intestinal transport in the hamster have shown a biotin-specific carrier, but there are conflicting reports on whether it is transported actively, or by facilitated diffusion and on its Na+ dependence. We have studied it for the first time using brush-border membrane vesicles (BBMV), with concentrations in a more physiological (nanomolar) range and found an overshoot component, evidencing a carrier-mediated active process, driving the vitamin against a concentration gradient. Uptake was substantially reduced when potassium substituted for sodium. When the vesicles were treated with trypsin, Na(+)-dependent uptake was markedly reduced and the overshoot phenomenon was abolished, providing additional evidence for the carrier-mediated transport. The amount of uptake in a K+ gradient was considered due to passive diffusion and was about 30% of that observed in a Na+ gradient. A similar amount was observed when trypsinized vesicles were incubated in this latter gradient. Our results indicate that in the hamster's brush border intestinal epithelium, Na(+)-dependent active transport is the most important component in the intestinal uptake of biotin at nanomolar concentrations.
journal_name
Arch Med Resjournal_title
Archives of medical researchauthors
León-Del-Río A,Hol-Soto-Borja D,Velázquez Asubject
Has Abstractpub_date
1993-07-01 00:00:00pages
143-6issue
2eissn
0188-4409issn
1873-5487journal_volume
24pub_type
杂志文章abstract：BACKGROUND:A comprehensive understanding of the immune response induced by Mycobacterium bovis Bacille Calmette-Guérin in activation of protective T cells against tuberculosis is important to develop effective therapies to combat this disease. In this study, our experiments were designed to determine effects of transfo...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/s0188-4409(02)00463-0
更新日期：2003-01-01 00:00:00
abstract：:To know the activity of antimeningococcal immunoglobulin, Balb/c mice of 18-22 g of body weight were challenged with 5 serotype B strains of Neisseria meningitidis (Nm) isolated from patients of different Latin American countries. The specific antimeningococcal Ig was extracted from the serum of volunteers previously ...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND AND AIMS:Streptococcus pneumoniae is the leading cause of acute otitis media, pneumonia, meningitis, and sepsis. The heptavalent pneumococcal conjugate vaccine (PCV7) was incorporated into the national immunization program in Mexico in 2008. The aim of the study was to analyze the frequency of S. pneumoniae ...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2015.02.004
更新日期：2015-02-01 00:00:00
abstract：:The effect of electric field stimulation for promoting axonal growth between sural to facial nerve grafts in cases of congenital permanent facial palsy associated with hemifacial microsomia was studied. For this purpose, a total of 17 patients with unilateral congenital permanent facial palsy associated with hemifacia...
journal_title：Archives of medical research
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1996-04-01 00:00:00
abstract：BACKGROUND:Cellular events in cisplatin-mediated nephrotoxicity include apoptosis induction, decreased protein synthesis, changes in the subcellular redistribution of Bax mitochondrial dysfunction, DNA injury, increased lipid peroxidation, depletion of glutathione and decrease in enzymatic activity of renal antioxidant...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2006.02.008
更新日期：2006-08-01 00:00:00
abstract：:The fundamental principle of stem cell biology is that cells with the potential for both self-renewal and terminal differentiation into one or more cell types may be found in a given tissue. The corollary of this principle is that the stem cells give rise to tissues in which they reside, the so-called expected tissues...
journal_title：Archives of medical research
pub_type： 杂志文章,评审
doi：10.1016/j.arcmed.2003.09.006
更新日期：2003-11-01 00:00:00
abstract：AIM OF THE STUDY:Rutin and Silibinin are active flavonoid compounds, well-known for possessing multiple biological activities. We have studied how Rutin and Silibinin in combination modulate wide range intracellular signaling cascades as evidenced by in-vitro research. Data obtained from preclinical studies provide evi...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2018.09.008
更新日期：2018-05-01 00:00:00
abstract：BACKGROUND:Afternoon nap sleep was studied in 32 young male medical students who take customary naps to replace loss in nocturnal sleep. METHODS:From 16 subjects, a group called dreamers was formed, and the other 16 individuals were grouped as non-dreamers. Polygraphic recordings lasting 30 min were done at a fixed ti...
journal_title：Archives of medical research
pub_type： 临床试验,杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：BACKGROUND AND AIMS:This study is concerned with the expressions of growth-associated protein-43 (GAP-43) mRNA and protein in the anterior horn of the spinal cord after brachial plexus injury. METHODS:Animals were killed 1, 7, 14 days after injury and were divided into three injury groups: group 1, right C(7) ventral ...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2010.10.005
更新日期：2010-10-01 00:00:00
abstract：BACKGROUND:During congestive heart failure, desensitization of beta-adrenoceptors is related to a lower adrenergic responsiveness in the heart; little is known about alpha 1-adrenoceptors in the vasculature under this condition. We evaluated alpha 1D-adrenoceptor response in aorta and carotid arteries in a model of con...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/s0188-0128(99)00033-0
更新日期：1999-07-01 00:00:00
abstract：BACKGROUND AND AIMS:T-helper (Th) cells involved in the pathogenesis of multiple sclerosis (MS) represent a functionally heterogeneous population defined by their cytokine secretion profile. The effects of immunotherapeutic drugs on the cytokine network are still not fully clarified. This study aimed to investigate ser...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2014.08.001
更新日期：2014-08-01 00:00:00
abstract：:Proteolytic activity was measured in lysates of four axenic and five xenic cultures of different strains of pathogenic E. histolytica, and in five non-pathogenic strains ("E. dispar") growing in xenic culture. There was no significant difference in total proteolytic activity, using either gelatin or albumin as substra...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:Scorpion venom contains different types of peptides toxic to a variety of organisms whose molecular targets have been described as mainly ion-channels of excitable cells where they cause impairment of function. Based on mouse, cricket, and crustacean bioassays, specific toxins for each group of animals have been found...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/s0188-4409(02)00370-3
更新日期：2002-07-01 00:00:00
abstract：BACKGROUND AND AIMS:We tested the effects of recombinant insulin-like growth factor-I (IGF-I) in an adipocyte model of HIV lipodystrophy and in an open label study on body composition and metabolism in patients with HIV lipodystrophy. METHODS:The effects of IGF-I on ritonavir-induced adipocyte cell death were studied ...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2013.06.001
更新日期：2013-07-01 00:00:00
abstract：BACKGROUND:It is well documented that Giardia duodenalis undergoes surface antigenic variation both in vivo and in vitro. Proteins involved have been characterized and referred to as VSP (variable surface protein). METHODS:Two cloned cDNA inserts of 0.45 and 1.95 kb were obtained from G. duodenalis expression library ...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2003.12.005
更新日期：2004-05-01 00:00:00
abstract：BACKGROUND AND AIMS:Few studies have described the association between hepcidin levels and cardiometabolic risk in the general population and more so by considering robust adjustment for confounding factors. Therefore, the aim of the present study was to investigate the associations between circulating hepcidin and ant...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2015.07.007
更新日期：2015-08-01 00:00:00
abstract：:Type 2 diabetes (T2D) is a complex disease caused by the interaction of genetic and environmental factors. In this regard, it has been demonstrated that Hispanics have a greater susceptibility to developing complex diseases like T2D, which has been attributed to their Amerindian component. Mexico has a wide population...
journal_title：Archives of medical research
pub_type： 杂志文章,评审
doi：10.1016/j.arcmed.2016.12.007
更新日期：2016-10-01 00:00:00
abstract：:A prospective study to evaluate the role of vitamin C and cervico-vaginal infection in the premature rupture of amniotic membranes (PROM) was designed. The leukocyte vitamin C levels of 44 pregnant women that did not consume vitamin supplements was evaluated at weeks 20, 28 and 32 of pregnancy. On each evaluation the ...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:High-risk-type human papillomavirus DNA sequences are found in a high percentage of carcinomas from the uterine-cervix, with the viral E1-E2 gene region usually disrupted and the E6 and E7 oncoproteins consistently expressed. The E2 protein is known to repress early transcription from genital HPV promoters having a pr...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：
更新日期：1996-10-01 00:00:00
abstract：BACKGROUND:Patients suffering from hypothyroidism tend to develop diastolic hypertension. 5-Hydroxytryptamine (5-HT) is an amine that contributes to the maintenance of the blood pressure through central and peripheral 5-HT receptors. Curiously, the hypothyroidism alters the density of the 5-HT receptors in rodent brain...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2020.03.013
更新日期：2020-05-01 00:00:00
abstract：:This study evaluated the attachment, chemoattractive, proliferative and mineralization inductive potential of a bovine cementum extract (CPE) on newborn murine dental follicle cells (MDFC) in vitro. Cementum extract was partially purified by DEAE-cellulose chromatography. A band representing an M(r) of 55,000 was exci...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：
更新日期：1997-10-01 00:00:00
abstract：:Hepatitis C represents more than 35% of liver transplant candidates worldwide. Meanwhile, hepatitis B continues to be an important cause of end-stage liver disease and hepatocellular carcinoma in Asia and Africa. Recurrent viral liver disease is a significant event after liver transplantation and continues to be one o...
journal_title：Archives of medical research
pub_type： 杂志文章,评审
doi：10.1016/j.arcmed.2006.09.003
更新日期：2007-08-01 00:00:00
abstract：BACKGROUND:Chromosomal abnormalities have important diagnostic and prognostic significance in acute lymphoblastic leukemia (ALL). The purpose of this study was to define and classify the frequency and type of chromosomal abnormalities among newly diagnosed children with ALL and compare the results with those reported f...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2006.10.006
更新日期：2007-04-01 00:00:00
abstract：:Nicorandil, the first clinically applied ATP-sensitive K+ channel (K+ATP) opener with nitrate property, has demonstrated cardioprotective effects in patients with multiples of heart diseases. However, it is unknown whether nicorandil has effects on left ventricular (LV) remodeling in rats with ischemic heart failure a...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2018.12.006
更新日期：2018-11-01 00:00:00
abstract：:During the past few years, an increasing set of evidence has supported the major role of deregulation of the interaction patterns between glial cells and neurons in the pathway toward neuronal degeneration. Neurons and glial cells, together with brain vessels, constitute an integrated system for brain function. Inflam...
journal_title：Archives of medical research
pub_type： 杂志文章,评审
doi：10.1016/j.arcmed.2007.10.001
更新日期：2008-01-01 00:00:00
abstract：BACKGROUND:Selenoproteins S (SELS or VIMP) may regulate cytokine production, and thus play a key role in the control of the inflammatory response. METHODS:This study consisted of 136 Iranian patients with cardiovascular disease (65 MetS-affected and 71 MetS un-affected individuals) in the selengene study. Expression o...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2020.05.005
更新日期：2020-08-01 00:00:00
abstract：:A model of BALB/c mice was developed to determine the effects of zinc supplements on immune responses during certain stages of ontogeny. The mice received zinc acetate in drinking water concentrations of 500 and 1000 mg/l during the periods of gestation, lactation and postweaning. The sequence employed in this study w...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
abstract：BACKGROUND AND AIMS:Monocyte chemotactic protein-1 (MCP-1) gene polymorphisms play important roles in regulating immunological reactions and may be associated with pulmonary tuberculosis. However, the relationship between the MCP-1 -2518 gene polymorphism and susceptibility to spinal tuberculosis remains unknown. We un...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2013.12.007
更新日期：2014-02-01 00:00:00
abstract：:Implantation is a complex spatio-temporal interaction between the genotypically different embryo and the mother. Success of this event requires the synchronization of development and effective biochemical communications from both sides. Chorionic gonadotropin (CG), which is a major embryonic signal in the primate, is ...
journal_title：Archives of medical research
pub_type： 杂志文章,评审
doi：10.1016/s0188-4409(01)00330-7
更新日期：2001-11-01 00:00:00
abstract：BACKGROUND:The aim of the present study was to determine whether newborn birth weights were associated with insulin-like growth factor I (IGF-I), zinc, and leptin in preeclamptic and normotensive pregnancies. METHODS:This study was done cross-sectionally and included 15 women with moderate preeclampsia (PE group) and ...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/s0188-4409(01)00364-2
更新日期：2002-01-01 00:00:00
