Abstract:
:The ability of synthetic metalloporphyrins to suppress heme oxygenase activity and bilirubin formation has recently become of considerable clinical and experimental interest for suppression of jaundice in humans, including neonatal hyperbilirubinemia. The present investigation compares the biochemical effects of Sn- and Zn-protoporphyrins on the predominant heme oxygenase isozyme present in the brain (HO-2) at activity, protein, and transcript levels and describes the ability of Sn-protoporphyrin to adversely affect this isozyme. Specifically, 6 h after a modest dose (50 mumol/kg, i.v.) of Sn-protoporphyrin, heme oxygenase activity in rat brain was nearly undetectable. In addition, as revealed by Western blot analysis, HO-2 protein level was decreased by 20% and the electrophoretic behavior of the protein in the microsomal membranes was altered. Moreover, the activity of NADPH-cytochrome P-450 reductase, which is required for the oxidation of heme molecule, was markedly decreased (60% of control). Western immunoblot analysis revealed also a pronounced decrease in the reductase protein level. The inducible form of heme oxygenase, HO-1, was not detectable by immunoblotting in brain microsomes of either control or Sn-protoporphyrin-treated animals. Northern blot analyses did not reveal decreases in the levels of the single HO-1 mRNA (1.8 kb) or the two HO-2 transcripts (1.3 and 1.9 kb), suggesting that Sn-protoporphyrin mediates its effects on heme oxygenase isozymes at the protein level. Zn-protoporphyrin, on the other hand, had no deleterious effect on brain parameters presently investigated.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Mark JA,Maines MDdoi
10.1203/00006450-199209000-00016subject
Has Abstractpub_date
1992-09-01 00:00:00pages
324-9issue
3eissn
0031-3998issn
1530-0447journal_volume
32pub_type
杂志文章abstract::Recent data suggest that umbilical venous perfusion of the fetal liver has an important influence on fetal growth and postnatal liver function, and that maternal factors in late pregnancy modify this circulation. In a longitudinal study of 160 low-risk pregnancies, we determined how umbilical and portal venous blood f...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/pdr.0b013e318163a1de
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abstract::Zidovudine (azidothymidine, AZT) is used in pregnancy to reduce mother to infant transmission of HIV. Understanding the disposition of AZT in the fetus is necessary to optimize therapeutic regimens directed toward the fetus. Recent studies in primates found similar steady-state levels of the glucuronide metabolite of ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199807000-00008
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journal_title:Pediatric research
pub_type: 杂志文章
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更新日期:2003-03-01 00:00:00
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pub_type: 杂志文章
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journal_title:Pediatric research
pub_type: 杂志文章,多中心研究
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更新日期:2017-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-06-01 00:00:00
abstract::To assess the National Institutes of Health extramural activity in the area of pediatric nephrology research, the rate of submission of grant applications to the National Institutes of Health and available indicators of the quality of these applications were analyzed. This was accomplished by utilizing the grant appli...
journal_title:Pediatric research
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更新日期:1985-11-01 00:00:00
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journal_title:Pediatric research
pub_type: 杂志文章
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更新日期:1982-07-01 00:00:00
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journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198506000-00016
更新日期:1985-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1203/00006450-199908000-00017
更新日期:1999-08-01 00:00:00
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journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198505000-00004
更新日期:1985-05-01 00:00:00
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更新日期:2020-10-02 00:00:00
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doi:10.1203/00006450-198007000-00014
更新日期:1980-07-01 00:00:00
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更新日期:2009-05-01 00:00:00
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journal_title:Pediatric research
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更新日期:2004-10-01 00:00:00
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更新日期:2005-05-01 00:00:00
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更新日期:2010-11-01 00:00:00
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更新日期:2020-08-01 00:00:00
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pub_type: 杂志文章
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