Abstract:
:We have previously demonstrated that HIV envelope gp41 binding to specific antibodies decreases after preincubation of fluid-phase gp41 in normal human serum. This inhibition is proven to be mediated by the classical complement pathway. In this study recombinant gp41 (rgp41) and/or synthetic peptides were preadsorbed to solid phase, and then complement (normal human serum/heated human serum/purified Clq/heated Clq) and anti-gp41 antibodies were added either after each other or simultaneously, and the amounts of bound antibody, and deposited C3b, C4b and Clq were measured. Complement-dependent inhibition of antibody binding to solid-phase rgp41 was found, and Clq seems to be at least partially responsible for this phenomenon. Heating of Clq did not affect this process. Higher amounts of anti-gp41 antibodies significantly and dose-dependently enhanced C4b and C3b fixation to solid-phase rgp41. In the case of synthetic peptides corresponding to the immunodominant region of gp41, significant antibody binding to the solid-phase peptides was also detected, and pretreatment of peptides preadsorbed to solid phase with normal human serum almost totally abolished the antibody binding.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Hidvégi T,Prohászka Z,Ujhelyi E,Thielens NM,Dierich MP,Hampl H,Arlaud G,Nagy K,Füst Gdoi
10.1111/j.1365-2249.1993.tb08223.xsubject
Has Abstractpub_date
1993-12-01 00:00:00pages
490-3issue
3eissn
0009-9104issn
1365-2249journal_volume
94pub_type
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