Abstract:
:In order to determine the extent of the repertoire of the immunoglobulin light chain V-region locus (Igl-V) in the laboratory rat (Rattus norvegicus), we constructed a specifically primed cDNA library from lipopolysaccharide-stimulated DA strain rat spleens. The library was screened with a rat Igl-C2-specific probe, and 33 clones containing identifiable V regions were sequenced, of which 19 sequences are presented here. In addition to one sequence (Igl-V1) which was already known, and is closely related to the two known mouse V lambda gene segments, clones encoding representatives of three new, distantly related, rat Igl-V subfamilies were found, namely Igl-V2, Igl-V3, and Igl-V4. At least two of these sub-families, Igl-V2 and Igl-V3, contain multiple members as well as restriction fragment length polymorphism variants, indicating the presence of at least 10-15 Igl-V gene segments (including some pseudogenes) in the rat genome. An additional ten clones contained no rearranged V region, although they showed a correct J-C splice, suggesting the presence of cryptic transcriptional promoters between J lambda and the 3'-most Igl-V gene segment. Phylogenetic tree reconstruction based on amino acid sequence alignments showed at least three of the four rat Igl-V sequences clustering with distinct human Igl-V genes. Thus, although rats express lambda-bearing Ig at levels no higher than mice, the rat Igl-V locus is considerably more complex than that of laboratory mice, and its diversity reflects the products of gene duplications which predate the time of primate/rodent divergence.
journal_name
Immunogeneticsjournal_title
Immunogeneticsauthors
Aguilar BA,Gutman GAdoi
10.1007/BF00223543subject
Has Abstractpub_date
1992-01-01 00:00:00pages
39-48issue
1eissn
0093-7711issn
1432-1211journal_volume
37pub_type
杂志文章相关文献
IMMUNOGENETICS文献大全abstract::The anti-HLA-DQ3 monoclonal antibodies (mAb) KS13, SO1, SO2, SO3, SO4, and SO5 recognize spatially close but distinct antigenic determinants, since they crossinhibit each other in their binding to HLA-DQ3 antigens, but do not share idiotopes recognized in their antigen combining site by syngeneic and anti-id antisera ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00178583
更新日期:1995-01-01 00:00:00
abstract::The immune capacity of young and adult axolotls (Ambystoma mexicanum) was evaluated by examining the combinatorial and junctional diversity of the VH chain. A large number of VDJ rearrangements isolated from 2.5-, 3.5-, 10-, and 24-month-old animals were sequenced. Six JH segments were identified with the canonical st...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510050294
更新日期:1997-01-01 00:00:00
abstract::The serological reactivities of HLA-A3, -B7, and -CW3 heavy chains associated with either mouse, bovine, or human beta-2-microglobulin (beta 2m) and expressed on the surface of transfected mouse fibroblasts were analyzed. All reactivities associated with one cluster (defined by monoclonal antibody W6/32) of antigenic ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00430798
更新日期:1985-01-01 00:00:00
abstract::As part of an investigation of the immune system in the developing zebrafish, Danio rerio, we cloned and characterized a genomic V(H) segment and a number of cDNAs encoding IgM heavy chains. The genomic V(H) has the characteristic features of V(H) in other vertebrates, including a leader segment interrupted by a short...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510000255
更新日期:2000-11-01 00:00:00
abstract::Drosophila larvae react against eggs from the endoparasitoid wasp Leptopilina boulardi by surrounding them in a multilayered cellular capsule. Once a wasp egg is recognized as foreign, circulating macrophage-like cells, known as plasmatocytes, adhere to the invader. After spreading around the wasp egg, plasmatocytes f...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-011-0571-3
更新日期:2012-02-01 00:00:00
abstract::Cattle in Africa are a genetically diverse population that has resulted from successive introduction of Asian Bos indicus and European B. taurus cattle. However, analysis of mitochondrial genetic diversity in African cattle identified three lineages, one associated with Asian B. indicus, one with European B. taurus, a...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510000276
更新日期:2001-01-01 00:00:00
abstract::Cytotoxic CD8+ T-cell immunosurveillance for intracellular pathogens, such as viruses, is controlled by classical major histocompatibility complex (MHC) class Ia molecules, and ideally, these antiviral T-cell populations are defined by the specific peptide and restricting MHC allele. Surprisingly, despite the utility ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-013-0711-z
更新日期:2013-09-01 00:00:00
abstract::In this study we analyzed fibroblasts derived from an MHC class II deficiency patient (type III bare lymphocyte syndrome). Northern blot analysis showed that upon induction with IFN-gamma these fibroblasts did not express HLA class II genes and displayed a strongly reduced level of HLA class I gene expression when com...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00172153
更新日期:1995-01-01 00:00:00
abstract::Hepatic granulomatous lesions were induced in mice by a single intraperitoneal injection of 3 mg of disrupted Streptococcus pyogenes cell-wall material. Mice carrying the H-2b or H-2k haplotypes were highly susceptible to the induction and three weeks after the injection produced numerous granulomas. In contrast, mice...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00209289
更新日期:1992-01-01 00:00:00
abstract::Genetic effects on spontaneous autoimmune thyroiditis in chickens were assessed by measuring phenotypic symptoms, the titer of circulating antibody to thyroglobulin, and the pathological change in the thyroids of young chicks. One or more loci within the B complex (the major histocompatibility complex of the chicken) ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF01561675
更新日期:1981-01-01 00:00:00
abstract::Rh and its homologous Rh50 gene products are considered to form heterotetramers on erythrocyte membranes. Rh protein has Rh blood group antigen sites, while Rh50 protein does not, and is more conserved than Rh protein. We previously determined both Rh and Rh50 gene cDNA coding regions from mouse and rat, and carried o...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510000202
更新日期:2000-08-01 00:00:00
abstract::Here we describe the Immunogenetic Management Software (IMS) system, a novel web-based application that permits multiplexed analysis of complex immunogenetic traits that are necessary for the accurate planning and execution of experiments involving large animal models, including nonhuman primates. IMS is capable of ho...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-011-0587-8
更新日期:2012-04-01 00:00:00
abstract::Rheumatoid arthritis (RA) is a chronic autoimmune disease affecting 0.5-1% of the population worldwide. The disease has a heterogeneous character, including clinical subsets of anti-citrullinated protein antibody (ACPA)-positive and APCA-negative disease. Although the pathogenesis of RA is poorly understood, progress ...
journal_title:Immunogenetics
pub_type: 杂志文章,评审
doi:10.1007/s00251-011-0528-6
更新日期:2011-08-01 00:00:00
abstract::The KIR gene cluster exhibits a high degree of polymorphism in terms of gene content as well as allelic polymorphism, and data suggest that it is evolving rapidly. The KIR3DL1 locus is one of the most polymorphic loci within this cluster and is unique in that it encodes an activating receptor KIR3DS1, as well as multi...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-007-0240-8
更新日期:2007-10-01 00:00:00
abstract::The Hawaiian monk seal population has experienced precipitous declines in the last 50 years. In this study, we provide evidence that individuals from remaining endangered population exhibit alarming uniformity in class I major histocompatibility (MHC) genes. The peripheral blood leukocyte-derived mRNA of six captive a...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-005-0069-y
更新日期:2006-04-01 00:00:00
abstract::The recombination activating genes RAG-1 and RAG-2 are adjacent genes that act synergistically to activate variable-diversity-joining (V(D)J) recombination. Southern analysis of hybrid cell lines derived from patients with the Wilms tumor-aniridia-genitourinary defects-mental retardation (WAGR) syndrome and from mutag...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00189518
更新日期:1992-01-01 00:00:00
abstract::Using cDNA hybridization selection techniques, we identified seven new genes in a 280 kilobase YAC covering the HLA-F locus. The new genes were mapped back to the YAC by a combination of optical restriction mapping and pulse field gel electrophoresis. Northern analysis of individual clones demonstrated the presence of...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF02660056
更新日期:1996-01-01 00:00:00
abstract::Human Ia(-like) specificities controlled by gene loci other than HLA-DR were searched for at the molecular level in cells of human B-cell-type cell lines which carry two established DR specificities. Chevalier cells of DRw3 and 7 and U698M cells of DRw2 and 4 were used. Their Ia molecules were partially purified, radi...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF01561564
更新日期:1980-01-01 00:00:00
abstract::Control of HIV-1 viremia and progression to AIDS has been associated with specific HLA genes. The tumor necrosis factor ( TNF) and the non-classical major histocompatibility (MHC) class I chain-related A ( MICA) genes are located in the genomic segment between the HLA class I and II genes and variants of both genes ha...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-003-0604-7
更新日期:2003-10-01 00:00:00
abstract::Previous studies demonstrated that the diversity of the antibody response of mice to the inulin (In) determinant of bacterial levan is regulated by the gene Spectrotype Regulation 1 ( Sr1). BALB/c mice produce a monoclonal anti-In response as shown by isoelectric focusing analysis. In contrast, the anti-In antibody re...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-003-0555-z
更新日期:2003-05-01 00:00:00
abstract::The N-terminal domain of the chemokine CCL5/regulated upon activation normal T cell expressed and secreted (RANTES) has been shown to be critical for its biological activity on leukocytes. Several N-terminus-modified CCL5/RANTES derivatives, such as N-Terminal truncated CCL5/RANTES, Met-RANTES, and amino-oxypentane (A...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-006-0133-2
更新日期:2006-07-01 00:00:00
abstract::Described here is a new, more efficient method for defining major histocompatibility complex-Y (MHC-Y) genotypes in chickens. The MHC-Y region is genetically independent from the classical MHC (MHC-B) region. MHC-Y is highly polymorphic and potentially important in the genetics of disease resistance. MHC-Y haplotypes ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-020-01166-6
更新日期:2020-07-01 00:00:00
abstract::The organization of the major histocompatibility complex (MHC) class II region in cattle was investigated by Southern blot analysis using human probes corresponding to DO, DP, DQ, and DR genes. Exon-specific probes were also employed to facilitate the assessment of the number of different bovine class II genes. The re...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00351084
更新日期:1988-01-01 00:00:00
abstract::Rearrangements of the immunoglobulin heavy chain joining region (JH) genes occur in some T lymphocytes, probably because the mechanism for assembly of T-cell receptor encoded genes is very similar to that for immunoglobulins. Two such rearrangements described previously represented proper fusion of a DH and JH gene. W...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00345502
更新日期:1988-01-01 00:00:00
abstract::Endemic pemphigus foliaceus (EPF), is an autoimmune disease associated with production of IgG antibodies against epidermal antigens. We have tested 38 patients and 50 control subjects living in endemic areas to investigate whether HLA genes are associated with host factors that determine whether or not exposed individ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00216698
更新日期:1991-01-01 00:00:00
abstract::The CCL4 chemokine is secreted by a variety of cells following stimulation. CCL4 affects several different types of cells that are important for acute inflammatory responses and are critical for the development of specific immune responses to foreign antigens. The human genome contains two genes for the CCL4 chemokine...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-003-0636-z
更新日期:2004-01-01 00:00:00
abstract::The heavy and light chain subunits of the DC1 antigen from several cell lines expressing different DR specificities were compared by two-dimensional (2-D) gel electrophoresis. The DC1 light chains from cell lines typed as DR1, DR2, or DRw6 differed in their isoelectric points. No difference in charge was observed for ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00345970
更新日期:1983-01-01 00:00:00
abstract::The Mep-1 gene on chromosome 17 in mice controls the activity of meprin, a kidney brush border metalloendopeptidase. Most inbred mouse strains of the k haplotype (e.g., CBA, C3H, AKR) are markedly deficient in meprin activity; these mice carry the Mep-1b allele. Mouse strains in which meprin activity levels are normal...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00430310
更新日期:1985-01-01 00:00:00
abstract::The rabbit has long been a model for studies of the immune system. Work using rabbits contributed both to the battle against infectious diseases such as rabies and syphilis, and to our knowledge, of antibodies' structure, function, and regulated expression. With the description of rabbit Ig allotypes, the discovery of...
journal_title:Immunogenetics
pub_type: 杂志文章,评审
doi:10.1007/s00251-011-0533-9
更新日期:2011-07-01 00:00:00
abstract::In order to further our understanding of major histocompatibility complex (MHC) class I gene organization, we began a comparative analysis of the large scale organization of the class I region in diverse haplotypes. For these studies, the MHC in healthy Japanese donors who have the predominant MHC haplotypes and/or HL...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510050252
更新日期:1997-01-01 00:00:00