Effects of dl-praeruptorin A on nucleus factor-kappaB and tumor necrosis factor-alpha expression in ischemia-reperfusion hearts.

abstract：AIM:To study the expressions of vascular endothelial growth factor (VEG F), angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2), Tie-1, and Tie-2 in C57BL/6 mouse brain after permanent focal cerebral ischemia. METHODS:The mRNA levels of VEGF, Ang-1, Ang-2, Tie-1, and Tie-2 were measured by semiquantitative reverse transcri...

journal_title：Acta pharmacologica Sinica

authors： Wang RG,Zhu XZ

更新日期：2002-05-01 00:00:00

abstract：:The chemokine receptor CCR5 is an important anti-HIV (human immunodeficiency virus) drug target owning to its pivotal role in HIV-1 viral entry as a co-receptor. Here, we present a 2.9 Å resolution crystal structure of CCR5 bound to PF-232798, a second-generation oral CCR5 antagonist currently in phase II clinical tri...

journal_title：Acta pharmacologica Sinica

authors： Zhu Y,Zhao YL,Li J,Liu H,Zhao Q,Wu BL,Yang ZL

更新日期：2019-04-01 00:00:00

abstract：AIM:Osteopontin (OPN), a multifunctional protein, has been reported to be protoxicant in acetaminophen hepatotoxicity. In this study, the mechanisms underlying the detrimental role of OPN in acetaminophen toxicity were explored. METHODS:Male C57BL/6 (wild-type, WT) and OPN(-/-) mice were administered with acetaminophe...

journal_title：Acta pharmacologica Sinica

authors： He CY,Liang BB,Fan XY,Cao L,Chen R,Guo YJ,Zhao J

更新日期：2012-08-01 00:00:00

abstract：AIM:To discover novel cell division cycle 25 (CDC25) B inhibitors and elucidate the mechanisms of inhibition in cancer cells. METHODS:Cell growth inhibition was detected by MTT assay, the cell cycle was analyzed by flow cytometry, and protein expression and phosphorylation was examined by Western blot analysis. RESUL...

journal_title：Acta pharmacologica Sinica

authors： Zhou YB,Feng X,Wang LN,Du JQ,Zhou YY,Yu HP,Zang Y,Li JY,Li J

更新日期：2009-09-01 00:00:00

abstract：AIM:Filamin binding LIM protein 1, also known as migfilin, is a skeleton organization protein that binds to mitogen-inducible gene 2 at cell-extracellular matrix adhesions. The aim of this study was to investigate the role of migfilin in cisplatin-induced apoptosis in human glioma cells, to determine the functional dom...

journal_title：Acta pharmacologica Sinica

authors： Fan J,Ou YW,Wu CY,Yu CJ,Song YM,Zhan QM

更新日期：2012-10-01 00:00:00

abstract：AIM:The interaction of anthracycline anticancer drugs with chromatin, nucleosomes and histone H1 has been extensively studied. In the present study, for the first time, we have investigated the binding of anthracycline antibiotic, daunomycin, to free and cross-linked thymus core histones (CL-core) in solution and in th...

journal_title：Acta pharmacologica Sinica

authors： Rabbani A,Abdosamadi S,Sari-Saraf N

更新日期：2007-05-01 00:00:00

abstract：:Cysteine-rich peptides from the venom of cone snails (Conus) target a wide variety of different ion channels. One family of conopeptides, the alpha-conotoxins, specifically target different isoforms of nicotinic acetylcholine receptors (nAChRs) found both in the neuromuscular junction and central nervous system. This ...

journal_title：Acta pharmacologica Sinica

authors： Azam L,McIntosh JM

更新日期：2009-06-01 00:00:00

abstract：:Dry eye disease (DED) is a multifactorial disorder of the tears and ocular surface characterized by manifestations of dryness and irritation. Although the pathogenesis is not fully illuminated, it is recognized that inflammation has a prominent role in the development and deterioration of DED. β-aminoarteether maleate...

journal_title：Acta pharmacologica Sinica

authors： Yang FM,Fan D,Yang XQ,Zhu FH,Shao MJ,Li Q,Liu YT,Lin ZM,Cao SQ,Tang W,He SJ,Zuo JP

更新日期：2020-08-03 00:00:00

abstract：AIM:Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) have been shown to ameliorate cerebral ischemia in animal models. In this study we investigated the effects of hUCB-MSCs on inflammatory responses and neuronal apoptosis during the early stage of focal cerebral ischemia in rabbits. METHODS:Focal cerebra...

journal_title：Acta pharmacologica Sinica

authors： Zhu Y,Guan YM,Huang HL,Wang QS

更新日期：2014-05-01 00:00:00

abstract：AIM:To study and establish a proteome reference map and regulation network of neonatal rat cardiomyocyte. METHODS:Cultured cardiomyocytes of neonatal rats were used. All proteins expressed in the cardiomyocytes were separated and identified by two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assist...

journal_title：Acta pharmacologica Sinica

authors： Li ZJ,Liu N,Han QD,Zhang YY

更新日期：2011-09-01 00:00:00

abstract：AIM:To study the effect of semi-synthesized quercetin water-soluble derivatives sodium quercetin-7-sulfate (SQMS) and disodium quercetin-7,4 -disulfate (SQDS) on recombinant human phosphatidylinositol 3-kinase (PI3-K) p110 beta catalytic subunit. METHODS:Recombinant human PI3-K p110 beta catalytic subunit was expresse...

journal_title：Acta pharmacologica Sinica

authors： Liu W,Liang NC

更新日期：2002-04-01 00:00:00

abstract：AIM:To develop a simple method for monitoring protein localization of epidermal growth factor (EGF) in living cells. METHODS:Enhanced green fluorescent protein (EGFP) was used as an autofluorescent tag to label EGF ligands. SDS-PAGE and Western blot analysis were used to detect the expression of the EGFP-tagged EGF (E...

journal_title：Acta pharmacologica Sinica

authors： Jiang H,Zhang J,Shi BZ,Xu YH,Li ZH,Gu JR

更新日期：2007-01-01 00:00:00

abstract：AIM:To investigate the protective effects of hydrogen sulfide (H2S) against inflammation, oxidative stress and apoptosis in a rat model of resuscitated hemorrhagic shock. METHODS:Hemorrhagic shock was induced in adult male SD rats by drawing blood from the femoral artery for 10 min. The mean arterial pressure was main...

journal_title：Acta pharmacologica Sinica

authors： Xu DQ,Gao C,Niu W,Li Y,Wang YX,Gao CJ,Ding Q,Yao LN,Chai W,Li ZC

更新日期：2013-12-01 00:00:00

abstract：AIM:To investigate whether nimesulide could suppress tumor growth and induce apoptosis in implanted hepatoma mice and to explore the molecular mechanisms. METHODS:Male mice received nimesulide 10 mg/kg, 20 mg/kg, and 40 mg/kg ig daily for 21 d. Electron microscopy (EM), flow cytometry (FCM), DNA ladder, radioimmunoass...

journal_title：Acta pharmacologica Sinica

authors： Li XH,Li JJ,Zhang HW,Sun P,Zhang YL,Cai SH,Ren XD

更新日期：2003-10-01 00:00:00

abstract：:Estrogens are important endocrine hormones that control physiological functions in reproductive organs, and play a pivotal role in the generation and progression of breast cancer. Therapeutic drugs including anti-estrogen and aromatase inhibitors are used to treat patients with breast cancer. The estrogen receptors, E...

journal_title：Acta pharmacologica Sinica

authors： Ikeda K,Horie-Inoue K,Inoue S

更新日期：2015-01-01 00:00:00

abstract：AIM:To compare high K(+ )-induced contraction and optimal resting tension measured by two commonly used techniques of hypertonic and isotonic K(+ ) in aortas with and without adventitial fat from various age rats. METHODS:Three age groups of rats (15, 25, and 62 weeks) were used to prepare thoracic aortic rings in whi...

journal_title：Acta pharmacologica Sinica

authors： Guan YF,Chen RH,Wang P,Qin Y,Su DF,Miao CY

更新日期：2007-05-01 00:00:00

abstract：AIM:The aim of this study was to study the effects of compound FLZ, a novel cyclic derivative of squamosamide from Annona glabra, on brain-derived neurotrophic factor (BDNF)/tropomyosin receptor kinase B (TrkB)/cAMP response element-binding protein (CREB) signaling and neuronal apoptosis in the hippocampus of the amylo...

journal_title：Acta pharmacologica Sinica

authors： Li N,Liu GT

更新日期：2010-03-01 00:00:00

abstract：:Autophagy, a form of cellular self-digestion by lysosome, is associated with various disease processes including cancers, and modulating autophagy has shown promise in the treatment of various malignancies. A number of natural products display strong antitumor activity, yet their mechanisms of action remain unclear. T...

journal_title：Acta pharmacologica Sinica

authors： Jiang SL,Guan YD,Chen XS,Ge P,Wang XL,Lao YZ,Xiao SS,Zhang Y,Yang JM,Xu XJ,Cao DS,Cheng Y

更新日期：2019-07-01 00:00:00

abstract：:The intercellular communication between leukemia cells and bone marrow mesenchymal stem cells (BM-MSCs) plays more important role in chronic myeloid leukemia (CML) than we previously understood. Recently, we found that microvesicles released from human leukemia cell line K562 (K562-MVs) containing BCR-ABL1 mRNA malign...

journal_title：Acta pharmacologica Sinica

authors： Fu FF,Zhu XJ,Wang HX,Zhang LM,Yuan GL,Chen ZC,Li QB

更新日期：2017-11-01 00:00:00

abstract：AIM:The substrate cocktail is frequently used to evaluate cytochrome P450 (CYP) enzyme-mediated drug interactions and potential interactions among the probe substrates. Here, we re-optimized the substrate cocktail method to increase the reliability and accuracy of screening for candidate compounds and expanded the meth...

journal_title：Acta pharmacologica Sinica

authors： Chen ZH,Zhang SX,Long N,Lin LS,Chen T,Zhang FP,Lv XQ,Ye PZ,Li N,Zhang KZ

更新日期：2016-05-01 00:00:00

abstract：AIM:To establish a 96-well plate based kinase assay using a recombinant vascular endothelial growth factor (VEGF) receptor 1 kinase domain protein. METHODS:A human VEGF receptor 1 kinase domain protein was expressed in E coli, and its activity was monitored by its ability of phosphorylating the polyE4Y substrate coate...

journal_title：Acta pharmacologica Sinica

authors： Zhuang SF,Zhou CH,Qian J,Qian Z,Shibuya M,Ye QZ

更新日期：2002-02-01 00:00:00

abstract：AIM:To investigate the effect of Ginkgolide B (GB) on action potential (AP), delayed rectifier potassium current (IK), and L-type calcium current (I(Ca-L)) in guinea pig ventricular myocytes. METHODS:Single ventricular myocytes were isolated by an enzymatic dissociation method. AP, IK, I(Ca-L) were recorded by whole-c...

journal_title：Acta pharmacologica Sinica

authors： Qi XY,Zhang ZX,Xu YQ

更新日期：2004-02-01 00:00:00

abstract：AIM:To develop a robust, cell-based, high-content screening (HCS) assay based on receptor internalization for the identification of novel modulators of the epidermal growth factor receptor (EGFR). METHODS:Agonist-induced receptor internalization is part of the signaling cascade of EGFR. Fluorescent-tagged epidermal gr...

journal_title：Acta pharmacologica Sinica

authors： Wang J,Xie X

更新日期：2007-10-01 00:00:00

abstract：AIM:Pim-2 is a short-lived serine/threonine kinase, which plays a key role in metastasis of breast cancer through persistent activation of STAT3. Although the crystal structure of Pim-2 has been reported, but thus far no specific Pim-2-targeted compounds have been reported. In this study, we identified a novel Pim-2 in...

journal_title：Acta pharmacologica Sinica

authors： Zhao YQ,Yin YQ,Liu J,Wang GH,Huang J,Zhu LJ,Wang JH

更新日期：2016-09-01 00:00:00

abstract：AIM:To determine the active ingredient of Niuchangchih (Antrodia camphorata) responsible for its anti-inflammatory effects and the relevant molecular mechanisms. METHODS:Five major antcins (A, B, C, H, and K) were isolated from fruiting bodies of Niuchangchih. Structural similarity between the antcins and 2 glucocorti...

journal_title：Acta pharmacologica Sinica

authors： Chen YC,Liu YL,Li FY,Chang CI,Wang SY,Lee KY,Li SL,Chen YP,Jinn TR,Tzen JT

更新日期：2011-07-01 00:00:00

abstract：AIM:To examine how the higher expression level of CYP3A4 isoenzyme influenced the cytotoxicity of the antitumor triazoloacridinone derivative C-1305 in Chinese hamster ovary (CHO) cells. METHODS:Three CHO cell lines were examined: wild-type CHO cells; CHO-HR cells with overexpression of human cytochrome P450 reductase...

journal_title：Acta pharmacologica Sinica

authors： Augustin E,Borowa-Mazgaj B,Kikulska A,Kordalewska M,Pawłowska M

更新日期：2013-01-01 00:00:00

abstract：AIM:Pirarubicin (THP) is recently found to be effective in treating patients with advanced, relapsed or recurrent high-grade osteosarcoma. In this study, the effects of THP on the multidrug-resistant (MDR) osteosarcoma cells were assessed, and the underlying mechanisms for the disruption of cell cycle kinetics by THP w...

journal_title：Acta pharmacologica Sinica

authors： Zheng SE,Xiong S,Lin F,Qiao GL,Feng T,Shen Z,Min DL,Zhang CL,Yao Y

更新日期：2012-06-01 00:00:00

abstract：:Neuronal nicotinic acetylcholine receptors are important targets for alcohol reward and dependence. Alcoholism is a serious public health problem and has been identified as the third major cause of preventable mortality in the world. Worldwide, about 2 billion people consume alcohol, with 76.3 million having diagnosab...

journal_title：Acta pharmacologica Sinica

authors： Wu J,Gao M,Taylor DH

更新日期：2014-03-01 00:00:00

abstract：AIM:To make a LQT3 model (one form of the long QT syndromes) and to investigate the effect of mexiletine on LQT3. METHODS:Sea anemone toxin (ATX II) was used to produce the LQT3 model. The Effect of mexiletine on LQT3 was performed on single Na channel, action potential, and electrocardiography in guinea pigs. RESULT...

journal_title：Acta pharmacologica Sinica

authors： Wang HW,Zheng YQ,Yang ZF,Li CZ,Liu YM

更新日期：2003-04-01 00:00:00

abstract：:Midbrain dopaminergic (DA) neurons are governed by an endogenous cholinergic system, originated in the mesopontine nuclei. Nicotine hijacks nicotinic acetylcholine receptors (nAChRs) and interferes with physiological function of the cholinergic system. In this review, we describe the anatomical organization of the cho...

journal_title：Acta pharmacologica Sinica

authors： Xiao C,Zhou CY,Jiang JH,Yin C

更新日期：2020-01-01 00:00:00