Abstract:
:The avian and Nelson Bay reoviruses are two of only a limited number of nonenveloped viruses capable of inducing cell-cell membrane fusion. These viruses encode the smallest known membrane fusion proteins (p10). We now show that a region of moderate hydrophobicity we call the hydrophobic patch (HP), present in the small N-terminal ectodomain of p10, shares the following characteristics with the fusion peptides of enveloped virus fusion proteins: (i) an abundance of glycine and alanine residues, (ii) a potential amphipathic secondary structure, (iii) membrane-seeking characteristics that correspond to the degree of hydrophobicity, and (iv) the ability to induce lipid mixing in a liposome fusion assay. The p10 HP is therefore predicted to provide a function in the mechanism of membrane fusion similar to those of the fusion peptides of enveloped virus fusion peptides, namely, association with and destabilization of opposing lipid bilayers. Mutational and biophysical analysis suggested that the internal fusion peptide of p10 lacks alpha-helical content and exists as a disulfide-stabilized loop structure. Similar kinked structures have been reported in the fusion peptides of several enveloped virus fusion proteins. The preservation of a predicted loop structure in the fusion peptide of this unusual nonenveloped virus membrane fusion protein supports an imperative role for a kinked fusion peptide motif in biological membrane fusion.
journal_name
J Viroljournal_title
Journal of virologyauthors
Shmulevitz M,Epand RF,Epand RM,Duncan Rdoi
10.1128/jvi.78.6.2808-2818.2004subject
Has Abstractpub_date
2004-03-01 00:00:00pages
2808-18issue
6eissn
0022-538Xissn
1098-5514journal_volume
78pub_type
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pub_type: 杂志文章
doi:10.1128/JVI.70.6.3478-3487.1996
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pub_type: 杂志文章
doi:10.1128/JVI.68.6.3943-3954.1994
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.2.552-557.1988
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pub_type: 杂志文章
doi:10.1128/JVI.18.2.627-635.1976
更新日期:1976-05-01 00:00:00
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更新日期:2014-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
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更新日期:2001-07-01 00:00:00
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journal_title:Journal of virology
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更新日期:2013-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1993-12-01 00:00:00
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更新日期:2013-08-01 00:00:00