Determination of the stoichiometry of protein complexes using liquid chromatography with fluorescence and mass spectrometric detection of fluorescently labeled proteolytic peptides.

abstract：:The large interactive surfaces of nanoparticles (NPs) increase the opportunities to develop NPs for vascular targeting. Proteomic analysis of endothelial cells exposed to NPs reveals the cellular response and turns the focus into the impairment of the endothelial permeability. Here, quantitative proteomics and transcr...

journal_title：Proteomics

authors： Kuruvilla J,Bayat N,Cristobal S

更新日期：2019-03-01 00:00:00

abstract：:Precise protein quantification is essential in comparative proteomics. Currently, quantification bias is inevitable when using proteotypic peptide-based quantitative proteomics strategy for the differences in peptides measurability. To improve quantification accuracy, we proposed an "empirical rule for linearly correl...

journal_title：Proteomics

authors： Liu K,Zhang J,Fu B,Xie H,Wang Y,Qian X

更新日期：2014-07-01 00:00:00

abstract：:Although the importance of the extracellular signal-regulated kinase (ERK) pathway in regulating the transition from G1 to S has been extensively studied, its role during the G2/M transition is less well understood. Previous reports have shown that inhibition of the ERK pathway in mammalian cells delays entry as well ...

journal_title：Proteomics

authors： Roberts EC,Hammond K,Traish AM,Resing KA,Ahn NG

更新日期：2006-08-01 00:00:00

abstract：:Mycobacterium avium subsp. paratuberculosis has long been recognized as the causative agent of Johne's disease, a chronic inflammatory intestinal disease of sheep, cattle and other ruminants. Mycobacterial cells are extremely hardy, and proteomic analyses require the use of harsh conditions to effect their disruption....

journal_title：Proteomics

authors： Lanigan MD,Vaughan JA,Shiell BJ,Beddome GJ,Michalski WP

更新日期：2004-04-01 00:00:00

abstract：:The discovery of PTMs in proteins by MS requires nearly complete sequence coverage of the detected proteolytic peptides. Unfortunately, mass spectrometric analysis of the desired sequence fragments is often impeded due to low ionization efficiency and/or signal suppression in complex samples. When several lysine resid...

journal_title：Proteomics

authors： Markoutsa S,Bahr U,Papasotiriou DG,Häfner AK,Karas M,Sorg BL

更新日期：2014-03-01 00:00:00

abstract：:Proteases are critical in many physiological processes and the human genome encodes for 566 predicted proteolytic enzymes. Therefore, there is great interest in identifying and characterizing physiologic protease-substrate relationships. The coagulation cascade is a well-described network of serine proteases. However,...

journal_title：Proteomics

authors： Niessen S,Hoover H,Gale AJ

更新日期：2011-06-01 00:00:00

abstract：:Toxoplasma gondii infects a wide range of hosts worldwide, including humans and domesticated animals causing toxoplasmosis disease. Recently, exosomes, small extracellular vesicles (EV) that contain nucleic acids, proteins, and lipids derived from their original cells were linked with disease protection. The effect of...

journal_title：Proteomics

authors： Wowk PF,Zardo ML,Miot HT,Goldenberg S,Carvalho PC,Mörking PA

更新日期：2017-08-01 00:00:00

abstract：:We have combined several key sample preparation steps for the use of a liquid matrix system to provide high analytical sensitivity in automated ultraviolet -- matrix-assisted laser desorption/ionisation -- mass spectrometry (UV-MALDI-MS). This new sample preparation protocol employs a matrix-mixture which is based on ...

journal_title：Proteomics

authors： Cramer R,Corless S

更新日期：2005-02-01 00:00:00

abstract：:Glycosylation is a widespread PTM of proteins; the carbohydrate moieties provide various functional, immunological and structural aspects of both eukaryotic and prokaryotic glycoproteins. Traditional strategies used to analyse glycoprotein O-glycans involve glycoprotein isolation, followed by glycan release using solu...

journal_title：Proteomics

authors： Taylor AM,Holst O,Thomas-Oates J

更新日期：2006-05-01 00:00:00

abstract：:Off-line coupling of capillary IEF (CIEF) with matrix-assisted laser desorption/ionization mass spectrometry was utilized for the analysis of human blood serum. Serum proteins were initially separated by CIEF, and fractions of the isoelectric separation were eluted sequentially to a MALDI-TOF MS sample target. During ...

journal_title：Proteomics

authors： Crowley TA,Hayes MA

更新日期：2005-09-01 00:00:00

abstract：:Ovarian cancer is one of the leading causes of mortality by gynecological cancer. Despite good response to surgery and initial chemotherapy, essentially based on cisplatin (cis-diamino-dichloro-platinum(II) (CDDP)) compounds, frequent recurrences with chemoresistance acquisition are responsible for poor prognosis. Sev...

journal_title：Proteomics

authors： Le Moguen K,Lincet H,Marcelo P,Lemoisson E,Heutte N,Duval M,Poulain L,Vinh J,Gauduchon P,Baudin B

更新日期：2007-11-01 00:00:00

abstract：:The determination of possible biomarkers in nasal secretion of healthy subjects can have a role in early diagnosis of diseases such as rhinosinusitis. For this purpose, nasal lavage fluids (NLFs) from ten volunteers, collected before and after they had been submitted to nasal provocations, were investigated. Separatio...

journal_title：Proteomics

authors： Casado B,Pannell LK,Iadarola P,Baraniuk JN

更新日期：2005-07-01 00:00:00

abstract：:The protein components of urine are useful indicators of renal function and human health in general. Urine samples are easily attainable making them ideal substrates for biomarker research. Analysis of the urine proteome however, has been hindered by the great variability of the urine specimens, and the presence of va...

journal_title：Proteomics

authors： Zerefos PG,Vougas K,Dimitraki P,Kossida S,Petrolekas A,Stravodimos K,Giannopoulos A,Fountoulakis M,Vlahou A

更新日期：2006-08-01 00:00:00

abstract：:The availability of completed genome sequences of several eukaryotic and prokaryotic species has shifted the focus towards the identification and characterization of all gene products that are expressed in a given organism. In order to cope with the huge amounts of data that have been provided by large-scale sequencin...

journal_title：Proteomics

authors： Auerbach D,Thaminy S,Hottiger MO,Stagljar I

更新日期：2002-06-01 00:00:00

abstract：:A two-dimensional liquid-phase separation scheme coupled with mass spectrometry (MS) is presented for proteomic analysis of cell lysates from normal and malignant breast epithelial cell lines. Liquid-phase separations consist of isoelectric focusing as the first dimension and nonporous silica reverse-phase high-perfor...

journal_title：Proteomics

authors： Hamler RL,Zhu K,Buchanan NS,Kreunin P,Kachman MT,Miller FR,Lubman DM

更新日期：2004-03-01 00:00:00

abstract：:An improved method for peptide sequencing based on acetylation/deuteroacetylation in conjunction with ESI MS is introduced. Derivatization with a 1:1 mixture of acetic anhydride and deuterated acetic anhydride incorporates a stable isotope label into the analyzed molecule. This approach has been initially applied to F...

journal_title：Proteomics

authors： Noga MJ,Lewandowski JJ,Suder P,Silberring J

更新日期：2005-11-01 00:00:00

abstract：:Anaeromyxobacter dehalogenans is a microaerophilic member of the delta-proteobacteria which is able to utilize a wide range of electron acceptors, including halogenated phenols, U(VI), Fe(III), nitrate, nitrite, oxygen and fumarate. To date, the knowledge regarding general metabolic activities of this ecologically rel...

journal_title：Proteomics

authors： Chao TC,Kalinowski J,Nyalwidhe J,Hansmeier N

更新日期：2010-04-01 00:00:00

abstract：:Targeted quantitative proteomic analyses aim at systematically measuring the abundance of proteins in large sets of samples, without biases or missing values. One typical implementation is the verification of biomarker candidates in bodily fluids, which measures extended lists of validated transitions using triple qua...

journal_title：Proteomics

authors： Lesur A,Domon B

更新日期：2015-03-01 00:00:00

abstract：:Despite increasing evidence of behavioural manipulation of their vectors by pathogens, the underlying mechanisms causing infected vectors to act in ways that benefit pathogen transmission remain enigmatic in most cases. Here, 2-D DIGE coupled with MS were employed to analyse and compare the head proteome of mosquitoes...

journal_title：Proteomics

authors： Lefevre T,Thomas F,Schwartz A,Levashina E,Blandin S,Brizard JP,Le Bourligu L,Demettre E,Renaud F,Biron DG

更新日期：2007-06-01 00:00:00

abstract：:Leishmaniasis is a zoonotic disease caused by the species of the genus Leishmania, flagellated protozoa that multiply inside mammalian macrophages and are transmitted by the bite of the sandfly. The disease is widespread and due to the lack of fully effective treatment and vaccination the search for new drugs and immu...

journal_title：Proteomics

authors： Dea-Ayuela MA,Rama-Iñiguez S,Bolás-Fernández F

更新日期：2006-07-01 00:00:00

abstract：:RADARS, a rapid, automated, data archiving and retrieval software system for high-throughput proteomic mass spectral data processing and storage, is described. The majority of mass spectrometer data files are compatible with RADARS, for consistent processing. The system automatically takes unprocessed data files, iden...

journal_title：Proteomics

authors： Field HI,Fenyö D,Beavis RC

更新日期：2002-01-01 00:00:00

abstract：:Exercise training has been recommended as a nonpharmacological strategy for the prevention and attenuation of skeletal muscle atrophy in distinct pathophysiological conditions. Despite the well-established phenotypic alterations, the molecular mechanisms underlying exercise-induced skeletal muscle remodeling are poorl...

journal_title：Proteomics

authors： Padrão AI,Ferreira R,Amado F,Vitorino R,Duarte JA

更新日期：2016-03-01 00:00:00

abstract：:GlycoMod (http://www.expasy.ch/tools/glycomod/) is a software tool designed to find all possible compositions of a glycan structure from its experimentally determined mass. The program can be used to predict the composition of any glycoprotein-derived oligosaccharide comprised of either underivatised, methylated or ac...

journal_title：Proteomics

authors： Cooper CA,Gasteiger E,Packer NH

更新日期：2001-02-01 00:00:00

abstract：:The use of isobaric tags such as iTRAQ allows the relative and absolute quantification of hundreds of proteins in a single experiment for up to eight different samples. More classical techniques such as 2-DE can offer a complimentary approach for the analysis of complex protein samples. In this study, the proteomes of...

journal_title：Proteomics

authors： Mehaffy C,Hess A,Prenni JE,Mathema B,Kreiswirth B,Dobos KM

更新日期：2010-05-01 00:00:00

abstract：:The 26S proteasome is a multicatalytic protease complex that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core (the 20S proteasome) as well as regulatory particles, which contain six ATPase (Rpt) subunits involved in unfolding and translocation of substrates to the catalytic chambe...

journal_title：Proteomics

authors： Kimura Y,Kurata Y,Ishikawa A,Okayama A,Kamita M,Hirano H

更新日期：2013-11-01 00:00:00

abstract：:Head and neck cancers, including oral squamous cell carcinoma (OSCC), are the sixth most common malignancy in the world and are characterized by poor prognosis and a low survival rate. Saliva is oral fluid with intimate contact with OSCC. Besides non-invasive, simple, and rapid to collect, saliva is a potential source...

journal_title：Proteomics

authors： Kawahara R,Bollinger JG,Rivera C,Ribeiro AC,Brandão TB,Paes Leme AF,MacCoss MJ

更新日期：2016-01-01 00:00:00

abstract：:Bottom-up proteomics is a mass spectrometric (MS)-based approach for the characterization of peptides obtained from in-solution protein digestion. MS is favored over other methods for peptide and protein analysis because of its better sensitivity and high throughput. Inorganic ions and surfactants present in the sampl...

journal_title：Proteomics

authors： Tubaon RM,Haddad PR,Quirino JP

更新日期：2017-10-01 00:00:00

abstract：:The authors present pathwayPCA, an R/Bioconductor package for integrative pathway analysis that utilizes modern statistical methodology, including supervised and adaptive, elastic-net, sparse principal component analysis. pathwayPCA can be applied to continuous, binary, and survival outcomes in studies with multiple c...

journal_title：Proteomics

authors： Odom GJ,Ban Y,Colaprico A,Liu L,Silva TC,Sun X,Pico AR,Zhang B,Wang L,Chen X

更新日期：2020-11-01 00:00:00

abstract：:Peripheral nerve injury may lead to neuroadaptive changes of cellular signals in spinal cord that are thought to contribute to central mechanisms underlying neuropathic pain. Here we used a 2-DE-based proteomic technique to determine the global expression changes of synaptosome-associated proteins in spinal cord dorsa...

journal_title：Proteomics

authors： Singh OV,Yaster M,Xu JT,Guan Y,Guan X,Dharmarajan AM,Raja SN,Zeitlin PL,Tao YX

更新日期：2009-03-01 00:00:00

abstract：:We describe the first phosphoproteome of the model filamentous fungus Aspergillus nidulans. Phosphopeptides were enriched using titanium dioxide, separated using a convenient ultra-long reverse phase gradient, and identified using a "high-high" strategy (high mass accuracy on the parent and fragment ions) with higher-...

journal_title：Proteomics

authors： Ramsubramaniam N,Harris SD,Marten MR

更新日期：2014-11-01 00:00:00