Abstract:
BACKGROUND:The severe acute respiratory syndrome (SARS) was first described in February 2003. Close contact with symptomatic patients appears to be the main route of transmission, whereas blood transfusion transmission could not be ruled out. STUDY DESIGN AND METHODS:A SARS coronavirus (SARS-CoV) detection kit developed by C. Drosten (Bernhard Nocht Institute, BNI) was used to amplify SARS-CoV sequences from blood donor samples. We tested 31,151 blood donor samples in minipools of up to 96 samples. To validate the sensitivity of the assay, routine donor minipools (88 +/- 8 samples per pool) were spiked with plasma of an imported case of SARS or of a subsequently infected contact person, respectively. Gamma-irradiated cell culture supernatants of Vero E6 cells, infected with SARS-CoV, were used as positive controls. RESULTS:None of 31,151 blood donors were positive for the presence of SARS. Two 96-member plasma pools that were each spiked with 100 microL of plasma of the German index patient or his wife, respectively, were positive. Overall, 0.85 percent of test results had to be considered invalid owing to negative internal controls. CONCLUSION:A real-time CoV PCR test is able to detect SARS-CoV in viremic blood donor samples even in the beginning of the disease when patients present minor clinical symptoms. Thus the assay could potentially help to prevent transfusion-associated SARS-CoV transmissions.
journal_name
Transfusionjournal_title
Transfusionauthors
Schmidt M,Brixner V,Ruster B,Hourfar MK,Drosten C,Preiser W,Seifried E,Roth WKdoi
10.1111/j.1537-2995.2004.03269.xsubject
Has Abstractpub_date
2004-04-01 00:00:00pages
470-5issue
4eissn
0041-1132issn
1537-2995pii
TRF03269journal_volume
44pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract::Quality control to detect inadequately deglycerolized red blood cells can be easily and inexpensively accomplished by suspending the deglycerolized cells in either recipient serum or normal saline in the same way in which the routine crossmatch is performed. In vitro hemolysis is readily and consistently apparent when...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1982.22382224955.x
更新日期:1982-05-01 00:00:00
abstract::Using a recently developed enzyme-linked immunosorbent assay, gene dosage effects were demonstrated for the Duffy, Ss, and Rh red blood cell antigen systems. We report a comparison by an antibody-binding assay of the relative amounts of Fya and Fyb antigens on FyaFya, FyaFyb, FybFyb, FyaFyx, and FyxFyx erthrocytes, an...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1982.22683068606.x
更新日期:1982-11-01 00:00:00
abstract:BACKGROUND:During storage, the activity of the red blood cell (RBC) antioxidant system decreases. Glucose-6-phosphate dehydrogenase (G6PD) is essential for protection against oxidative stress by producing NADPH. G6PD function of RBC transfusion products is reported to remain stable during storage, but activity was meas...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13378
更新日期:2016-02-01 00:00:00
abstract:BACKGROUND:Tests for platelet-specific antibodies are important in the diagnosis of immune platelet disorders. Monoclonal antibody glycoprotein capture assays have been the gold standards in platelet antibody detection for almost 30 years. However, such assays are complex and cumbersome to perform, which limits their r...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14122
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:Evaluation of hemostasis in bleeding patients requires both accuracy and speed. STUDY DESIGN AND METHODS:As an alternative to point-of-care testing, we developed an emergency hemorrhage panel (EHP: prothrombin time [PT], fibrinogen, platelet count, hematocrit) for use in making transfusion decisions on blee...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02753.x
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND:Advances in our understanding of the risks associated with allogeneic blood transfusions (ABTs) and the growing number of patients who wish to avoid ABTs have led to the emergence of new treatment paradigms for "bloodless" medicine and surgery. STUDY DESIGN AND METHODS:Here, we review prior studies and summ...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/trf.13736
更新日期:2016-10-01 00:00:00
abstract:BACKGROUND:The RBC injury that occurs during collection of the first few milliliters of blood into the pH 5.0 ACD (NIH, Formula A) is referred to as the lesion of collection. The RBC injury was evaluated by labeling the ACD RBCs with (51)Cr and measuring the 24-hour posttransfusion survival. The effect of the acidifica...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00071.x
更新日期:2002-03-01 00:00:00
abstract:BACKGROUND:Reported hepatitis E virus (HEV) antibody assay performance characteristics are variable. Using a subset of surplus US blood donation samples, we compared assays for detecting anti-HEV immunoglobulin M (Ig)M and IgG or total anti-HEV antibodies. STUDY DESIGN AND METHODS:Samples from 5040 random blood donati...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14553
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND:Presyncopal reactions are among the most common systemic reactions experienced by blood donors, occur most frequently in novice donors, and can serve as a deterrent to future donation regardless of donation experience. This report describes the validation of a presyncopal reactions scale that can be used to ...
journal_title:Transfusion
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1537-2995.2008.01831.x
更新日期:2008-09-01 00:00:00
abstract:BACKGROUND:Travelers returning to the United States from malaria-endemic areas are at increased risk of a potentially fatal infection from Plasmodium falciparum, which requires prompt and aggressive treatment. STUDY DESIGN AND METHODS:Described is a case of a 7-year-old boy who was infected by P. falciparum while in A...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02530.x
更新日期:2010-04-01 00:00:00
abstract:BACKGROUND:Bacterial contamination or platelet (PLT) metabolism can change the pH of stored PLT concentrates (PCs). Measurement of pH for quality control is currently done on a limited basis. An easy noninvasive method was developed to obtain sequential pH measurements over time, without risking contamination and/or co...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02727.x
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND:Overproduction of beta-chemokines and genetic variations in chemokine receptors have been correlated with protection against infection by HIV-1 or slow progression to AIDS in infected individuals. STUDY DESIGN AND METHODS:The protective role of chemokines and their receptors was evaluated in a group of seve...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2000.40040461.x
更新日期:2000-04-01 00:00:00
abstract:BACKGROUND:Washing of red blood cell concentrates (RCCs) is required for potassium-sensitive transfusion recipients, including neonates in need of large-volume transfusions. When open, nonsterile washing systems are used, postwash outdate time is limited to 24 hours, often leading to problems providing the component to...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12170
更新日期:2013-08-01 00:00:00
abstract:BACKGROUND:Many transfusion services determine the titer of potentially incompatible plasma-containing products by performing a one-dilution titer at their selected titer threshold. This study compared the results of immediate spin (IS) one-dilution titers determined by three methods with a reference standard method. ...
journal_title:Transfusion
pub_type: 杂志文章,多中心研究
doi:10.1111/trf.15170
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:The Japanese Red Cross (JRC) implemented a fully automated pooling and nucleic acid amplification test (NAT) system for testing seronegative donations. The JRC sample repository and repeat blood donations allowed for lookback and follow-up studies of hepatitis B virus (HBV) DNA-positive donors, who tested ne...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2007.01234.x
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND:Deterioration in quality of platelet concentrates (PCs) during storage results from the appearance of storage lesions affecting the hemostatic functions and posttransfusion survival of platelets. These lesions depend on the preparation and pathogen inactivation methods used, duration of storage, and platelet...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.16280
更新日期:2021-02-01 00:00:00
abstract::Adenine is an approved additive to citrate-phosphate-dextrose anticoagulant for whole blood collection and extends the storage life of red blood cells. We used in vitro methods to investigate the effects of adenine on platelet function and viability during 72 hours of storage at 22 degrees C. Although the hypotonic sh...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1982.22482251208.x
更新日期:1982-07-01 00:00:00
abstract::The accumulation of the plasticizer di-2-ethylhexylphthalate (DEHP) in blood and blood components has been of considerable concern for some time. We have followed the accumulation of DEHP and one of its major metabolities, mono-2-ethylhexylphthalate (MEHP) during storage of whole blood, platelet-rich plasma, platelet ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1978.18579036383.x
更新日期:1978-09-01 00:00:00
abstract:BACKGROUND:Antigens of the human blood group system Gerbich (Ge) are located on sialoglycoproteins glycophorin C (GPC) and glycophorin D (GPD). CASE REPORT:The Ge+ proposita (RW) produced an alloanti-Ge after receiving 2 units of red cells (RBCs) during surgery. Further studies were carried out to characterize the ant...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1997.371098016440.x
更新日期:1997-10-01 00:00:00
abstract::Red blood cells stored in saline solution for two weeks at 12 C lose some of their Fya, Fyb, and Fy3 antigenicity. At the same time substances with specific inhibitory activity against appropriate Duffy antibodies appear in the saline. Similar changes could not be recognized in time-expired commercial reagent red bloo...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1981.21381201813.x
更新日期:1981-05-01 00:00:00
abstract:BACKGROUND:The formation of red blood cell (RBC) antibodies could be enhanced by the presence of inflammation caused by prolonged RBC storage, as was shown in animal studies. The low occurrence (<10%) of K-antigen in most populations often enables identification of the K+ RBC unit that triggered anti-K formation and de...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12974
更新日期:2015-06-01 00:00:00
abstract::Anti-P1 is a cold-reactive antibody often found in the serum of P2 individuals. For the past two years our reference laboratory has encouraged the use of crossmatch compatible blood for patients with Anti-P1 rather than the use of reagent-typed P1 negative units. The authors recently surveyed those hospitals that refe...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1978.18679077956.x
更新日期:1978-11-01 00:00:00
abstract:BACKGROUND:Recombinant human G-CSF is widely used to mobilize PBPCs in healthy donors for allogeneic transplantation. There have been concerns about donor safety because of splenic ruptures during G-CSF application. To address this problem, changes in splenic size in 91 healthy donors during G-CSF mobilization of allog...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2001.41020184.x
更新日期:2001-02-01 00:00:00
abstract:BACKGROUND:The RhD blood group antigen is extremely polymorphic and the DEL phenotype represents one such class of polymorphisms. The DEL phenotype prevalent in East Asian populations arises from a synonymous substitution defined as RHD*1227A. However, initially, based on genomic and cDNA studies, the genetic basis for...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14439
更新日期:2018-03-01 00:00:00
abstract::A confidential self-administered questionnaire was given to all donors prior to blood donation (n = 95,917). The questionnaire describes acquired immunodeficiency syndrome (AIDS) high-risk groups and requires the donor to designate his blood for either laboratory purposes or for transfusion. Six-hundred and twenty-sev...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1986.26687043622.x
更新日期:1986-11-01 00:00:00
abstract:BACKGROUND:Current blood transfusion standards in Canada and the United States permit transfusion of ABO-nonidentical platelets when ABO-identical platelets are not available. This practice increases the availability of platelets, a component in chronic shortage in Ontario, Canada because of the 5-day shelf-life. The i...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00037.x
更新日期:2002-02-01 00:00:00
abstract:BACKGROUND:The introduction of prestorage white cell (WBC) reduction in random-donor platelet concentrates in Canada has increased the occurrence of particulate material in PCs. The effects of filtration on platelet activation state and the activation of plasma enzyme systems were assessed. STUDY DESIGN AND METHODS:Pa...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1999.39070724.x
更新日期:1999-07-01 00:00:00
abstract::The possible beneficial role of white cells (WBCs) in donor blood has been investigated with respect to their capacity to remove bacteria. Preparations of buffy coat and whole blood, containing as well as reduced of WBCs, were inoculated with Staphylococcus epidermidis, S. aureus, Escherichia coli, Pseudomonas aerugin...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1991.31791368338.x
更新日期:1991-09-01 00:00:00
abstract::The number of cases of transfusion-associated acquired immune deficiency syndrome (TA-AIDS) that will be seen over the next few years is difficult to estimate, because of the uncertainty about the number of persons infected with the human immunodeficiency virus (HIV) via blood transfusion and about the duration of the...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1989.29890020437.x
更新日期:1989-10-01 00:00:00
abstract:BACKGROUND:The aim was to assess iron status and dietary iron intake in a sample of premenopausal female regular and new blood donors. STUDY DESIGN AND METHODS:Premenopausal women blood donors were invited to participate. Blood samples were analyzed for serum ferritin and hemoglobin. An iron checklist assessed dietary...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12347
更新日期:2014-03-01 00:00:00