Oligonucleotide-directed nucleic acid scission by micrococcal nuclease.

abstract：:A five-residue loop structure in basic fibroblast growth factor (FGF-2) which extends from amino acid residue 118 to residue 122 was replaced, by cassette mutagenesis, with the corresponding seven-residue loop structure from the structural homologue acidic fibroblast growth factor (FGF-1) or the corresponding five-res...

journal_title：Biochemistry

authors： Seddon AP,Aviezer D,Li LY,Böhlen P,Yayon A

更新日期：1995-01-24 00:00:00

abstract：:The three-dimensional structure of toxic shock syndrome toxin 1 (TSST-1) from Staphylococcus aureus has been determined and refined to an R value of 0.226 for data between 8- and 2.5-A resolution. Overall, the structure of TSST-1 is similar to that of another superantigen, staphylococcal enterotoxin B (SEB). The key d...

journal_title：Biochemistry

authors： Prasad GS,Earhart CA,Murray DL,Novick RP,Schlievert PM,Ohlendorf DH

更新日期：1993-12-21 00:00:00

abstract：:The effect of tropomyosin alone on the binding of myosin subfragment 1 (S-1) to actin was compared to that of troponin-tropomyosin, with and without Ca2+. Over a range of ionic strength and with different ligands (adenyl-5'-yl imidodiphosphate, pyrophosphate, and adenosine 5'-diphosphate), tropomyosin confers slight c...

journal_title：Biochemistry

authors： Williams DL Jr,Greene LE

更新日期：1983-05-24 00:00:00

abstract：:To gain insight into the physiological function of phosphoinositide 3-kinase (PI 3-kinase) lipid products, this study examines the interactions of the D-3 phosphoinositides with profilin and the consequent effects on actin dynamics and phosphoinositide turnover. Profilin, a ubiquitous actin-regulating protein, plays a...

journal_title：Biochemistry

authors： Lu PJ,Shieh WR,Rhee SG,Yin HL,Chen CS

更新日期：1996-11-05 00:00:00

abstract：:p21-activated kinases (PAKs) serve as effector proteins for the GTP-binding proteins Cdc42 and Rac. They are serine/threonine kinases containing the Cdc42/Rac interactive binding (CRIB) motif. The main aim of this study was to define the minimal domain of alphaPAK required for Cdc42/Rac binding. Eight stable PAK fragm...

journal_title：Biochemistry

authors： Thompson G,Owen D,Chalk PA,Lowe PN

更新日期：1998-05-26 00:00:00

abstract：:Chemoenzymatic glycan remodeling by endoglycosidase-catalyzed deglycosylation and reglycosylation is emerging as an attractive approach for producing homogeneous glycoforms of antibodies, and the success of this approach depends on the discovery of efficient endoglycosidases and their glycosynthase mutants. We report ...

journal_title：Biochemistry

authors： Tong X,Li T,Li C,Wang LX

更新日期：2018-09-04 00:00:00

abstract：:Ethanol, in vitro, is known to perturb the molecular order of the phospholipids in biological membranes, while chronic ethanol exposure, in vivo, leads to resistance to disordering. Such changes have usually been measured by electron spin resonance, utilizing fatty acid spin probes. The use of such probes is controver...

journal_title：Biochemistry

authors： Taraschi TF,Wu A,Rubin E

更新日期：1985-12-03 00:00:00

abstract：:Inflammation and subsequent cyclooxygenase-2 (COX-2) activity has long been linked with the development of cancer, although little is known about any epigenetic effects of COX-2. A product of COX-2 activation, levuglandin (LG) quickly forms covalent bonds with nearby primary amines, such as those in lysine, which lead...

journal_title：Biochemistry

authors： Carrier EJ,Zagol-Ikapitte I,Amarnath V,Boutaud O,Oates JA

更新日期：2014-04-22 00:00:00

abstract：:A new fluorescent lipid analogue, bimanephosphatidylcholine, has been synthesized for use in lipid bilayers. This probe is well suited as an energy-transfer donor with N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylethanolamine as the acceptor. Dimyristoylphosphatidylethanolamine vesicles are prepared by sonication a...

journal_title：Biochemistry

authors： Pryor C,Bridge M,Loew LM

更新日期：1985-04-23 00:00:00

abstract：:Inhibition of the yeast F(0)F(1)-ATP synthase by the regulatory peptides IF1 and STF1 was studied using intact mitochondria and submitochondrial particles from wild-type cells or from mutants lacking one or both peptides. In intact mitochondria, endogenous IF1 only inhibited uncoupled ATP hydrolysis and endogenous STF...

journal_title：Biochemistry

authors： Venard R,Brèthes D,Giraud MF,Vaillier J,Velours J,Haraux F

更新日期：2003-06-24 00:00:00

abstract：:Genomic clones encompassing all the translated sequences, the 3' untranslated sequence, and 1 kb flanking the ATG translation initiation codon of bovine tropoelastin have been obtained and characterized by restriction enzyme analysis and extensive DNA sequencing. These analyses demonstrated that functionally distinct ...

journal_title：Biochemistry

authors： Yeh H,Anderson N,Ornstein-Goldstein N,Bashir MM,Rosenbloom JC,Abrams W,Indik Z,Yoon K,Parks W,Mecham R

更新日期：1989-03-21 00:00:00

abstract：:The importance of a specific hydrogen bond between thermolysin and a phosphonamidate inhibitor, Z-NHCH2-PO(O-)-Leu-Leu (1) [Bartlett, P. A., & Marlowe, C. K. (1987) Science (Washington D.C.) 235, 569-571], has been reevaluated. We have determined the inhibition constants (binding free energies) for thermolysin of phos...

journal_title：Biochemistry

authors： Grobelny D,Goli UB,Galardy RE

更新日期：1989-06-13 00:00:00

abstract：:In an effort to gain greater insight into the molecular mechanism of the electron-transfer reactions of cytochrome b(5), the bovine cytochrome b(5)-horse cytochrome c complex has been investigated by high-resolution multidimensional NMR spectroscopy using (13)C, (15)N-labeled cytochrome b(5) expressed from a synthetic...

journal_title：Biochemistry

authors： Hom K,Ma QF,Wolfe G,Zhang H,Storch EM,Daggett V,Basus VJ,Waskell L

更新日期：2000-11-21 00:00:00

abstract：:To establish the state of protonation of quinonoid species formed nonenzymically from pyridoxal phosphate (PLP) and diethyl aminomalonate, we have studied absorption spectra of the rapidly established steady-state mixture of species. We have evaluated the formation constant and the spectrum of the mixture of Schiff ba...

journal_title：Biochemistry

authors： Metzler CM,Harris AG,Metzler DE

更新日期：1988-06-28 00:00:00

abstract：:Human lung tryptase (HLT), a trypsin-like serine proteinase stored as an active enzyme in association with heparin in mast cell granules, is released into the extracellular environment when mast cells are activated. Tryptases are unusual in that they form tetramers and bind heparin. As there are no known endogenous tr...

journal_title：Biochemistry

authors： Addington AK,Johnson DA

更新日期：1996-10-22 00:00:00

abstract：:Two series of experiments were carried out to characterize (a) peptide fragments of sarcoplasmic reticulum (SR) ATPase, based on proteolysis with different enzymes and distribution of known labels, and (b) specific labeling and functional inactivation patterns, following ATPase derivatization with dicyclohexylcarbodii...

journal_title：Biochemistry

authors： de Ancos JG,Inesi G

更新日期：1988-03-08 00:00:00

abstract：:To probe the details of protein heme interactions, we have developed a Raman difference spectroscopic technique, which allows reliable detection of very small, approximately equal to 0.01 cm-1, frequency differences. When this technique is applied to heme proteins, structural differences in the protein which perturb t...

journal_title：Biochemistry

authors： Shelnutt JA,Rousseau DL,Dethmers JK,Margoliash E

更新日期：1981-10-27 00:00:00

abstract：:Ketosteroid isomerase (KSI) is one of the most proficient enzymes catalyzing an allylic isomerization reaction at a diffusion-controlled rate. In this study of KSI, we have detailed the structures of its active site, the role of various catalytic residues, and have explained the origin of the its fast reactivity by ca...

journal_title：Biochemistry

authors： Oh KS,Cha SS,Kim DH,Cho HS,Ha NC,Choi G,Lee JY,Tarakeshwar P,Son HS,Choi KY,Oh BH,Kim KS

更新日期：2000-11-14 00:00:00

abstract：:The essential active site Fe3+ of protocatechuate 3,4-dioxygenase [3, 4-PCD, subunit structure (alphabetaFe3+)12] is bound by axial ligands, Tyr447 (147beta) and His462 (162beta), and equatorial ligands, Tyr408 (108beta), His460 (160beta), and a solvent OH- (Wat827). Recent X-ray crystallographic studies have shown th...

journal_title：Biochemistry

authors： Frazee RW,Orville AM,Dolbeare KB,Yu H,Ohlendorf DH,Lipscomb JD

更新日期：1998-02-24 00:00:00

abstract：:Reactive metabolites of environmental chemicals and drugs can cause site specific damage to the p53 tumor suppressor gene in a major pathway for genotoxicity. We report here a high-throughput, cell-free, 96-well plate magnetic bead-enzyme system interfaced with LC-MS/MS sequencing for bioactivating test chemicals and ...

journal_title：Biochemistry

authors： Malla S,Kadimisetty K,Jiang D,Choudhary D,Rusling JF

更新日期：2018-07-03 00:00:00

abstract：:The thermotropic phase behavior of 10 mixed-chain phosphatidylcholines, in excess water, has been examined and compared with that of identical-chain C(16):C(16)PC by using high-resolution differential scanning calorimetry (DSC). The molecular weights (MW) of these 11 molecular species are the same, but their delta C/C...

journal_title：Biochemistry

authors： Bultmann T,Lin HN,Wang ZQ,Huang CH

更新日期：1991-07-23 00:00:00

abstract：:A new method is described for determining molecular structures from NMR data. The approach utilizes 2D NOESY back-calculations to generate simulated spectra for structures obtained from distance geometry (DG) computations. Comparison of experimental and back-calculated spectra, including analysis of cross-peak buildup...

journal_title：Biochemistry

authors： Summers MF,South TL,Kim B,Hare DR

更新日期：1990-01-16 00:00:00

abstract：:Rabbit muscle lactate dehydrogenase (LDH) was coupled to Sepharose in such a way that each molecule is expected to be attached via only one subunit. Dissociation of the bound active enzyme by several methods all yielded immobilized subunit derivatives which were inactive. These derivatives were capable of regenerating...

journal_title：Biochemistry

authors： Chan WW,Mosbach K

更新日期：1976-09-21 00:00:00

abstract：:One of the most promising approaches to anti-hepatitis C virus drug discovery is the development of inhibitors of the virally encoded protease NS3. This chymotrypsin-like serine protease is essential for the maturation of the viral polyprotein, and processing requires complex formation between NS3 and its cofactor NS4...

journal_title：Biochemistry

authors： Bianchi E,Orrù S,Dal Piaz F,Ingenito R,Casbarra A,Biasiol G,Koch U,Pucci P,Pessi A

更新日期：1999-10-19 00:00:00

abstract：:Murine epidermal growth factor (EGF), a 53 amino acid protein, has been modified by enzymic digestion, site-specific chemical reactions, and recombinant DNA technology. After trypsin digestion the EGF derivatives EGF1-48 (called EGF-T) and EGF1-45 (called EGF-T2) were separated from the residual EGF and the C-terminal...

journal_title：Biochemistry

authors： Burgess AW,Lloyd CJ,Smith S,Stanley E,Walker F,Fabri L,Simpson RJ,Nice EC

更新日期：1988-07-12 00:00:00

abstract：:Binding of urokinase-type plasminogen activator (uPA) to its cellular receptor (uPAR) renders the cell surface a favored site for plasminogen activation. Recently, a 15-mer peptide antagonist of the uPA-uPAR interaction, with an IC50 value of 10 nM, was identified using phage display technology [Goodson, R. J., Doyle,...

journal_title：Biochemistry

authors： Ploug M,Ostergaard S,Hansen LB,Holm A,Danø K

更新日期：1998-03-17 00:00:00

abstract：:We have previously shown that both recombinant apo(a) and native Lp(a) inhibit the binding of Glu-plasminogen to fibrin surfaces [Fleury & Anglés-Cano (1991) Biochemistry 30, 7630-7638; Rouy et al. (1992) Biochemistry 31, 6332-6339]. The aim of the present study was to characterize the mechanism of this inhibition and...

journal_title：Biochemistry

authors： Hervio L,Durlach V,Girard-Globa A,Anglés-Cano E

更新日期：1995-10-17 00:00:00

abstract：:Uronate isomerase (URI) catalyzes the reversible isomerization of D-glucuronate to D-fructuronate and of D-galacturonate to D-tagaturonate. URI is a member of the amidohydrolase superfamily (AHS), a highly divergent group of enzymes that catalyze primarily hydrolytic reactions. The chemical mechanism and active site s...

journal_title：Biochemistry

authors： Nguyen TT,Fedorov AA,Williams L,Fedorov EV,Li Y,Xu C,Almo SC,Raushel FM

更新日期：2009-09-22 00:00:00

abstract：:Human S100A7 (psoriasin) is a metal-chelating protein expressed by epithelial cells. It is a 22-kDa homodimer with two EF-hand domains per subunit and two transition-metal-binding His3Asp sites at the dimer interface. Each subunit contains two cysteine residues that can exist as free thiols (S100A7red) or as an intram...

journal_title：Biochemistry

authors： Cunden LS,Brophy MB,Rodriguez GE,Flaxman HA,Nolan EM

更新日期：2017-10-31 00:00:00

abstract：:The physical origin of the extremely high thermal stability of tetraether liposomes composed of the polar lipid fraction E (PLFE) from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius has been investigated. The leakage rate of trapped 5, 6-carboxyfluorescein (5(6)CF) and the proton permeability in PLFE ...

journal_title：Biochemistry

authors： Komatsu H,Chong PL

更新日期：1998-01-06 00:00:00