Spectroscopic studies of quinonoid species from pyridoxal 5'-phosphate.

abstract：:Because oxygen and selenium are in the same group (Family VI) in the periodic table, the site-specific mutagenesis at the atomic level by replacing RNA oxygen with selenium can provide insights on the structure and function of catalytic RNAs. We report here the first Se-derivatized ribozymes transcribed with all nucle...

journal_title：Biochemistry

authors： Brandt G,Carrasco N,Huang Z

更新日期：2006-07-25 00:00:00

abstract：:Retinitis pigmentosa (RP), a neurodegenerative disorder, can arise from single point mutations in rhodopsin, leading to a cascade of protein instability, misfolding, aggregation, rod cell death, retinal degeneration, and ultimately blindness. Divalent cations, such as zinc and copper, have allosteric effects on misfol...

journal_title：Biochemistry

authors： Gleim S,Stojanovic A,Arehart E,Byington D,Hwa J

更新日期：2009-03-03 00:00:00

abstract：:The synaptotagmin (Syt) family of proteins plays an important role in vesicle docking and fusion during Ca(2+)-induced exocytosis in a wide variety of cell types. Its role as a Ca(2+) sensor derives primarily from its two C2 domains, C2A and C2B, which insert into anionic lipid membranes upon binding Ca(2+). Syt isofo...

journal_title：Biochemistry

authors： Osterberg JR,Chon NL,Boo A,Maynard FA,Lin H,Knight JD

更新日期：2015-09-22 00:00:00

abstract：:Trp151 in the lactose permease of Escherichia coli (LacY) is an important component of the sugar-binding site and the only Trp residue out of six that is in close proximity to the galactopyranoside in the structure (1PV7). The short distance between Trp151 and the sugar is favorable for Förster resonance energy transf...

journal_title：Biochemistry

authors： Smirnova IN,Kasho VN,Kaback HR

更新日期：2006-12-26 00:00:00

abstract：:The binding of Zn2+ to tubulin and the ability of this cation to promote the polymorphic assembly of the protein were examined. Equilibrium binding showed the existence of more than 60 potential Zn2+ binding sites on the dimer, including a number of high-affinity sites. The number of high-affinity sites, estimated by ...

journal_title：Biochemistry

authors： Eagle GR,Zombola RR,Himes RH

更新日期：1983-01-04 00:00:00

abstract：:(E)-4-Hydroxy-3-methylbut-2-enyl diphosphate reductase (IspH or LytB) catalyzes the terminal step of the MEP/DOXP pathway where it converts (E)-4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) into the two products, isopentenyl diphosphate and dimethylallyl diphosphate. The reaction involves the reductive elimination ...

journal_title：Biochemistry

authors： Xu W,Lees NS,Hall D,Welideniya D,Hoffman BM,Duin EC

更新日期：2012-06-19 00:00:00

abstract：:Expansions of (CTG)·(CAG) repeated DNAs are the mutagenic cause of 14 neurological diseases, likely arising through the formation and processing of slipped-strand DNAs. These transient intermediates of repeat length mutations are formed by out-of-register mispairing of repeat units on complementary strands. The three-...

journal_title：Biochemistry

authors： Slean MM,Reddy K,Wu B,Nichol Edamura K,Kekis M,Nelissen FH,Aspers RL,Tessari M,Schärer OD,Wijmenga SS,Pearson CE

更新日期：2013-02-05 00:00:00

abstract：:A strategy to design potent antagonists of human coagulation factor VIIa (FVIIa) by linking two proteins that independently inhibit activity and bind at separate, nonoverlapping sites is presented. A bifunctional inhibitor (KDTF5), comprising a Kunitz-type domain engineered to inhibit the FVIIa active site and a solub...

journal_title：Biochemistry

authors： Lee GF,Lazarus RA,Kelley RF

更新日期：1997-05-13 00:00:00

abstract：:The mechanisms of MRP1-drug binding and transport are not clear. In this study, we have characterized the interaction between MRP1 and rhodamine 123 (Rh123) using the photoreactive-iodinated analogue, [(125)I]iodoaryl azido-rhodamine 123 (or IAARh123). Photoaffinity labeling of plasma membranes from HeLa cells transfe...

journal_title：Biochemistry

authors： Daoud R,Kast C,Gros P,Georges E

更新日期：2000-12-19 00:00:00

abstract：:To probe the details of small amplitude motions in proteins, a dynamical analysis of the orientation fluctuations of two tyrosine side chains in the bovine pancreatic trypsin inhibitor is presented. Detailed results are given for the time history and correlation functions obtained for the ring motion from a molecular ...

journal_title：Biochemistry

authors： McCammon JA,Wolynes PG,Karplus M

更新日期：1979-03-20 00:00:00

abstract：:Protein phosphatase-1 (PP1) is an essential protein Ser/Thr phosphatase that is extraordinarily conserved from yeast to human, and Inhibitor-2 (I-2) is the most ancient of the heat-stable proteins specific for PP1. We identified novel I-2 homologues in Caenorhabditis elegans (Ce) and Xenopus laevis (Xe) and compared t...

journal_title：Biochemistry

authors： Li M,Satinover DL,Brautigan DL

更新日期：2007-03-06 00:00:00

abstract：:2-Cys peroxiredoxins (Prxs) are a large and diverse family of peroxidases which, in addition to their antioxidant functions, regulate cell signaling pathways, apoptosis, and differentiation. These enzymes are obligate homodimers (alpha(2)), utilizing a unique intermolecular redox-active disulfide center for the reduct...

journal_title：Biochemistry

authors： Wood ZA,Poole LB,Hantgan RR,Karplus PA

更新日期：2002-04-30 00:00:00

abstract：:In bacteria, regulatory phosphorylation of proteins at serine and/or threonine residues by Ser/Thr protein kinase (STPK) is an emerging theme in prokaryotic signaling, particularly since the prediction of the occurrence of several STPKs from genome sequencing and sequence surveys. Here we show that protein PknH posses...

journal_title：Biochemistry

authors： Molle V,Kremer L,Girard-Blanc C,Besra GS,Cozzone AJ,Prost JF

更新日期：2003-12-30 00:00:00

abstract：:The hammerhead ribozyme undergoes a well-defined two-stage conformational folding process, induced by the binding of magnesium ions. In this study, we have used isothermal titration calorimetry to analyze the thermodynamics of magnesium binding and magnesium ion-induced folding of the ribozyme. Binding to the natural ...

journal_title：Biochemistry

authors： Hammann C,Cooper A,Lilley DM

更新日期：2001-02-06 00:00:00

abstract：:The "stalk" of the prokaryotic 50S ribosomal subunit is comprised of four copies of the protein L7/L12. In Escherichia coli, L7/L12 is a dimeric protein at micromolar concentrations, which is able to undergo rapid subunit exchange. A recent structural study indicated a tetrameric arrangement of the L12 proteins isolat...

journal_title：Biochemistry

authors： Moens PD,Wahl MC,Jameson DM

更新日期：2005-03-08 00:00:00

abstract：:The early redox events involved in iron reduction and mobilization in mammalian ferritin have been investigated by several techniques. Sedimentation velocity measurements of ferritin samples with altered core sizes, prepared by partial reduction and Fe2+ chelation, suggest two different events occur during iron loss f...

journal_title：Biochemistry

authors： Jacobs DL,Watt GD,Frankel RB,Papaefthymiou GC

更新日期：1989-02-21 00:00:00

abstract：:Escherichia coli class Ib ribonucleotide reductase (RNR) converts nucleoside 5'-diphosphates to deoxynucleoside 5'-diphosphates in iron-limited and oxidative stress conditions. We have recently demonstrated in vitro that this RNR is active with both diferric-tyrosyl radical (Fe(III)(2)-Y(•)) and dimanganese(III)-Y(•) ...

journal_title：Biochemistry

authors： Cotruvo JA,Stubbe J

更新日期：2011-03-15 00:00:00

abstract：:Glutathione S-transferase, from the malarial parasite Plasmodium falciparum (PfGST), exerts a protective role in the organism and is thus considered an interesting target for antimalarial drug development. In contrast to other GSTs, it is present in solution as a tetramer and a dimer in equilibrium, which is induced b...

journal_title：Biochemistry

authors： Quesada-Soriano I,Barón C,García-Maroto F,Aguilera AM,García-Fuentes L

更新日期：2013-03-19 00:00:00

abstract：:Pantothenate kinase (PanK) catalyzes the first step of the universal five-step coenzyme A (CoA) biosynthetic pathway. The recently characterized type III PanK (PanK-III, encoded by the coaX gene) is distinct in sequence, structure and enzymatic properties from both the long-known bacterial type I PanK (PanK-I, exempli...

journal_title：Biochemistry

authors： Yang K,Strauss E,Huerta C,Zhang H

更新日期：2008-02-05 00:00:00

abstract：:The beta-glucosidase from Agrobacterium faecalis (previously Alcaligenes faecalis) has been subjected to a detailed kinetic investigation with a range of substrates to probe its specificity and mechanism. It has a relatively broad specificity for the substrate sugar moiety and exhibits a classical pH dependence for it...

journal_title：Biochemistry

authors： Kempton JB,Withers SG

更新日期：1992-10-20 00:00:00

abstract：:A likely entry/exit path for nitrogenase substrates, products, and/or protons involves residues α277(Arg), α192(Ser), and α356(Gly), all of which are highly conserved among MoFe proteins from different organisms. The α192(Ser) and α277(Arg) residues form part of a hydrogen-bonded network that also involves α195(His), ...

journal_title：Biochemistry

authors： Fisher K,Hare ND,Newton WE

更新日期：2014-10-07 00:00:00

abstract：:Many proteins utilize segmental motions to catalyze a specific reaction. The Omega loop of triosephosphate isomerase (TIM) is important for preventing the loss of the reactive enediol(ate) intermediate. The loop opens and closes even in the absence of the ligand, and the loop itself does not change conformation during...

journal_title：Biochemistry

authors： Xiang J,Jung JY,Sampson NS

更新日期：2004-09-14 00:00:00

abstract：:The symmetric, tandem GU mismatch motifs, and , which only differ in the mismatch order, have an average difference in thermodynamic stability of 2 kcal/mol at 37 degrees C. Thermodynamic studies of duplexes containing these motifs indicate the effect is largely localized to the mismatches and adjacent base pairs. The...

journal_title：Biochemistry

authors： McDowell JA,He L,Chen X,Turner DH

更新日期：1997-07-01 00:00:00

abstract：:Heparin was isolated from an unusually large human hemangioma that contained an elevated level of mast cells. Purification of multimilligram quantities of heparin from this tissue sample permitted a thorough examination of its structure and activity. Characterization of this human heparin included the following: one-d...

journal_title：Biochemistry

authors： Linhardt RJ,Ampofo SA,Fareed J,Hoppensteadt D,Mulliken JB,Folkman J

更新日期：1992-12-15 00:00:00

abstract：:Lipidic membrane systems that have been reported to be composed of sphingomyelin (SM)-cholesterol (Chol) microdomains or "rafts" by Dietrich et al. [palmitoyloleoyl-phosphatidylcholine(POPC)/SM/Chol, 1/1/1; Dietrich, C., Bagatolli, L. A., Volovyk, Z. N., Thompson, N. L., Levi, M., Jacobson, K., and Gratton, E. (2001) ...

journal_title：Biochemistry

authors： Aussenac F,Tavares M,Dufourc EJ

更新日期：2003-02-18 00:00:00

abstract：:Here we explore the use of ethidium to determine relative affinities of different gene delivery vectors for DNA and describe an improved method for studying the interaction. Specifically, we investigate the binding of poly(amidoamine) dendrimers and show that the DNA-dendrimer-ethidium system is far from thermodynamic...

journal_title：Biochemistry

authors： Fant K,Nordén B,Lincoln P

更新日期：2011-02-22 00:00:00

abstract：:Treatment of the beta 2 adrenergic receptor with the reducing agent dithiothreitol (DTT) is known to abolish ligand binding to the receptor. Interestingly, the loss of binding can be prevented by preoccupation of the receptor with ligand. It is unclear, however, whether the ligand blocks access of DTT to the receptor,...

journal_title：Biochemistry

authors： Lin S,Gether U,Kobilka BK

更新日期：1996-11-19 00:00:00

abstract：:When alpha,beta-unsaturated substrates bind to the active site of enoyl-CoA hydratase, large spectral changes can be observed [D'Ordine, R. L., et al. (1994) Biochemistry 33, 12635-12643]. The differences in the isotropic magnetic shieldings of the free and active site-bound forms of the carbonyl, alpha-, and beta-car...

journal_title：Biochemistry

authors： D'Ordine RL,Pawlak J,Bahnson BJ,Anderson VE

更新日期：2002-02-26 00:00:00

abstract：:Sterol carrier protein 2 like 2 from Aedes aegypti (AeSCP2L2) plays an important role in lipid transport in mosquitoes for its routine metabolic processes. Repeated unsuccessful attempts to crystallize ligand free SCP2L2 prompted us to undertake nuclear magnetic resonance (NMR) spectroscopy to determine its three-dime...

journal_title：Biochemistry

authors： Singarapu KK,Ahuja A,Potula PR,Ummanni R

更新日期：2016-09-06 00:00:00

abstract：:The conformational properties of purine-pyrimidine and pyrimidine-purine dinucleoside monophosphates in which the glycosidic torsion is fixed to congruent to 120 degree by the formation of a covalent link between the base and the sugar ring are explored by 1H NMR spectroscopy in order to obtain information about the s...

journal_title：Biochemistry

authors： Dhingra MM,Sarma RH,Uesugi S,Shida T,Ikehara M

更新日期：1981-08-18 00:00:00