Genetic changes of the E-cadherin and APC tumour suppressor genes in clear cell renal cell carcinoma.

Abstract:

AIMS:The roles of tumour suppressor genes: adenomatous polyposis coli (APC) and E-cadherin (CDH1) were investigated in clear cell renal cell carcinoma. METHODS:Forty-five human clear cell renal cell carcinomas were tested for APC gene instability by polymerase chain reaction/loss of heterozygosity using the restriction fragment length polymorphism method. E-cadherin gene was analysed by PCR amplification of tetranucleotide marker (D16S752) and the alleles were visualised by PAGE/silver staining. RESULTS:The overall proportion of loss of heterozygosity of the APC gene was 37.5% (9/24). D16S752 marker linked to E-cadherin gene (informativeness 91%) revealed three samples with loss of heterozygosity (7.5%). Interestingly, replication error phenotype was detected in 9.1% of clear cell renal cell carcinoma samples. Multivariate statistical analysis of samples informative for both APC and E-cadherin genes showed that, in this data set, loss of heterozygosity of the APC gene is correlated with advanced age and more severe TNM stages. Genetic changes of the E-cadherin gene, on the other hand, appear to be correlated with younger age groups and less severe TNM stages. CONCLUSIONS:Our results suggest that alterations, both in APC and E-cadherin genes, are involved in the evolution and progression of clear cell renal cell carcinoma. Microsatellite genetic instability of the E-cadherin gene indicates that another cellular mechanism, mismatch repair, may also be targeted in this malignancy.

journal_name

Pathology

journal_title

Pathology

authors

Pećina-SLaus N,Gall-TroSelj K,SLaus M,Radić K,NikuSeva-Martić T,Pavelić K

doi

10.1080/00313020410001671984

subject

Has Abstract

pub_date

2004-04-01 00:00:00

pages

145-51

issue

2

eissn

0031-3025

issn

1465-3931

pii

F6HN1LQAWT72GP86

journal_volume

36

pub_type

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