Abstract:
:In synaptosomal preparations from rat cerebral cortex, ouabain-sensitive Rb+ uptake was stimulated by ethanol (20-80 mM). Based on differential sensitivity to ouabain, 80% of this Na+,K(+)-ATPase activity represented activity of the alpha 1 isozyme while 20% was due to the alpha 2 and/or alpha 3 isozymes (alpha 2/ alpha 3). Stimulation of Na+,K(+)-ATPase was selective for the activity of alpha 2/alpha 3 which was increased by 167% in the presence of 80 mM ethanol. In this concentration range, ethanol had no effect on alpha 1 activity. Exposure of synaptosomal preparations to EGTA increased basal (no ethanol) alpha 2/alpha 3 activity with no effect on alpha 1 activity. Further, ethanol no longer stimulated alpha 2/alpha 3 activity after EGTA treatment. An EGTA extract was concentrated and desalted to yield a fraction that selectively inhibited alpha 2/alpha 3 activity when reconstituted with EGTA-treated synaptosomal preparations. This inhibition was trypsin-sensitive, suggesting protein involvement, and was prevented by 80 mM ethanol. In the presence of the inhibitory protein fraction, ethanol stimulated Na+, K(+)-ATPase activity in EGTA-treated membranes with a dose-response like that observed with the crude (no EGTA) synaptosomes. We propose that the alpha 2/alpha 3 activity of Na+,K(+)-ATPase is subject to inhibitory regulation and that ethanol stimulates this activity by releasing it from inhibition, an effect that may mimic in vivo deregulation of the enzyme by ethanol.
journal_name
Brain Resjournal_title
Brain researchauthors
Foley TD,Rhoads DEdoi
10.1016/0006-8993(94)90386-7subject
Has Abstractpub_date
1994-08-08 00:00:00pages
167-72issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(94)90386-7journal_volume
653pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::Neurofilament expression in peripheral olfactory neurons of adult rats was investigated by immunoblotting and immunohistochemistry using monoclonal antibodies specific for each of the 3 neurofilament proteins. Immunoblotting analysis of olfactory epithelium extracts demonstrated the presence of only the 200 kDa (NFH) ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)90416-s
更新日期:1991-05-31 00:00:00
abstract::Nitric oxide (NO) production in the sensory neurons of the rad nodose ganglion was studied by examining the distribuiotn of NO synthase (NOS) by use of NADPH diaphorase (NADPHD) histochemistry and immunohistochemistry ofr the presence of isoformes of NOS: neuronal (nNOS), endothelial (eNOS) and the inducible isoform (...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)03826-x
更新日期:2003-01-24 00:00:00
abstract::Human seizure disorders represent a heterogeneous collection of neuropathies, many of which are poorly understood. To investigate the etiology of seizure disorders, we have used a group of Drosophila mutants known as the bang-sensitive (BS) paralytics. The BS mutants exhibit seizure-like activity (SLA) following a wid...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.12.036
更新日期:2010-02-26 00:00:00
abstract::Intake of an unconditionally preferred taste stimulus (e.g., saccharin) is reduced by contingent administration of a drug of abuse (e.g., morphine). We examined the influence of insular cortex (IC) lesions on morphine-induced suppression of an olfactory cue and two taste stimuli with different levels of perceived inna...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.07.033
更新日期:2009-10-06 00:00:00
abstract::In this study intracellular recording techniques were used to study the synaptic events related to phase-locking of cochlear nucleus cells to low frequency stimuli. A variable degree of phase-locking was noted even with units of the same low characteristic frequency. With low frequency phase-locking units an excitator...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(76)90286-9
更新日期:1976-08-13 00:00:00
abstract::The effects of the epileptogenic agent, penicillin, on excitatory and inhibitory postsynaptic potentials (EPSPs and IPSPs) were studied in the hippocampal in vitro slice preparation. The actions of penicillin were compared to those of bicuculline, an antagonist of gamma-aminobutyric acid. Neither substance enhanced mo...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(80)90119-5
更新日期:1980-02-03 00:00:00
abstract::An HRP study of the frog's superior olivary nucleus (SON) revealed that it (1) receives reciprocal tonotopic projections from dorsal medullary nuclei and principal nuclei of the torus semicircularis (TS) bilaterally; (2) receives bilateral projections from caudalis nuclei and brainstem reticular nuclei, and unilateral...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)90998-4
更新日期:1986-01-29 00:00:00
abstract::Reverse transcription-polymerase chain reaction demonstrated the expression of heme oxygenase-1 and heme oxygenase-2 (HO-2) mRNA in the cochlea, but the former was considered to be much lower than the latter. The HO-2-like immunoreactivity was observed mainly in supporting cells in the organ of Corti and in the spiral...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)04209-9
更新日期:2003-03-14 00:00:00
abstract::Seasonally breeding animals undergo numerous physiological changes in response to changes in the length of the photoperiod. In most warm-weather breeding rodents, these changes result in reproductive quiescence during short photoperiods. It has been hypothesized that this change is mediated by changes in the activity ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2007.08.083
更新日期:2007-11-14 00:00:00
abstract::The median preoptic nucleus (MnPN) of the hypothalamus contains sleep-active neurons including sleep-active GABAergic neurons and is involved in the regulation of nonREM/REM sleep. The hypocretinergic (HCRT) neurons of the perifornical-lateral hypothalamic area (PF-LHA) and serotonergic (5-HT) neurons of the dorsal ra...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2008.07.115
更新日期:2008-10-09 00:00:00
abstract::Vascular basement membrane (BM) stabilizes brain vessels and inhibits endothelial cell cycle. Cerebral ischemia causes BM breakdown with the loss of structural BM components including collagens and laminins. In this study, the expression changes of the BM proteoglycan agrin, and the non-structural BM constituent SPARC...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.02.062
更新日期:2009-05-07 00:00:00
abstract::The retinohypothalamic tract (RHT) is a direct pathway from the retina to the suprachiasmatic nucleus (SCN). Electrical stimulation of the optic nerve or optic chiasm activates the RHT and produces shifts in phase of a circadian rhythm in SCN neuron activity in rat hypothalamic slices in vitro. The phase response curv...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91118-c
更新日期:1993-06-25 00:00:00
abstract::The effects of escapable and yoked inescapable electric tailshocks on extracellular levels of serotonin (5-HT) in the ventral hippocampus and dorsal periaqueductal gray (dPAG) were measured by in vivo microdialysis. Inescapable, but not escapable shock increased extracellular 5-HT in the ventral hippocampus relative t...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(98)00368-0
更新日期:1998-06-22 00:00:00
abstract::Local formation of oestrogens from androgens by aromatase cytochrome P-450 within brain cells is crucial for the sexual differentiation of the mammalian CNS. Aromatase activity has been detected in several brain regions of the developing rodent brain. In the present study, we used a mouse-specific, peptide-generated, ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90651-3
更新日期:1994-02-28 00:00:00
abstract::Purkinje cell dendritic arborizations were studied in HRP intracellularly stained mature neurons grown during 30-50 days in organotypic cerebellar cultures from newborn kittens. The effects of afferent fiber depletions on the final dendritic topological parameters were investigated by computer assisted methods. Three ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)90051-3
更新日期:1983-12-05 00:00:00
abstract::Incubation of rat striatal synaptosomes with the adenosine receptor agonist 2-chloroadenosine (2-CADO) produced a concentration-dependent increase of dopamine (DA) synthesis (about 50% of control value). The effect was not additive with the stimulation produced by either 10 microM forskolin or 2 mM dibutyryl cyclic AM...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)90232-6
更新日期:1988-07-26 00:00:00
abstract::Freezing and escape responses induced by gradual increases in the intensity of the electrical current applied to dorsal regions of the periaqueductal gray (dPAG) cause a distinct pattern of Fos distribution in the brain. From these studies, it has been suggested that a pathway involving the dPAG itself, dorsomedial hy...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.10.044
更新日期:2005-01-21 00:00:00
abstract::Maternal cocaine abuse has several deleterious effects in the newborn, including perinatal asphyxia, hypoxia, and hypercapnia. We hypothesized that chronic cocaine exposure during development may alter cerebral blood flow (CBF) regulation. We studied 16 neonatal rabbits that had received cocaine (20 mg/kg, i.p. b.i.d....
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00178-9
更新日期:1997-04-25 00:00:00
abstract::The cholecystokinin (CCK) concentration in individual brain stem nuclei of rat was determined using the Palkovits punch method19 and the CCK RIA3, CCK has a unique distribution in the brain stem, unlike other neuropeptides and biogenic amines8,19. In general, the CCK levels in the brain stem are 5-20% of rat cerebral ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90794-6
更新日期:1982-04-22 00:00:00
abstract::Infusion of carbachol into the lateral ventricles of rats at rates of 400 or 2000 ng/h for 6 h produced dose-related natriuresis, kaliuresis, and water drinking but no consumption of hypertonic NaCl solution. Electrolyte excretion and water intake were maximal during the first 2 h, and no further increases occurred af...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)90593-1
更新日期:1985-04-22 00:00:00
abstract::To test the hypothesis that neurons in the hypothalamic paraventricular nucleus (PVN) may be under both direct and indirect regulation by alpha melanocyte-stimulating hormone (alpha-MSH)-synthesizing neurons of the arcuate nucleus, we determined whether the retrogradely transported marker substance, cholera toxin beta...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.09.023
更新日期:2005-12-07 00:00:00
abstract::Administration of benzodiazepines at appropriate times in the circadian cycle induce phase-shifts in circadian locomotor activity. The possibility that benzodiazepine-induced shifts are mediated at the level of the suprachiasmatic nuclei (SCN), identified as the circadian pacemaker in mammals, was examined electrophys...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)90659-j
更新日期:1991-06-21 00:00:00
abstract::In human brain, regulation of agonist binding to the D2 dopamine receptors by guanine nucleotides is different between several regions. In membranes of the anterior pituitary lobe, agonist binding is fully sensitive to GTP or Gpp(NH)p, whereas it is resistant in membranes of globus pallidus. Both guanine nucleotide-se...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90345-4
更新日期:1989-04-10 00:00:00
abstract::Propofol (2,6-diisopropylphenol) is an intravenous general anaesthetic which can directly activate and positively modulate the GABAA receptor. The effects of propofol on human recombinant GABAA receptors were studied in Xenopus oocytes expressing either alpha1beta2, alpha1beta2gamma2L, or alpha2beta2gamma2L receptor i...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)01334-6
更新日期:1998-02-16 00:00:00
abstract::We have applied a technique involving multi-channel linear filtering to the problem of sorting and identifying singly unit neuronal activity in records containing several simultaneously active units. The technique is effective even when the waveforms of two or more nerve impulses are superimposed. We have applied it s...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(75)90883-5
更新日期:1975-08-22 00:00:00
abstract::Mouse neopallium was disaggregated at 3 developmental stages (E15, E18, PO) and grown in colony cultures for 7 days. On the seventh day of culturing the colonies of cells were transplanted into the cerebellums of neonatal mice. After 3 weeks the astrocytes within the transplants were identified with GFAP immunoperoxid...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(83)90096-2
更新日期:1983-01-01 00:00:00
abstract::Schwann cell is a cell type that forms myelin sheath and provides trophic supports for neuronal cells by producing neurotrophic factors such as neurotrophins and neurokines in both normal and traumatic situations. It was recently reported that after lesion of sciatic nerve, mRNA for cholinergic differentiation factor ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)01015-9
更新日期:1997-11-21 00:00:00
abstract::The results of a WGA-HRP and HRP study in the cat indicated that some neurons in the marginal zone (lamina I) of the caudal spinal trigeminal nucleus sent their axons contralaterally to the striatum; mainly to the dorsal part of the putamen, and additionally to the ventrolateral part of the caudate nucleus, at the ste...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)90400-8
更新日期:1987-04-07 00:00:00
abstract::The effects of target and non-target cells on the growth and function of intrastriatal grafts of mesencephalic dopamine neurons have been studied in rats with unilateral 6-hydroxydopamine-induced lesions of the nigrostriatal dopamine pathway. Cell suspensions of ventral mesencephalon from 14-15-day-old rat fetuses (ri...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(86)90174-4
更新日期:1986-01-01 00:00:00
abstract::Starvation inhibits egg-laying in the snail, Lymnaea stagnalis. In starved animals the neurosecretory Caudo-Dorsal Cells (CDC), which produce the egg-laying hormone, are hyperpolarized as compared to the CDC of controls. However, they are more responsive to repetitive intracellular stimulation, which induces the hormo...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90528-5
更新日期:1982-05-13 00:00:00