Abstract：
:The genetic and epigenetic effects of nutritional folate deficiency were studied in two Chinese hamster ovary (CHO) cell lines. The CHO-AA8 cell line (hemizygous at the aprt locus) and CHO-UV5 (DNA repair-deficient mutant of AA8) were cultured in Ham's F-12 medium or in custom-prepared Ham's F-12 medium lacking folic acid, thymidine, and hypoxanthine. Cells cultured acutely in the folate deficient medium exhibited initial growth arrest, followed by massive cell death and DNA fragmentation into nucleosomal multimers characteristic of apoptosis. Although prolonged culture in the folate deficient medium was cytostatic and lethal to the majority cells, minor subpopulations in both cell lines failed to initiate cell death, exhibited phenotypic abnormalities, and adapted a selective growth advantage under marginal folate conditions. These "resistant" clones exhibited major alterations in deoxynucleotide pools associated with an increase in mutant frequency at the aprt locus as detected by resistance to cytotoxicity in 8-azaadenosine. The mutation frequency in the DNA repair-deficient CHO-UV5 cells was approximately 100-fold greater than that in the parental AA8 clones, underscoring the importance of DNA repair under conditions of folate deficiency and nucleotide pool imbalance. The enhanced mutation frequency in the DNA repair-competent folate-deficient CHO-AA8 cells suggests that DNA repair activity is less effective under folate-deficient conditions. These results add to the accumulating clinical and experimental evidence relating chronic folate deficiency to genomic instability and carcinogenesis.
journal_name
Cancer Resjournal_title
Cancer researchauthors
James SJ,Basnakian AG,Miller BJsubject
Has Abstractpub_date
1994-10-01 00:00:00pages
5075-80issue
19eissn
0008-5472issn
1538-7445journal_volume
54pub_type
杂志文章相关文献
CANCER RESEARCH文献大全abstract：:Dysregulated signal transduction from receptor tyrosine kinases to phosphatidylinositol 3-kinase (PI3K), AKT (protein kinase B), and its effector FKBP-rapamycin-associated protein (FRAP) occurs via autocrine stimulation or inactivation of the tumor suppressor PTEN in many cancers. Here we demonstrate that in human pro...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2000-03-15 00:00:00
abstract：:Intracavitary instillation of radioantibodies has been proposed as therapy for anatomically confined malignant disease. To evaluate this therapeutic strategy, a monoclonal antibody reactive with human transferrin receptor (7D3) was evaluated for localization in a human malignant mesothelioma transplanted i.p. in athym...
journal_title：Cancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1990-02-01 00:00:00
abstract：:Specific progesterone binding by cultured human breast carcinoma T47D, MCF-7, and ZR75-1 cells was decreased 25-40% by epidermal growth factor (EGF), with a 50% effective dose of 0.1 nM EGF. Studies with the soluble and particulate fractions prepared after homogenization of T47D cells grown in glass roller bottles rev...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1988-09-15 00:00:00
abstract：:We used immunohistochemical procedures to study the cellular expression and distribution of cytokeratins (CKs) in rat 13762NF mammary adenocarcinoma cells growing at mammary fat pad sites and at spontaneous lymph node and lung sites. In order to establish CK distribution in normal rat mammary epithelia, immature, rest...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
abstract：:The clinical management of cutaneous melanoma would benefit significantly from a better understanding of the molecular changes that occur during melanocytic progression to a melanoma phenotype. To gain unique insights into this process, we developed a three-dimensional in vitro model that allows observations of normal...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-05-2497
更新日期：2005-11-15 00:00:00
abstract：:Hypoxia drives malignant progression in part by promoting accumulation of the oncogenic transcription factor hypoxia inducible factor-1α (HIF-1α) in tumor cells. Tumor aggressiveness also relates to elevation of the cancer stem cell-associated membrane protein CD24, which has been causally implicated in tumor formatio...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-11-3666
更新日期：2012-11-01 00:00:00
abstract：:Enhanced activation of Akt occurs in Cowden's disease, an inherited syndrome of follicular thyroid, breast, colon, and skin tumors, via inactivation of its regulatory protein, PTEN. Whereas PTEN inactivation is uncommon in sporadic thyroid cancer, activation of growth factor pathways that signal through Akt is frequen...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2001-08-15 00:00:00
abstract：:Molecular insights into how chronic stress affects lung tumorigenesis may offer new routes to chemoprevention. In this study, we show that chronic stress in mice chemically or genetically initiated for lung cancer leads to the release of norepinephrine and other catecholamines, thereby promoting lung tumorigenesis. Me...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-16-0990
更新日期：2016-11-15 00:00:00
abstract：:Previous results have suggested that methotrexate (MTX) could interfere with the repair of spontaneous DNA damage. To determine its effects on induced DNA damage, MTX was compared to hydroxyurea and arabinofuranosylcytosine (H/A), a drug combination known to block the DNA polymerase step of excision repair, for its ab...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1990-03-15 00:00:00
abstract：:This study investigated the ultrastructure and development of virus particles detected in a spontaneous mammary tumor of an 8 year old rhesus monkey. During a 3-month period of tumor growth, several biopsies were performed. Thin section electron microscopy of the tumor tissue revealed 2 types of particles: 1) an in...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1970-08-01 00:00:00
abstract：:Despite evidence that gonadotropins may facilitate peritoneal metastasis of ovarian cancer by increasing cell adhesion, the action and molecular mechanism of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in ovarian cancer invasion is not well characterized. In the present study, we investigated the e...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-05-1785
更新日期：2006-04-01 00:00:00
abstract：:Preliminary results from a pilot trial on trastuzumab's mechanism of action against operable breast tumors overexpressing Her2 suggested a role for antibody-dependent cell cytotoxicity (ADCC). To examine factors affecting ADCC intensity and variability, we extended this study to the phenotypic and functional analysis ...
journal_title：Cancer research
pub_type： 临床试验,杂志文章
doi：10.1158/0008-5472.CAN-07-2068
更新日期：2007-12-15 00:00:00
abstract：:The effect of hydrocortisone on blood-borne tumor metastasis was studied in an i.v. inoculation experiment with Ehrlich hypotetraploid clone 1, Ehrlich hypotetraploid stock, and Ehrlich hyperdiploid stock tumors. The administration of hydrocortisone before tumor inoculation resulted in increased tumor take, reduced me...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1975-04-01 00:00:00
abstract：:The discovery of Epstein-Barr virus (EBV) in 1964 gave birth to the field of viral oncology. Despite significant scientific and clinical developments in research on several other viruses discovered and linked to cancer risk much later, our understanding of EBV as a carcinogen and a possible target for therapeutic inte...
journal_title：Cancer research
pub_type： 评论,杂志文章
doi：10.1158/0008-5472.CAN-19-2996
更新日期：2019-12-01 00:00:00
abstract：:We have applied Raman spectroscopy to analyze the chemical composition of microcalcifications occurring in benign and malignant lesions in the human breast. Microcalcifications were initially separated into two categories based on their Raman spectrum: type I, calcium oxalate dihydrate, and type II, calcium hydroxyapa...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2002-09-15 00:00:00
abstract：:Human genes expressed exclusively in tumors and male germ line cells, such as those of the MAGE, BAGE, and GAGE families, encode antigens recognized by T lymphocytes, which are potentially useful for antitumor immunotherapy. To identify new genes of this type, we generated cDNA populations enriched in sequences expres...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1998-02-15 00:00:00
abstract：:Previous studies with Adriamycin-sensitive and -resistant (ADRR) MCF-7 human breast tumor cell lines indicated that Adriamycin formed significantly less hydroxyl radical (.OH) as the result of enhanced detoxification of reactive oxygen intermediates in the ADRR cell line. In order to further define the sites of drug a...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1989-07-15 00:00:00
abstract：:Activation of apoptosis is believed to be critical for the role of p53 as a tumor suppressor. Here, we report a new mouse strain carrying a human p53 transgene in the mouse p53-null background. Expression of human p53 in these mice was comparable with wild-type murine p53; however, transactivation, induction of apopto...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-05-2063
更新日期：2006-03-15 00:00:00
abstract：:The influence of methotrexate (MTX), dibutyryl cyclic AMP, and actinomycin D on production of human chorionic gonadotropin (HCG) in normal first trimester human placental organ cultures was compared. Actinomycin D (10(-8) to 10(-6) M) elevated HCG production by as much as 3.5-fold in normal placenta, and a 2-fold incr...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1987-01-15 00:00:00
abstract：:Human papillomavirus type 6a (HPV 6a) DNA was detected in a tonsillar carcinoma both as integrated and episomal molecules, and one viral-cellular junction was molecularly cloned (Bercovich et al., J. Gen Virol., 72: 2569-2572, 1991). The cellular sequence was used as a probe for the isolation of a cosmid from a normal...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:Hypoxia inducible factors (HIF) are critical mediators of the cellular response to decreased oxygen tension and are overexpressed in a number of tumors. Although HIF1alpha and HIF2alpha share a high degree of sequence homology, recent work has shown that the two alpha subunits can have contrasting and tissue-specific ...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-08-4223
更新日期：2009-04-01 00:00:00
abstract：:Increased activity, membrane association, and secretion of cathepsin B have been shown to correlate positively with invasiveness and the metastatic properties of many tumor entities. Cathepsin B is able to directly facilitate invasion by degrading extracellular matrix components or to indirectly facilitate invasion by...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:The LNCaP cell line was established from a metastatic lesion of human prostatic adenocarcinoma. The LNCaP cells grow readily in vitro (up to 8 x 10(5) cells/sq cm; doubling time, 60 hr), form clones in semisolid media, are highly resistant to human fibroblast interferon, and show an aneuploid (modal number, 76 to 91) ...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1983-04-01 00:00:00
abstract：:It has been suggested that oxygen-carrying blood substitutes, perfluorochemical (PFC) emulsions, can increase blood flow and oxygen delivery to poorly perfused tumor regions. Local cerebral blood flow was measured in male Wistar rats bearing intracranial Walker 256 tumor with and without blood-PFC exchange using [14C]...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1987-06-15 00:00:00
abstract：:Ongoing clinical trials are exploring anticancer approaches based on signaling by TRAIL, a ligand for the cell death receptors DR4 and DR5. In this study, we report on the selective apoptotic effects of multivalent DR5 binding peptides (TRAIL(mim/DR5)) on cancer cells in vitro and in vivo. Surface plasmon resonance re...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-09-2889
更新日期：2010-02-01 00:00:00
abstract：:Anticancer vaccine strategies can now target intracellular antigens that are involved in the process of malignant transformation, such as oncogene products or mutated tumor suppressor genes. Fragments of these antigens, generally 8-10 amino acids in length and complexed with MHC class I molecules, can be recognized by...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1995-04-15 00:00:00
abstract：:The Na+/I- symporter (NIS) present in the membranes of thyroid cells is responsible for the capacity of the thyroid to concentrate iodide. This allows treatment of thyroid cancers with 131I. We propose to enlarge this therapeutic strategy to nonthyroid tumors by using an adenoviral vector to deliver the NIS gene into ...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：:The rabbit uterine protein, blastokinin, is detectable as an antigen in both male and female genital tract tissues, in preimplantation embryos, and in lungs from late fetal stages, immatures, and adults. Thus, it serves as an example of how an antigen of normal tissue exists in a developmental progression from one gen...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1976-09-01 00:00:00
abstract：:The induction of epidermal ornithine decarboxylase (EC 4.1.1.17) (ODC) following topical application of the tumor-promoting agent 12-O-tetradecanoylphorbol-13-acetate (TPA) to mice can be inhibited by topical application of putrescine, the product of the enzyme. The degree of inhibition depended on both the dose and t...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1980-11-01 00:00:00
abstract：:The influence of pretreatment with piperonylbutoxide (PIP) on the biological effects of N-nitrosodiethylamine (DEN) in vivo in Syrian golden hamsters was investigated. PIP pretreatment significantly reduced covalent binding of N-[ethyl-1-14C]DEN to tissue macromolecules in trachea, lung, and liver, while it did not ch...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
