Resolution of a serum sample mix-up through the use of short tandem repeat DNA typing.

Abstract:

BACKGROUND:A sample mix-up occurred in a tissue procurement laboratory in which aliquots of serum from two tissue donors were accidentally mislabeled. The clues to the apparent mixup involved discrepant Hepatitis C test results. In an attempt to resolve the apparent mix up, DNA typing was performed using serum samples as a possible source of genomic DNA. STUDY DESIGN AND METHODS:Two hundred microliter aliquots of two reference sera and aliquots prepared from them were subjected to DNA extraction. PCR amplification of 9 STR loci was performed on the extracts and amplicons were analyzed by capillary electrophoresis. RESULTS:About 1 microg/ml of DNA was recovered from all serum samples and was of sufficient quality to direct the amplification of most, if not all STR loci allowing the mislabeled specimens to be traced to the proper tissue donor. CONCLUSIONS:Serum is a useful source of genomic DNA for STR analysis in situations in which such samples are the only source of DNA for testing. Interestingly, one of the tissue donors on life support and repeatedly receiving blood products, exhibited a mixed DNA profile indicative of the presence of DNA from multiple individuals in the bloodstream.

journal_name

Transfusion

journal_title

Transfusion

authors

Allen RW,Pritchard JK

doi

10.1111/j.0041-1132.2004.04212.x

subject

Has Abstract

pub_date

2004-12-01 00:00:00

pages

1750-4

issue

12

eissn

0041-1132

issn

1537-2995

pii

TRF04212

journal_volume

44

pub_type

杂志文章
  • Red blood cell minor antigen mismatches during chronic transfusion therapy for sickle cell anemia.

    abstract:BACKGROUND:Red blood cell (RBC) alloimmunization occurs at a high frequency in sickle cell anemia (SCA) despite serologic matching for Rh (C/c, E/e) and K antigens. RBC minor antigen genotyping allows for prediction of antigens and RH variants that may lead to alloimmunization. STUDY DESIGN AND METHODS:RBC antigen gen...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.14282

    authors: Yee MEM,Josephson CD,Winkler AM,Webb J,Luban NLC,Leong T,Stowell SR,Fasano RM

    更新日期:2017-11-01 00:00:00

  • Evaluation of a new whole-blood filter that allows preparation of platelet concentrates by platelet-rich plasma methods.

    abstract:BACKGROUND:A novel WBC-reduction in-line whole-blood (WB) filter that does not retain platelets was evaluated to assess the filtration performance and, after processing WB by the platelet-rich plasma (PRP) method, to analyze the storage quality of filtered platelet concentrate (PC) units. STUDY DESIGN AND METHODS:To a...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.0041-1132.2003.00587.x

    authors: Lozano ML,Pérez-Ceballos E,Rivera J,Paunovic D,Candela MJ,Vicente V

    更新日期:2003-12-01 00:00:00

  • Toxoplasmosis transmitted by blood transfusions.

    abstract::Blood from humans collected into heparin or citrate was inoculated with toxoplasma organisms. After storage at 4 C up to 28 days, samples were injected into the ear veins of rabbits. The test rabbits developed toxoplasmosis. Similar results were obtained by transfusing rabbits with blood obtained from rabbits subcutan...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1978.18378205142.x

    authors: Räisänen S

    更新日期:1978-05-01 00:00:00

  • The use of polymerase chain reaction in plasma pools for the concomitant detection of hepatitis C virus and HIV type 1 RNA.

    abstract:BACKGROUND:Detection of viral nucleic acids might increase blood transfusion safety through the detection of recently infected blood donors during the preseroconversion window period. Individual screening is difficult to apply, because of technical and financial constraints. STUDY DESIGN AND METHODS:A polymerase chain...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1998.381098440854.x

    authors: Yerly S,Pedrocchi M,Perrin L

    更新日期:1998-10-01 00:00:00

  • Lower alloimmunization rates in pediatric sickle cell patients on chronic erythrocytapheresis compared to chronic simple transfusions.

    abstract:BACKGROUND:Erythrocytapheresis (ECP), automated red blood cell exchange, is increasingly being used for chronic transfusion therapy in sickle cell disease (SCD) as it is an isovolumetric transfusion, is more effective in lowering hemoglobin (Hb)S, and can limit iron overload. Because ECP requires increased blood exposu...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2012.03659.x

    authors: Wahl SK,Garcia A,Hagar W,Gildengorin G,Quirolo K,Vichinsky E

    更新日期:2012-12-01 00:00:00

  • Routine bacterial screening of apheresis platelets on Day 4 using a rapid test: a 4-year single-center experience.

    abstract:BACKGROUND:The platelet (PLT) Pan Genera Detection test (PGD) is a rapid bacterial detection system used to screen PLTs for bacterial contamination. We report a single center 46-month experience with secondary screening of apheresis PLTs by PGD testing. STUDY DESIGN AND METHODS:Existing testing records of apheresis PL...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12083

    authors: Dunbar NM,Kreuter JD,Marx-Wood CR,Dumont LJ,Szczepiorkowski ZM

    更新日期:2013-10-01 00:00:00

  • Removal of prion challenge from an immune globulin preparation by use of a size-exclusion filter.

    abstract:BACKGROUND:A size-exclusion filter (Viresolve 180, Millipore Corp.) was tested for its ability to remove transmissible spongiform encephalopathies prion protein from an immune globulin preparation during ultrafiltration. STUDY DESIGN AND METHODS:Hamster-adapted 263K scrapie brain homogenate (SBH) was spiked into Rh0(D...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2002.00160.x

    authors: Van Holten RW,Autenrieth S,Boose JA,Hsieh WT,Dolan S

    更新日期:2002-08-01 00:00:00

  • Red blood cell (RBC) survival determined in humans using RBCs labeled at multiple biotin densities.

    abstract:BACKGROUND:Safe, accurate methods permitting simultaneous and/or repeated measurement of red blood cell (RBC) survival (RCS) are important to investigate pathophysiology and therapy of anemia. Methods using chromium 51 ((51) Cr)-labeled RBCs are unacceptable for infants, children, and pregnant women. We report RCS meas...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2010.02926.x

    authors: Mock DM,Matthews NI,Zhu S,Strauss RG,Schmidt RL,Nalbant D,Cress GA,Widness JA

    更新日期:2011-05-01 00:00:00

  • A prospective, randomized, double-blind controlled trial of acetaminophen and diphenhydramine pretransfusion medication versus placebo for the prevention of transfusion reactions.

    abstract:BACKGROUND:Acetaminophen and diphenhydramine are commonly used as pretransfusion medications to prevent transfusion reactions. The purpose of this study was to prospectively compare the risk of transfusion reactions in hematology/oncology patients who receive acetaminophen with diphenhydramine or placebo before transfu...

    journal_title:Transfusion

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1537-2995.2008.01858.x

    authors: Kennedy LD,Case LD,Hurd DD,Cruz JM,Pomper GJ

    更新日期:2008-11-01 00:00:00

  • Autoagglutinins with apparent anti-P specificity reactive only by low-ionic-strength salt techniques.

    abstract::Two blood samples containing autoagglutinins reactive in room temperature tests using low-ionic strength salt (LISS) reagents, but not by conventional saline-albumin or enzyme tests, are reported. The anti-P specificity of both sera, when tested near or diluted in group AB serum, was evident by their failure to agglut...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1982.22382224937.x

    authors: Judd WJ,Steiner EA,Capps RD

    更新日期:1982-05-01 00:00:00

  • A national mapping assessment of blood collection and transfusion service facilities in Afghanistan.

    abstract:BACKGROUND:The purpose of this study was to assess functionality and resources of facilities providing blood collection and transfusion services in Afghanistan. STUDY DESIGN AND METHODS:This national cross-sectional assessment included facilities collecting or transfusing blood identified through official data sources...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2012.03674.x

    authors: Mansoor GF,Rahmani AM,Kakar MA,Hashimy P,Abrahimi P,Scott PT,Peel SA,Rentas FJ,Todd CS

    更新日期:2013-01-01 00:00:00

  • A pattern of 5-1-1 and c100-3 only on hepatitis C virus (HCV) recombinant immunoblot assay does not reflect HCV infection in blood donors.

    abstract::Current criteria for a reactive (positive) interpretation on hepatitis C virus (HCV) recombinant immunoblot assay (RIBA) require > or = 1+ reactivity to at least two of the four HCV antigens present in the assay. Given that 5-1-1 is a subcomponent of c100-3, there is concern that donor samples reacting only with these...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1993.33193142316.x

    authors: Busch MP,Tobler L,Quan S,Wilber JC,Johnson P,Polito A,Steane E,Zola A,Bahl C,Nelles M

    更新日期:1993-01-01 00:00:00

  • Evaluation of the properties of components prepared and stored after holding of whole blood units for 8 and 24 hours at ambient temperature.

    abstract:BACKGROUND:The capability of holding whole blood (WB) units at ambient temperature, overnight, should help in platelet (PLT) concentrate preparation logistics. We summarize the results of a study conducted in the early 1990s that compared, in particular, PLT and red blood cell (RBC) in vivo viability properties followi...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2010.02958.x

    authors: Moroff G,AuBuchon JP,Pickard C,Whitley PH,Heaton WA,Holme S

    更新日期:2011-01-01 00:00:00

  • Further studies on the dependence of some examples of anti-M and anti-N on the presence of red-cell-borne sialic acid.

    abstract::Previous studies have shown that some examples of anti-M and anti-N fail to agglutinate neuraminidase-treated (NeuNAc-depleted) red cells. This investigation extends those observations and shows that the antibodies fail to bind to such treated red cells. These observations may mean that NeuNAc residues act to orient t...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1983.23283172846.x

    authors: Issitt PD,Wilkinson SL

    更新日期:1983-03-01 00:00:00

  • The accumulation of mono-2-ethylhexylphthalate (MEHP) during storage of whole blood and plasma.

    abstract::The accumulation of the plasticizer di-2-ethylhexylphthalate (DEHP) in blood and blood components has been of considerable concern for some time. We have followed the accumulation of DEHP and one of its major metabolities, mono-2-ethylhexylphthalate (MEHP) during storage of whole blood, platelet-rich plasma, platelet ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1978.18579036383.x

    authors: Rock G,Secours VE,Franklin CA,Chu I,Villeneuve DC

    更新日期:1978-09-01 00:00:00

  • Epidemiologic comparison of human T-lymphotropic virus type I-infected blood donors from endemic and nonendemic regions over a 3-year period.

    abstract:BACKGROUND:Screening for human T-lymphotropic virus type I (HTLV-I) infection became systematic in 1989 in the French West Indies for blood from all donors and in France for blood from natives of endemic areas; in 1990, it was extended to blood from donors with at-risk sex partners and in July 1991 to blood from all do...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1994.34394196615.x

    authors: Massari V,Elghouzzi MH,Agis F,Rannou C,Gordien E,Costagliola D,Lefrère JJ

    更新日期:1994-03-01 00:00:00

  • Therapeutic plasma exchange and intravenous immunoglobulin as primary therapy for D alloimmunization in pregnancy precludes the need for intrauterine transfusion.

    abstract:BACKGROUND:Maternal D alloimmunization detected in early gestation requires aggressive intervention to prevent severe fetal anemia. An intrauterine transfusion (IUT) is indicated to prevent fetal death once severe fetal anemia has been detected, but is not without risk. Protocols combining therapeutic plasma exchange (...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12633

    authors: Bellone M,Boctor FN

    更新日期:2014-08-01 00:00:00

  • RHCE*ceTI encodes partial c and partial e and is often in cis to RHD*DIVa.

    abstract:BACKGROUND:In the Rh blood group system, variant RhD and RhCE express several partial antigens. We investigated RH in samples with partial DIVa that demonstrated weak and variable reactivity with anti-C. STUDY DESIGN AND METHODS:Standard hemagglutination techniques, polymerase chain reaction-based assays, and RH seque...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2012.03800.x

    authors: Westhoff CM,Vege S,Halter Hipsky C,Hue-Roye K,Copeland T,Velliquette RW,Horn T,Lomas-Francis C,Reid ME

    更新日期:2013-04-01 00:00:00

  • Transfusion and component characteristics are not associated with allergic transfusion reactions to apheresis platelets.

    abstract:BACKGROUND:Transfusion-related characteristics have been hypothesized to cause allergic transfusion reactions (ATRs) but they have not been thoroughly studied. The primary objective of this study is to evaluate the associations of infusion rate, infusion volume, ABO mismatching, component age, and pretransfusion medica...

    journal_title:Transfusion

    pub_type: 临床试验,杂志文章

    doi:10.1111/trf.12852

    authors: Savage WJ,Tobian AA,Savage JH,Hamilton RG,Borge PD,Kaufman RM,Ness PM

    更新日期:2015-02-01 00:00:00

  • Determination of thromboelastographic responsiveness in stored single-donor platelet concentrates.

    abstract:BACKGROUND:Thromboelastography (TEG) is widely used in hospitals but less commonly in blood banks for evaluation of platelet (PLT) concentrates (PCs). A TEG-PC assay for testing fresh or stored PLTs must reflect the quality of the PLTs. The added value could be measurement of donor-dependent PLT quality. STUDY DESIGN ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12515

    authors: Bontekoe IJ,van der Meer PF,de Korte D

    更新日期:2014-06-01 00:00:00

  • Isolation of Kell-active protein from the red cell membrane.

    abstract::Kell blood-group-active protein has been isolated by labeling red cell surface proteins with 125I, sensitizing intact cells with anti-K1, anti-K2, anti-K7, or anti-K22, solubilizing the cell membranes, isolating immune complexes, and separating their components by sodium dodecyl sulfate polyacrylamide gel electrophore...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1984.24284173356.x

    authors: Redman CM,Marsh WL,Mueller KA,Avellino GP,Johnson CL

    更新日期:1984-03-01 00:00:00

  • Sickle Hb polymerization in RBC components from donors with sickle cell trait prevents effective WBC reduction by filtration.

    abstract:BACKGROUND:RBC components collected from donors with sickle cell trait frequently occlude WBC-reduction filters. In vitro, sickle trait RBCs have the potential for sickle Hb (Hb S) polymerization at low oxygen saturations and high Hb concentrations. STUDY DESIGN AND METHOD:To determine if the low pH and high osmolarit...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2002.00206.x

    authors: Stroncek DF,Rainer T,Sharon V,Byrne KM,Noguchi CT,Klein HG,Schechter AN,Leitman SF

    更新日期:2002-11-01 00:00:00

  • Coagulopathy after traumatic brain injury: incidence, pathogenesis, and treatment options.

    abstract::Coagulopathy after traumatic brain injury (TBI) is frequent and represents a powerful predictor related to outcome and prognosis. The complex pathophysiological mechanisms of the coagulopathy of TBI are multifactorial and remain still undefined. The nature of the coagulation abnormalities differs between severe TBI an...

    journal_title:Transfusion

    pub_type: 杂志文章,评审

    doi:10.1111/trf.12033

    authors: Maegele M

    更新日期:2013-01-01 00:00:00

  • Predonation hematocrit measurement by whole blood electrical conductivity assay.

    abstract::The whole blood electrical conductivity method for predonation hematocrit determinations was studied. Forty capillary and venous blood samples were tested concurrently with electrical conductivity and centrifugation methods. The electrical conductivity method demonstrated acceptable accuracy but was less precise than ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1985.25385219908.x

    authors: Schifman RB,Rivers SL,Delduca M

    更新日期:1985-05-01 00:00:00

  • Immune-mediated agglutination of cytoskeleton-free RBC microvesicles.

    abstract:BACKGROUND:The factors contributing to RBC agglutination are complex. The RBC cytoskeleton's participation in and contribution to this phenomenon are difficult to separate from those of the plasma membrane. Immunoreactive, cytoskeleton-free, band 3-enriched microvesicles can be generated from normal RBCs. Band 3 has be...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2000.40050566.x

    authors: Jaime JC

    更新日期:2000-05-01 00:00:00

  • Primary and secondary hemostatic functionalities of rehydrated, lyophilized platelets.

    abstract:BACKGROUND:The rehydrated, lyophilized (RL) platelet (PLT) is being developed as a hemostatic infusion agent for the control of active bleeding. The key to the method for preparing RL PLTs is a mild aldehyde stabilization that allows for freezing and lyophilizing without cellular rupture. RL PLTs have been shown to be ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2006.01002.x

    authors: Fischer TH,Bode AP,Parker BR,Russell KE,Bender DE,Ramer JK,Read MS

    更新日期:2006-11-01 00:00:00

  • Chemical analysis of a 30-year-old bottle of lyophilized plasma.

    abstract::The biochemical constituents of a 30-year-old sealed bottle of lyophilized plasma were analyzed. The results in this communication show that the process of lyophilization and storage has not seemed to cause any great changes in plasma components, including hormones, enzymes, and proteins. ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1977.17177128892.x

    authors: Fu P,Myhre B

    更新日期:1977-01-01 00:00:00

  • Passenger lymphocyte syndrome with severe hemolytic anemia due to an anti-Jk(a) after allogeneic PBPC transplantation.

    abstract:BACKGROUND:After allogeneic peripheral blood progenitor cell (PBPC) transplantation, a patient developed a severe hemolytic transfusion reaction due to passenger lymphocyte syndrome. CASE REPORT:A 50-year-old woman with secondary acute myeloid leukemia transforming from a myelodysplastic syndrome received an ABO-compa...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2000.40060632.x

    authors: Leo A,Mytilineos J,Voso MT,Weber-Nordt R,Liebisch P,Lensing C,Schraven B

    更新日期:2000-06-01 00:00:00

  • Effects of interruptions of controlled-rate freezing on the viability of umbilical cord blood stem cells.

    abstract:BACKGROUND:An optimal cooling rate is critical for cryopreserving cord blood stem cells, so it is generally achieved by using controlled-rate freezers (CRFs). The cooling rate can be interrupted by many factors during cooling; therefore, a proper response to the interruption in cryopreservation is crucial to prevent th...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12774

    authors: Yang H,Pidgorna A,Loutfy MR,Shuen P

    更新日期:2015-01-01 00:00:00

  • Peripartum hyperhemolysis prophylaxis and management in sickle cell disease: A case report and narrative review.

    abstract:BACKGROUND:Sickle cell disease (SCD) is associated with hematologic complications including delayed hemolytic transfusion reactions (DHTRs) and pregnancy-related morbidity and mortality. Hyperhemolysis syndrome (HS) is the most severe form of DHTR in patients with SCD, in which both transfused and native red blood cell...

    journal_title:Transfusion

    pub_type:

    doi:10.1111/trf.16003

    authors: Vasanthamohan L,Choo S,Marshall T,Symons YT,Matsui D,Eastabrook G,Solh Z

    更新日期:2020-08-26 00:00:00