当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Sequence effects on the conformational properties of the amyloid beta (1-28) peptide: testing a proposed mechanism for the alpha-->beta transition.

Sequence effects on the conformational properties of the amyloid beta (1-28) peptide: testing a proposed mechanism for the alpha-->beta transition.

Abstract：

:Molecular dynamics simulations have been used to successfully reproduce the observed pH-dependent conformational properties of the amyloid beta(1-28) peptide [Kirshenbaum and Daggett (1995) Biochemistry, 34, 7629-7639]. On the basis of these simulations a mechanism was proposed for the unfolding of the N-terminal portion of the peptide at neutral pH when beginning from the helical conformation. It was proposed that interactions between the side chains of Ser 8 and Glu 11 are important in determining the pH dependence of the helix content. Here we further investigate this proposed mechanism and the residues involved in the conformational transition by performing computational "mutagenesis" studies. On the basis of simulations of the mutant peptides, the importance of the Ser 8-Glu 11 interaction is substantiated, and further details of the conformational transition are elucidated.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Kirshenbaum K,Daggett V

doi

10.1021/bi00023a010

subject

Has Abstract

pub_date

1995-06-13 00:00:00

pages

7640-7

issue

23

eissn

0006-2960

issn

1520-4995

journal_volume

34

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Interactions of wild-type and mutant M protein of vesicular stomatitis virus with viral nucleocapsid and envelope in intact virions. Evidence from [125I]iodonaphthyl azide labeling and specific cross-linking.

    abstract：:Four different temperature-sensitive M protein mutants (tsM) of vesicular stomatitis virus (VSV) were characterized with regard to the association of the mutated M protein either with nucleocapsids or with membranes in the intact virions. Virions were labeled with the photoreactive hydrophobic probe [125I]iodonaphthyl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00527a020

    authors： Mancarella DA,Lenard J

    更新日期：1981-11-24 00:00:00

  • Factor IX activation by factor XIa proceeds without release of a free intermediate.

    abstract：:Factor IX activation by factor XIa is thought to proceed through the singly-cleaved free intermediate, factor IX alpha. However, we observed no intermediate development during factor IX activation by factor XIa when using a low substrate to enzyme ratio (44:1 mol/mol). This result can be explained by one of two mechan...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962274y

    authors： Wolberg AS,Morris DP,Stafford DW

    更新日期：1997-04-08 00:00:00

  • The C-terminal lipid-binding domain of apolipoprotein E is a highly efficient mediator of ABCA1-dependent cholesterol efflux that promotes the assembly of high-density lipoproteins.

    abstract：:This study was undertaken to identify the alpha-helical domains of human apoE that mediate cellular cholesterol efflux and HDL assembly via ATP-binding cassette transporter A1 (ABCA1). The C-terminal (CT) domain (residues 222-299) of apoE was found to stimulate ABCA1-dependent cholesterol efflux in a manner similar to...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi602407r

    authors： Vedhachalam C,Narayanaswami V,Neto N,Forte TM,Phillips MC,Lund-Katz S,Bielicki JK

    更新日期：2007-03-13 00:00:00

  • Differential scanning calorimetry study of local anesthetic effects on F1ATPase and submitochondrial particles.

    abstract：:The differential scanning calorimetry trace of F1ATPase, prepared from beef heart submitochondrial particles, has a single sharp endothermic transition at 80.5 +/- 1.0 degrees C and a half-height peak width of 2.0 +/- 0.2 degrees. The transition enthalpy is 19 +/- 2 cal/g of protein. Submitochondrial particles (SMP) h...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00328a022

    authors： Kresheck GC,Adade AB,Vanderkooi G

    更新日期：1985-03-26 00:00:00

  • Signal amplification between Gbetagamma release and PI3Kgamma-mediated PI(3,4,5)P3 formation monitored by a fluorescent Gbetagamma biosensor protein and repetitive two component total internal reflection/fluorescence redistribution after photobleaching an

    abstract：:Phosphoinositide 3-kinase gamma (PI3Kgamma) is activated by Gbetagamma release after stimulation of Galpha i -coupled receptors, involving a recruitment of the enzyme to the plasma membrane via interaction of the regulatory subunit p101 or p87 with Gbetagamma. The receptor-mediated release of Gbetagamma was, however, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800596b

    authors： Tannert A,Voigt P,Burgold S,Tannert S,Schaefer M

    更新日期：2008-10-28 00:00:00

  • Selective cytotoxicity of rhodium metalloinsertors in mismatch repair-deficient cells.

    abstract：:Mismatches in DNA occur naturally during replication and as a result of endogenous DNA damaging agents, but the mismatch repair (MMR) pathway acts to correct mismatches before subsequent rounds of replication. Rhodium metalloinsertors bind to DNA mismatches with high affinity and specificity and represent a promising ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi2015822

    authors： Ernst RJ,Komor AC,Barton JK

    更新日期：2011-12-20 00:00:00

  • Plasmin substrate binding site cooperativity guides the design of potent peptide aldehyde inhibitors.

    abstract：:Perioperative bleeding is a cause of major blood loss and is associated with increased rates of postoperative morbidity and mortality. To combat this, antifibrinolytic inhibitors of the serine protease plasmin are commonly used to reduce bleeding during surgery. The most effective and previously widely used of these i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201203y

    authors： Swedberg JE,Harris JM

    更新日期：2011-10-04 00:00:00

  • Molecular cloning and chromosomal assignment of the gene for human Zn-alpha 2-glycoprotein.

    abstract：:Genomic clones containing the human Zn-alpha 2-glycoprotein gene were isolated. Two of them were for the functional gene overlapped, and the other four were for two different pseudogenes (1 and 2) retaining exon-intron organization. The complete DNA sequence of the functional gene (9.3 kb) and its 5'- and 3'-flanking ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00211a004

    authors： Ueyama H,Deng HX,Ohkubo I

    更新日期：1993-12-07 00:00:00

  • Structural basis and binding properties of the second bromodomain of Brd4 with acetylated histone tails.

    abstract：:Brd4 belongs to the BET family. It is a multifunctional protein involved in transcription, replication, the signal transduction pathway, and cell cycle progression. All of these functions are linked to its association with acetylated chromatin. With its tandem bromodomains, Brd4 avidly binds to diacetylated H4-AcK5/K1...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8001659

    authors： Liu Y,Wang X,Zhang J,Huang H,Ding B,Wu J,Shi Y

    更新日期：2008-06-17 00:00:00

  • Dimeric Structure of the Blue Light Sensor Protein Photozipper in the Active State.

    abstract：:The light oxygen voltage-sensing (LOV) domain plays a crucial role in blue light (BL) sensing in plants and microorganisms. LOV domains are usually associated with the effector domains and regulate the activities of effector domains in a BL-dependent manner. Photozipper (PZ) is monomeric in the dark state. BL induces ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b01045

    authors： Ozeki K,Tsukuno H,Nagashima H,Hisatomi O,Mino H

    更新日期：2018-02-06 00:00:00

  • Cooperative binding of R17 coat protein to RNA.

    abstract：:The binding of the R17 coat protein to synthetic RNAs containing one or two coat protein binding sites was characterized by using nitrocellulose filter and gel-retention assays. RNAs with two available sites bound coat protein in a cooperative manner, resulting in a higher affinity and reduced sensitivity to pH, ionic...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00502a006

    authors： Witherell GW,Wu HN,Uhlenbeck OC

    更新日期：1990-12-18 00:00:00

  • Effect of pressure on deuterium isotope effects of yeast alcohol dehydrogenase: evidence for mechanical models of catalysis.

    abstract：:Moderate pressure accelerates hydride transfer catalyzed by yeast alcohol dehydrogenase, indicative of a large negative volume of activation [Cho and Northrop (1999) Biochemistry 38, 7470-7475]. A comparison of the effects of pressure on the oxidation of normal versus dideuteriobenzyl alcohol generates a monophasic de...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992537z

    authors： Northrop DB,Cho YK

    更新日期：2000-03-07 00:00:00

  • Osmotic swelling allows fusion of sendai virions with membranes of desialyzed erythrocytes and chromaffin granules.

    abstract：:Sendai virus particles are able to fuse with Pronase-neuraminidase-treated human erythrocyte membranes as well as with vesicles obtained from chromaffin granules of bovine medulla. Fusion is inferred either from electron microscopic studies or from the observation that incubation of fluorescently labeled (bearing octa...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00387a018

    authors： Citovsky V,Laster Y,Schuldiner S,Loyter A

    更新日期：1987-06-30 00:00:00

  • Metal-ion-dependent catalysis and specificity of CCA-adding enzymes: a comparison of two classes.

    abstract：:The CCA-adding enzymes [ATP(CTP):tRNA nucleotidyl transferases] catalyze synthesis of the conserved and essential CCA sequence to the tRNA 3' end. These enzymes are divided into two classes of distinct structures that differ in the overall orientation of the head to tail domains. However, the catalytic core of the two...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0509402

    authors： Hou YM,Gu SQ,Zhou H,Ingerman L

    更新日期：2005-09-27 00:00:00

  • Evidence for two spectroscopically different dimers of light-harvesting complex I from green plants.

    abstract：:A preparation consisting of isolated dimeric peripheral antenna complexes from green plant photosystem I (light-harvesting complex I or LHCI) has been characterized by means of (polarized) steady-state absorption and fluorescence spectroscopy at low temperatures. We show that this preparation can be described reasonab...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0007369

    authors： Ihalainen JA,Gobets B,Sznee K,Brazzoli M,Croce R,Bassi R,van Grondelle R,Korppi-Tommola JE,Dekker JP

    更新日期：2000-07-25 00:00:00

  • Hydrophobic core of the steroidogenic acute regulatory protein for cholesterol transport.

    abstract：:The steroidogenic acute regulatory protein (StAR), the first family member of START (StAR-related lipid transport) proteins, plays an essential role by facilitating the movement of cholesterol from the outer to inner mitochondrial membrane. Wild-type and mutant StAR binds cholesterol with similar intensity, but only w...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801514e

    authors： Bose HS,Whittal RM,Bose M,Debnath D

    更新日期：2009-02-17 00:00:00

  • G-1:C73 recognition by an arginine cluster in the active site of Escherichia coli histidyl-tRNA synthetase.

    abstract：:Aminoacylation of a transfer RNA (tRNA) by its cognate aminoacyl-tRNA synthetase relies upon the recognition of specific nucleotides as well as conformational features within the tRNA by the synthetase. In Escherichia coli, the aminoacylation of tRNA(His) by histidyl-tRNA synthetase (HisRS) is highly dependent upon th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035708f

    authors： Connolly SA,Rosen AE,Musier-Forsyth K,Francklyn CS

    更新日期：2004-02-03 00:00:00

  • Influence of cofactor pyridoxal 5'-phosphate on reversible high-pressure denaturation of isolated beta 2 dimer of tryptophan synthase bienzyme complex from Escherichia coli.

    abstract：:High hydrostatic pressure has been shown to cause reversible dissociation of the isolated apo beta 2 dimer of tryptophan synthase from Escherichia coli into enzymatically inactive monomers [Seifert, T., Bartholmes, P., & Jaenicke, R. (1982) Biophys. Chem. 15, 1-8]. Addition of the coenzyme pyridoxal 5'-phosphate affec...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00323a016

    authors： Seifert T,Bartholmes P,Jaenicke R

    更新日期：1985-01-15 00:00:00

  • Protein which interacts with a stimulatory factor of RNA polymerase II of Ehrlich ascites tumor cells.

    abstract：:When partially purified Ehrlich ascites tumor RNA polymerase II was further purified on a column of phosphocellulose, stimulation of its catalysis of RNA synthesis by stimulatory factor S-II was greatly decreased. This decrease in sensitivity to the stimulatory factor was reversible: the enzyme eluted from phosphocell...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00645a006

    authors： Kuroiwa A,Mizuno D,Natori S

    更新日期：1977-12-27 00:00:00

  • Mutation spectra of M13 vectors containing site-specific Cis-Syn, Trans-Syn-I, (6-4), and Dewar pyrimidone photoproducts of thymidylyl-(3'-->5')-thymidine in Escherichia coli under SOS conditions.

    abstract：:The mutations spectra of cis-syn, trans-syn-I, (6-4), and Dewar pyrimidone photoproducts of the TT site of AATTAA and TATTAT in the (-) strand of a heteroduplex M13 vector were obtained in an excision and photoreversal repair deficient Escherichia coli host under SOS conditions. Oligonucleotides containing site-specif...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi951975c

    authors： Smith CA,Wang M,Jiang N,Che L,Zhao X,Taylor JS

    更新日期：1996-04-02 00:00:00

  • New insights into inhibitor design from the crystal structure and NMR studies of Escherichia coli GAR transformylase in complex with beta-GAR and 10-formyl-5,8,10-trideazafolic acid.

    abstract：:The crystal structure of Escherichia coli GAR Tfase at 2.1 A resolution in complex with 10-formyl-5,8,10-trideazafolic acid (10-formyl-TDAF, K(i) = 260 nM), an inhibitor designed to form an enzyme-assembled multisubstrate adduct with the substrate, beta-GAR, was studied to determine the exact nature of its inhibitory ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991888a

    authors： Greasley SE,Yamashita MM,Cai H,Benkovic SJ,Boger DL,Wilson IA

    更新日期：1999-12-21 00:00:00

  • Kinetic and equilibrium alpha-secondary tritium isotope effects on reactions catalyzed by dCMP hydroxymethylase from bacteriophage T4.

    abstract：:Deoxycytidylate (dCMP) hydroxymethylase (CH) catalyzes the formation of 5-(hydroxymethyl)-dCMP, essential for DNA synthesis in phage T4, from dCMP and methylenetetrahydrofolate (CH2THF). The nucleotide analog 5-fluorodeoxuridylate (FdUMP) stoichiometrically inactivates CH by formation of a covalent complex containing ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00248a014

    authors： Graves KL,Hardy LW

    更新日期：1994-11-08 00:00:00

  • Solution structure of the second extracellular loop of human thromboxane A2 receptor.

    abstract：:Thromboxane A(2) receptor (TP receptor), a prostanoid receptor, belongs to the G protein-coupled receptor family, composed of three intracellular loops and three extracellular loops connecting seven transmembrane helices. The highly conserved extracellular domains of the prostanoid receptors were found in the second e...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001867c

    authors： Ruan KH,So SP,Wu J,Li D,Huang A,Kung J

    更新日期：2001-01-09 00:00:00

  • Tyr275 and Lys279 stabilize NADPH within the catalytic site of NADPH:protochlorophyllide oxidoreductase and are involved in the formation of the enzyme photoactive state.

    abstract：:Fluorescence spectroscopic and kinetic analysis of photochemical activity, cofactor and substrate binding, and enzyme denaturation studies were performed with highly purified, recombinant pea NADPH:protochlorophyllide oxidoreductase (POR) heterologously expressed in Escherichia coli. The results obtained with an indiv...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0105025

    authors： Lebedev N,Karginova O,McIvor W,Timko MP

    更新日期：2001-10-23 00:00:00

  • Enzyme-monitored turnover of Escherichia coli thioredoxin reductase: insights for catalysis.

    abstract：:Thioredoxin reductase from Escherichia coli is a member of the pyridine nucleotide-disulfide oxidoreductase family, and contains one FAD and one redox-active disulfide per subunit. It is known that two other well-studied members of this family, lipoamide dehydrogenase and glutathione reductase, cycle between the two e...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952521i

    authors： Lennon BW,Williams CH Jr

    更新日期：1996-04-16 00:00:00

  • Phosphatidylinositol 4,5-bisphosphate alters synaptotagmin 1 membrane docking and drives opposing bilayers closer together.

    abstract：:Synaptotagmin 1 (syt1) is a synaptic vesicle-anchored membrane protein that acts as the calcium sensor for the synchronous component of neuronal exocytosis. Using site-directed spin labeling, the position and membrane interactions of a fragment of syt1 containing its two C2 domains (syt1C2AB) were assessed in bilayers...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200049c

    authors： Kuo W,Herrick DZ,Cafiso DS

    更新日期：2011-04-05 00:00:00

  • Properties of tetrahydropteroylpentaglutamate bound to 10-formyltetrahydrofolate dehydrogenase.

    abstract：:A new rapid procedure for purifying 10-formyltetrahydrofolate dehydrogenase results in 90 mg of pure enzyme from two rabbit livers. This abundant liver enzyme is known to bind its product tetrahydropteroylpentaglutamate (H4PteGlu5) so tightly that it does not dissociate during size exclusion chromatography. 10-Formylt...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9619684

    authors： Kim DW,Huang T,Schirch D,Schirch V

    更新日期：1996-12-10 00:00:00

  • The arginine finger loop of yeast and human GAP is a determinant for the specificity toward Ras GTPase.

    abstract：:In this work, we have studied the role of the arginine finger region in determining the specificity of the GTPase activating proteins (GAPs) Saccharomyces cerevisiae Ira2p and human p120-GAP toward yeast Ras2p and human Ha-Ras p21. It is known that p120-GAP can enhance both Ras2p and Ha-Ras GTPase activities, whereas ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010027a

    authors： te Biesebeke R,Krab IM,Parmeggiani A

    更新日期：2001-06-26 00:00:00

  • On the charge regulation of proteins.

    abstract：:It is known that the overall charge of a protein can change as the molecule approaches a charged object like another protein or a cell membrane. We have formalized this mechanism using a statistical mechanical framework and show how this rather overlooked interaction increases the attraction between protein molecules....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi047630o

    authors： Lund M,Jönsson B

    更新日期：2005-04-19 00:00:00

  • Structural and functional determinants of human plasma phospholipid transfer protein activity as revealed by site-directed mutagenesis of charged amino acids.

    abstract：:Human plasma phospholipid transfer protein (PLTP) exchanges phospholipids between lipoproteins and remodels high-density lipoproteins (HDLs). We determined phospholipid transfer activity and HDL binding ability in wild-type PLTP and in 16 PLTP variants created by replacing 12 charged amino acids by site-directed mutag...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi027006g

    authors： Ponsin G,Qu SJ,Fan HZ,Pownall HJ

    更新日期：2003-04-22 00:00:00