Polymerase chain reaction in gastroenterology.

Abstract:

:Polymerase chain reaction (PCR) has had a major impact on molecular biology and is beginning to change our perception of certain disease processes and diagnostic tests. PCR allows the identification and amplification of very rare nucleic acid sequences. This is resulting in our identification of previously elusive infectious agents and of mutations that may affect the course of viral infections or carcinogenesis. PCR can now analyze the expression of genes from single cells or single molecules of nucleic acid from a sample source. Once pitfalls in contamination are overcome and more probes for genes of biological and medical interest become available, PCR technology will become universally employed in the clinical laboratory.

journal_name

J Clin Gastroenterol

authors

Hayashi PH,Zeldis JB

doi

10.1097/00004836-199306000-00014

subject

Has Abstract

pub_date

1993-06-01 00:00:00

pages

329-32

issue

4

eissn

0192-0790

issn

1539-2031

journal_volume

16

pub_type

杂志文章,评审
  • Acceptance of telemanagement is high in patients with inflammatory bowel disease.

    abstract:GOALS:Assess acceptance and attitudes regarding telemanagement (HAT) in patients with inflammatory bowel disease (IBD). BACKGROUND:Noncompliance is a barrier to successful outcomes in patients with IBD. Novel methods for monitoring and assessing compliance are needed. STUDY:HAT consists of a laptop connected to a wei...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/00004836-200603000-00006

    authors: Cross RK,Arora M,Finkelstein J

    更新日期:2006-03-01 00:00:00

  • Is smoking still important in the pathogenesis of peptic ulcer disease?

    abstract::The pathogenesis of peptic ulcer disease is multifactorial, including the effects of Helicobacter pylori, gastric acid, pepsin, gastroduodenal motility, smoking and nicotine, and the complex interaction of an array of other so-called aggressive and protective factors. Since the discovery and acceptance of H. pylori as...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章,评审

    doi:10.1097/00004836-199700001-00003

    authors: Eastwood GL

    更新日期:1997-01-01 00:00:00

  • Atypical familial presentation of FAMMM syndrome with a high incidence of pancreatic cancer: case finding of asymptomatic individuals by EUS surveillance.

    abstract:BACKGROUND:Pancreatic cancer (PC) is one of the leading causes of cancer death in Western countries. An increased risk for PC is known in a number of hereditary tumor syndromes. In selected individuals at high risk of developing PC, surveillance of the pancreas might be able to detect premalignant lesions and early inv...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/MCG.0b013e3181981123

    authors: Kluijt I,Cats A,Fockens P,Nio Y,Gouma DJ,Bruno MJ

    更新日期:2009-10-01 00:00:00

  • A survey of hepatitis C treatment clinical practice patterns using the newly approved protease inhibitors.

    abstract:GOALS:To describe current hepatitis C virus (HCV) treatment practices in the United States and identify physician characteristics associated with the use of first generation direct-acting antivirals (DAAs). BACKGROUND:HCV treatment practice patterns have not been assessed after the introduction of DAA, which are now c...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/MCG.0b013e31828a37c0

    authors: Chen EY,Lee WM,Hynan LS,Singal AG

    更新日期:2013-10-01 00:00:00

  • GERD evaluation: time for a new paradigm?

    abstract::In the past there has been reliance on heartburn and acid regurgitation as the cardinal symptoms of gastro-esophageal reflux. Now it is recognized that diverse additional symptoms including extraesophageal manifestations are essential components of gastroesophageal reflux disease (GERD). Furthermore, previous reliance...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章,评审

    doi:10.1097/01.mcg.0000225693.69745.49

    authors: Modlin IM,Malfertheiner P,Hunt RH,Armstrong D,Holtmann G,Quigley EM,Spechler SJ

    更新日期:2007-03-01 00:00:00

  • The management of postoperative chylous ascites. A case report and literature review.

    abstract::Chylous ascites remains rare as a complication of surgical intervention. Although therapeutic manoeuvres to control chylous ascites have been developed over the past 20 years, its pathophysiology is poorly understood. Conservative approaches involving dietary restriction of long-chain triglycerides and salt, together ...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章,评审

    doi:10.1097/00004836-199012000-00021

    authors: Ohri SK,Patel T,Desa LA,Spencer J

    更新日期:1990-12-01 00:00:00

  • Spontaneous umbilical fistula in Crohn's disease.

    abstract::Spontaneous umbilical fistula developed in two young women with Crohn's disease. This peculiar complication of Crohn's disease has been rarely reported. In view of the complete closure of the fistulas with medical treatment, we recommended conservative medical management, at least initially, in such patients. ...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章,评审

    doi:10.1097/00004836-198904000-00016

    authors: Veloso FT,Cardoso V,Fraga J,Carvalho J,Dias LM

    更新日期:1989-04-01 00:00:00

  • Pneumatic dilation for the treatment of achalasia in untreated patients and patients with failed Heller myotomy.

    abstract:BACKGROUND:Laparoscopic Heller myotomy (HM) has become an increasingly preferred modality to treat achalasia. However, the treatment course after a failed myotomy is controversial with fears that pneumatic dilation (PD) has high perforation risk. GOAL:To compare success and safety of graded PD with Rigiflex balloons i...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/00004836-200411000-00004

    authors: Guardino JM,Vela MF,Connor JT,Richter JE

    更新日期:2004-11-01 00:00:00

  • What does the absence of Helicobacter pylori from the antrum imply?

    abstract::Helicobacter pylori is absent in the antrum of approximately 5, 25, and 35% of patients with duodenal ulcer, gastric ulcer, and non-ulcer dyspepsia respectively and in the majority of asymptomatic healthy subjects from the West. The absence of H. pylori from the antrum could result from failure of exposure to (Group A...

    journal_title:Journal of clinical gastroenterology

    pub_type: 社论

    doi:

    authors: Desai HG,Gill HH

    更新日期:1991-10-01 00:00:00

  • Osteoporotic Fracture Risk and Health Care Burden in Patients With Cirrhosis.

    abstract:BACKGROUND AND AIMS:Risk factors for osteoporotic fracture as well as inpatient utilization and mortality have not been thoroughly studied in patients with cirrhosis. We analyzed data from a national database to determine fracture risk in patients with cirrhosis, specific risk factors, and the effect of fractures on he...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/MCG.0000000000001105

    authors: Patel A,Silverman S,Baghdadi J,Shah O,Sundaram V

    更新日期:2019-08-01 00:00:00

  • Iatrogenic achalasia. A case series.

    abstract::Between 1984 and 1992, 14 cases of "secondary" achalasia were diagnosed at our institution, five due to malignancy and nine as a result of esophageal or paraesophageal surgery. Preoperative manometry had excluded preexistent achalasia in eight of nine of the latter patients. Dysphagia developed immediately postoperati...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/00004836-199503000-00004

    authors: Ellingson TL,Kozarek RA,Gelfand MD,Botoman AV,Patterson DJ

    更新日期:1995-03-01 00:00:00

  • Colonoscope length and procedure efficiency.

    abstract::We reviewed 195 colonoscopies to determine whether procedure efficiency differed with the use of an intermediate-length (135 cm) colonoscope compared with a long (165 cm) colonoscope. The cecum was intubated with the 135-cm scope in 92.6% of procedures and with the 165-cm colonoscope in 96.8% of procedures (chi 2 meth...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/00004836-199507000-00008

    authors: Barthel J,Hinojosa T,Shah N

    更新日期:1995-07-01 00:00:00

  • Association of helicobacter pylori dupA with the failure of primary eradication.

    abstract:GOALS:To determine whether the presence of dupA Helicobacter pylori (H. pylori) influences the cure rate of primary eradication therapy. BACKGROUND:Several virulence factors of H. pylori have been reported to affect the efficacy of the eradication rate. However, no study has investigated whether the presence of dupA a...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/MCG.0b013e318243201c

    authors: Shiota S,Nguyen LT,Murakami K,Kuroda A,Mizukami K,Okimoto T,Kodama M,Fujioka T,Yamaoka Y

    更新日期:2012-04-01 00:00:00

  • Helicobacter pylori infection rate decreases in symptomatic children: a retrospective analysis of 13 years (1993-2005) from a gastroenterology clinic in West Virginia.

    abstract:BACKGROUND:The rate of Helicobacter pylori is decreasing in the developed countries, but few long-term studies are available from the United States. We retrospectively assessed the annual H. pylori infection rate in symptomatic children seen in our clinic over a 13-year study period. STUDY:A retrospective analysis of ...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/MCG.0b013e318157e4e7

    authors: Elitsur Y,Dementieva Y,Rewalt M,Lawrence Z

    更新日期:2009-02-01 00:00:00

  • Validation of a graded response questionnaire for the diagnosis of gastroesophageal reflux disease in an Asian primary care population.

    abstract:BACKGROUND:Diagnosis of gastroesophageal reflux disease (GERD) based on subjective evaluation of symptoms alone is challenging. GOALS:To test the validity and reliability of a graded response questionnaire to identify patients with GERD. STUDY:Patients presenting with upper abdominal symptoms for more than a month, a...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/MCG.0b013e3180653613

    authors: Ho KY,Gwee KA,Khor JL,Selamat DS,Yeoh KG

    更新日期:2008-07-01 00:00:00

  • Upper gastrointestinal endoscopy can be a reliable screening tool for celiac sprue in adults.

    abstract::In our study, we evaluated whether the combination of two endoscopic abnormalities in the duodenum (the "mosaic" appearance and loss of duodenal folds) is significantly associated with duodenal villous atrophy secondary to celiac sprue in adults. Fourteen patients referred for esophagogastroduodenoscopy for suspected ...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/00004836-199410000-00018

    authors: Magazzu G,Bottari M,Tuccari G,Arco A,Pallio S,Lucanto C,Tortora A,Barresi G

    更新日期:1994-10-01 00:00:00

  • Dietary habits of gallstone patients in Northern India.

    abstract::Dietary intake and plasma lipids were estimated in 200 patients with gallstones and 98 control subjects from a hospital in Northern India and were matched for age, sex, and social class. The intake of total calories and carbohydrates and the plasma triglyceride values were higher in all gallstone patients as compared ...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/00004836-199601000-00007

    authors: Tandon RK,Saraya A,Paul S,Kapur BM

    更新日期:1996-01-01 00:00:00

  • Neostigmine Administered With MoviPrep Improves Bowel Preparation for Elective Colonoscopy in Patients With Spinal Cord Injury: A Randomized Study.

    abstract:BACKGROUND:Poor preparation for elective colonoscopy is exceedingly common in persons with spinal cord injury (SCI). This unsatisfactory outcome is likely due to long-standing difficulty with evacuation and decreased colonic motility, which may result in inadequate responses to conventional bowel preparation regimens. ...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章,随机对照试验

    doi:10.1097/MCG.0000000000000284

    authors: Korsten MA,Spungen AM,Radulovic M,Rosman AS,Hunt K,Galea MD,Kornfeld SD,Lyons BL,Yen C,Bauman WA

    更新日期:2015-10-01 00:00:00

  • Risk factors for sessile serrated adenomas.

    abstract:BACKGROUND:Although sessile serrated adenomas (SSAs) may represent a separate and important pathway for colorectal cancer (CRC), little is known about the risk factors for these lesions. Molecular abnormalities such as BRAF have been observed in SSA and smokers. Our hypothesis is that smoking may be associated with the...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/MCG.0b013e318207f3cf

    authors: Anderson JC,Rangasamy P,Rustagi T,Myers M,Sanders M,Vaziri H,Wu G,Birk JW,Protiva P

    更新日期:2011-09-01 00:00:00

  • Cyclic vomiting associated with excessive dopamine in Riley-day syndrome.

    abstract:GOALS:To analyze the neurochemical profile during the recurrent attacks of nausea and vomiting in patients with Riley-day syndrome. BACKGROUND:One of the most disabling features of patients with Riley-day syndrome are recurrent attacks of severe nausea/retching/vomiting accompanied by hypertension, tachycardia, and sk...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/MCG.0b013e3182582cbf

    authors: Norcliffe-Kaufmann LJ,Axelrod FB,Kaufmann H

    更新日期:2013-02-01 00:00:00

  • Dysphagia as a primary manifestation of hyperthyroidism.

    abstract::A 69-year old woman suffered from severe dysphagia, abdominal pain, and weight loss. The dysphagia was accompanied by nasal speech, nasal regurgitation of food, weakness, and wasting of the proximal muscles of the upper and lower girdles. Laboratory data revealed T3 sephadex uptake 65.2%; T4 15.1 mcg%; and T3 250 ng%....

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/00004836-198410000-00009

    authors: Branski D,Levy J,Globus M,Aviad I,Keren A,Chowers I

    更新日期:1984-10-01 00:00:00

  • Follow-up care after a diagnosis of Helicobacter pylori infection in an Asian immigrant cohort.

    abstract:GOAL:To study the rate at which Helicobacter pylori infection is treated in an immigrant cohort after diagnosis by esophagogastroduodenoscopy (EGD). BACKGROUND:Gastric cancer is the second leading cause of cancer death worldwide, and is especially prevalent in East Asia; immigrants from this part of the world remain a...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章,多中心研究

    doi:10.1097/01.mcg.0000190755.33373.f9

    authors: Cho A,Chaudhry A,Minsky-Primus L,Tso A,Perez-Perez G,Diehl DL,Marcus SG,Gany FM

    更新日期:2006-01-01 00:00:00

  • Helicobacter pylori and gastroduodenal disorders in India--lessons from epidemiology.

    abstract::The relationship of Helicobacter pylori and gastroduodenal disorders should be compared in the developing (DG) and developed (DD) nations (Table 1) for a better understanding of the etiopathogenesis of these disorders. Although chronic gastritis is widespread in the DG nations, benign and malignant gastric ulcer (GU) ...

    journal_title:Journal of clinical gastroenterology

    pub_type: 社论

    doi:

    authors: Gill HH,Desai HG

    更新日期:1993-01-01 00:00:00

  • A screening primer: basic principles, criteria, and pitfalls of screening with comments on colorectal carcinoma.

    abstract::In this manuscript, a short review is provided of the basic principles of screening, emphasizing some of the methodologic problems attendant on any screening test, in short, a "screening primer." My analysis of the "core" data supporting efforts to screen for colorectal cancer leads to the conclusion that the utility ...

    journal_title:Journal of clinical gastroenterology

    pub_type: 临床试验,杂志文章

    doi:

    authors: Chuong JJ

    更新日期:1983-06-01 00:00:00

  • Randomized Controlled Trial: Split-dose and Same-day Large Volume Bowel Preparation for Afternoon Colonoscopy Have Similar Quality of Preparation.

    abstract:GOALS:We compare bowel cleanliness in patients taking same-day versus split-dose 4-L polyethylene glycol laxative for afternoon colonoscopy. Secondary objectives included patient satisfaction, adverse effects and patient tolerability. BACKGROUND:Afternoon colonoscopies have been associated with inadequate bowel prepar...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章,随机对照试验

    doi:10.1097/MCG.0000000000001213

    authors: Castro FJ,Al-Khairi B,Singh H,Mohameden M,Tandon K,Lopez R

    更新日期:2019-11-01 00:00:00

  • Hepatitis C virus seroconversion and genotype prevalence in patients and staff on chronic hemodialysis.

    abstract::The prevalence of hepatitis C virus (HCV)-RNA positivity among hemodialysis patients varies between 10 and 70%. Few previous surveys revealed high frequency of seroconversion of HCV-RNA-negative patients over the years of hemodialysis. Only few studies reported HCV genotype variability. We evaluated all 65 patients on...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/00004836-199901000-00005

    authors: Zamir D,Storch S,Zonder HB,Zamir C,Weiner P

    更新日期:1999-01-01 00:00:00

  • Advances in Imaging of Diffuse Parenchymal Liver Disease.

    abstract::There are >1.5 billion people with chronic liver disease worldwide, causing liver diseases to be a significant global health issue. Diffuse parenchymal liver diseases, including hepatic steatosis, fibrosis, metabolic diseases, and hepatitis cause chronic liver injury and may progress to fibrosis and eventually hepatoc...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/MCG.0000000000001380

    authors: Ghadimi M,Habibabadi RR,Hazhirkarzar B,Shaghaghi M,Ameli S,Khoshpouri P,Ghasabeh MA,Gurakar A,Pawlik TM,Kamel IR

    更新日期:2020-09-01 00:00:00

  • Helicobacter pylori infection and related gastrointestinal diseases.

    abstract::Helicobacter pylori has been implicated in the pathogenesis of a number of digestive tract disorders, such as chronic active gastritis, peptic ulceration, gastric cancer, and mucosa-associated lymphoid tissue lymphoma. Disease outcome is dependent on many factors, including bacterial genotype, host physiology and gene...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章,评审

    doi:10.1097/MCG.0b013e318030e3c3

    authors: Makola D,Peura DA,Crowe SE

    更新日期:2007-07-01 00:00:00

  • Positive predictive value of endoscopic features deemed typical of gastric fundic gland polyps.

    abstract:BACKGROUND:Fundic gland polyps (FGPs) are now the most common gastric polyp encountered by endoscopists. However, they have minimal clinical significance. If they could be reliably predicted by endoscopic features, it might prevent the need for and cost of biopsy. GOALS:To determine the positive predictive value of en...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/00004836-200305000-00007

    authors: Weston BR,Helper DJ,Rex DK

    更新日期:2003-05-01 00:00:00

  • Postpartum Laboratory Follow-up in Women With Hepatitis B in Massachusetts From 2007 to 2012.

    abstract:GOALS:To determine postpartum hepatitis B virus (HBV) laboratory testing rates and identify factors associated with a lack of follow-up testing in Massachusetts. BACKGROUND:Screening for HBV infection in pregnant women is standard of care. Guidelines recommend that patients with chronic HBV have ongoing care and labor...

    journal_title:Journal of clinical gastroenterology

    pub_type: 杂志文章

    doi:10.1097/MCG.0000000000000530

    authors: Chang MS,Barton K,Crockett M,Tuomala RE,Rutherford AE,Mutinga ML,Andersson KL,Brown RS Jr,Oken E,Ukomadu C

    更新日期:2016-07-01 00:00:00