Abstract:
:Interleukin-1 (IL-1), one of the mediators of the interaction between the immune and the neuroendocrine system, is well known to modulate anterior pituitary hormone secretion. As IL-1 influences growth of various cell types, we investigated whether IL-1 is also a growth factor of pituitary cells. We demonstrate that IL-1 dose and time dependently inhibits the growth of normal rat pituitary cells under serum-free conditions. The inhibitory potencies of IL-1 alpha (ED50, 8 pg/ml) and IL-1 beta (ED50, 6 pg/ml) were nearly identical. In the presence of low amounts of serum, both IL-1 alpha (ED50, 130 pg/ml) and IL-1 beta (ED50, 90 pg/ml) were less effective in inhibiting growth. The IL-1-induced growth inhibition was IL-1 receptor mediated, because both IL-1 alpha- and IL-1 beta-mediated growth suppression could be completely reversed by the IL-1 receptor antagonist, the physiological counterpart of IL-1. In the mammosomatotroph GH3 rat pituitary tumor cell line, IL-1 failed to influence growth, indicating that either IL-1 receptors are missing or the IL-1 signaling pathway is uncoupled from the growth regulation. In contrast to growth, in our rat pituitary monolayer cell culture system, IL-1 did not affect ACTH, GH, or PRL secretion as described previously. This discrepancy suggests the involvement of different mechanisms in the IL-1-induced mediation of growth and hormone secretion.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Renner U,Newton CJ,Pagotto U,Sauer J,Arzt E,Stalla GKdoi
10.1210/endo.136.8.7628350subject
Has Abstractpub_date
1995-08-01 00:00:00pages
3186-93issue
8eissn
0013-7227issn
1945-7170journal_volume
136pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Estrogen deficiency causes bone loss by increasing the number of bone-resorbing osteoclasts. Selective p38α MAPK inhibitors prevent bone-wasting effects of estrogen withdrawal but implicated mechanisms remain to be identified. Here, we show that inactivation of the p38α-encoding gene in osteoblast lineage cells with t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1669
更新日期:2015-12-01 00:00:00
abstract::Previous studies had suggested that norepinephrine (NE) and its precursors dopamine (DA) and L-DOPA acted similarly on iodine metabolism of isolated thyroid cells. Present studies indicate that this similarity extends to the inhibition by catecholamines of TSH-stimulated T4 release by mouse thyroids incubated in vitro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-2-632
更新日期:1986-02-01 00:00:00
abstract::As human pregnancy advances, CRH increases exponentially and is hypothesized to trigger the transition from myometrial quiescence to active contractions at labor. Paradoxically, CRH stimulates cAMP production, suggesting it should cause relaxation. To evaluate CRH as a mediator of quiescence, the effect of CRH on cont...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0348
更新日期:2009-12-01 00:00:00
abstract::Berberine, a hypoglycemic agent, has recently been shown to activate AMP-activated protein kinase (AMPK) contributing to its beneficial metabolic effects in peripheral tissues. However, whether berberine exerts a regulatory effect on beta-cells via AMPK or other signaling pathways and counteracts glucolipotoxicity rem...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1752
更新日期:2008-09-01 00:00:00
abstract::Bone remodeling is a complex process of coordinated resorption and formation of bone, which is regulated by systemic hormones and by local factors. We have previously shown that the peptide hormone adrenomedullin is mitogenic to osteoblastic cells in vitro and that it promotes bone growth in vivo. The aim of the prese...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.5.8152
更新日期:2001-05-01 00:00:00
abstract::The feedback sites of estrogen within the hypothalamus and lower brain stem involved in the suppression of pulsatile LH secretion during 48-h fasting were examined in ovariectomized rats with local estradiol (E2) implants. The animals were ovariectomized and immediately implanted stereotaxically with stainless steel c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.3.8070380
更新日期:1994-09-01 00:00:00
abstract::Vitellogenin (Vg) is a major yolk protein precursor in numerous oviparous animals. Numerous studies in bilateral oviparous animals have shown that Vg sequences are conserved across taxa and that Vgs are synthesized by somatic-cell lineages, transported to and accumulated in oocytes, and eventually used for supporting ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1086
更新日期:2013-09-01 00:00:00
abstract::Patients with Graves' disease and exophthalmos demonstrate delayed hypersensitivity to antigens present in extracts of certain normal human tissue; namely,thyroid gland and retroorbital tissue. The delayed hypersensitivity can be assayed in vitro by quantitating the amount of a lymphokine, migration inhibition factor ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-2-351
更新日期:1977-02-01 00:00:00
abstract::The effect of conditioned vs. fresh culture medium on the dopaminergic inhibition of TSH and PRL secretion by primary cultures of male rat anterior pituitary cells has been studied. In the presence of conditioned medium (that had been in contact with the cells over the 3-day culture period) 10(-6) M dopamine (DA) inhi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-4-1319
更新日期:1986-04-01 00:00:00
abstract::The effects of food restriction of the mother (65% restriction of ad libitum food intake) on fetal and maternal insulin secretion and islet function were studied at 21 days gestation in three different rat populations: 1) undernourished from 0-7 days gestation, 2) undernourished from 7-14 days gestation, and 3) undern...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.6.5202
更新日期:1997-06-01 00:00:00
abstract::Male Sprague-Dawley rats were infused continuously for 13 days with vehicle or 75 pmol (31.2 ng)/day 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] by means of Alzet osmotic minipumps implanted s.c. Animals infused with 1,25-(OH)2D3 exhibited mild hypercalcemia (11.2 vs. 10.2 mg/dl in controls), a 136% increase in the serum ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-6-2580
更新日期:1986-12-01 00:00:00
abstract::To determine if oxytocin (OT) is present in cynomolgus monkey corpus luteum, OT was measured by a specific and sensitive RIA in 13 corpora lutea, ovarian venous plasma on the ipsilateral side and peripheral venous plasma at different stages of the luteal phase. Serial dilution of acetic acid extract of the corpus lute...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-2-570
更新日期:1984-08-01 00:00:00
abstract::Rat epididymal fat cells were isolated and incubated with 25 microunits insulin/ml for 15 minutes. Insulin induced a significant shift in the saturated/unsaturated fatty acid ratio of the cerebroside fraction isolated from fat cell ghosts. This change was due to a decrease of saturated fatty acids and an increase of u...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-5-2014
更新日期:1981-05-01 00:00:00
abstract::For centuries our image of the skeleton has been one of an inert structure playing a supporting role for muscles and a protective role for inner organs like the brain. Cell biology and physiology modified this view in the 20st century by defining the constant interplay between bone-forming and bone resorbing cells tha...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa149
更新日期:2020-10-01 00:00:00
abstract::The effects of insulin on lipoprotein lipase (LPL) messenger RNA (mRNA) were studied in mammary glands from virgin and late-pregnant rats. Virgin and pregnant rats (at 17 days of gestation) were subjected to a continuous infusion (35 ml/day) with 50% glucose for 72 h to produce a prolonged hyperinsulinemic-euglycemic ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.3.6565
更新日期:1999-03-01 00:00:00
abstract::In osteoblasts, retinoic acid (RA) modulates the synthesis of various proteins, including collagen. However, little is known about the effects of RA on the regulation of interstitial collagenase synthesis and collagen degradation. After treatment of primary osteoblast-enriched (Ob) cells from fetal rat calvariae with ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.6.8194470
更新日期:1994-06-01 00:00:00
abstract::To examine the role of androgen receptor (AR) in Sertoli cells (SC), we used a SC-specific AR knockout (S-AR-/y) mouse to further evaluate the chronological changes of seminiferous tubules and the molecular mechanisms of SC androgen/AR signals on spermatogenesis. Testes morphology began changing as early as postnatal ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0138
更新日期:2006-12-01 00:00:00
abstract::Obesity is associated with systemic inflammation and elevated levels of TNFα, leading to impaired glucose tolerance. In humans, obesity is also associated with reduced nutrient-stimulated secretion of the intestinal incretin hormone, glucagon-like peptide-1 (GLP-1). We hypothesized that TNFα plays a direct role in the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1361
更新日期:2015-11-01 00:00:00
abstract::Adipokines, cytokines mainly produced by adipocytes, are active participants in the regulation of inflammation. Administration of zymosan (ZY) was used to investigate the regulation and role of adipokines during peritonitis in mice. Injection of ZY led to a significant increase in leptin levels in both serum and perit...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0327
更新日期:2008-08-01 00:00:00
abstract::The effects of FSH, hCG, and PRL on the activity of 20 alpha-hydroxysteroid dehydrogenase (20 alpha-SDH) of separate ovarian components of hypophysectomized, diethylstilbestrol-treated rats were studied. Enzyme activity was found to reside mainly in granulosa cells. FSH induced an increase in enzyme activity. A prepar...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-104-3-711
更新日期:1979-03-01 00:00:00
abstract::The distribution pattern of messenger RNAs (mRNAs) for insulin-like growth factor I (IGF-I), IGF-II, and the type 1 IGF receptor and the detection of IGF-binding sites in sections of ovine ovary were demonstrated using in situ hybridization and autoradiography. Ovaries were collected from 30 ewes at time points throug...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.12.7588270
更新日期:1995-12-01 00:00:00
abstract::The effects of cilostazol, a dual inhibitor of type 3 phosphodiesterase and adenosine uptake, on ion currents were investigated in pituitary GH(3) cells and pheochromocytoma PC12 cells. In whole-cell configuration, cilostazol (10 microm) reversibly increased the amplitude of Ca(2+)-activated K(+) current [I(K(Ca))]. C...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1430
更新日期:2004-03-01 00:00:00
abstract::The glycoprotein FSH, a product of pituitary gonadotrope cells, regulates ovarian follicle development in females and spermatogenesis in males. FSH is a heterodimer of the common α gonadotropin subunit and the hormone-specific FSHβ subunit (a product of the Fshb gene). Using a conditional knockout approach (Cre-lox), ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00100
更新日期:2018-07-01 00:00:00
abstract::In bone, early events in inflammation involve the production and release of primary proinflammatory cytokines, such as interleukin-1 beta. By activation of target cells, these cytokines are thought to induce a second wave of cytokines, including monocyte chemoattractant protein-1 (MCP-1). MCP-1 is a cytokine that stim...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.6.7750500
更新日期:1995-06-01 00:00:00
abstract::MCF-7 cells migrate through vitronectin-coated filters in response to insulin-like growth factor I (IGF-I); migration is inhibited by the matrix metalloproteinase (MMP) inhibitor BB-94, but not by the serine proteinase inhibitor aprotinin. MMP-9 was identified in the conditioned medium of MCF-7 cells; in addition, flu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.4.6623
更新日期:1999-04-01 00:00:00
abstract::Our previous work suggests that persistent protein malnutrition in immature domestic fowl (Gallus gallus domesticus) alters ACTH-adrenocortical cell interaction, possibly including ACTH receptors. To investigate this possibility, we measured some ACTH receptor parameters in isolated adrenocortical cells from normal an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-2-681
更新日期:1988-02-01 00:00:00
abstract::Prolactin (PRL) receptors are expressed in a broad range of human cell types and in a majority of human breast and prostate cancers. Experimentally, normal and malignant human cells are typically cultured in vitro in media containing bovine PRL (bPRL) from fetal bovine serum or as xenotransplants in vivo in the presen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1057
更新日期:2009-04-01 00:00:00
abstract::The effect of an i.v. administration of different doses (250, 500, and 1000 pmol) of ovine CRF (oCRF) on plasma glucose and insulin levels in lean and genetically obese fa/fa rats was investigated. In both phenotypes, i.v. CRF promoted a rapid (peak at 1 min) transient doubling of basal insulin levels without a concom...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.4.1312430
更新日期:1992-04-01 00:00:00
abstract::Mutations in Na(+)-glucose transporters (SGLT)-2 and hepatocyte nuclear factor (HNF)-1alpha genes have been related to renal glycosuria and maturity-onset diabetes of the young 3, respectively. However, the expression of these genes have not been investigated in type 1 and type 2 diabetes. Here in kidney of diabetic r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1088
更新日期:2008-02-01 00:00:00
abstract::The effects of human pancreatic GH-releasing factor-40 (hpGRF-40; 0.01-100 nM) and (Bu)2cAMP (0.015-1.5 mM) on GH release from primary monolayer cultures of pituitary cells were evaluated in rats of three age groups: postnatal days 2 and 12, and young adult males (3-4 months). Both hpGRF-40 and (Bu)2cAMP elicited a do...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-1-69
更新日期:1986-01-01 00:00:00