Abstract:
:The effects of FSH, hCG, and PRL on the activity of 20 alpha-hydroxysteroid dehydrogenase (20 alpha-SDH) of separate ovarian components of hypophysectomized, diethylstilbestrol-treated rats were studied. Enzyme activity was found to reside mainly in granulosa cells. FSH induced an increase in enzyme activity. A preparation of FSH was purified by adsorbing its LH contamination on rat corpora lutea membranes and by further neutralizing LH traces with an antiserum to the beta-subunit of LH. This purified FSH retained the ability to induce a 6-fold increase in specific enzyme activity in granulosa cells of hypophysectomized, diethylstilbestrol-treated rats. Administration of hCG to rats treated with purified FSH, further enhanced 20 alpha-SDH activity in granulosa cells up to 11.5-fold above control. PRL, which is known to inhibit 20 alpha-SDH activity in regressing rat corpora lutea, suppressed the FSH-induced increase in enzyme activity in the granulosa cells.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Eckstein B,Nimrod Adoi
10.1210/endo-104-3-711subject
Has Abstractpub_date
1979-03-01 00:00:00pages
711-4issue
3eissn
0013-7227issn
1945-7170journal_volume
104pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Rat uterine and anterior pituitary microsomes each contain a population of specific estrogen-binding sites. Saturation binding of estradiol is demonstrable, with an affinity similar to that of the cytosol estrogen receptor (Ka = 1-2 X 10(10) M-1). Dissociation rate kinetic determinations, however, revealed that estrog...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1341
更新日期:1985-10-01 00:00:00
abstract::In addition to mineralocorticoid and glucocorticoid receptors, the kidney contains a third high affinity binding site for endogenous glucocorticoids, the type III receptor. These binding sites have been localized to the collecting duct, but their biological function has not yet been identified. We have used immunodiss...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.4.8137730
更新日期:1994-04-01 00:00:00
abstract::The insulin-like growth factor I (IGF-I) receptor is a transmembrane tyrosine kinase that mediates the growth-promoting effects of IGF-I and IGF-II. Changes in IGF-I receptor messenger RNA levels are reflected in cell surface receptor number, and modulation of IGF-I receptor levels affects tumorigenicity in numerous c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.10.7065
更新日期:1999-10-01 00:00:00
abstract::The increase in glucagon-like peptide-1 (GLP-1) activity has emerged as a useful therapeutic tool for the treatment of type 2 diabetes mellitus. The actions of GLP-1 on β-cells and the nervous and digestive systems are well known. The action of this peptide in adipose tissue (AT), however, is still poorly defined. Fur...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1070
更新日期:2011-11-01 00:00:00
abstract::Prolactin (PRL) shares several characteristics with growth factors and cytokines, many of which are known to bind to heparan sulfate proteoglycans. In this study we examined the heparin-binding properties of selected members of the PRL/GH family, using heparin affinity columns followed by gel electrophoresis/Western b...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.2.6677
更新日期:1999-02-01 00:00:00
abstract::During in vitro maturation of porcine cumulus cell-oocyte complexes and in vitro luteinization of porcine granulosa cells, FSH induces the expression of the protease TNFα-converting enzyme/A disintegrin and metalloproteinase domain 17 (TACE/ADAM17) and the epidermal growth factor (EGF)-like factors, which activate the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1655
更新日期:2014-03-01 00:00:00
abstract::Transforming growth factor-beta (TGF beta) is a multifunctional growth factor in bone that is secreted as a latent complex and must be activated in order to influence cellular activity. We have investigated the influence of dexamethasone (Dex; a potent glucocorticoid) on the secretion and activation of latent TGF beta...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.5.8404670
更新日期:1993-11-01 00:00:00
abstract::TGF-β plays an important role in the progression of prostate cancer. It exhibits both tumor suppressor and tumor-promoting activities. Correlations between cyclooxygenase (COX)-2 overexpression and enhanced production of prostaglandin (PG)E2 have been implicated in cancer progression; however, there are no studies ind...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2074
更新日期:2013-05-01 00:00:00
abstract::Pineal denervation by superior cervical ganglionectomy (Gx) decreased high affinity binding of estradiol (E2) to the pineal cytosol of female rats and of testosterone to the cytosol of male rats by 40 and 26% and by 75 and 80%, 5 and 14 days after sugery; hormone binding remained unchanged up to 24 h after surgery. Bi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-4-849
更新日期:1976-04-01 00:00:00
abstract::Previous studies had suggested that norepinephrine (NE) and its precursors dopamine (DA) and L-DOPA acted similarly on iodine metabolism of isolated thyroid cells. Present studies indicate that this similarity extends to the inhibition by catecholamines of TSH-stimulated T4 release by mouse thyroids incubated in vitro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-2-632
更新日期:1986-02-01 00:00:00
abstract::Estrogens stimulate cell proliferation in typical estrogen-responsive tissues including the anterior pituitary gland. Here we report that 17-beta estradiol (E2) has estrogen receptor-mediated mitogenic and antimitogenic actions on rat lactotrophs in primary culture, depending on the cell context. E2 did not affect bas...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.7.8915
更新日期:2002-07-01 00:00:00
abstract::Cocaine- and amphetamine-regulated transcript (CART) is an anorexigenic neuropeptide synthesized in the hypothalamus. A Leu34Phe missense mutation in proCART has been found in an obese family in humans. Here we show that humans bearing the Leu34Phe mutation in proCART have severely diminished levels of bioactive CART,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0812
更新日期:2006-01-01 00:00:00
abstract::The level of fructose 2,6-bisphosphate (F2,6P2), a potent stimulator of 6-phosphofructo-1-kinase and inhibitor of fructose 1,6-bisphosphatase, was measured in three different muscle types (tensor fascia latae, biceps femoris, and soleus) and in the liver of normal and diabetic rats. The mean (+/- SEM) content of F2,6P...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-1-108
更新日期:1986-01-01 00:00:00
abstract::The effect of TSH stimulation on cellular (c)-myc mRNA content in cultured thyroid cells was examined by Northern blot analysis. Polyadenylated mRNA was prepared from quiescent FRTL-5 rat thyroid cells and from cells stimulated by TSH for 8 h. A major 2.1 kilobase (kb) transcript was enhanced 5.5-fold by TSH. (Bu)2cAM...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-5-2249
更新日期:1985-11-01 00:00:00
abstract::Intermittent PTH administration increases bone turnover, resulting in net anabolic effects on bone. These effects are primarily mediated by intracellular cAMP signaling. However, the molecular mechanisms that regulate PTH activity in bone remain incompletely understood. beta-Arrestin2, a G protein-coupled receptor reg...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1282
更新日期:2005-04-01 00:00:00
abstract::3-Iodothyronamine (3-T1AM) is an endogenous thyroid hormone (TH)-derived metabolite that induces severe hypothermia in mice after systemic administration; however, the underlying mechanisms have remained enigmatic. We show here that the rapid 3-T1AM-induced loss in body temperature is a consequence of peripheral vasod...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1951
更新日期:2017-06-01 00:00:00
abstract::The insulin receptor, following insulin stimulation of cells, triggers formation of various signaling complexes. In rat HTC hepatoma cells overexpressing normal human insulin receptors (HTC-IR), p85 regulatory subunit of phosphatidylinositol-3-kinase (PI3K) forms signaling complexes containing the insulin receptor, in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.5.6019
更新日期:1998-05-01 00:00:00
abstract::Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine produced by peripheral immune cells and also by endocrine cells in the anterior pituitary gland. MIF exerts its proinflammatory actions in the host-defense system by blocking the inhibitory effects of glucocorticoids on the release of other pro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0946
更新日期:2005-01-01 00:00:00
abstract::The Caenorhabditis elegans death susceptibility gene, ced-3, has a number of homologs in vertebrate species, including interleukin-1 beta (IL-1 beta)-converting enzyme (ICE), Ich-1long, and CPP32. These genes, which encode a family of related proteases, have been shown to induce apoptosis when transfected into eukaryo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.11.7588240
更新日期:1995-11-01 00:00:00
abstract::In rat ovary, androgen receptor (AR) is predominantly expressed in granulosa cells and is developmentally regulated. However, the exact mechanism that is responsible for the regulation of AR in granulosa cells has not been elucidated. The aim of this study was to examine 1) the levels of AR messenger RNA (mRNA) expres...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.10.8828500
更新日期:1996-10-01 00:00:00
abstract::The formation of ovarian follicles and subsequent development after birth are critical processes for female reproduction, and inappropriate coordination of these processes contributes to ovarian pathologies, such as premature ovarian failure and infertility. Identification and functional investigation of the factors i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0797
更新日期:2011-03-01 00:00:00
abstract::The nonpeptide CRH antagonist antalarmin has been shown to block both behavioral and endocrine responses to CRH. However, it's potential activity in blunting behavioral and endocrine sequelae of stressor exposure has not been assessed. Because antagonism of central CRH by alpha-helical CRH attenuates conditioned fear ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.1.6415
更新日期:1999-01-01 00:00:00
abstract::The relationship between progesterone and prostaglandin (PG) secretion in the pseudopregnant rabbit corpus luteum was investigated using isolated in vitro perfused ovaries. Progesterone and PG secretion were measured on days 1, 11, and 18 of hCG-induced pseudopregnancy. The mean progesterone secretion increased signif...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-3-1198
更新日期:1989-03-01 00:00:00
abstract::The present study used perforated-patch electrophysiology and calcium imaging in GnRH transgenic mouse lines to determine the mechanisms underlying the potent excitatory effects of kisspeptin upon GnRH neurons in the acute brain slice preparation. Kisspeptin (100 nm) depolarized (6 +/- 1 mV) and/or evoked an 87 +/- 4%...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0321
更新日期:2008-09-01 00:00:00
abstract::The initial steps involved in mediating the transduction of PTH signal via its G protein-coupled receptors are well understood and occur through the activation of cAMP and phospholipase C pathways. However, the cellular and molecular mechanisms for subsequent receptor desensitization are less well understood. Recently...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.1.7229
更新日期:2000-01-01 00:00:00
abstract::Recent evidence suggests that growth hormone-releasing peptides (GHRPs) mimic an unidentified native GH-releasing hormone (GHRH)-amplifying hormone. GHRH has been shown to stimulate food intake acting on the central nervous system. The present studies were conducted to test the hypothesis that GHRPs may also potentiat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.11.8895390
更新日期:1996-11-01 00:00:00
abstract::In an attempt to further define the optimum experimental conditions for the in vitro study of PRL endocrinology, the effect of extracellular leucine concentration on PRL secretion was assessed. Rat pituitary cells, maintained in short term monolayer culture, were washed free of serum and placed in Minimum Essential Me...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-1-80
更新日期:1984-01-01 00:00:00
abstract::We used differential display PCR on two GnRH producing cell lines to identify genes involved in GnRH gene expression and neuronal migration. RNA from Gn10 cells (derived from a tumor in the olfactory area when GnRH neurons are migrating and make low levels of GnRH) and from GT1-7 cells (derived from a tumor in forebra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.8.6221
更新日期:1998-08-01 00:00:00
abstract::Our laboratory has previously reported that physiological levels of progestins stimulate growth of the human breast cancer cell line T47D. We have also determined that the antiprogestin RU486 inhibits this stimulation. Here we present the unexpected finding that RU486 alone also stimulates growth of these breast cance...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-5-2642
更新日期:1989-05-01 00:00:00
abstract::High-fat diet and intrauterine growth retardation may predispose to obesity, insulin resistance, and type 2 diabetes. Because prenatal ethanol (ETOH) exposure causes intrauterine growth retardation, we investigated its interactions with postnatal high-fat diet on glucose tolerance and adipocyte-derived hormones in the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220623
更新日期:2003-02-01 00:00:00