Abstract：
:Transport and metabolism of adenosine were studied in the S1, S2, and S3 segments of the rabbit proximal renal tubule. Isolated segments were perfused in vitro with uniformly labelled 14C-adenosine to measure the lumen-to-bath flux of adenosine. This flux rate was measured by the disappearance of 14C from the luminal fluid (JD) and simultaneously by the appearance of 14C in the bathing solution (JA), expressed as femtomoles per minute per millimeter of tubule length (fmol.min-1.mm-1). At a perfused concentration of 83.3 microM adenosine, when corrected for metabolism, the JDs for adenosine in the S1, S2, and S3 segments were 735, 212, and 273, respectively. JAs, corrected for metabolism, were 0, 0, and 4.8 fmol.min-1.mm-1 for the S1, S2, and S3 segments, indicating that very little or no 14C-adenosine moved across the basolateral membrane. To correct for metabolism of 14C-adenosine, the perfusion fluid, collected fluid, tubular extract, and bathing fluid, from three tubules of each segment type, were analyzed by high-performance liquid chromatography to identify 14C-adenosine and its 14C-metabolites. At 83.3 microM, all segments metabolized adenosine extensively. Consequently, adenosine-5'-monophosphate (AMP) and inosine were found in tubule cells of all segments. Inosine also appeared in the collected fluid, but AMP did not. In S1 and S2 segments, none of the 14C in the bathing solutions could be identified and no adenosine was found. Of the small amounts of 14C found in bathing solutions from S3 segments, about 27% appeared to be adenosine, the rest were inosine and hypoxanthine or unidentified metabolites.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Barfuss DW,McCann WP,Katholi REdoi
10.1038/ki.1992.174subject
Has Abstractpub_date
1992-05-01 00:00:00pages
1143-9issue
5eissn
0085-2538issn
1523-1755pii
S0085-2538(15)57603-5journal_volume
41pub_type
杂志文章abstract：:IgA nephropathy (IgAN) often shows lesions morphologically identical with those of focal segmental glomerulosclerosis (FSGS). In order to determine the possible role of FSGS in IgAN lesions, we measured glomerular capsular adhesions, often the first step toward FSGS, in biopsies from 127 patients with IgAN, 100 with l...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2010.466
更新日期：2011-03-01 00:00:00
abstract：:The terminally differentiated podocyte, also called glomerular visceral epithelial cell, are highly specialized cells. They function as a critical size and charge barrier to prevent proteinuria. Podocytes are injured in diabetic and non-diabetic renal diseases. The clinical signature of podocyte injury is proteinuria,...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/sj.ki.5000410
更新日期：2006-06-01 00:00:00
abstract：BACKGROUND:Previous experiments from our laboratory have demonstrated that HKalpha(2) coimmunoprecipitated with beta(1)-Na(+),K(+)-ATPase. Although HKalpha(2) is expressed abundantly in the apical membrane of distal colon, the demonstration that beta(1) localizes to this same membrane in distal colon has not been demon...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2004.00856.x
更新日期：2004-09-01 00:00:00
abstract：:Previous work has documented an acceleration of proteolysis and branched-chain amino acid oxidation when muscles from rats with chronic metabolic acidosis were incubated in vitro. The present study examines the impact of chronic metabolic acidosis on whole body amino acid turnover and oxidation in chronically catheter...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1992.223
更新日期：1992-06-01 00:00:00
abstract：:Recent studies including the current article by Sawinski et al. demonstrate that hepatitis C virus (HCV) infection is associated with worse outcomes in kidney transplant recipients with and without HIV infection. We comment on the significance of these findings in the context of newer options for the treatment of HCV ...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2015.180
更新日期：2015-08-01 00:00:00
abstract：:Modern dietary habits are characterized by high-sodium and low-potassium intakes, each of which was correlated with a higher risk for hypertension. In this study, we examined whether long-term variations in the intake of sodium and potassium induce lasting changes in the plasma concentration of circulating steroids by...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2011.15
更新日期：2011-08-01 00:00:00
abstract：:The role of calcitonin on the calcemic response to parathyroid hormone (PTH) in renal failure has not been evaluated previously. Often animal studies evaluating the calcemic response to PTH in renal failure are performed in thyroparathyroidectomized (TPTX) animals, and thus eliminate any potential physiologic effect o...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1991.203
更新日期：1991-08-01 00:00:00
abstract：:Metabolic acidosis is common in patients with chronic kidney disease, which is known to affect bone metabolism. We examined the effect of metabolic acidosis on the development of vascular and other soft-tissue calcifications in uremic rats treated with calcitriol. Extraskeletal calcification was measured in vivo, in c...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5002646
更新日期：2008-02-01 00:00:00
abstract：:Identifying subjects with progressive chronic kidney disease will be important both in clinical practice and in conducting clinical trials. Pruijm et al. (in this issue) demonstrate for the first time that cortical oxygenation as evaluated by blood oxygenation level-dependent magnetic resonance imaging can predict fut...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2017.11.029
更新日期：2018-04-01 00:00:00
abstract：:Vascular calcification (VC) is an important reason for the high burden of vascular disease among chronic dialysis patients. Chronic kidney disease (CKD) is associated with increased promoters and decreased inhibitors of VC. The circulating levels of fetuin-A, a well-described inhibitor of calcification, regulate the c...
journal_title：Kidney international
pub_type： 评论,杂志文章,评审
doi：10.1038/sj.ki.5002355
更新日期：2007-07-01 00:00:00
abstract：:To evaluate the underlying mechanism of the putative renal protective effects of angiotensin converting enzyme (ACE) inhibitors, the modulatory action of captopril on the angiotensin II (Ang II) and platelet-derived growth factor (PDGF)-induced increase of cytosolic free calcium concentration ([Ca2+]i) was investigate...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1994.323
更新日期：1994-09-01 00:00:00
abstract：:Renal toxicity, possibly due to vasoconstriction and vascular injury, is the most relevant side-effect of chronic cyclosporine (CsA) therapy given to prevent graft rejection. In kidney transplant recipients each oral dose of CsA is invariably followed by a transient reduction in renal plasma flow (RPF) and glomerular ...
journal_title：Kidney international
pub_type： 临床试验,杂志文章
doi：10.1038/ki.1993.101
更新日期：1993-03-01 00:00:00
abstract：:We previously demonstrated that urinary endothelin excretion is increased in rats with extensive renal mass reduction, a model of progressive renal disease. Here we explored whether the increased urinary endothelin in this model were due to induction of renal pre-pro-endothelin-1 gene and whether changes in endothelin...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1993.53
更新日期：1993-02-01 00:00:00
abstract：:Leucocyte interaction with other cells is facilitated by the adhesion molecules, leucocyte function-associated antigen-1 (LFA-1)-binding to intercellular adhesion molecule-1 (ICAM-1) and for T cells and natural killer (NK) cells the binding of LFA-2 (CD2) to LFA-3. As these interactions are critical for the mediation ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1989.303
更新日期：1989-12-01 00:00:00
abstract：:Gain-of-function variants in CFB encoding factor B (FB), a component of the alternative pathway C3 convertase, have been reported in a minority of patients with aHUS and result in massive C3 activation. Zhang et al. describe the functional characterization of a novel FB variant (p.Ser367Arg) that they identified in 2 ...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2020.06.026
更新日期：2020-11-01 00:00:00
abstract：:Urinary peptidomics focuses on endogenous urinary peptide content. Many studies now show the usefulness of this approach for the discovery and validation of biomarkers in kidney diseases that are as varied as chronic kidney disease, acute kidney injury, congenital anomalies of the kidney and the urinary tract, and pol...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1016/j.kint.2015.10.010
更新日期：2016-03-01 00:00:00
abstract：BACKGROUND:Aquaporin-2 (AQP-2) proteins are mainly expressed at the apical region of the collecting duct cells. We previously reported three different mutations in the C-terminus of AQP-2 that all-cause autosomal-dominant nephrogenic diabetes insipidus. When one of these mutant AQP-2s was expressed in Madin-Darby canin...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2005.00654.x
更新日期：2005-11-01 00:00:00
abstract：:Despite the importance of cardiovascular disease in dialysis patients, the frequency of atrial fibrillation in incident dialysis patients has not been determined. We analyzed the prevalence of atrial fibrillation in patients starting dialysis over a 4-year period, its occurrence over the course of dialysis, and its in...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2009.185
更新日期：2009-08-01 00:00:00
abstract：:The Standardized Outcomes in Nephrology (SONG) initiative is designed to improve research productivity by building consensus. Stakeholders involved with peritoneal dialysis (PD) have put life participation on top of their list, along with treatment-related infection, cardiovascular complications, and patient and techn...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2019.05.002
更新日期：2019-09-01 00:00:00
abstract：:Phagocyte-derived interleukin-12 (IL-12) is a key cytokine that induces the development of an effective Th1 type immune response in various inflammatory and infectious disorders. To determine the importance of IL-12 in the pathogenesis of autoimmune renal injury we examined the renal production of this heterodimeric c...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1997.10
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND:Redox-active iron can promote oxidative stress and tissue injury by catalyzing hydroxyl radical generation and lipid peroxidation. Intravenous iron preparations are routinely administered in conjunction with erythropoietin to treat anemia in patients with chronic renal failure (CRF), a condition that is mark...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2004.00580.x
更新日期：2004-05-01 00:00:00
abstract：:Cells from RCCT-28A cell line, which exhibited the highest peanut-lectin binding capacity [RCCT-28A(P+)] were separated and cultured attempting to obtain a population of HCO3- secreting cells. The mechanisms of apical solution acid or base extrusion were studied in confluent monolayers. In RCCT-28A (P+) cells net H+ f...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1993.30
更新日期：1993-01-01 00:00:00
abstract：:Vitamin D deficiency is common in patients with chronic kidney disease after renal transplantation. Vitamin D, essential for mineral and bone metabolism, also has myriad beneficial autocrine effects on intact immune responses and defense against infection, as well as suppression of malignant changes. Supplementation w...
journal_title：Kidney international
pub_type： 评论,杂志文章,评审
doi：10.1038/ki.2008.492
更新日期：2009-03-01 00:00:00
abstract：:Bone-derived fibroblast growth factor-23 (FGF23) plays an important role in systemic phosphate turnover. Increased FGF23 activity results in hypophosphatemic disorders, while reduced activity is linked to hyperphosphatemic disorders. FGF23, together with klotho as co-factor, can activate FGF receptors in its target ti...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2014.316
更新日期：2014-12-01 00:00:00
abstract：:Acute kidney injury (AKI) activates pathways of cell death and cell proliferation. Although seemingly discrete and unrelated mechanisms, these pathways can now be shown to be connected and even to be controlled by similar pathways. The dependence of the severity of renal-cell injury on cell cycle pathways can be used ...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.2009.224
更新日期：2009-09-01 00:00:00
abstract：:The kallikrein-kininogen-kinin system (KKK) has been implicated in the renal sodium excretion response to changes in dietary sodium. However, both increases and decreases in the activity of this system have been observed when urinary sodium excretion is augmented by a variety of maneuvers. To further evaluate the pote...
journal_title：Kidney international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1038/ki.1987.74
更新日期：1987-03-01 00:00:00
abstract：BACKGROUND:Cardiovascular death continues to be a major problem in renal failure. Structural abnormalities of the heart and the vasculature contribute to the increased cardiovascular risk. They are ameliorated by angiotensin-converting enzyme (ACE) inhibitors, but because of the nonspecifity of ACE inhibition, it is un...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2000.00163.x
更新日期：2000-07-01 00:00:00
abstract：:The development of effective therapies for acute kidney injury (AKI) has been hindered by delayed diagnosis in the clinical setting, varying definitions of AKI, and limited prognostic information. In a study by Portilla et al., elevations in liver fatty acid-binding protein (L-FABP) were found to predict AKI in childr...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5002739
更新日期：2008-02-01 00:00:00
abstract：:We present a new hypothesis to explain the development of salt-dependent hypertension in humans. We propose that hypertension has two phases: an early phase in which elevations in blood pressure (BP) are mainly episodic and are mediated by a hyperactive sympathetic nervous or renin-angiotensin system, and a second pha...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.1997.442
更新日期：1997-11-01 00:00:00
abstract：:We investigated the effect of the potent immunosuppressive agent, FK506, on experimental glomerular thrombosis in rats by combined injections of nephrotoxic serum (NTS) and lipopolysaccharide (LPS). Either FK506 or placebo was administered intramuscularly three hours prior to injection of NTS that was followed one hou...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1994.207
更新日期：1994-06-01 00:00:00
