Localization of [14C] labeled anti-streptococcal cell membrane monoclonal antibodies (anti-SCM mAb) in mice.


:Six different hybridomas secreting anti-SCM mAb and one control mAb were placed into adult mice along with [14C] amino acids for biosynthetically labeling. After sacrifice, the 14C mAb ascites along with serum, heart, kidney, lung and skeletal muscle were recovered. Tissue associated specific radio-activity (SpAc) and microscopic structural analyses were performed. Confirmation of mAb specificity was by both immunodot blots as well as Western blot analysis. Peritoneal injection of measured doses of anti-SCM mAb yielded tissue SpAc confirming their in vitro specificities. Two of the mAb showed strong reactivity to both renal (GBM) and lung basement membrane (LBM), two were mainly GBM reactive and two showed polyreactivity with a marked reactivity to a Z-line antigen. Autoradiographic light microscopy confirmed that the anti-SCM mAbs bound to both GBM and LBM and to Z-line antigen. Titrated doses of the mAb yielded autoradiographic confirmation in which the grain number on the GBM and LBM increased with increasing dose of mAb for all mAb except the control. This effect was not seen in the muscle tissues but anatomical localization at the Z-line was consistent. The major significance of these studies is the demonstration that circulating antibodies to SCM can react in vivo with normal mammalian antigens adding confirmation to the in vitro specificity of these cross-reactive anti-SCM mAbs.






Lange CF




Has Abstract


1994-01-01 00:00:00












  • Distinct role of T helper Type 17 immune response for Graves' hyperthyroidism in mice with different genetic backgrounds.

    abstract::T helper type 17 (Th17) cells, a newly identified effector T-cell subset, have recently been shown to play a role in numerous autoimmune diseases, including iodine-induced autoimmune thyroiditis in non-obese diabetic (NOD)-H2(h4) mice, which had previously been thought Th1-dominant. We here studied the role of Th17 in...


    pub_type: 杂志文章


    authors: Horie I,Abiru N,Saitoh O,Ichikawa T,Iwakura Y,Eguchi K,Nagayama Y

    更新日期:2011-03-01 00:00:00

  • HLA-DP-positive T cells in patients with systemic lupus erythematosus.

    abstract::HLA-DP+ T cells in peripheral blood from 23 patients with systemic lupus erythematosus (SLE) were examined using two-colour flow cytometry analysis. A marked increase of HLA-DP+ T cells was observed in patients with SLE (20.5-98.7%; 59.8 +/- 20.8%) in comparison to normal subjects (1.3-20.6%; 11.1 +/- 7.2%), and the r...


    pub_type: 杂志文章


    authors: Tokano Y,Hishikawa T,Hirose T,Sekigawa I,Hashimoto H,Okumura K,Hirose S

    更新日期:1990-01-01 00:00:00

  • Prolactin modulates the incidence of diabetes in male and female NOD mice.

    abstract::The nonobese diabetic (NOD) mouse develops diabetes spontaneously due to autoimmune destruction of the pancreatic islets with a higher incidence in the female than the male. Prolactin (PRL), a hormone whose role has been previously focused on reproduction and lactation has been demonstrated to influence immune respons...


    pub_type: 杂志文章


    authors: Hawkins TA,Gala RR,Dunbar JC

    更新日期:1994-01-01 00:00:00

  • The association between three promoter polymorphisms of IL-10 and inflammatory bowel diseases (IBD): a meta-analysis.

    abstract:AIM:To assess the relationship of the Interleukin-10 (IL-10) -1082G/A (rs1800896), -819C/T (rs1800871) and -592C/A (rs1800872) polymorphism with inflammatory bowel disease (IBD) by means of meta-analysis. METHODS:Published data addressing the association between polymorphism of the IL-10 with Crohn's disease (CD) and ...


    pub_type: 杂志文章,meta分析


    authors: Zou L,Wang L,Gong X,Zhao H,Jiang A,Zheng S

    更新日期:2014-02-01 00:00:00

  • Treatment with anti-C5aR mAb leads to early-onset clinical and mechanistic effects in the murine delayed-type hypersensitivity arthritis model.

    abstract::Blockade of the complement cascade at the C5a/C5a receptor (C5aR)-axis is believed to be an attractive treatment avenue in rheumatoid arthritis (RA). However, the effects of such interventions during the early phases of arthritis remain to be clarified. In this study we use the murine delayed-type hypersensitivity art...


    pub_type: 杂志文章


    authors: Atkinson SM,Nansen A,Usher PA,Sondergaard BC,Mackay CR,Friedrichsen B,Chang CC,Tang R,Skov S,Haase C,Hornum L

    更新日期:2015-01-01 00:00:00

  • Role of CD5+ B-1 cells in EAE pathogenesis.

    abstract::Hybridoma cell lines producing natural autoantibodies (NAA), generated from A.SW mice with progressive experimental autoimmune encephalomyelitis (P-EAE), have been shown to cause demyelination and renal pathology when injected into naive mice. To investigate the relative contribution of these antibodies to disease pat...


    pub_type: 杂志文章


    authors: Peterson LK,Tsunoda I,Fujinami RS

    更新日期:2008-08-01 00:00:00

  • Pemphigus autoimmunity: hypotheses and realities.

    abstract::The goal of contemporary research in pemphigus vulgaris and pemphigus foliaceus is to achieve and maintain clinical remission without corticosteroids. Recent advances of knowledge on pemphigus autoimmunity scrutinize old dogmas, resolve controversies, and open novel perspectives for treatment. Elucidation of intimate ...


    pub_type: 杂志文章,评审


    authors: Grando SA

    更新日期:2012-02-01 00:00:00

  • Two SNPs in NLRP3 gene are involved in the predisposition to type-1 diabetes and celiac disease in a pediatric population from northeast Brazil.

    abstract::Recent findings provide evidence of the critical role of innate immunity NALP1/NLRP1 and NALP3/NLRP3/CIAS1 genes in inflammatory diseases, and also in the predisposition to autoimmune disorders. We evaluated the possible association of five single nucleotide polymorphisms (SNPs), two in NLRP1 gene and three in NLRP3 g...


    pub_type: 杂志文章


    authors: Pontillo A,Brandao L,Guimaraes R,Segat L,Araujo J,Crovella S

    更新日期:2010-12-01 00:00:00

  • Nature of 64 kDa eye muscle and thyroid membrane proteins and their significance in thyroid-associated ophthalmopathy--an hypothesis.

    abstract::Although a variety of eye muscle antigens are recognized by autoantibodies in the serum from patients with thyroid associated ophthalmopathy (TAO) 64 kDa membrane proteins, which are also expressed in the thyroid, are most closely linked to the development of ophthalmopathy in patients with autoimmune thyroid disease....


    pub_type: 杂志文章,评审


    authors: Boucher A,Bernard N,Zhang ZG,Rodien P,Salvi M,Wall JR

    更新日期:1993-01-01 00:00:00

  • Both early and late apoptotic blebs are taken up by DC and induce IL-6 production.

    abstract::During apo blebs, containing nuclear components, are formed at the cells' surfaces. When these blebs separate from the dying cell an apo cell body remains. The contents of apo blebs are modified and can be released, especially in patients with systemic lupus erythematosus (SLE) since impaired clearance of apo material...


    pub_type: 杂志文章


    authors: Fransen JH,Hilbrands LB,Jacobs CW,Adema GJ,Berden JH,Van der Vlag J

    更新日期:2009-05-01 00:00:00

  • Concentric fibrosis and cellular infiltration around bile ducts induced by graft-versus-host reaction in mice: a role of CD8+ cells.

    abstract::We report in this paper on obvious fibrotic lesions in the liver of mice undergoing specified graft-versus-host reaction (GVHR). B6 CD8+ splenocytes were transferred into (bm 12 x bm 1)F1 mice to induce GVHR. Recipient mice had been thymectomized and administrated with anti-CD8 monoclonal antibody (mAb) to deplete CD8...


    pub_type: 杂志文章


    authors: Inada S,Suzuki K,Kimura T,Hayashi A,Narita T,Yui R,Asakura H,Fujiwara M

    更新日期:1995-01-01 00:00:00

  • Definitions of and contributions to cardiovascular disease in systemic lupus erythematosus.

    abstract::Patients with systemic lupus erythematosus (SLE) have a significantly increased risk of cardiovascular disease (CVD). Increased prevalence of atherosclerosis may explain part of this enhanced risk, but SLE related CVD can also result from other mechanisms. Vascular events may be the result of several pathophysiologic ...


    pub_type: 杂志文章,评审


    authors: Gustafsson JT,Svenungsson E

    更新日期:2014-03-01 00:00:00

  • Aβ anti-idiotypic antibodies are present in intravenous immunoglobulin and are produced in mice following its administration.

    abstract::The effects of intravenous immunoglobulin (IVIG) products were recently examined in patients with Alzheimer's disease (AD). Although encouraging results were obtained in pilot studies, later trials produced negative results. The rationale for these studies was that IVIG contains antibodies to amyloid-beta (Aβ). Howeve...


    pub_type: 杂志文章


    authors: Loeffler DA,Klaver AC,Coffey MP

    更新日期:2015-06-01 00:00:00

  • Different islet protein expression profiles during spontaneous diabetes development vs. allograft rejection in BB-DP rats.

    abstract::Type 1 diabetes (T1D) is characterized by selective autoimmune destruction of the insulin producing beta-cells in the islets of Langerhans. When the beta-cells are destroyed exogenous administration of insulin is necessary for maintenance of glucose homeostasis. Allogeneic islet transplantation has been used as a mean...


    pub_type: 杂志文章


    authors: Christensen UB,Larsen PM,Fey S,Karlsen AE,Pociot F,Nerup J,Sparre T

    更新日期:2006-06-01 00:00:00

  • Dysregulation of p56lck kinase in patients with systemic lupus erythematosus.

    abstract::In this study we investigated one of the possible mechanisms of p56lck down-regulation in peripheral blood lymphocytes (PBLs) from Systemic Lupus Erythematosus (SLE) patients and we correlated p56lck dysregulation with accelerated apoptosis in SLE PBLs. PBLs from SLE patients and healthy donors were isolated. p56lck p...


    pub_type: 杂志文章


    authors: Matache C,Onu A,Stefanescu M,Tanaseanu S,Dragomir C,Dolganiuc A,Szegli G

    更新日期:2001-01-01 00:00:00

  • Pathogenic mechanisms in systemic lupus erythematosus.

    abstract::Systemic lupus erythematosus (SLE) is a chronic inflammatory disease characterized by the dysfunction of T cells, B cells, and dendritic cells and by the production of antinuclear autoantibodies. This editorial provides a synopsis of newly discovered genetic factors and signaling pathways in lupus pathogenesis that ar...


    pub_type: 社论


    authors: Perl A

    更新日期:2010-02-01 00:00:00

  • Stress-induced cell surface expression and antigenic alteration of the Ro/SSA autoantigen.

    abstract::Recent studies have shown that Ro/SSA autoantigen is heterogeneous. There are two isoform families; the 60 kD forms (Ro60) and the 52 kD forms (Ro52). Recently we have found that autoantibodies to the Ro/SSA proteins are conformation dependent. Anti-Ro60 antibodies are mainly directed to the native protein and convers...


    pub_type: 杂志文章


    authors: Igarashi T,Itoh Y,Fukunaga Y,Yamamoto M

    更新日期:1995-01-01 00:00:00

  • The origin of extracellular DNA during the clearance of dead and dying cells.

    abstract::DNA is a nuclear molecule that has both an intracellular and extracellular role. Inside the cell, it is the essential molecule of heredity while outside the cell it can have immunological activity, both alone and in the context of immune complexes. Furthermore, extracellular DNA has information content that can be min...


    pub_type: 杂志文章,评审


    authors: Pisetsky DS,Fairhurst AM

    更新日期:2007-06-01 00:00:00

  • Effect of passive transfer of human anti-myelin-associated glycoprotein IgM in marmoset.

    abstract::Adult and one week old marmosets were injected intravenously within a one month period or intraperitoneally within a 6 week period respectively, with monoclonal IgM having an anti-myelin associated glycoprotein antibody activity. No clinical or electrophysiological abnormalities could be detected in experimental anima...


    pub_type: 杂志文章


    authors: Dancea S,Dellagi K,Renaud F,Mahouy G,Hauw JJ,Brouet JC

    更新日期:1989-01-01 00:00:00

  • CTLA4Ig prevents initiation but not evolution of anti-phospholipid syndrome in NZW/BXSB mice.

    abstract::NZW "x" BXSB F1 mice develop SLE that is associated with an anti-phospholipid syndrome characterized by anti-cardiolipin antibodies, thrombocytopenia and small coronary artery thrombosis. This syndrome is immune mediated and, dependent, on CD4+T cells. To determine whether disease in these mice can be treated with blo...


    pub_type: 杂志文章


    authors: Akkerman A,Huang W,Wang X,Ramanujam M,Schiffer L,Madaio M,Factor SM,Davidson A

    更新日期:2004-09-01 00:00:00

  • Nitration of H2B histone elicits an immune response in experimental animals.

    abstract::Histone H2B is an autoantigen that appears in circulation due to altered apoptosis/or insufficient clearance and is likely to be involved in the induction and progression of autoimmune diseases since modified-H2B is immunogenic. Our studies demonstrate that tyrosines of H2B histone spontaneously converts to free and n...


    pub_type: 杂志文章


    authors: Khan MA,Alam K,Hassan SM,Rizvi MMA

    更新日期:2017-06-01 00:00:00

  • Improvement of cardiac function after immunoadsorption in patients with dilated cardiomyopathy.

    abstract::Dilated cardiomyopathy (DCM) is a myocardial disease characterized by progressive depression of myocardial contractile function and by ventricular dilatation. Abnormalities of the cellular and humoral immune system are present in patients with myocarditis and DCM. Various circulating cardiac autoantibodies have been d...


    pub_type: 杂志文章,评审


    authors: Felix SB,Staudt A,Friedrich GB

    更新日期:2001-01-01 00:00:00

  • A modified superantigen rescues Ly6G- CD11b+ blood monocyte suppressor function and suppresses antigen-specific inflammation in EAE.

    abstract::In a previous study, we showed that the Ly6G(-)CD11b(+) blood monocytes residing in naïve mice are intrinsically immunosuppressive and that loss of this suppressive function may contribute to the development of autoimmunity in experimental autoimmune encephalomyelitis (EAE), a murine model of human multiple sclerosis....


    pub_type: 杂志文章


    authors: Slaney CY,Toker A,Fraser JD,Harper JL,Bäckström BT

    更新日期:2013-06-01 00:00:00

  • In vivo migration of tonsil lymphocytes in rheumatoid synovial tissue engrafted in SCID mice: involvement of LFA-1.

    abstract::Integrin-adressin binding is a critical step in lymphocte attachment to target tissues. The mucosal recognition systems (alpha E beta 7, alpha 4 beta 7, MADcam-1) have been implicated in the autoimmune process in rheumatoid arthritis. We developed a model for in vivo study of radio-labelled lymphocyte circulation and ...


    pub_type: 杂志文章


    authors: Jorgensen C,Couret I,Canovas F,Bologna C,Brochier J,Reme T,Sany J

    更新日期:1996-01-01 00:00:00

  • Polymorphisms in NLRP1 gene and susceptibility to autoimmune thyroid disease.

    abstract::The autoimmune thyroid disorders, or AITDs, comprise 2 related disorders, Graves' disease and Hashimoto thyroiditis. In AITD, immune system produces antibodies against autothyroid antigens. The etiology of AITDs involves a complex interaction between genetic predisposing factors and environmental triggering factors. V...


    pub_type: 杂志文章


    authors: Alkhateeb A,Jarun Y,Tashtoush R

    更新日期:2013-05-01 00:00:00

  • Carbonic anhydrase III: a new target for autoantibodies in autoimmune diseases.

    abstract::The objective of this study was to identify new autoantibodies that could be useful for the diagnosis of rheumatoid arthritis (RA) using immunoblotting on synovial membrane proteins which represent the best source of candidate RA autoantigens. A new target protein with a molecular weight of 26 kDa was found to be reco...


    pub_type: 杂志文章


    authors: Robert-Pachot M,Desbos A,Moreira A,Becchi M,Tebib J,Bonnin M,Aitsiselmi T,Bienvenu J,Fabien N

    更新日期:2007-07-01 00:00:00

  • Novel aspects on the contribution of T cells and dendritic cells in the pathogenesis of myositis.

    abstract::This review focuses on recent advances and new hypothesis in the understanding of the T and dendritic cells contribution to the pathogenesis of idiopathic inflammatory myopathies (IIMs), especially polymyositis (PM) and dermatomyositis (DM). The new data show that non-specific amplification of muscle inflammation by T...


    pub_type: 杂志文章,评审


    authors: Chevrel G,Page G,Miossec P

    更新日期:2006-05-01 00:00:00

  • CTLs' repertoire shaping in the thymus: a Monte Carlo simulation.

    abstract:MOTIVATION:The human immune system evolved a multi-layered control mechanism to eliminate self-reactive cells. Of these so-called tolerance induction mechanisms, lymphocytes T education in the thymus gland represents the very first one. This complicated process is not fully understood and quantitative models able to he...


    pub_type: 杂志文章


    authors: Castiglione F,Santoni D,Rapin N

    更新日期:2011-06-01 00:00:00

  • Autoreactive cytotoxic T lymphocytes in pemphigus and pemphigoid.

    abstract::In order to know whether effector cells are capable of recognizing the epidermal targets in pemphigus vulgaris (PV) and bullous pemphigoid (BP), non-adherent cells (NAC) of peripheral blood from 27 primary PV, 19 BP patients and 12 healthy volunteers were used in cytotoxic tests. Autologous, allogenic and murine epide...


    pub_type: 杂志文章


    authors: Grando SA,Glukhenky BT,Drannik GN,Kostromin AP,Boiko YYa,Senyuk OF

    更新日期:1989-01-01 00:00:00

  • Expression of major histocompatibility complex class II antigen in NOD mouse thyroid.

    abstract::Non obese diabetic (NOD) mice spontaneously develop thyroiditis in addition to diabetes. Mononuclear cells begin to infiltrate the thyroid of these animals in the first month of life. The expression of major histocompatibility complex (MHC) class II (Ia) antigens by cells in the thyroid from NOD mice of various ages w...


    pub_type: 杂志文章


    authors: Margolese H,O'Keefe C,Chung F,Ertug F,Wall JR,Bernard NF

    更新日期:1994-01-01 00:00:00