Abstract:
:We have used an SV40-based shuttle vector, pZ189, to investigate the capacity of HeLa cell extracts to reproduce the in vivo process of mutation fixation. We showed previously that when UV-irradiated pZ189 is replicated in these extracts, bypass of UV photoproducts occurs, resulting in base substitution mutations in the supF gene of the vector. Here we report the DNA sequence characterization of a collection of 60 of these UV-induced mutants. Most of the mutations observed are single or tandem double base substitutions at dipyrimidine sites; of these, approximately 90% are G:C-->A:T transitions. Mutations are observed predominantly at a few sites, in particular at positions 155 and 156 in the supF sequence. No dramatic differences in the mutation spectrum were observed when the orientation of the supF gene was reversed with respect to the SV40 origin of replication, suggesting that mutation fixation occurs similarly on both the leading and the lagging strands for DNA replication. Generally, the mutational hot spots observed in vitro are at the same sites as those observed when UV-irradiated pZ189 was passaged in human or monkey cells in culture. Thus, it appears that the replication and mutagenesis of UV-damaged templates in HeLa cell extracts accurately reflects these processes in the intact cell.
journal_name
Environ Mol Mutagenjournal_title
Environmental and molecular mutagenesisauthors
Carty MP,el-Saleh S,Zernik-Kobak M,Dixon Kdoi
10.1002/em.2850260207subject
Has Abstractpub_date
1995-01-01 00:00:00pages
139-46issue
2eissn
0893-6692issn
1098-2280journal_volume
26pub_type
杂志文章abstract::Griseofulvin is an orally administered antifungal drug that affects microtubule formation in vitro and interferes with microtubule dynamics in vivo as clearly shown for mitotic cells in several cell systems. This article reports the effects of griseofulvin on in vitro maturation of porcine oocytes and subsequent effec...
journal_title:Environmental and molecular mutagenesis
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journal_title:Environmental and molecular mutagenesis
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journal_title:Environmental and molecular mutagenesis
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journal_title:Environmental and molecular mutagenesis
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journal_title:Environmental and molecular mutagenesis
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doi:10.1002/em.20444
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journal_title:Environmental and molecular mutagenesis
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journal_title:Environmental and molecular mutagenesis
pub_type: 杂志文章
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journal_title:Environmental and molecular mutagenesis
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journal_title:Environmental and molecular mutagenesis
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journal_title:Environmental and molecular mutagenesis
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journal_title:Environmental and molecular mutagenesis
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更新日期:1998-01-01 00:00:00
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journal_title:Environmental and molecular mutagenesis
pub_type: 杂志文章,评审
doi:10.1002/em.10061
更新日期:2002-01-01 00:00:00
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journal_title:Environmental and molecular mutagenesis
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journal_title:Environmental and molecular mutagenesis
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更新日期:1999-01-01 00:00:00
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journal_title:Environmental and molecular mutagenesis
pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
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journal_title:Environmental and molecular mutagenesis
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journal_title:Environmental and molecular mutagenesis
pub_type: 杂志文章
doi:10.1002/em.2850140403
更新日期:1989-01-01 00:00:00
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journal_title:Environmental and molecular mutagenesis
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更新日期:1997-01-01 00:00:00
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journal_title:Environmental and molecular mutagenesis
pub_type: 杂志文章,评审
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更新日期:2006-06-01 00:00:00