当前位置： SCI文献检索 > BRITISH JOURNAL OF CANCER期刊下所有文献 > DT-diaphorase activity in normal and neoplastic human tissues; an indicator for sensitivity to bioreductive agents?

DT-diaphorase activity in normal and neoplastic human tissues; an indicator for sensitivity to bioreductive agents?

Abstract：

:DT-diaphorase (DTD) is an important enzyme for the bioreductive activation of the new alkylating indoloquinone EO9. In preclinical studies, EO9 has shown selective anti-tumour activity against solid tumours and under hypoxic conditions. The levels of three reductive enzymes have been determined in three types of human solid tumours, together with corresponding normal tissues and normal liver. DTD enzyme activities were measured in tumour extracts using 2,6-dichlorophenolindophenol (DCPIP) and NADH as substrates; cytochrome P450 reductase or cytochrome b5 reductase activities were assessed with cytochrome c and NADPH or NADH respectively. DTD activity was highest in non-small-cell lung (NSCLC)-tumours (mean 123 nmol DCPIP min-1 mg-1), followed by colon carcinoma (mean 75 nmol min-1 mg-1) and squamous cell carcinoma of the head and neck (6-fold lower than NSCLC). DTD activity was very low in normal liver and normal lung (4-6 nmol min-1 mg-1), while the levels in normal colon mucosa or normal mucosa of the head and neck region were in the same range as the corresponding tumours. The levels of the two other reductive enzymes, cytochrome P450 reductase (CP450R) and cytochrome b5 reductase (Cb5R), were 5 to 25-fold lower than those of DTD in all the tissues, except for normal liver, in which DTD was 2 to 4-fold lower. The degree of variation found for DTD (range 4-250 nmol min-1 mg-1), was not observed for these enzymes (CP450R, 0.8-7.8 nmol cytochrome c min-1 mg-1; Cb5R, 3.5-27.6 nmol min-1 mg-1).(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Br J Cancer

journal_title

British journal of cancer

authors

Smitskamp-Wilms E,Giaccone G,Pinedo HM,van der Laan BF,Peters GJ

doi

10.1038/bjc.1995.433

subject

Has Abstract

pub_date

1995-10-01 00:00:00

pages

917-21

issue

4

eissn

0007-0920

issn

1532-1827

journal_volume

72

pub_type

杂志文章

相关文献

BRITISH JOURNAL OF CANCER文献大全
  • Gene and pathway level analyses of germline DNA-repair gene variants and prostate cancer susceptibility using the iCOGS-genotyping array.

    abstract：BACKGROUND:Germline mutations within DNA-repair genes are implicated in susceptibility to multiple forms of cancer. For prostate cancer (PrCa), rare mutations in BRCA2 and BRCA1 give rise to moderately elevated risk, whereas two of B100 common, low-penetrance PrCa susceptibility variants identified so far by genome-wid...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.2016.50

    authors： Saunders EJ,Dadaev T,Leongamornlert DA,Al Olama AA,Benlloch S,Giles GG,Wiklund F,Gronberg H,Haiman CA,Schleutker J,Nordestgaard BG,Travis RC,Neal D,Pasayan N,Khaw KT,Stanford JL,Blot WJ,Thibodeau SN,Maier C,Kibel AS

    更新日期：2016-04-12 00:00:00

  • HuR is exported to the cytoplasm in oral cancer cells in a different manner from that of normal cells.

    abstract：:HuR, a ubiquitously expressed member of the Hu protein family that binds and stabilizes an AU-rich element (ARE)-containing mRNAs, is known to shuttle between the nucleus and the cytoplasm via several export pathways. When normal cells were treated with heat shock, HuR was exported to the cytoplasm in a chromosome mai...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/sj.bjc.6605084

    authors： Hasegawa H,Kakuguchi W,Kuroshima T,Kitamura T,Tanaka S,Kitagawa Y,Totsuka Y,Shindoh M,Higashino F

    更新日期：2009-06-16 00:00:00

  • Stressful life events and cancer risk.

    abstract：:In a prospective cohort study in Denmark of 8736 randomly selected people, no evidence was found among 1011 subjects who developed cancer that self-reported stressful major life events had increased their risk for cancer. ...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/sj.bjc.6603471

    authors： Bergelt C,Prescott E,Grønbaek M,Koch U,Johansen C

    更新日期：2006-12-04 00:00:00

  • Assessing the function of homologous recombination DNA repair in malignant pleural effusion (MPE) samples.

    abstract：BACKGROUND:Patients with malignant pleural effusions (MPEs) generally have advanced disease with poor survival and few therapeutic options. Cells within MPEs may be used to stratify patients for targeted therapy. Targeted therapy with poly(ADP ribose) polymerase inhibitors (PARPi) depends on identifying homologous reco...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.2014.261

    authors： Patterson MJ,Sutton RE,Forrest I,Sharrock R,Lane M,Kaufmann A,O'Donnell R,Edmondson RJ,Wilson BT,Curtin NJ

    更新日期：2014-07-08 00:00:00

  • Quantitative aspects of accelerated nuclear polyploidization and tumour formation in dieldrin treated CF-1 mouse liver.

    abstract：:Nuclear polyploidization in the livers of CF-1 mice, exposed to dieldrin (0, 1, 5 and 10 ppm in the diet), was studied up to the median time of liver tumour development (ranging from 15 to 27 months) in the respective treatment groups. In untreated controls nuclear polyploidization is characterized by a linear increas...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1988.160

    authors： van Ravenzwaay B,Kunz W

    更新日期：1988-07-01 00:00:00

  • A phase I dose-finding and pharmacokinetic study of subcutaneous semisynthetic homoharringtonine (ssHHT) in patients with advanced acute myeloid leukaemia.

    abstract：:To determine the maximum-tolerated dose (MTD), dose-limiting toxicities and pharmacokinetic of semisynthetic homoharringtonine (ssHHT), given as a twice daily subcutaneous (s.c.) injections for 9 days, in patients with advanced acute leukaemia, 18 patients with advanced acute myeloid leukaemia were included in this se...

    journal_title：British journal of cancer

    pub_type： 杂志文章,随机对照试验

    doi：10.1038/sj.bjc.6603265

    authors： Lévy V,Zohar S,Bardin C,Vekhoff A,Chaoui D,Rio B,Legrand O,Sentenac S,Rousselot P,Raffoux E,Chast F,Chevret S,Marie JP

    更新日期：2006-08-07 00:00:00

  • Results from a seven-year programme of breast self-examination in 89,010 women.

    abstract：:This report presents the results of a study into the effect of breast self-examination (BSE) in a large defined population within the City of Nottingham since 1979. We have examined the effect of breast self-examination in a group of patients invited to attend for education in BSE compared with a group of historical c...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1989.294

    authors： Locker AP,Caseldine J,Mitchell AK,Blamey RW,Roebuck EJ,Elston CW

    更新日期：1989-09-01 00:00:00

  • Circulating intercellular adhesion molecule-1 (ICAM-1), E-selectin and vascular cell adhesion molecule-1 (VCAM-1) in human malignancies.

    abstract：:Cellular adhesion molecules have been implicated in tumour progression and metastasis. This study examines for the first time the serum concentrations of circulating VCAM-1 and E-selectin in a consecutive series of 110 cancer patients seen in a general medical oncology clinic, and confirms and extends previous studies...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1993.298

    authors： Banks RE,Gearing AJ,Hemingway IK,Norfolk DR,Perren TJ,Selby PJ

    更新日期：1993-07-01 00:00:00

  • Midkine as a prognostic biomarker in oral squamous cell carcinoma.

    abstract：:The aim of this study was to evaluate serum midkine (S-MK) concentrations as a prognostic tumour marker in oral squamous cell carcinoma (OSCC). We measured S-MK concentrations in patients with OSCC and healthy volunteers. In addition, we performed real-time quantitative reverse transcription-PCR analysis and immunohis...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/sj.bjc.6604539

    authors： Ota K,Fujimori H,Ueda M,Shiniriki S,Kudo M,Jono H,Fukuyoshi Y,Yamamoto Y,Sugiuchi H,Iwase H,Shinohara M,Ando Y

    更新日期：2008-08-19 00:00:00

  • Genistein downregulates onco-miR-1260b and inhibits Wnt-signalling in renal cancer cells.

    abstract：BACKGROUND:Wnt-signalling has an important role in renal cancer and it is modulated by genistein in other cancers. Recently, microRNAs (miRNAs) have emerged as new regulators of gene expression. Thus, we focused on miRNAs to examine the regulatory mechanism of genistein on the Wnt-signalling pathway in renal cell carci...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.2013.173

    authors： Hirata H,Ueno K,Nakajima K,Tabatabai ZL,Hinoda Y,Ishii N,Dahiya R

    更新日期：2013-05-28 00:00:00

  • Morphological and metastatic murine melanoma variants: motility, adhesiveness, cell surface and in vivo properties.

    abstract：:The behaviour in vivo of tight and loose variants of murine melanoma cells is further characterized. In vitro clonal morphology is reproduced on a variety of substrates. Results suggest that repeated selection of loose cells can co-select for cells with high metastatic and colonization potentials. Measurement of cell ...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1987.244

    authors： Clark SR,Brody JS,Sidebottom E

    更新日期：1987-11-01 00:00:00

  • Diagnostic value of protein induced by vitamin K absence (PIVKAII) and hepatoma-specific band of serum gamma-glutamyl transferase (GGTII) as hepatocellular carcinoma markers complementary to alpha-fetoprotein.

    abstract：:Serum protein induced by vitamin K absence or antagonist II (PIVKAII), hepatoma-specific band of serum gamma-glutamyl transferase (GGTII), and alpha-fetoprotein (AFP) levels were determined in 120 patients with hepatocellular carcinoma (HCC) and 90 patients with cirrhosis. The mean serum concentration of PIVKAII in HC...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/sj.bjc.6601018

    authors： Cui R,He J,Zhang F,Wang B,Ding H,Shen H,Li Y,Chen X

    更新日期：2003-06-16 00:00:00

  • Expression pattern of tumour-associated antigens in hepatocellular carcinoma: association with immune infiltration and disease progression.

    abstract：BACKGROUND:The distinct expression pattern of tumour-associated antigens (TAAs) might be a critical reason for the inefficacy of immunity-based treatments and heterogeneous postsurgical recovery in patients with solid tumours, including hepatocellular carcinoma (HCC). However, little is known about the clinical value o...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.2013.390

    authors： Liang J,Ding T,Guo ZW,Yu XJ,Hu YZ,Zheng L,Xu J

    更新日期：2013-08-20 00:00:00

  • Identification of molecular mechanisms for cellular drug resistance by combining drug activity and gene expression profiles.

    abstract：:Acquired drug resistance is a major problem in cancer treatment. To explore the genes involved in chemosensitivity and resistance, 10 human tumour cell lines, including parental cells and resistant subtypes selected for resistance against doxorubicin, melphalan, teniposide and vincristine, were profiled for mRNA expre...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/sj.bjc.6602699

    authors： Rickardson L,Fryknäs M,Dhar S,Lövborg H,Gullbo J,Rydåker M,Nygren P,Gustafsson MG,Larsson R,Isaksson A

    更新日期：2005-08-22 00:00:00

  • Aerosolised 5-azacytidine suppresses tumour growth and reprogrammes the epigenome in an orthotopic lung cancer model.

    abstract：BACKGROUND:Epigenetic silencing by promoter methylation and chromatin remodelling affects hundreds of genes and is a causal event for lung cancer. Treatment of patients with low doses of the demethylating agent 5-azacytidine in combination with the histone deacetylase inhibitor entinostat has yielded clinical responses...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.2013.575

    authors： Reed MD,Tellez CS,Grimes MJ,Picchi MA,Tessema M,Cheng YS,March TH,Kuehl PJ,Belinsky SA

    更新日期：2013-10-01 00:00:00

  • Expression of two WFDC1/ps20 isoforms in prostate stromal cells induces paracrine apoptosis through regulation of PTGS2/COX-2.

    abstract：BACKGROUND:WFDC1/Prostate stromal 20 (ps20) is a small secreted protein highly expressed within the prostate stroma. WFDC1/ps20 expression is frequently downregulated or lost in prostate cancer (PCa) and ps20 has demonstrated growth-suppressive functions in numerous tumour model systems, although the mechanisms of this...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.2016.91

    authors： Hickman OJ,Smith RA,Dasgupta P,Rao SN,Nayak S,Sreenivasan S,Vyakarnam A,Galustian C

    更新日期：2016-05-24 00:00:00

  • Prognostic markers in cancer: the evolution of evidence from single studies to meta-analysis, and beyond.

    abstract：:In oncology, prognostic markers are clinical measures used to help elicit an individual patient's risk of a future outcome, such as recurrence of disease after primary treatment. They thus facilitate individual treatment choice and aid in patient counselling. Evidence-based results regarding prognostic markers are the...

    journal_title：British journal of cancer

    pub_type： 杂志文章,评审

    doi：10.1038/sj.bjc.6604999

    authors： Riley RD,Sauerbrei W,Altman DG

    更新日期：2009-04-21 00:00:00

  • Multivariate regression analyses of data from a randomised, double-blind, placebo-controlled study confirm quality of life benefit of epoetin alfa in patients receiving non-platinum chemotherapy.

    abstract：:Cancer-related anaemia is associated with a wide spectrum of symptoms that can negatively affect quality of life. Because epoetin alfa has demonstrated efficacy in correcting cancer-related anaemia, the impact of this treatment on quality of life was evaluated in a multinational, randomised, double-blind, placebo-cont...

    journal_title：British journal of cancer

    pub_type： 临床试验,杂志文章,多中心研究,随机对照试验

    doi：10.1038/sj.bjc.6600657

    authors： Fallowfield L,Gagnon D,Zagari M,Cella D,Bresnahan B,Littlewood TJ,McNulty P,Gorzegno G,Freund M,Epoetin Alfa Study Group.

    更新日期：2002-12-02 00:00:00

  • Fruit and vegetable consumption in relation to hepatocellular carcinoma in a multi-centre, European cohort study.

    abstract：BACKGROUND:Vegetable and/or fruit intakes in association with hepatocellular carcinoma (HCC) risk have been investigated in case-control studies conducted in specific European countries and cohort studies conducted in Asia, with inconclusive results. No multi-centre European cohort has investigated the indicated associ...

    journal_title：British journal of cancer

    pub_type： 杂志文章,多中心研究

    doi：10.1038/bjc.2014.654

    authors： Bamia C,Lagiou P,Jenab M,Aleksandrova K,Fedirko V,Trichopoulos D,Overvad K,Tjønneland A,Olsen A,Clavel-Chapelon F,Boutron-Ruault MC,Kvaskoff M,Katzke VA,Kühn T,Boeing H,Nöthlings U,Palli D,Sieri S,Panico S,Tumino R

    更新日期：2015-03-31 00:00:00

  • Long term prognosis and quality of life following intensive care for life-threatening complications of haematological malignancy.

    abstract：:Ninety-two consecutive adult patients admitted with acute life-threatening complications of haematological malignancy were studied to determine long term outcome. The quality of life was evaluated in seven long term survivors who are currently alive more than 1 year after hospital discharge using three validated metho...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1991.430

    authors： Yau E,Rohatiner AZ,Lister TA,Hinds CJ

    更新日期：1991-11-01 00:00:00

  • p53 and P-glycoprotein are often co-expressed and are associated with poor prognosis in breast cancer.

    abstract：:Expression of both P-glycoprotein (P-gp) and mutant p53 have recently been reported to be associated with poor prognosis of breast cancer. The expression of P-gp is associated in vitro and in vivo with cross-resistance to several anti-cancer drugs. p53 plays a regulatory role in apoptosis, and mutant p53 has been sugg...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1996.316

    authors： Linn SC,Honkoop AH,Hoekman K,van der Valk P,Pinedo HM,Giaccone G

    更新日期：1996-07-01 00:00:00

  • Cancer risk in Swedish women: the relation to size at birth.

    abstract：:The relationship between fetal growth as indicated by weight and length at birth, and cancer risk in 1080 adult Swedish women was examined. Birth factors were retrieved from original midwife records for the years 1914, 1918, 1922 and 1930, and primary cancer cases were identified by matching with national and regional...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1054/bjoc.2000.1738

    authors： Andersson SW,Bengtsson C,Hallberg L,Lapidus L,Niklasson A,Wallgren A,Hulthén L

    更新日期：2001-05-04 00:00:00

  • Prognostic significance of dihydropyrimidine dehydrogenase expression in breast cancer.

    abstract：:We have investigated dihydropyrimidine dehydrogenase expression as a prognostic marker in breast cancer. A total of 119 women with breast cancer undergoing surgery between 1985 and 1996 were included in this study. Eighty-seven patients were treated with postoperative chemotherapy including 5-fluorouracil or 5-fluorou...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/sj.bjc.6600040

    authors： Horiguchi J,Takei H,Koibuchi Y,Iijima K,Ninomiya J,Uchida K,Ochiai R,Yoshida M,Yokoe T,Iino Y,Morishita Y

    更新日期：2002-01-21 00:00:00

  • Drug transporters: recent advances concerning BCRP and tyrosine kinase inhibitors.

    abstract：:Multidrug resistance is often associated with the (over)expression of drug efflux transporters of the ATP-binding cassette (ABC) protein family. This minireview discusses the role of one selected ABC-transporter family member, the breast cancer resistance protein (BCRP/ABCG2), in the (pre)clinical efficacy of novel ex...

    journal_title：British journal of cancer

    pub_type： 杂志文章,评审

    doi：10.1038/sj.bjc.6604213

    authors： Lemos C,Jansen G,Peters GJ

    更新日期：2008-03-11 00:00:00

  • In utero alcohol exposure increases mammary tumorigenesis in rats.

    abstract：:Findings in humans and animal models suggest that in utero hormonal and dietary exposures increase later breast cancer risk. Since alcohol intake by adult women consistently increases their breast cancer risk, we wondered whether maternal alcohol consumption during pregnancy increases female offspring's mammary tumori...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/sj.bjc.6601793

    authors： Hilakivi-Clarke L,Cabanes A,de Assis S,Wang M,Khan G,Shoemaker WJ,Stevens RG

    更新日期：2004-06-01 00:00:00

  • Case-control study: soft-tissue sarcomas and exposure to phenoxyacetic acids or chlorophenols.

    abstract：:In 1977 a number of patients with soft-tissue sarcomas and previous exposure to phenoxyacetic acids were described. Following from these observations a matched case-control study was made. Exposure to chlorophenols was also included in this study. The results showed that exposure to phenoxyacetic acids or chlorophenol...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1979.125

    authors： Hardell L,Sandström A

    更新日期：1979-06-01 00:00:00

  • Photodynamic destruction of human bladder carcinoma.

    abstract：:Eleven human bladder carcinomata of different degrees of differentiation were implanted in mice immunosuppressed by thymectomy, anti-thymocyte serum and x-rays. Seven carcinomata grew well and one poorly and 3 produced mainly fibrous nodules in the mice. Normal human bladder tissues were grown from 4 other patients. T...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1975.30

    authors： Kelly JF,Snell ME,Berenbaum MC

    更新日期：1975-02-01 00:00:00

  • Mps1 inhibitors synergise with low doses of taxanes in promoting tumour cell death by enhancement of errors in cell division.

    abstract：BACKGROUND:Chromosomal instability (CIN) is a common trait of cancer characterised by the continuous gain and loss of chromosomes during mitosis. Excessive levels of CIN can suppress tumour growth, providing a possible therapeutic strategy. The Mps1/TTK kinase has been one of the prime targets to explore this concept, ...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/s41416-018-0081-2

    authors： Maia ARR,Linder S,Song JY,Vaarting C,Boon U,Pritchard CEJ,Velds A,Huijbers IJ,van Tellingen O,Jonkers J,Medema RH

    更新日期：2018-06-01 00:00:00

  • MicroRNA expression profiling in male and female familial breast cancer.

    abstract：BACKGROUND:Gender-associated epigenetic alterations are poorly investigated in male and female familial breast cancer (fBC). MicroRNAs may contribute to the different biology in men and women particularly related to RASSF1A pathways. METHODS:Microarray technology was used to evaluate miRNA profile in 24 male and 43 fe...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.2014.535

    authors： Pinto R,De Summa S,Danza K,Popescu O,Paradiso A,Micale L,Merla G,Palumbo O,Carella M,Tommasi S

    更新日期：2014-12-09 00:00:00

  • The effect of MDR-1 gene expression on outcome in acute myeloblastic leukaemia.

    abstract：:Resistance to cytotoxic agents may be encountered during the treatment of acute myeloblastic leukaemia (AML). P-glycoprotein encoded by the MDR-1 gene has been implicated as a potential drug resistance mechanism in leukaemic cells. In recent years, many data have been accrued concerning the expression of P-glycoprotei...

    journal_title：British journal of cancer

    pub_type： 杂志文章,评审

    doi：10.1038/bjc.1994.70

    authors： Holmes JA,West RR

    更新日期：1994-02-01 00:00:00