Tyrosine-phosphorylated calmodulin has reduced biological activity.

abstract：:Tobacco-induced oxidative stress leads to chronic inflammation and is implicated in the development of many human epithelial cancers, including head and neck cancer. Cigarette smoke exposure was shown to induce the expression of the ΔNp63α and nitric oxide synthase (NOS)-2 in head and neck squamous cell carcinoma cell...

journal_title：Archives of biochemistry and biophysics

authors： Ratovitski EA

更新日期：2011-02-15 00:00:00

abstract：:Cellobiose dehydrogenase (CDH), an extracellular hemoflavoenzyme produced by the cellulose-degrading cultures of Phanerochaete chrysosporium, oxidizes cellobiose to cellobionolactone. CDH has been purified to homogeneity by a five-step purification procedure. The homogeneous CDH is monomeric and has a relative molecul...

journal_title：Archives of biochemistry and biophysics

authors： Bao W,Usha SN,Renganathan V

更新日期：1993-02-01 00:00:00

abstract：:The mechanism by which chemical inducers of the stress response inhibit protein synthesis was examined. All the chemicals tested principally inhibit the initiation phase of translation. Covalent modification of the initiation factor proteins does not constitute a common mechanism. Eukaryotic initiation factor (eIF)-2 ...

journal_title：Archives of biochemistry and biophysics

authors： Duncan RF,Hershey JW

更新日期：1987-08-01 00:00:00

abstract：:The heme enzyme lignin peroxidase contains a unique Cbeta-hydroxylated tryptophan residue (Trp171) on the surface of the enzyme. Mutagenetic substitution of Trp171 abolishes completely the veratryl alcohol oxidation activity of the enzyme. This led us to surmise that Trp171 may be involved in electron transfer from na...

journal_title：Archives of biochemistry and biophysics

authors： Blodig W,Smith AT,Winterhalter K,Piontek K

更新日期：1999-10-01 00:00:00

abstract：:Two different fractions were present in crystalline bovine liver catalase, and could be resolved using dye-ligand affinity chromatography with Red-A Matrex gel containing Procion HE 3B. The major part (alpha) was not adsorbed on this gel. The second fraction (beta) was firmly adsorbed to the gel, and could be eluted e...

journal_title：Archives of biochemistry and biophysics

authors： Jouve HM,Pelmont J,Gaillard J

更新日期：1986-07-01 00:00:00

abstract：:Rat bone was extracted with KCl and Triton X-100, and a tartrate-resistant acid phosphatase activity was purified by protamine sulfate precipitation, ion-exchange chromatography (CM-cellulose), and gel filtration on Sephadex G-200 according to previously described procedures. Sodium dodecyl sulfate-polyacrylamide gel ...

journal_title：Archives of biochemistry and biophysics

authors： Andersson G,Ek-Rylander B,Hammarström L

更新日期：1984-02-01 00:00:00

abstract：:The in vivo turnover of several rat liver microsomal proteins was studied using techniques designed to maximize antibody recognition specificity and minimize reutilization of radioactive labels. The kinetics of degradation of seven cytochrome P-450 isozymes, NADPH-cytochrome P-450 reductase, and epoxide hydrolase were...

journal_title：Archives of biochemistry and biophysics

authors： Shiraki H,Guengerich FP

更新日期：1984-11-15 00:00:00

abstract：:We measured temporal oscillations of intracellular K+ concentration in yeast cells exhibiting glycolytic oscillations using fluorescence spectroscopy and microscopy methods. These oscillations showed the same period as those of glycolytic metabolites (NADH, ATP), indicating a strong coupling between them. We experimen...

journal_title：Archives of biochemistry and biophysics

authors： Olsen LF,Stock RP,Bagatolli LA

更新日期：2020-03-15 00:00:00

abstract：:Human promatrilysin (matrix metalloproteinase-7) has been produced in Escherichia coli as an N-terminal fusion protein with ubiquitin. The insoluble product was solubilized, refolded, and activated with amino-phenylmercuric acetate. Activation of the fusion protein demonstrated kinetics and intermediates that were ver...

journal_title：Archives of biochemistry and biophysics

authors： Welch AR,Holman CM,Browner MF,Gehring MR,Kan CC,Van Wart HE

更新日期：1995-12-01 00:00:00

abstract：:In the biosynthetic conversion of glutamate to the tetrapyrrole precursor, delta-aminolevulinic acid (ALA), glutamate is activated at C-1 by glutamyl-tRNA synthetase-catalyzed ligation to tRNAGlu. Glutamyl-tRNA reductase next catalyzes reduction of the activated glutamate to glutamate-1-semialdehyde (GSA), which is th...

journal_title：Archives of biochemistry and biophysics

authors： Mayer SM,Rieble S,Beale SI

更新日期：1994-07-01 00:00:00

abstract：:The flavoprotein monoamine oxidase B (MAO B) from bovine liver, as isolated, has an unusual additional absorption band at 412 nm, which is similar to the absorption of its anionic flavin semiquinone form, (Fl.-), and other typical (Fl.-) flavoproteins. Denaturation of the enzyme results in the elimination of this anom...

journal_title：Archives of biochemistry and biophysics

authors： Yue KT,Bhattacharyya AK,Zhelyaskov VR,Edmondson DE

更新日期：1993-01-01 00:00:00

abstract：:A cDNA clone encoding troponin C of Akazara scallop (Chlamys nipponensis akazara) striated adductor muscle was isolated and sequenced. The cDNA is composed of 1987 bp and has an open reading frame of 462 bp at nucleotide positions 12-473. The amino acid sequence deduced from the cDNA showed 89% homology with that of E...

journal_title：Archives of biochemistry and biophysics

authors： Ojima T,Tanaka H,Nishita K

更新日期：1994-06-01 00:00:00

abstract：:Using fractionation procedures such as sucrose gradient sedimentation and gel permeation chromatography, a novel cytosolic binding protein for benzo[a]pyrene has been detected in liver of nonmammalian and mammalian species including human. This protein, called 8 S protein, cosediments with the Ah receptor after centri...

journal_title：Archives of biochemistry and biophysics

authors： Lesca P,Peryt B,Soues S,Maurel P,Cravedi JP

更新日期：1993-05-15 00:00:00

abstract：:The application of 1H-nuclear Overhauser enhancement, 1H-spin-lattice-relaxation-time and 1H-chemical shift measurements for the assessment of the conformational preferences of oligosaccharides are briefly reviewed. It is demonstrated that additivity rules, for the correlation of the chemical shifts of similar hydroge...

journal_title：Archives of biochemistry and biophysics

authors： Lemieux RU,Bock K

更新日期：1983-02-15 00:00:00

abstract：:A cDNA was cloned from Ruta graveolens cells encoding a novel O-methyltransferase (OMT) with high similarity to orcinol or chavicol/eugenol OMTs, but containing a serine-rich N-terminus and a 13 amino acid insertion between motifs IV and V. Expression in Escherichia coli revealed S-adenosyl-l-methionine-dependent OMT ...

journal_title：Archives of biochemistry and biophysics

authors： Burga L,Wellmann F,Lukacin R,Witte S,Schwab W,Schröder J,Matern U

更新日期：2005-08-01 00:00:00

abstract：:Using specific testosterone 16 alpha-hydroxylase activity as the basis for selection of fractions during purification, the cytochrome P-450 ("I"-P-450(16)alpha) has been isolated from livers of phenobarbital-treated 129/J female mice [K. Devore, N. Harada, and M. Negishi (1985) Biochemistry, 24, 5632-5637]. An antibod...

journal_title：Archives of biochemistry and biophysics

authors： Noshiro M,Serabjit-Singh CJ,Bend JR,Negishi M

更新日期：1986-02-01 00:00:00

abstract：:Although differences in size of the right and left thyroid lobes are well defined, differences in morphology, follicles structure, cAMP production, thyrotropin receptor, and protein involved in cell signalling have not previously been reported. This study provides morpho-functional data of right and left thyroid lobes...

journal_title：Archives of biochemistry and biophysics

authors： Albi E,Curcio F,Spelat R,Lazzarini R,Loreti E,Ferri I,Ambesi-Impiombato FS

更新日期：2012-02-01 00:00:00

abstract：:The epithelial cells of the gastrointestinal tract have the capacity to engage in biotransformation of ingested chemicals. A principal component of phase I metabolism of xenobiotics is the family of hemeproteins referred to as cytochrome(s) P450. The presence of cytochrome P450 isoforms was examined by Western blot an...

journal_title：Archives of biochemistry and biophysics

authors： Rosenberg DW

更新日期：1991-01-01 00:00:00

abstract：:Colligin is a collagen-binding glycoprotein localized to the endoplasmic reticulum (ER) and has been proposed to play a role in collagen biosynthesis. Its membership in the serpin family prompted us to examine its effect on procollagen degradation. We first showed that procollagen degradation can take place in the ER ...

journal_title：Archives of biochemistry and biophysics

authors： Jain N,Brickenden A,Ball EH,Sanwal BD

更新日期：1994-10-01 00:00:00

abstract：:Vascular smooth muscle cell (VSMC) proliferation is a key event in the progression of atherosclerosis. Induction of both c-fos (through the transcription factor Elk-1) and c-jun, both immediate early genes, is important for the stimulation of VSMC proliferation and migration. It was earlier found that p38 mitogen-acti...

journal_title：Archives of biochemistry and biophysics

authors： Zhao M,Liu Y,Bao M,Kato Y,Han J,Eaton JW

更新日期：2002-04-15 00:00:00

abstract：:The ability of nine phospholipids to alter the activity of low-Km cyclic AMP phosphodiesterase was examined in microsomal fractions of rat adipocytes. The enzyme was activated by phosphatidylserine (21% at 300 microM) and phosphatidylglycerol (36% at 300 microM). The activation was concentration dependent over the ran...

journal_title：Archives of biochemistry and biophysics

authors： Macaulay SL,Kiechle FL,Jarett L

更新日期：1983-08-01 00:00:00

abstract：:Pyruvate dehydrogenase (E1), an alpha(2)beta(2) tetramer, catalyzes the oxidative decarboxylation of pyruvate and reductive acetylation of lipoyl moieties of the dihydrolipoamide acetyltransferase. The roles of betaW135, alphaP188, alphaM181, alphaH15, and alphaR349 of E1 determined by kinetic analysis were reassessed...

journal_title：Archives of biochemistry and biophysics

authors： Korotchkina LG,Ciszak EM,Patel MS

更新日期：2004-09-15 00:00:00

abstract：:The facultative intracellular bacterial pathogen Listeria monocytogenes invades and multiplies in many mammalian cell types. During the interaction with its host cells it strongly interferes with and modulates host cell functions. In the present review we summarize the current knowledge on the modulation of signal tra...

journal_title：Archives of biochemistry and biophysics

authors： Kuhn M,Pfeuffer T,Greiffenberg L,Goebel W

更新日期：1999-12-01 00:00:00

abstract：:Bacillus stearothermophilus was grown at the optimal temperature range (center, 65 degrees C), below it (48 and 55 degrees C), and above it (68 degrees C), in a complex medium with or without 2.5 mM Ca2+. The Ca(2+)-supplement improves growth at sub- and supraoptimal temperatures and extends it to higher temperatures ...

journal_title：Archives of biochemistry and biophysics

authors： Jurado AS,Pinheiro TJ,Madeira VM

更新日期：1991-08-15 00:00:00

abstract：:Cytosolic proteins may play an important role in the intracellular transport of bile acids in enterocytes. The lithocholate binding properties of cytosolic protein from bovine small intestine were studied. Lithocholate binding was observed in the Y (45-50 kDa), Y' (30-35 kDa), and Z fractions (10-15 kDa) following gel...

journal_title：Archives of biochemistry and biophysics

authors： Takikawa H,Arai S,Yamanaka M

更新日期：1992-11-01 00:00:00

abstract：:Maitotoxin (MTX) is a highly potent marine toxin that activates both voltage-sensitive and receptor-operated calcium channels in the plasma membrane. This results in calcium overload that rapidly leads to cell death. We now report that maitotoxin (0.1-1 nM) induces calpain activation in both SH-SY5Y neuroblastoma cell...

journal_title：Archives of biochemistry and biophysics

authors： Wang KK,Nath R,Raser KJ,Hajimohammadreza I

更新日期：1996-07-15 00:00:00

abstract：:During freezing in sodium and potassium phosphate (NaP and KP) buffer solutions, changes in pH may impact the stability of proteins. Since the degradation pathways for the model proteins, monomeric and tetrameric beta-galactosidase (beta-gal), chosen for this study are governed by conformational changes (i.e., physica...

journal_title：Archives of biochemistry and biophysics

authors： Pikal-Cleland KA,Rodríguez-Hornedo N,Amidon GL,Carpenter JF

更新日期：2000-12-15 00:00:00

abstract：:Activation of the delta-isoform of protein kinase C (deltaPKC) by certain conditions of oxidative stress results in translocation of the kinase to the mitochondria leading to release of cytochrome c and the induction of apoptosis. In the current study, the effects of myocardial reperfusion-induced deltaPKC translocati...

journal_title：Archives of biochemistry and biophysics

authors： Churchill EN,Szweda LI

更新日期：2005-07-15 00:00:00

abstract：:The modes of action of a Vero toxin (VT2 or Shiga-like toxin II) from Escherichia coli, of ricin, and of alpha-sarcin were compared. Elongation factor 1 (EF1) and GTP-dependent Phe-tRNA binding to ribosomes in the presence of poly(U) was inhibited by these three toxins, but EF1 and guanylyl (beta, gamma-methylene)-dip...

journal_title：Archives of biochemistry and biophysics

authors： Furutani M,Kashiwagi K,Ito K,Endo Y,Igarashi K

更新日期：1992-02-14 00:00:00

abstract：:The mechanism of C4 acid decarboxylation was studied in bundle sheath cell strands from Urochloa panicoides, a phosphoenolpyruvate carboxykinase (PCK)-type C4 plant. Added malate was decarboxylated to give pyruvate and this activity was often increased by adding ADP. Added oxaloacetate or aspartate plus 2-oxoglutarate...

journal_title：Archives of biochemistry and biophysics

authors： Burnell JN,Hatch MD

更新日期：1988-01-01 00:00:00