Physiological investigations of a tobacco mutant with o(2)-resistant photosynthesis and enhanced catalase activity.

abstract：:The isotope competition method with glucose-U-(14)C as a carbon source has been used to determine whether or not selected compounds contribute carbon to the biosynthesis of protein amino acids in cells derived from Paul's Scarlet Rose. Of 48 compounds tested, 15 contributed carbon to protein amino acids. The results s...

journal_title：Plant physiology

authors： Dougall DK,Fulton MM

更新日期：1967-07-01 00:00:00

abstract：:Tryptophan decarboxylase (TDC) is a cytosolic enzyme that catalyzes an early step of the terpenoid indole alkaloid biosynthetic pathway by decarboxylation of L-tryptophan to produce the protoalkaloid tryptamine. In the present study, recombinant TDC was targeted to the chloroplast, cytosol, and endoplasmic reticulum (...

journal_title：Plant physiology

authors： Di Fiore S,Li Q,Leech MJ,Schuster F,Emans N,Fischer R,Schillberg S

更新日期：2002-07-01 00:00:00

abstract：:The (13)C nuclear magnetic resonance spectra of sucrose extracted from intact soybean and corn leaves labeled with (13)CO(2) for 5 to 180 minutes have been obtained at 22.6 megahertz. The spectra provide a direct determination of the relative concentrations of (12)C-(13)C and (13)C-(13)C pairs of carbons in the labele...

journal_title：Plant physiology

authors： Schaefer J,Kier LD,Stejskal EO

更新日期：1980-02-01 00:00:00

abstract：:The unfolded protein response (UPR) is activated in plants in response to endoplasmic reticulum stress and plays an important role in mitigating stress damage. Multiple factors act in the UPR, including the membrane-associated transcription factor, BASIC LEUCINE ZIPPER 17 (bZIP17), and the membrane-associated RNA spli...

journal_title：Plant physiology

authors： Bao Y,Bassham DC,Howell SH

更新日期：2019-04-01 00:00:00

abstract：:A method to fractionate corn (Zea mays L. B73) mitochondria into soluble proteins, high molecular weight soluble proteins, and membrane proteins was developed. These fractions were analyzed by both sodium dodecyl sulfate-polyacrylamide gel electrophoresis and assays of mitochondrial enzyme activities. The Krebs cycle ...

journal_title：Plant physiology

authors： Hayes MK,Luethy MH,Elthon TE

更新日期：1991-12-01 00:00:00

abstract：:Phytohemagglutinin, the lectin of the common bean Phaseolus vulgaris, has a high mannose and a modified (fucosylated) oligosaccharide on each polypeptide. Fractionation by high performance liquid chromatography of tryptic digests of [(3)H]fucose or [(3)H]glucosamine labeled phytohemagglutinin, followed by amino acid s...

journal_title：Plant physiology

authors： Sturm A,Chrispeels MJ

更新日期：1986-05-01 00:00:00

abstract：:The effects of the diphenyl ether herbicides HOE 29152 (methyl-2[4-(4-trifluoromethoxy) phenoxy] propanoate) and nitrofluorfen (2-chloro-1-[4-nitrophenoxy]-4-[trifluoromethyl]benzene) on photosynthetic electron transport have been examined with pea seedling and spinach chloroplasts. Linear electron transport (water to...

journal_title：Plant physiology

authors： Bugg MW,Whitmarsh J,Rieck CE,Cohen WS

更新日期：1980-01-01 00:00:00

abstract：:A fructokinase (EC 2.7.1.4) was obtained from pea (Pisum sativum L.) seeds. This enzyme, termed fructokinase (fraction IV), was specific for fructose as substrate and had little activity with glucose or mannose. Excess fructose inhibited the enzyme at the optimum pH (8.2) but not at pH 6.6. MgATP was inhibitory at pH ...

journal_title：Plant physiology

authors： Turner JF,Harrison DD,Copeland L

更新日期：1977-11-01 00:00:00

abstract：:Biochemical and electrophysiological studies on plant vacuolar transporters became feasible in the late 1970s and early 1980s, when methods to isolate large quantities of intact vacuoles and purified vacuolar membrane vesicles were established. However, with the exception of the H+-ATPase and H+-PPase, which could be ...

journal_title：Plant physiology

authors： Martinoia E

更新日期：2018-02-01 00:00:00

abstract：:The functions of mitochondria during leaf senescence, a type of programmed cell death aimed at the massive retrieval of nutrients from the senescing organ to the rest of the plant, remain elusive. Here, combining experimental and analytical approaches, we showed that mitochondrial integrity in Arabidopsis (Arabidopsis...

journal_title：Plant physiology

authors： Chrobok D,Law SR,Brouwer B,Lindén P,Ziolkowska A,Liebsch D,Narsai R,Szal B,Moritz T,Rouhier N,Whelan J,Gardeström P,Keech O

更新日期：2016-12-01 00:00:00

abstract：:In apple (Malus domestica Borkh.) sorbitol is the primary product of photosynthesis, the major translocated form of carbon, and a common fruit constituent and storage compound. Previous work on sorbitol metabolism has revealed a NADPH-dependent aldose 6-phosphate reductase (A6PR) in green tissues, and a NAD-dependent ...

journal_title：Plant physiology

authors： Loescher WH,Marlow GC,Kennedy RA

更新日期：1982-08-01 00:00:00

abstract：:Traditional virus-induced gene silencing (VIGS) is a powerful virus-based short interfering RNA-mediated RNA silencing technique for plant functional genomics. Besides short interfering RNAs, microRNAs (miRNAs) have also been shown to regulate gene expression by RNA silencing in various organisms. However, plant virus...

journal_title：Plant physiology

authors： Tang Y,Wang F,Zhao J,Xie K,Hong Y,Liu Y

更新日期：2010-06-01 00:00:00

abstract：:The light-harvesting chlorophyll protein system of the alga Chlamydobotrys stellata consists of an as yet uncharacterized algal chlorophyll a-protein, called LHCPa, and a common photosystem II-related chlorophyll a/b-protein, called LHCPb (Brandt, Kaiser-Jarry, Wiessner 1982 Biochim Biophys Acta 679: 404-409). For fur...

journal_title：Plant physiology

authors： Brandt P,Zufall E,Wiessner W

更新日期：1983-01-01 00:00:00

abstract：:A new bacterial agglutinin was isolated from seeds of the soybean cultivar Clark. Purification was carried out by ammonium sulfate precipitation and ion-exchange chromatography. The agglutinin is a heat-labile glycoprotein most active at pH 4.0. Addition of Ca(2+), Mn(2+) and Mg(2+) did not enhance the agglutinating a...

journal_title：Plant physiology

authors： Fett WF,Sequeira L

更新日期：1980-11-01 00:00:00

abstract：:We used three-dimensional structured illumination microscopy (3D-SIM) to obtain subdiffraction ("super-resolution") images of plasmodesmata (PD) expressing a green fluorescent protein-tagged viral movement protein (MP) in tobacco (Nicotiana tabacum). In leaf parenchyma cells, we were able to resolve individual compone...

journal_title：Plant physiology

authors： Fitzgibbon J,Bell K,King E,Oparka K

更新日期：2010-08-01 00:00:00

abstract：:The cell walls of wheat (Triticum aestivum) starchy endosperm are dominated by arabinoxylan (AX), accounting for 65% to 70% of the polysaccharide content. Genes within two glycosyl transferase (GT) families, GT43 (IRREGULAR XYLEM9 [IRX9] and IRX14) and GT47 (IRX10), have previously been shown to be involved in the syn...

journal_title：Plant physiology

authors： Lovegrove A,Wilkinson MD,Freeman J,Pellny TK,Tosi P,Saulnier L,Shewry PR,Mitchell RA

更新日期：2013-09-01 00:00:00

abstract：:Auxin-deprived, mannitol-supplemented, suspension-cultured pear (Pyrus communis L. Passe Crassane) fruit cells produce large quantities (20-40 nanoliters ethylene per 10(6) cells per hour) of ethylene in response to auxins, CuCl(2) or 1-amino-cyclopropane-1-carboxylic acid (ACC). Maximum rates of production are achiev...

journal_title：Plant physiology

authors： Puschmann R,Romani R

更新日期：1983-12-01 00:00:00

abstract：:Adenine phosphoribosyltransferase activity was measured in extracts of soybean (Glycine max var. Acme) callus and of senescing barley leaves (Hordeum distichon c.v. Prior). The enzyme from soybean callus had Michaelis constants for adenine and 5-phosphoribosyl pyrophosphate of 1.5 and 7.5 mum respectively and was inhi...

journal_title：Plant physiology

authors： Nicholls PB,Murray AW

更新日期：1968-04-01 00:00:00

abstract：:Barley (Hordeum vulgare L. cv Halcyon) seedlings which had been grown in full strength complete inorganic nutrient media (containing 6 millimolar K(+)) had high internal K(+) concentrations and low values of K(+) ((86)Rb(+)) influx when influx was measured from solutions containing 100 micromolar K(+). Transfer of the...

journal_title：Plant physiology

authors： Fernando M,Kulpa J,Siddiqi MY,Glass AD

更新日期：1990-04-01 00:00:00

abstract：:Cryptogein (CRY), a protein secreted by Phytophthora cryptogea, causes necrosis on tobacco (Nicotiana tabacum) plants at the site of application (the stem or the roots) and also on distant leaves. Autoradiography of plantlets after root absorption of radioiodinated CRY demonstrated a rapid migration of the label to th...

journal_title：Plant physiology

authors： Devergne JC,Bonnet P,Panabières F,Blein JP,Ricci P

更新日期：1992-07-01 00:00:00

abstract：:Surface glandular trichomes distributed throughout the aerial parts of sweet basil (Ocimum basilicum) produce and store monoterpene, sesquiterpene, and phenylpropene volatiles. Three distinct basil chemotypes were used to examine the molecular mechanisms underlying the divergence in their monoterpene and sesquiterpene...

journal_title：Plant physiology

authors： Iijima Y,Davidovich-Rikanati R,Fridman E,Gang DR,Bar E,Lewinsohn E,Pichersky E

更新日期：2004-11-01 00:00:00

abstract：:Phosphoenolpyruvate carboxylase was purified from corn root tips about 80-fold by centrifugation, ammonium sulfate fractionation, and anion exchange and gel filtration chromatography. The resulting preparation was essentially free from malate dehydrogenase, isocitrate dehydrogenase, malate enzyme, NADH oxidase, and py...

journal_title：Plant physiology

authors： Ting IP

更新日期：1968-12-01 00:00:00

abstract：:Mevalonic acid-2-(14)C was readily incorporated into the free, esterified, and glycosidic sterol fractions of tobacco (Nicotiana tabacum L. var. Burley 21) seedlings. The time course of mevalonic acid-2-(14)C incorporation was different for the various individual sterols. Campesterol and sitosterol (group I) became ra...

journal_title：Plant physiology

authors： Bush PB,Grunwald C

更新日期：1973-01-01 00:00:00

abstract：:The breakdown of sucrose to feed both hexoses into glycolytic carbon flow can occur by the sucrose synthase pathway. This uridine diphosphate (UDP) and pyrophosphate (PPi)-dependent pathway was biochemically characterized using soluble extracts from several plants. The sucrolysis process required the simultaneous pres...

journal_title：Plant physiology

authors： Xu DP,Sung SJ,Loboda T,Kormanik PP,Black CC

更新日期：1989-06-01 00:00:00

abstract：:4-Hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) was partially purified from barnyardgrass (Echinochloa crus-galli L.) leaves and assayed by high-performance liquid chromatography analysis of product formation or by the capture of released 14CO2. The bleaching herbicide sulcotrione [2-(2-chloro-4-methanesulfonylben...

journal_title：Plant physiology

authors： Secor J

更新日期：1994-12-01 00:00:00

abstract：:A recognition molecule was isolated from stigmas of S-allele genotype S(2)S(2) of Brassica oleracea var. capitata L. After Sephadex chromatography, it eluted as a single symmetrical peak during diethylaminoethane-cellulose chromatography. A high degree of purity was affirmed by: sedimentation as a single peak during u...

journal_title：Plant physiology

authors： Ferrari TE,Bruns D,Wallace DH

更新日期：1981-02-01 00:00:00

abstract：:The plant-specific Rop subfamily of Rho GTPases, most closely related to the mammalian Cdc42 and Rac GTPases, plays an important role in the regulation of calcium-dependent pollen tube growth, H(2)O(2)-mediated cell death, and many other processes in plants. In a search for Rop interactors using the two-hybrid method,...

journal_title：Plant physiology

authors： Wu G,Li H,Yang Z

更新日期：2000-12-01 00:00:00

abstract：:Isoprenoid biosynthesis in plant plastids occurs via the 1-deoxy-d-xylulose 5-phosphate (DXP) pathway. We used tobacco rattle virus (TRV) to posttranscriptionally silence the expression of the last two enzymes of this pathway, the IspG-encoded (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (HDS) and the IspH-en...

journal_title：Plant physiology

authors： Page JE,Hause G,Raschke M,Gao W,Schmidt J,Zenk MH,Kutchan TM

更新日期：2004-04-01 00:00:00

abstract：:Stomatal closing movements in response to changes from CO(2)-free to CO(2)-containing air were recorded in leaf sections of Zea mays using air flow porometers. The response to CO(2) was fast; the shortest lag between the application of 300 microliters CO(2) per liter of air and the beginning of a stomatal response was...

journal_title：Plant physiology

authors： Raschke K

更新日期：1972-02-01 00:00:00

abstract：:Alder (Alnus glutinosa) and more than 200 angiosperms that encompass 24 genera are collectively called actinorhizal plants. These plants form a symbiotic relationship with the nitrogen-fixing actinomycete Frankia strain HFPArI3. The plants provide the bacteria with carbon sources in exchange for fixed nitrogen, but th...

journal_title：Plant physiology

authors： Jeong J,Suh S,Guan C,Tsay YF,Moran N,Oh CJ,An CS,Demchenko KN,Pawlowski K,Lee Y

更新日期：2004-03-01 00:00:00