Abstract:
:We used three-dimensional structured illumination microscopy (3D-SIM) to obtain subdiffraction ("super-resolution") images of plasmodesmata (PD) expressing a green fluorescent protein-tagged viral movement protein (MP) in tobacco (Nicotiana tabacum). In leaf parenchyma cells, we were able to resolve individual components of PD (neck and central cavities) at twice the resolution of a confocal microscope. Within the phloem, MP-green fluorescent protein filaments extended outward from the specialized pore-PD that connect sieve elements (SEs) with their companion cells (CCs) along the tubular sieve element reticulum (SER). The SER was shown to interconnect individual pore-PD at the SE-CC interface. 3D-SIM resolved fine (less than 100 nm) endoplasmic reticulum threads running into individual pore-PD as well as strands that crossed sieve plate pores, structurally linking SEs within a file. Our data reveal that MP entering the SE from the CC may remain associated with the SER. Fluorescence recovery after photobleaching experiments revealed that this MP pool is relatively immobile compared with the membrane probe 3,3'-dihexyloxacarbocyanine iodide, suggesting that MP may become sequestered by the SER once it has entered the SE. The advent of 3D-SIM offers considerable potential in the subdiffraction imaging of plant cells, bridging an important gap between confocal and electron microscopy.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Fitzgibbon J,Bell K,King E,Oparka Kdoi
10.1104/pp.110.157941subject
Has Abstractpub_date
2010-08-01 00:00:00pages
1453-63issue
4eissn
0032-0889issn
1532-2548pii
pp.110.157941journal_volume
153pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::The photochemistry of vesicular and detergent-solubilized preparations of plasma membrane-associated ATPase was investigated in Rosa damascena. The cholate-solubilized ATPase activity fractionated into two peaks on a Sephadex G-150 column with simple, but different ultraviolet (UV) sensitivities. The larger enzyme was...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.74.3.617
更新日期:1984-03-01 00:00:00
abstract::Ca-mediated processes are known to be involved in transducing many developmental, hormonal, and environmental cues in plant cells. In this study, the role of Ca in the perception of anoxic stress signals by maize (Zea mays L. cv B73) roots was assessed by studying the effect of various Ca antagonists on the induction ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.1.369
更新日期:1994-05-01 00:00:00
abstract::Pectin methylesterase (PME, EC 3.1.1.11) is an ubiquitous enzyme in the plant kingdom; however, its role in plant growth and development is not yet understood. Using transgenic tomato (Lycopersicon esculentum Mill.) fruits that show more than 10-fold reduction in PME activity because of expression of an antisense PME ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.2.429
更新日期:1994-10-01 00:00:00
abstract::Proline content increased greatly in detached rice (Oryza sativa cv. Taichung Native 1) leaves during senescence. There was a slight but significant increase in proline level after one day of incubation, and, subsequently, proline accumulated relatively rapidly. By 4 days after excision, the level of proline had incre...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.6.1348
更新日期:1982-06-01 00:00:00
abstract::Cyanobacteria are oxygenic photosynthetic prokaryotes that are the progenitors of the chloroplasts of algae and plants. These organisms harvest light using large membrane-extrinsic phycobilisome antenna in addition to membrane-bound chlorophyll-containing proteins. Similar to eukaryotic photosynthetic organisms, cyano...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.192849
更新日期:2012-04-01 00:00:00
abstract::The cyanobacterium Agmenellum quadruplicatum PR-6 (Synechococcus sp PCC 7002) was grown turbidostatically in white light at three levels of irradiance: 20, 200, and 1260 microeinsteins per square meter per second. Phycobilisomes were isolated from each culture and analyzed by absorbance, gel electrophoresis, and elect...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.3.1003
更新日期:1992-03-01 00:00:00
abstract::Small GTP-binding proteins regulate diverse processes in eukaryotic cells such as signal transduction, cell proliferation, cytoskeletal organization, and intracellular membrane trafficking. These proteins function as molecular switches that cycle between "active" and "inactive" states, and this cycle is linked to the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.013052
更新日期:2003-03-01 00:00:00
abstract::In this study, we generated transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing the Rieske FeS protein (PetC), a component of the cytochrome b6f (cyt b6f) complex. Increasing the levels of this protein resulted in concomitant increases in the levels of cyt f (PetA) and cyt b6 (PetB), core proteins of t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00622
更新日期:2017-09-01 00:00:00
abstract::Reactive oxygen species (ROS) act as signal molecules for a variety of processes in plants. However, many questions about the roles of ROS in plants remain to be clarified. Here, we report the role of ROS in gibberellin (GA) and abscisic acid (ABA) signaling in barley (Hordeum vulgare) aleurone cells. The production o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.192740
更新日期:2012-04-01 00:00:00
abstract::Key developmental processes that occur on the subcellular and cellular level or occur in occluded tissues are difficult to access, let alone image and analyze. Recently, culturing living samples within polydimethylsiloxane (PDMS) microfluidic devices has facilitated the study of hard-to-reach developmental events. Her...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00879
更新日期:2016-09-01 00:00:00
abstract::A synthetic green fluorescent protein (GFP) gene (pgfp) was constructed to improve GFP expression in plants. Corn and tobacco protoplast transient assays showed that pgfp gave about 20-fold brighter fluorescence than the wild-type gene (gfp). Replacement of the serine at position 65 with a threonine (S65Tpgfp) or a cy...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.3.893
更新日期:1996-11-01 00:00:00
abstract::Brief saturating light flashes were used to probe the mechanism of inactivation of O(2) evolution by Tris in chloroplasts. Maximum inactivation with a single flash and an oscillation with period of four on subsequent flashes was observed. Analyses of the oscillations suggested that only the charge-collecting O(2)-evol...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.4.735
更新日期:1980-04-01 00:00:00
abstract::Root iron mobilization genes are induced by iron deficiency downstream of an unknown signaling mechanism. The FER gene, encoding a basic helix-loop-helix domain protein and putative transcription factor, is required for induction of iron mobilization genes in roots of tomato (Lycopersicon esculentum). To study upstrea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.054270
更新日期:2005-03-01 00:00:00
abstract::Phototropins (phot1 and phot2) are plant blue-light receptors that mediate phototropism, chloroplast movement, stomatal opening, rapid inhibition of growth of etiolated seedlings, and leaf expansion in Arabidopsis (Arabidopsis thaliana). Their N-terminal region contains two light, oxygen, or voltage (LOV) domains, whi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.089839
更新日期:2007-01-01 00:00:00
abstract::Plants, algae, and cyanobacteria have developed mechanisms to decrease the energy arriving at reaction centers to protect themselves from high irradiance. In cyanobacteria, the photoactive Orange Carotenoid Protein (OCP) and the Fluorescence Recovery Protein are essential elements in this mechanism. Absorption of stro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00843
更新日期:2015-09-01 00:00:00
abstract::A culture of Chlorella established by 30-fold dilution of a culture already grown to a level of 15 ml packed cell volume per liter produces little chlorophyll for approximately 12 hours. Investigation of other characteristics such as nitrogen incorporation, increase in packed cell volume and dry weight as well as RNA ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.10.1695
更新日期:1966-12-01 00:00:00
abstract::We have previously identified GAMYB, a gibberellin (GA)-regulated transcriptional activator of alpha-amylase gene expression, in aleurone cells of barley (Hordeum vulgare). To examine the regulation of GAMYB expression, we describe the use of nuclear run-on experiments to show that GA causes a 2-fold increase in the r...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010918
更新日期:2002-05-01 00:00:00
abstract::For the last 50 years, scientists have recognized that varying ratios of the plant hormones cytokinin and auxin induce plant cells to form particular tissues: undifferentiated calli, shoot structures, root structures, or a whole plant. Proliferation of undifferentiated callus tissue, greening, and the formation of sho...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.076059
更新日期:2006-04-01 00:00:00
abstract::Symbioses between plants and mycorrhizal fungi are ubiquitous in ecosystems and strengthen the plants' defense against aboveground herbivores. Here, we studied the underlying regulatory networks and biochemical mechanisms in leaves induced by ectomycorrhizae that modify herbivore interactions. Feeding damage and ovipo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01810
更新日期:2018-04-01 00:00:00
abstract::A combined system has been developed in which epidermal cell turgor, leaf water potential, and gas exchange were determined for transpiring leaves of Tradescantia virginiana L. Uniform and stable values of turgor were observed in epidermal cells (stomatal complex cells were not studied) under stable environmental cond...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.1.66
更新日期:1985-05-01 00:00:00
abstract::Nonhost resistance is defined as the immunity of a plant species to all nonadapted pathogen species. Arabidopsis (Arabidopsis thaliana) ecotype Columbia-0 is nonhost to the oomycete plant pathogen Phytophthora sojae and the fungal plant pathogen Fusarium virguliforme that are pathogenic to soybean (Glycine max). Previ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01982
更新日期:2018-01-01 00:00:00
abstract::Isozymes of glutathione reductase (GR) have been purified from red spruce (Picea rubens Sarg.) needles. Two isozymes could be separated by anion-exchange chromatography from both nonhardened or cold-hardened tissue. Based on chromatographic elution profiles, the isozymes were designated GR-1NH and GR-2NH in preparatio...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.1.205
更新日期:1994-05-01 00:00:00
abstract::Previously we showed that acid invertase activity increased and then decreased rapidly in wounded sweet potato (Ipomoea batatas Liam.) root tissue, and that the tissue contained a heat-stable, proteinaceous inhibitor with a molecular weight of about 19,500 daltons.In response to wounding of sweet potato root tissue, i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.59.5.879
更新日期:1977-05-01 00:00:00
abstract::Transcription of the Arabidopsis thaliana gene encoding ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase (RCA) is organ-specific, light-responsive, and regulated by the circadian clock. RCA is transcribed throughout the green parts of the plant, but not in roots and petals. Responses elicited by shor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.1.43
更新日期:1996-09-01 00:00:00
abstract::The endosperm of 3-day germinated seedlings of Ricinus communis was homogenized in the presence or absence of Mg(2+). When the Mg(2+) -containing homogenate was fractionated on linear, 20 to 40% sucrose gradients, the endoplasmic reticulum (ER) reached equilibrium at a density of 1.146 grams per cubic centimeter. Abse...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.1.83
更新日期:1982-01-01 00:00:00
abstract::DNA methylation plays an important role in diverse developmental processes in many eukaryotes, including the response to environmental stress. Abscisic acid (ABA) is a plant hormone that is up-regulated under stress. The involvement of DNA methylation in the ABA response has been reported but is poorly understood. DNA...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.01471
更新日期:2019-04-01 00:00:00
abstract::Winter- and summertime-active desert annual species were grown at different temperatures to assess their capacity for photosynthetic acclimation. Thermal stability of photosynthesis was determined from responses of chlorophyll fluorescence to increased temperature. Photosynthesis in winter ephemerals grown at 28 degre...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.74.4.786
更新日期:1984-04-01 00:00:00
abstract::When wheat (Triticum aestivum) seedlings are exposed to a cold temperature (2-4 degrees C) above 0 degrees C, sucrose accumulates and sucrose synthase activity increases. The effect of a cold period on the level of sucrose synthase (SS) was investigated. Using antibodies against wheat germ SS, Western blots studies sh...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.3.887
更新日期:1991-07-01 00:00:00
abstract::Highly purified fractions of three starch branching enzymes from developing maize (Zea mays L.) endosperm were used to prepare antisera in rabbits. In double diffusion experiments, no immunoprecipitate was observed when branching enzyme IIa or IIb was tested against branching enzyme I antiserum. No immunoprecipitate w...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.3.813
更新日期:1983-07-01 00:00:00
abstract::The mitochondrial flavoenzyme l-galactono-gamma-lactone dehydrogenase (GALDH) catalyzes the ultimate step of vitamin C biosynthesis in plants. We found that recombinant GALDH from Arabidopsis (Arabidopsis thaliana) is inactivated by hydrogen peroxide due to selective oxidation of cysteine (Cys)-340, located in the cap...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.136929
更新日期:2009-06-01 00:00:00