Abstract:
:In this report we have described several aspects of glial development in cultures containing dissociated DRG neurons and glial cells obtained from dissociated spinal cord at least a week prior to the onset of in vivo myelination. With time in culture the dissociated neurons and glia interact and become organized into 3-dimensional structures possessing many features characteristic of developing CNS in vivo. We have presented evidence that some of the glial cells proliferate and differentiate into galactocerebroside positive (GC+) cells and that some produce myelin sheaths. Thymidine was incorporated into precursors of GC+ cells, but not into cells which were already GC+. Nearly all the astrocytes in areas where neurons were present participated in the formation of large fascicles, and it was within such fascicles that most myelin was formed, suggesting a possible role for astrocytes in creating a favorable microenvironment for myelination. Both the formation of myelin sheaths and the morphological maturity of the oligodendrocytes within the fascicles indicated that oligodendrocyte differentiation proceeded practically to completion in these cultures. In conclusion we believe that the culture system herein described provides an excellent model for in vitro studies of CNS development, while retaining some of the advantages of dissociated cell cultures as well as the possibility of separating and re-uniting the various cell types of interest.
journal_name
Brain Resjournal_title
Brain researchauthors
Wood PM,Williams AKdoi
10.1016/0165-3806(84)90045-2subject
Has Abstractpub_date
1984-02-01 00:00:00pages
225-41issue
2eissn
0006-8993issn
1872-6240journal_volume
314pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::Variables influencing decision-making in real settings, as in the case of voting decisions, are uncontrollable and in many times even unknown to the experimenter. In this case, the experimenter has to study the intention to decide (vote) as close as possible in time to the moment of the real decision (election day). H...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2010.06.046
更新日期:2010-09-10 00:00:00
abstract::Frequency-modulated (FM) sweeps are important components of most natural sounds. To examine the processing of these stimuli we applied a two-tone paradigm. Repeated stimulus presentation usually leads to reduced neuronal responses. However, in a former study repetition enhancements which have been observed when FM ton...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2011.07.009
更新日期:2011-09-09 00:00:00
abstract::Nodes of Ranvier in the central nervous system in mammals are characterized by the presence of perinodal astrocytic processes. This study examines the association between processes of radial glia and the axolemma at nodes of Ranvier in the spinal cord of the mature axolotl, an animal in which radial glia represent a l...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)90595-m
更新日期:1991-05-17 00:00:00
abstract::The number of viable neurones calculated as soon as adult mollusc ganglia tissue was digested and over different periods of cultivation, show that elevated K+ prevents degradation of intercellular contacts, preserves neurones against lysis, prolongs the time course of neurone survival in culture and inhibits neurite f...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90044-0
更新日期:1982-03-18 00:00:00
abstract::The extracellular lactate concentration and blood flow in the cerebral cortex of urethane-anesthetized, paralyzed and artificially ventilated rats were monitored continuously and simultaneously using an enzyme electrode and a laser Doppler flowmeter (LDF), respectively, during chemical stimulation of the nucleus tract...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(96)01428-x
更新日期:1997-05-30 00:00:00
abstract::The rate of 6-[14C]D-glucose oxidation in cerebral cells of SAMP8, a substrain of senescence accelerated mouse, was investigated in vitro. The production of 14CO2 in dissociated intact brain cells prepared from the cerebrum of 4-8-week-old SAMP8 was higher than that of age-matched SAMR2 as a control mouse, while no di...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91226-2
更新日期:1994-02-21 00:00:00
abstract::Dopamine-containing amacrine cells of rhesus monkey were found everywhere outside of the foveola in whole, flat retinas by the formaldehyde-glutaraldehyde fluorescent method. There were about 7500 such cells in a single retina and their density, determined by cell counts and measured by a nearest neighbor method, was ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)91174-0
更新日期:1984-11-19 00:00:00
abstract::We sought to identify the areas that have altered neuronal activity within the hypothalamus of rats with heart failure (HF) by mapping neuronal staining of c-Fos protein (Fos) 6-8 weeks following coronary artery ligation (HF group; n=17) or sham surgery (sham-operated control group, n=15). Fos-like immunoreactivity wa...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02186-7
更新日期:2000-05-19 00:00:00
abstract::The present study was designed to test the hypothesis that a major excitatory mechanism for the expression of feline defensive rage behavior involves the medial nucleus of the amygdala which utilizes substance P as a neurotransmitter in a direct output pathway that supplies the medial hypothalamus. In phase I of the e...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91070-9
更新日期:1993-10-22 00:00:00
abstract::Brainstem noradrenergic neurons located in the A5, A6, and A7 catecholamine cell groups provide the entire noradrenergic innervation of the spinal cord. We have previously demonstrated that noradrenergic neurons in the A6 and A7 cell groups innervate the ventral and dorsal horns, respectively. Since the specific spina...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90210-e
更新日期:1993-07-09 00:00:00
abstract::Antisera against rat-liver S-adenosylhomocysteine hydrolase (SAH-hydrolase) and calf intestinal mucosal adenosine deaminase (ADA) were raised in rabbits and subsequently used to determine the distribution of the corresponding enzymes in rat-brain using the peroxidase-antiperoxidase immunohistochemical procedure. SAH-h...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)90480-4
更新日期:1986-04-09 00:00:00
abstract::The involvement of bulbospinal pathways in the antinociceptive effect of clomipramine in experimental pain was studied. The antinociceptive effect of the antidepressant (0.5 mg/kg), intravenously injected, was evaluated after a unilateral lesion of the dorsolateral funiculus. The results showed that this effect was su...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00826-c
更新日期:1995-10-16 00:00:00
abstract::We have used the post-embedding immunogold labelling method using antibodies to gamma-aminobutyric acid (GABA) to obtain quantitative data on the distribution, frequency, postsynaptic targets and ultrastructural characteristics of GABA-immunoreactive (GABA-IR) boutons in the trigeminal motor nucleus of rats. We have a...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)90757-m
更新日期:1991-10-04 00:00:00
abstract::The effects of the cytokines interleukin-1 and -6 (IL1 beta, IL6; 100 ng) on spatial learning were examined in the Morris water maze. Intracerebroventricular injection of IL1 or IL6 before the training on day 1 did not influence the acquisition of spatial navigation. However, IL1 administered at 60 min, but not immedi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90468-3
更新日期:1993-06-04 00:00:00
abstract::The respiratory function of cerebral mitochondria harvested from genetically diabetic (BB/W) and streptozotocin-diabetic rats deprived of insulin for 3-4 weeks was found to be unchanged from control values. Furthermore, insulin-deprived BB/W rats subjected to 30 min of insulin-induced hypoglycemic coma demonstrated a ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)91624-7
更新日期:1989-02-13 00:00:00
abstract::A monospecific antibody against tyrosine hydroxylase (TH), purified from a transplantable rat pheochromocytoma, was produced in rabbits. Immunohistochemical techniques were employed in order to determine if a relationship exists between the subcellular distribution of TH and the level of activation of the enzyme in th...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)90158-x
更新日期:1981-03-30 00:00:00
abstract::The median preoptic nucleus (MnPN) of the hypothalamus contains sleep-active neurons including sleep-active GABAergic neurons and is involved in the regulation of nonREM/REM sleep. The hypocretinergic (HCRT) neurons of the perifornical-lateral hypothalamic area (PF-LHA) and serotonergic (5-HT) neurons of the dorsal ra...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2008.07.115
更新日期:2008-10-09 00:00:00
abstract::Tyrosine hydroxylase phenotype expression was investigated in the catecholaminergic population of the locus coeruleus neurons of two pure inbred mouse strains, Balb/C and C57Black/6. Therefore, we have characterized the precise organization of tyrosine hydroxylase-expressing perikarya population, in control animals an...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(96)00074-1
更新日期:1996-05-20 00:00:00
abstract::The role of perceptual load in selective attention to multidimensional objects was investigated by independently manipulating the load along the task-relevant and the task-irrelevant dimensions in the central search array, which was flanked by congruent, incongruent, or neutral peripheral distractors. The relative bot...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.07.071
更新日期:2006-10-09 00:00:00
abstract::gamma-L-Glutamyl-L-aspartic acid (gamma-LGLA) has been isolated from Datura stramonium; its structure has been determined and it was then synthesized. In male Swiss mice intraperitoneal administration of the natural peptide (125 nmol/kg) or of the synthetic peptide (25-2500 nmol/kg) 24 h after acquisition of a Y-maze ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)90878-5
更新日期:1988-04-19 00:00:00
abstract::For the evaluation of glutamatergic and GABAergic transmission during seizures, rat hippocampal CA1 and CA3 areas were separately assessed by brain microdialysis, and extracelluar glutamate and GABA were measured through the course of the seizures after a systemic administration of kainic acid (KA). The generalized co...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(98)00507-1
更新日期:1998-07-27 00:00:00
abstract::Circling training during rat striatum postnatal critical period (PN30 to 37 days) induces a life-lasting co-reduction of muscarinic acetylcholine receptors (mAChR) and dopamine D2 receptors (D2R) binding. Here, we evaluated the expression of D1R and D2R under similar experimental conditions. Trained rats showed a decr...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.01.050
更新日期:2004-04-09 00:00:00
abstract::Chick pineal cells contain a circadian oscillator that derives rhythmic synthesis and secretion of melatonin even in dispersed cell culture. Here, we demonstrate that the mRNA encoding tryptophan hydroxylase (TPH), the first enzyme in the melatonin synthetic pathway, is expressed rhythmically under the control of the ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(96)00743-3
更新日期:1996-10-28 00:00:00
abstract::Application of 4-aminopyridine (4-AP) has previously been reported to produce different patterns of epileptiform discharges in entorhinal cortex (EC)-hippocampal slices: recurrent short discharges (RSDs) in hippocampal area CA1, seizure-like events (SLEs) and negative-going potentials (NGPs) in the medial entorhinal c...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)02348-3
更新日期:2000-03-17 00:00:00
abstract::An in vitro method was developed to separately visualize dopaminergic, noradrenergic and serotoninergic terminals in the cerebral, hippocampal and cerebellar cortices of the rat, by means of the glyoxylic histochemical fluorescence method. Animals were pretreated with alpha-methylparatyrosine to deplete catecholamine ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(78)90770-9
更新日期:1978-05-19 00:00:00
abstract::Phosphocreatine-Mg-complex acetate (PCr-Mg-CPLX) is a creatine-derived compound that in previous in vitro research was able to increase neuronal creatine independently of the creatine transporter, thus providing hope to cure the hereditary syndrome of creatine transporter deficiency. In previous research we showed tha...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.06.009
更新日期:2009-08-18 00:00:00
abstract::The telencephalon of teleost fish shows high affinity uptake for D-[3H]aspartate, intermediate levels of GABAergic markers and low levels of cholinergic enzymes. Experimental results (resection of the olfactory tracts or unilateral kainic acid administration in the telencephalon) suggest that: the projection from the ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)90823-6
更新日期:1985-10-07 00:00:00
abstract::The olfactory bulb electroencephalogram (EEG) has been used as a method to imply receptor events12,13. However, experiments to correlate olfactory receptor and bulbar EEG activity in the same species of fish has not been performed. Reported here is the comparison between the simultaneously recorded receptor electroolf...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90170-6
更新日期:1982-10-07 00:00:00
abstract::Anxiety is a very common yet poorly understood symptom of Parkinson's disease. We investigated whether Parkinson's disease patients experiencing anxiety share neural mechanisms described in the general population with involvement of critical regions for the control of behaviour and movement. Thirty-nine patients with ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2020.147235
更新日期:2021-02-15 00:00:00
abstract::Voltage-dependent calcium channels are important for calcium influx and the ensuing intracellular calcium signal in various excitable membranes. The beta subunits of these channels modify calcium currents through pore-forming alpha1 subunits of the high-voltage- activated calcium channels. In the present study, beta3 ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2007.05.041
更新日期:2007-07-30 00:00:00