Tetrahydrobiopterin is synthesized by separate pathways from dihydroneopterin triphosphate and from sepiapterin in adrenal medulla preparations.

abstract：:Solution characterization of heparin with high affinity (HA) and low affinity (LA) for antithrombin III was performed using the methods of small-angle x-ray scattering (SAXS), viscometry, and aqueous gel permeation chromatography (GPC). SAXS provided various topological parameters including the radius of gyration ([S2...

journal_title：Archives of biochemistry and biophysics

authors： Khorramian BA,Stivala SS

更新日期：1986-06-01 00:00:00

abstract：:Serratia rubidaea (ATCC 27614) typifies a substantial number of enteric bacteria which, unlike Escherichia coli, possess a monofunctional species of chorismate mutase (denoted CM-F). CM-F coexists with two additional species of chorismate mutase, each of the latter being one catalytic domain of a bifunctional protein....

journal_title：Archives of biochemistry and biophysics

authors： Xia T,Jensen RA

更新日期：1992-04-01 00:00:00

abstract：:Free radical-mediated reperfusion injury has been established as an important mechanism leading to post-ischemic reperfusion myocardial damage. The present study was undertaken to determine the protective role of vitamin E, a membrane-bound free-radical scavenger, on ischemia-reperfusion myocardial injury. After 4 mon...

journal_title：Archives of biochemistry and biophysics

authors： Pyke DD,Chan AC

更新日期：1990-03-01 00:00:00

abstract：:Myeloperoxidase (MPO), eosinophil peroxidase, lactoperoxidase, and thyroid peroxidase are heme-containing oxidoreductases (EC 1.7.1.11), which bind ligands and/or undergo a series of redox reactions. Though sharing functional and structural homology, reflecting their phylogenetic origin, differences are observed regar...

journal_title：Archives of biochemistry and biophysics

authors： Furtmüller PG,Zederbauer M,Jantschko W,Helm J,Bogner M,Jakopitsch C,Obinger C

更新日期：2006-01-15 00:00:00

abstract：:Ferroptosis is a type of iron-dependent, non-apoptotic cell death, which is typically induced by cysteine starvation or by the inhibition of glutathione peroxidase 4 (GPX4) activity with the accompanying elevation in lipid peroxidation product levels. Despite the central role of mitochondria in oxidative metabolism an...

journal_title：Archives of biochemistry and biophysics

authors： Homma T,Kobayashi S,Sato H,Fujii J

更新日期：2021-01-22 00:00:00

abstract：:The structure of rabbit muscle pyruvate kinase crystallized as a complex with Mg2+, K+, and L-phospholactate (L-P-lactate) has been solved and refined to 2.7 A resolution. The crystals, grown from solutions of polyethylene glycol 8000 at pH 7.5, belong to the space group P2(1) and have unit cell parameters a = 144.4 A...

journal_title：Archives of biochemistry and biophysics

authors： Larsen TM,Benning MM,Wesenberg GE,Rayment I,Reed GH

更新日期：1997-09-15 00:00:00

abstract：:Advancements in peptide fusion technologies to maximize the protein production has taken a big leap to fulfill the demands of post-genomics era targeting elucidation of structure/function of the proteome and its therapeutic applications, by over-expression in heterologous expression systems. Despite being most preferr...

journal_title：Archives of biochemistry and biophysics

authors： Yadav DK,Yadav N,Yadav S,Haque S,Tuteja N

更新日期：2016-12-15 00:00:00

abstract：:Cytochrome P450 reductase was purified to homogeneity from cell suspension cultures of the opium poppy Papaver somniferum, the enzyme was characterized (K(m) cytochrome c, 8.3 microM; K(m) NADPH, 4.2 microM; pH optimum, 8.0; M(r), 80 kDa), and the amino acid sequence of internal peptides was determined. Partial cDNA c...

journal_title：Archives of biochemistry and biophysics

authors： Rosco A,Pauli HH,Priesner W,Kutchan TM

更新日期：1997-12-15 00:00:00

abstract：:Human cytochrome CYP3A4 is the most abundant of all the P450s in human liver and is involved in the metabolism of many environmental toxicants and drugs. Kinetic studies with CYP3A4 have been hampered due to low activity of this enzyme obtained from recombinant gene expression systems or difficulty in reconstituting a...

journal_title：Archives of biochemistry and biophysics

authors： Lee CA,Kadwell SH,Kost TA,Serabjit-Singh CJ

更新日期：1995-05-10 00:00:00

abstract：:Recently, we synthesized and characterized vanadyl saccharides to evaluate the effects of various vanadate and vanadyl complexes, which differ in their oxidation states on various biomacromolecules and cellular activities (1, 2). Here, we report that both vanadate (+V oxidation state) and different vanadyl species (+I...

journal_title：Archives of biochemistry and biophysics

authors： Krishnamoorthy T,Sreedhara A,Rao CP,Ramaiah KV

更新日期：1998-01-01 00:00:00

abstract：:Binding of the mouse IgM antibody 6C4 is lost after treatment of human free secretory component with peptide N-glycosidase F (Bakos et al. (1991) J. Immunol. 146, 162-168) or periodate, suggesting that asparagine-linked oligosaccharides contain the epitope recognized by this antibody. Inhibition of antibody binding to...

journal_title：Archives of biochemistry and biophysics

authors： Yu H,Sipes JM,Cashel J,Bakos MA,Goldblum RM,Roberts DD

更新日期：1995-10-01 00:00:00

abstract：:Protoporphyrinogen oxidase (EC 1.3.3.4) is the penultimate enzyme in the heme biosynthetic pathway, catalyzing the six-electron oxidation of protoporphyrinogen to protoporphyrin. A dominantly inherited genetic deficiency in this enzyme results in the disease variegate porphyria. We now report the cloning, sequence, an...

journal_title：Archives of biochemistry and biophysics

authors： Dailey TA,Dailey HA,Meissner P,Prasad AR

更新日期：1995-12-20 00:00:00

abstract：:Metabolomics, the characterization of the set of small molecules in a biological system, is advancing research in multiple areas of islet biology. Measuring a breadth of metabolites simultaneously provides a broad perspective on metabolic changes as the islets respond dynamically to metabolic fuels, hormones, or envir...

journal_title：Archives of biochemistry and biophysics

authors： Gooding JR,Jensen MV,Newgard CB

更新日期：2016-01-01 00:00:00

abstract：:The oxidizing power of the thiyl radical (GS*) produced on oxidation of glutathione (GSH) was determined as the mid-point electrode potential (reduction potential) of the one-electron couple E(m)(GS*,H+/GSH) in water, as a function of pH over the physiological range. The method involved measuring the equilibrium const...

journal_title：Archives of biochemistry and biophysics

authors： Madej E,Wardman P

更新日期：2007-06-01 00:00:00

abstract：:It was shown previously that the ATP synthase complex of bovine heart mitochondria contains an essential set of thiols or dithiols in its membrane sector (F0), whose modification by various reagents results in uncoupling [Yagi, T., and Hatefi, Y. (1984) Biochemistry 23, 2449-2455]. The sensitivity to modifiers was inc...

journal_title：Archives of biochemistry and biophysics

authors： Yagi T,Hatefi Y

更新日期：1987-04-01 00:00:00

abstract：:A class II Zn(2+)-dependent fructose-1,6-bisphosphate (FBP)- aldolase was purified from an overproducer strain of Escherichia coli and characterized by standard biochemical techniques and 13C NMR spectroscopy. The principal finding of these studies was identification, by 13C NMR spectroscopy, of an enzyme-bound reacti...

journal_title：Archives of biochemistry and biophysics

authors： Szwergold BS,Ugurbil K,Brown TR

更新日期：1995-02-20 00:00:00

abstract：:hCD157 catalyzes the hydrolysis of nicotinamide riboside (NR) and nicotinic acid riboside (NAR). The release of nicotinamide or nicotinic acid from NR or NAR was confirmed by spectrophotometric, HPLC and NMR analyses. hCD157 is inactivated by a mechanism-based inhibitor, 2'-deoxy-2'-fluoro-nicotinamide arabinoside (fN...

journal_title：Archives of biochemistry and biophysics

authors： Preugschat F,Carter LH,Boros EE,Porter DJ,Stewart EL,Shewchuk LM

更新日期：2014-12-15 00:00:00

abstract：:Protein synthesis and lipid peroxidation were evaluated in rat liver slices incubated in the presence of oxidants and protein synthesis inhibitors. Protein synthesis by rat liver slices was evaluated by [3H]leucine incorporation into the trichloroacetic acid (TCA)-insoluble material, and lipid peroxidation was evaluat...

journal_title：Archives of biochemistry and biophysics

authors： Fraga CG,Zamora R,Tappel AL

更新日期：1989-04-01 00:00:00

abstract：:Recently our group synthesized a new class of melanins obtained by the tyrosinase-catalyzed oxidation of opioid peptides (opiomelanins). Owing to the presence of the peptide moiety such pigments exhibit high solubility in hydrophilic solvents, which allows spectroscopic investigations. In particular, the absence of so...

journal_title：Archives of biochemistry and biophysics

authors： Mosca L,De Marco C,Fontana M,Rosei MA

更新日期：1999-11-01 00:00:00

abstract：:The role of sarcoplasmic reticulum (SR) in malignant hyperthermia (MH) was studied using the heavy microsomal fraction prepared from semitendinosus muscles of both normal and genetically MH-susceptible pigs. In the presence of ATP, SR was loaded with 70 nmol Ca2+/mg SR protein. Under these conditions, MH-SR demonstrat...

journal_title：Archives of biochemistry and biophysics

authors： Ohnishi ST,Waring AJ,Fang SR,Horiuchi K,Flick JL,Sadanaga KK,Ohnishi T

更新日期：1986-06-01 00:00:00

abstract：:Preparations of one-chain tissue plasminogen activator (tPA), usually a mixture of 65- and 63-kDa differentially glycosylated forms, contain variable amounts of two-chain tPA. There is no effective procedure currently available for removal of the two-chain contaminant from one-chain tPA preparations. In this report, a...

journal_title：Archives of biochemistry and biophysics

authors： Husain SS

更新日期：1991-03-01 00:00:00

abstract：:An in vitro acrosome-like reaction was induced in spermatozoa from the boar cauda epididymis by incubation in Tyrode's solution containing 1 mg/ml fatty acid-free bovine serum albumin. Plasma membranes were isolated from the spermatozoa at different times during the incubation and analyzed for their lipid composition....

journal_title：Archives of biochemistry and biophysics

authors： Nikolopoulou M,Soucek DA,Vary JC

更新日期：1986-10-01 00:00:00

abstract：:Thromboxane A2 synthase (TXAS) binds to the endoplasmic reticulum membrane and catalyzes both an isomerization of prostaglandin H2 (PGH2) to form thromboxane A2 (TXA2) and a fragmentation of PGH2 to form 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT) and malondialdehyde (MDA). TXAS is a non-classic cytochrome P450 in ...

journal_title：Archives of biochemistry and biophysics

authors： Hsu PY,Wang LH

更新日期：2003-08-01 00:00:00

abstract：:Unlike monooxygenases, molybdenum-containing hydroxylases catalyze the hydroxylation of carbon centers using oxygen derived ultimately from water, rather than O(2), as the source of the oxygen atom incorporated into the product, and do not require an external source of reducing equivalents. The mechanism by which this...

journal_title：Archives of biochemistry and biophysics

authors： Hille R

更新日期：2005-01-01 00:00:00

abstract：:Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase [ATP:oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.49] is completely inactivated by the 2',3'-dialdehyde derivative of ATP (oATP) in the presence of Mn2+. The dependence of the pseudo-first-order rate constant on reagent concentration indicates t...

journal_title：Archives of biochemistry and biophysics

authors： Saavedra C,Araneda S,Cardemil E

更新日期：1988-11-15 00:00:00

abstract：:Magnesium is involved in a wide range of biochemical reactions that are crucial to cell proliferation, differentiation, angiogenesis, and apoptosis. Changes in magnesium availability have been shown to influence biological responses of immuno-inflammatory cells. Equally plausible seems to be an involvement of magnesiu...

journal_title：Archives of biochemistry and biophysics

authors： Wolf FI,Maier JA,Nasulewicz A,Feillet-Coudray C,Simonacci M,Mazur A,Cittadini A

更新日期：2007-02-01 00:00:00

abstract：:Over the past three decades the protein folding field has undergone monumental changes. Originally a purely academic question, how a protein folds has now become vital in understanding diseases and our abilities to rationally manipulate cellular life by engineering protein folding pathways. We review and contrast past...

journal_title：Archives of biochemistry and biophysics

authors： Chen Y,Ding F,Nie H,Serohijos AW,Sharma S,Wilcox KC,Yin S,Dokholyan NV

更新日期：2008-01-01 00:00:00

abstract：:Glycosylasparaginase (EC 3.5.1.26) is a lysosomal amidase which hydrolyzes the bond between asparagine and the sugar moiety in N-linked glycoproteins. Deficiency of the enzyme results in aspartylglycosaminuria (AGU), the most common disorder of glycoprotein degradation. Mature enzyme is formed by two proteolytic cleav...

journal_title：Archives of biochemistry and biophysics

authors： Park H,Vettese-Dadey M,Aronson NN Jr

更新日期：1996-04-01 00:00:00

abstract：:Inducible formation of ribulose-1,5-bisphosphate (RuBP) carboxylase in the cells of Chromatium vinosum under autotrophic conditions was not affected by six different inhibitors of DNA synthesis. Photosynthetic CO2 fixation and RuBP carboxylase activities were not influenced by seven reagents known to eliminate plasmid...

journal_title：Archives of biochemistry and biophysics

authors： Kobayashi H,Akazawa T

更新日期：1983-07-01 00:00:00

abstract：:Total liver poly(A+)-RNA isolated from untreated, and 3-methylcholanthrene-, trans-stilbene oxide-, and phenobarbital-treated rats has been translated in the rabbit reticulocyte lysate system in order to determine the effect of these xenobiotics on the level of translationally active DT-diaphorase mRNA. The in vitro t...

journal_title：Archives of biochemistry and biophysics

authors： Williams JB,Wang R,Lu AY,Pickett CB

更新日期：1984-07-01 00:00:00