Metabolomics applied to the pancreatic islet.

abstract：:Diglycyl triperoxodivanadate [V2O2(O2)3(Gly H)2(H2O)2], a synthetic compound with mu-peroxo-bridge derived from H2O2 and vanadate, oxidized bromide to a bromination-competent intermediate in phosphate buffer and physiological pH. This is in contrast to the requirement of acid medium with H2O2 as the oxidant. Addition ...

journal_title：Archives of biochemistry and biophysics

authors： Rao AV,Islam NS,Ramasarma T

更新日期：1997-06-15 00:00:00

abstract：:In spite of the abundance of antifungal therapies, 75% of women in the world suffer from the second most common cause of vaginal infection named vulvovaginal candidiasis. This complication is characterized with overgrowth of Candida albicans. The low efficacy and side effects of current antifungal therapies have convi...

journal_title：Archives of biochemistry and biophysics

authors： Ebrahimi-Shaghaghi F,Noormohammadi Z,Atyabi SM,Razzaghi-Abyaneh M

更新日期：2021-01-22 00:00:00

abstract：:Atroxase, isolated from the venom of Crotalus atrox (western diamondback rattlesnake), is a nonhemorrhagic protease which has fibrinolytic activity in vivo. The primary structure of atroxase was deduced from the cDNA encoding the atroxase protein. The venom glands of Crotalus atrox were used to prepare a cDNA library....

journal_title：Archives of biochemistry and biophysics

authors： Baker BJ,Wongvibulsin S,Nyborg J,Tu AT

更新日期：1995-03-10 00:00:00

abstract：:A series of novel peptidyl-alpha-ketoamide compounds were evaluated as inhibitors of the deltaNS3-NS4A serine protease from the hepatitis C virus. These peptidyl-alpha-ketoamide inhibitors with Ki values ranging from 0.17 nM to 5.6 microM exhibited slow-binding inhibition. Kinetic studies established one-step kinetic ...

journal_title：Archives of biochemistry and biophysics

authors： Liu Y,Stoll VS,Richardson PL,Saldivar A,Klaus JL,Molla A,Kohlbrenner W,Kati WM

更新日期：2004-01-15 00:00:00

abstract：:Helix 7 in the Cry4Ba-pore-forming domain contains conserved Tyr(249) and Phe(264) that are crucially involved in mosquito-larvicidal activity. We have now characterized lipid-induced conformation of a 27-residue Cry4Ba-alpha7 peptide in phospholipid membranes using ATR-FTIR and hydrogen/deuterium (H(+)/D(+)) exchange...

journal_title：Archives of biochemistry and biophysics

authors： Tiewsiri K,Fischer WB,Angsuthanasombat C

更新日期：2009-02-01 00:00:00

abstract：:The role of fatty acid metabolism in chemical-dependent cell injury is poorly understood. Addition of L-carnitine to the incubation medium of cultured hepatocytes delayed cell killing initiated by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Protection by L-carnitine was stereospecific and observed as late as ...

journal_title：Archives of biochemistry and biophysics

authors： Snyder JW,Kyle ME,Ferraro TN

更新日期：1990-01-01 00:00:00

abstract：:The reaction by which the antioxidant capacity of alpha-tocopherol is sustained by recycling of the alpha-tocopheroxyl radical in membranes or lipoproteins by aqueous ascorbate has been studied by laser flash photolysis in model micellar and membrane systems. In bilayers of dimyristoylphosphatidylcholine at 35 degrees...

journal_title：Archives of biochemistry and biophysics

authors： Bisby RH,Parker AW

更新日期：1995-02-20 00:00:00

abstract：:Some compounds are known to induce solute-nonselective permeability of the inner mitochondrial membrane (IMM) in Ca2+-loaded mitochondria. Existing data suggest that this process, following the opening of a mitochondrial permeability transition pore, is preceded by different solute-selective permeable states of IMM. A...

journal_title：Archives of biochemistry and biophysics

authors： Balakirev MY,Zimmer G

更新日期：1998-08-01 00:00:00

abstract：:The activity of thymidylate synthetase in the liver of the ddY strain male mouse increased transitorily according to the increase in tumor cell number at maximum 7-9 days after ip transplantation of Ehrlich ascites tumor. The enzyme was able to be purified from the tumor host mouse liver or from the normal mouse liver...

journal_title：Archives of biochemistry and biophysics

authors： Asanagi M,Moriyama Y,Fujimura S

更新日期：1988-12-01 00:00:00

abstract：:It is widely accepted that the function of human renalase is to oxidize catecholamines in blood. However, this belief is based on experiments that did not account for slow, facile catecholamine autoxidation reactions. Recent evidence has shown that renalase has substrates with which it reacts rapidly. The reaction cat...

journal_title：Archives of biochemistry and biophysics

authors： Beaupre BA,Hoag MR,Moran GR

更新日期：2015-08-01 00:00:00

abstract：:Piratoxins (PrTX) I and III are phospholipases A2 (PLA2s) or PLA2 homologue myotoxins isolated from Bothrops pirajai snake venom, which also induce myonecrosis, bactericidal activity against Escherichia coli, disruption of artificial membranes, and edema. PrTX-III is a catalytically active hemolytic and anticoagulant ...

journal_title：Archives of biochemistry and biophysics

authors： Soares AM,Andrião-Escarso SH,Bortoleto RK,Rodrigues-Simioni L,Arni RK,Ward RJ,Gutiérrez JM,Giglio JR

更新日期：2001-03-15 00:00:00

abstract：:The solution structure of native and systematically modified ovine submaxillary mucin (OSM) has been probed by proton NMR spectroscopic methods. Most of the resonances in the spectra have been tentatively assigned to the peptide and O-linked disaccharide, alpha-N-acetylneuraminic acid 2----6 alpha-N-acetylgalactosamin...

journal_title：Archives of biochemistry and biophysics

authors： Gerken TA

更新日期：1986-06-01 00:00:00

abstract：:Flavopiridol has been shown to induce cell cycle arrest and apoptosis in various tumor cells in vitro and in vivo. Using immobilized flavopiridol, we identified glycogen phosphorylases (GP) from liver and brain as flavopiridol binding proteins from HeLa cell extract. Purified rabbit muscle GP also bound to the flavopi...

journal_title：Archives of biochemistry and biophysics

authors： Kaiser A,Nishi K,Gorin FA,Walsh DA,Bradbury EM,Schnier JB

更新日期：2001-02-15 00:00:00

abstract：:The formation of alpha-hydroxyethyl radical from ethanol by deermouse microsomes supplemented with NADPH has been demonstrated with the EPR technique of spin trapping with alpha-(4-pyridyl-1-oxide)-N-t-butylnitrone (POBN) as the spin trap, in the presence of deferoxamine mesylate. Superoxide dismutase prevented the fo...

journal_title：Archives of biochemistry and biophysics

authors： Knecht KT,Thurman RG,Mason RP

更新日期：1993-06-01 00:00:00

abstract：:Glyceraldehyde (GA) has been used to study insulin secretion for decades and it is widely assumed that beta-cell metabolism of GA after its phosphorylation by triokinase is similar to metabolism of glucose; that is metabolism through distal glycolysis and oxidation in mitochondria. New data supported by existing infor...

journal_title：Archives of biochemistry and biophysics

authors： MacDonald MJ,Chaplen FW,Triplett CK,Gong Q,Drought H

更新日期：2006-03-15 00:00:00

abstract：:Current issues in research on health effects by polyphenols are addressed. As to the cardiovascular system, flow-mediated dilation (FMD), a functional biomarker, can be used as surrogate marker for cardiovascular risk. Acute short-term effects peaking at 2h after ingestion of polyphenol-rich food items are distinguish...

journal_title：Archives of biochemistry and biophysics

authors： Sies H

更新日期：2010-09-01 00:00:00

abstract：:Uridine kinase is the rate-limiting enzyme in the pyrimidine salvage pathway of all mammalian cells. A cDNA for uridine kinase from mouse brain has been isolated, sequenced, and characterized. This is the first report of a complete nucleotide sequence for mammalian uridine kinase. The isolated cDNA is only 95% complet...

journal_title：Archives of biochemistry and biophysics

authors： Ropp PA,Traut TW

更新日期：1996-12-01 00:00:00

abstract：:The role of chalcone synthase in the regulation of flavonoid biosynthesis during organogenesis of oat primary leaves has been investigated at the level of enzyme activity and mRNA translation in vitro. Chalcone synthase was purified about 500-fold. The apparent Km values were 1.5 and 6.3 microM for 4-coumaroyl-CoA and...

journal_title：Archives of biochemistry and biophysics

authors： Knogge W,Schmelzer E,Weissenböck G

更新日期：1986-11-01 00:00:00

abstract：:Bovine heart muscle microsomes rapidly convert lysophosphatidylcholine (LPC) into phosphatidylcholine (PC) in the presence of oleoyl-CoA. Both substrates are incorporated into the product, although the rate of incorporation of radiolabel into PC from 1-[14C]palmitoyl-LPC was approximately threefold higher than the rat...

journal_title：Archives of biochemistry and biophysics

authors： Sanjanwala M,Sun GY,Cutrera MA,MacQuarrie RA

更新日期：1988-09-01 00:00:00

abstract：:A series of peptidyl alpha-keto esters, alpha-keto amides, alpha-keto acids, and alpha-diketones were synthesized which reversibly inhibit papain and cathepsin B. Methyl 3-(N-benzyloxycarbonyl-L-phenylalanyl)amino-2-oxopropionate (a dicarbonyl compound) inhibits papain with a Ki value of 1 microM, whereas the Ki of 3-...

journal_title：Archives of biochemistry and biophysics

authors： Hu LY,Abeles RH

更新日期：1990-09-01 00:00:00

abstract：:A comparative study was made of the metal ion requirement of rat liver mitochondrial phospholipase A2 in purified and membrane-associated forms. Membrane-bound enzyme was assayed using either exogenous or endogenous phosphatidylethanolamine. Although several divalent metal ions caused increased activity of the membran...

journal_title：Archives of biochemistry and biophysics

authors： De Winter JM,Korpancova J,van den Bosch H

更新日期：1984-10-01 00:00:00

abstract：:An increased permeability of the intestinal barrier is proposed as a major event in the pathophysiology of inflammatory bowel diseases (IBD). Tumor necrosis alpha (TNFα) plays a central role in IBD pathogenesis, in part promoting tight function (TJ) barrier dysfunction. Food extracts enriched in (-)-epicatechin (EC) p...

journal_title：Archives of biochemistry and biophysics

authors： Contreras TC,Ricciardi E,Cremonini E,Oteiza PI

更新日期：2015-05-01 00:00:00

abstract：:African trypanosomes of the Trypanosoma brucei group are agents of disease in man and animals. They present unique biochemical characteristics such as the need for preformed purines and have extensive salvage mechanisms for nucleoside recovery. In this regard we have shown that trypanosomes have a dedicated transporte...

journal_title：Archives of biochemistry and biophysics

authors： Goldberg B,Rattendi D,Lloyd D,Yarlett N,Bacchi CJ

更新日期：1999-04-01 00:00:00

abstract：:The relationship between prolonged exercise, oxidative stress, and the protective capacity of the antioxidant defense system has been determined. Venous blood samples were removed from seven trained athletes before and up to 120 h after completion of a half-marathon for measurements of blood antioxidants, antioxidant ...

journal_title：Archives of biochemistry and biophysics

authors： Duthie GG,Robertson JD,Maughan RJ,Morrice PC

更新日期：1990-10-01 00:00:00

abstract：:Reversible oxidation of Cys residues is a crucial element of redox homeostasis and signaling. According to a popular concept in oxidative stress signaling, the oxidation of targets of signals can only take place following an overwhelming of the cellular antioxidant capacity. This concept, however, ignores the activati...

journal_title：Archives of biochemistry and biophysics

authors： Zaccarin M,Bosello-Travain V,Di Paolo ML,Falda M,Maiorino M,Miotto G,Piccolo S,Roveri A,Ursini F,Venerando R,Toppo S

更新日期：2017-03-01 00:00:00

abstract：:Myosin prepared from tilapia (Serotherodon aureus) was complexed with 8-anilino-1-naphthalene sulfonate (ANS) and continuously heated at 1 degree C/min. A large increase in fluorescence was observed with a transition temperature of 34 degrees C. The effect of several salts on the transition temperature was tested. A p...

journal_title：Archives of biochemistry and biophysics

authors： Wicker L,Knopp JA

更新日期：1988-11-01 00:00:00

abstract：:Imidazolium trans-imidazoledimethylsulfoxide-tetrachlororuthenate (NAMI-A) is a new ruthenium compound active against lung metastasis in vivo and tumor cell invasion in vitro. Since angiogenesis was recognized as a key event in the metastasizing process, the manipulation of neo-vessel formation has been developed as a...

journal_title：Archives of biochemistry and biophysics

authors： Sanna B,Debidda M,Pintus G,Tadolini B,Posadino AM,Bennardini F,Sava G,Ventura C

更新日期：2002-07-15 00:00:00

abstract：:In contrast to other P450 enzymes purified from rat liver microsomes, purified P450 IIIA1 (P450p) is catalytically inactive when reconstituted with NADPH-cytochrome P450 reductase and the synthetic lipid, dilauroylphosphatidylcholine. However, purified P450 IIIA1 catalyzes the oxidation of testosterone when reconstitu...

journal_title：Archives of biochemistry and biophysics

authors： Eberhart DC,Parkinson A

更新日期：1991-12-01 00:00:00

abstract：:The St-20 and ST-40 cDNAs encode rat liver hydroxysteroid sulfotransferases (HS-ST) that are 90% identical in amino acid sequence but exhibit different substrate preferences for dehydroepiandrosterone (DHEA), androsterone (AD), and cortisol (CS). ST-40 is active for all three substrates, whereas ST-20 is mainly active...

journal_title：Archives of biochemistry and biophysics

authors： Tamura H,Morioka Y,Homma H,Matsui M

更新日期：1997-05-15 00:00:00

abstract：:The effects of benzyl (BITC) and phenethyl isothiocyanate (PEITC) on the activity of a P450 2E1 mutant where the conserved threonine at position 303 was replaced with an alanine residue (P450 2E1 T303A) were examined. PEITC inactivated the mutant enzyme with a K(I) of 1.6 microM. PEITC also inactivated the wild-type P...

journal_title：Archives of biochemistry and biophysics

authors： Moreno RL,Goosen T,Kent UM,Chung FL,Hollenberg PF

更新日期：2001-07-01 00:00:00